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The map label for this gene is spoT [H]

Identifier: 113475732

GI number: 113475732

Start: 3247778

End: 3248368

Strand: Reverse

Name: spoT [H]

Synonym: Tery_2079

Alternate gene names: 113475732

Gene position: 3248368-3247778 (Counterclockwise)

Preceding gene: 113475734

Following gene: 113475730

Centisome position: 41.91

GC content: 42.47

Gene sequence:

>591_bases
ATGATGCTTTCGCTCCATTTTACACAAGCTTTAGTATACGCCGCGGAGTTACACGCTGATCAAGTTCGCAAAGGAAGTAA
GGTTCCTTATATTTCTCACCTTTTGGGGGTGGCTAGCATTGCCCTCGAGTATGGCGCTGATGAAGATGAAGCTATAGCAG
CATTGCTCCACGATGCTATAGAAGATCAAGGTGGGGAAACTGTTCGTGGAGAAATTAGAGCACGTTTTGGGGAGAGAGTG
ACTGAAATAGTGGATGGTTGCACTGAAACGGATATTACTCCTAAACCTCCTTGGGCAGAACGTAAAAAAGCTTATATTGC
TCGTCTTGCTAATGCTTCACCTTCAGTACGCTTGGTTTCTTTAGCAGATAAACTACATAATGTGAGGTCTATTTTAAAAG
ACTATCGGACCCAAGGTGATGTACTTTGGAATATATTTAAGGGTGGTAAAGAAGGTACGCTCTGGTATTATCGATCTCTT
GTGACTGCATTTCATCAGGCTGGCTCTACTCCGATGGTAGTAGAGTTAGATCGTTTAGTTACAGAATTAGAAGGGTTAGT
TGAGATAGATATGGCTCCAACTGTTGGTTAA

Upstream 100 bases:

>100_bases
GTTGAATACAAGGGCAAAATAATCTATTATTGTTGCACTAAAAAGTGACAGATTGATTTTTGCTCTATATTGTAATTTTT
TTTAACTTTTGACTAAAAAA

Downstream 100 bases:

>100_bases
GGTTGTTTCCCTGGCCGCTTCAGTTGTTAGTTAATGTTTTTATTAAATTAAACTAACTGCTTGACCCGAAATTGTTCTAG
TTCTCCTGGGAGAACTGTAC

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MMLSLHFTQALVYAAELHADQVRKGSKVPYISHLLGVASIALEYGADEDEAIAALLHDAIEDQGGETVRGEIRARFGERV
TEIVDGCTETDITPKPPWAERKKAYIARLANASPSVRLVSLADKLHNVRSILKDYRTQGDVLWNIFKGGKEGTLWYYRSL
VTAFHQAGSTPMVVELDRLVTELEGLVEIDMAPTVG

Sequences:

>Translated_196_residues
MMLSLHFTQALVYAAELHADQVRKGSKVPYISHLLGVASIALEYGADEDEAIAALLHDAIEDQGGETVRGEIRARFGERV
TEIVDGCTETDITPKPPWAERKKAYIARLANASPSVRLVSLADKLHNVRSILKDYRTQGDVLWNIFKGGKEGTLWYYRSL
VTAFHQAGSTPMVVELDRLVTELEGLVEIDMAPTVG
>Mature_196_residues
MMLSLHFTQALVYAAELHADQVRKGSKVPYISHLLGVASIALEYGADEDEAIAALLHDAIEDQGGETVRGEIRARFGERV
TEIVDGCTETDITPKPPWAERKKAYIARLANASPSVRLVSLADKLHNVRSILKDYRTQGDVLWNIFKGGKEGTLWYYRSL
VTAFHQAGSTPMVVELDRLVTELEGLVEIDMAPTVG

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=126, Percent_Identity=37.3015873015873, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25143118, Length=125, Percent_Identity=38.4, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650996, Length=131, Percent_Identity=34.3511450381679, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 21635; Mature: 21635

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLSLHFTQALVYAAELHADQVRKGSKVPYISHLLGVASIALEYGADEDEAIAALLHDAI
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EDQGGETVRGEIRARFGERVTEIVDGCTETDITPKPPWAERKKAYIARLANASPSVRLVS
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHH
LADKLHNVRSILKDYRTQGDVLWNIFKGGKEGTLWYYRSLVTAFHQAGSTPMVVELDRLV
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHH
TELEGLVEIDMAPTVG
HHHHHHEEECCCCCCC
>Mature Secondary Structure
MMLSLHFTQALVYAAELHADQVRKGSKVPYISHLLGVASIALEYGADEDEAIAALLHDAI
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EDQGGETVRGEIRARFGERVTEIVDGCTETDITPKPPWAERKKAYIARLANASPSVRLVS
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHH
LADKLHNVRSILKDYRTQGDVLWNIFKGGKEGTLWYYRSLVTAFHQAGSTPMVVELDRLV
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHH
TELEGLVEIDMAPTVG
HHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]