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The map label for this gene is ntcA [H]

Identifier: 113475682

GI number: 113475682

Start: 3150496

End: 3151161

Strand: Reverse

Name: ntcA [H]

Synonym: Tery_2023

Alternate gene names: 113475682

Gene position: 3151161-3150496 (Counterclockwise)

Preceding gene: 113475684

Following gene: 113475681

Centisome position: 40.66

GC content: 39.79

Gene sequence:

>666_bases
GTGACGCATGATAGACCGCTAGCATCTGTTTTCCGTAATATATCTGGTGGCTCATATCCACCAGTGATAGAAACTTTTGA
GCGGAGCAAGACAATATTTTTTCCAGGAGATCCTGCCGAGCGTGTCTACGTCTTAATAAAAGGCGCAGTAAAGCTATCTA
GGGTTTATGAAGCAGGAGACGAAATTACAGTAGCCTTACTTAGGGAGAATAGTGTATTTGGTGTGCTATCTCTAATAACA
GGCCATAAGTCAGATCGTTTCTACCATGCAGTGGCATTTACTCCAGTAGAGTTAATATCAGTGCCAATTGACCAAGTAGA
AAAAGCTCTAAGAGAAGATCCAGAACTGTCAGTTATTCTATTGCGTGGACTATCATCCCGAATTTTACAGACAGAAATGA
TGATTGAAACCTTGGCACACCGAGATATGGGTTCTAGACTGGTTAGCTTTTTGTTAATCCTTTGTCGAGATTTTGGAGTT
CCTAGAGATGATGGGATTATGATTGACTTGAAATTATCTCACCAAGCAATTGCAGAAGCAATTGGGTCAACAAGGGTGAC
TGTTACTCGTCTACTTGGAGACCTCAGGCAAGATGAAATGATATCTATTCATAAAAAGAAAATTACAGTACATAACCCAG
TAGCTTTAAGTCAGCAATTTACTTAA

Upstream 100 bases:

>100_bases
TGCTGCCGACAACTTTGGTAGAAACAGGAAGTCAAAATTTAAATGTCATAACCAGTGAAGTTTTAGATCAGTTTTATGAA
GCAGTGTAAGGAAATTGCTC

Downstream 100 bases:

>100_bases
TTAAAAAAATAAAATAAATACTTGTAAAAATATTAATCATCCAACTTTTTATAGTGCTGACATCTTGCCAGAAATGGCTG
TAACTTACTTTTGATTAAAT

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTHDRPLASVFRNISGGSYPPVIETFERSKTIFFPGDPAERVYVLIKGAVKLSRVYEAGDEITVALLRENSVFGVLSLIT
GHKSDRFYHAVAFTPVELISVPIDQVEKALREDPELSVILLRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGV
PRDDGIMIDLKLSHQAIAEAIGSTRVTVTRLLGDLRQDEMISIHKKKITVHNPVALSQQFT

Sequences:

>Translated_221_residues
MTHDRPLASVFRNISGGSYPPVIETFERSKTIFFPGDPAERVYVLIKGAVKLSRVYEAGDEITVALLRENSVFGVLSLIT
GHKSDRFYHAVAFTPVELISVPIDQVEKALREDPELSVILLRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGV
PRDDGIMIDLKLSHQAIAEAIGSTRVTVTRLLGDLRQDEMISIHKKKITVHNPVALSQQFT
>Mature_220_residues
THDRPLASVFRNISGGSYPPVIETFERSKTIFFPGDPAERVYVLIKGAVKLSRVYEAGDEITVALLRENSVFGVLSLITG
HKSDRFYHAVAFTPVELISVPIDQVEKALREDPELSVILLRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGVP
RDDGIMIDLKLSHQAIAEAIGSTRVTVTRLLGDLRQDEMISIHKKKITVHNPVALSQQFT

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=196, Percent_Identity=25.5102040816327, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789756, Length=191, Percent_Identity=26.1780104712042, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24697; Mature: 24566

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDRPLASVFRNISGGSYPPVIETFERSKTIFFPGDPAERVYVLIKGAVKLSRVYEAGD
CCCCCHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCHHEEEEEEHHHHHHHHHHHCCC
EITVALLRENSVFGVLSLITGHKSDRFYHAVAFTPVELISVPIDQVEKALREDPELSVIL
CEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECHHHHHHCCHHHHHHHHHCCCCEEEEE
LRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGVPRDDGIMIDLKLSHQAIAEA
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
IGSTRVTVTRLLGDLRQDEMISIHKKKITVHNPVALSQQFT
HCCCHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCCCCCC
>Mature Secondary Structure 
THDRPLASVFRNISGGSYPPVIETFERSKTIFFPGDPAERVYVLIKGAVKLSRVYEAGD
CCCCHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCHHEEEEEEHHHHHHHHHHHCCC
EITVALLRENSVFGVLSLITGHKSDRFYHAVAFTPVELISVPIDQVEKALREDPELSVIL
CEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECHHHHHHCCHHHHHHHHHCCCCEEEEE
LRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGVPRDDGIMIDLKLSHQAIAEA
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
IGSTRVTVTRLLGDLRQDEMISIHKKKITVHNPVALSQQFT
HCCCHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]