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The map label for this gene is dnaE-N [H]

Identifier: 113475572

GI number: 113475572

Start: 2939721

End: 2941274

Strand: Reverse

Name: dnaE-N [H]

Synonym: Tery_1905

Alternate gene names: 113475572

Gene position: 2941274-2939721 (Counterclockwise)

Preceding gene: 113475575

Following gene: 113475570

Centisome position: 37.95

GC content: 31.72

Gene sequence:

>1554_bases
GTGGTAAAAATTGTCTCTCGCAAACTTGCCAAAACAGAAAATGTTTATGACATTGGTGTAACTAAAGACCATAATTTTGT
ATTAGCAAACGGGTTAATTGCTTCCAACTGTTTTAATAAATCTCATTCCACAGCTTACGCTTATGTAGCTTATCAAACTG
CTTATTTAAAAGCTAATTATTCACTCGAATATATGGCAGCTTTACTAACCGCAAATAGTGGCGACCAAGATAAAGTACAA
AAATATCTTGCTAATTGTCAGAAATTTAATATCGAAGTCGAACCACCAAATATTAATCAGTCAGAAGTAGACTTTACTCC
TTTGCCAAAAGTAGCCACAAAAGAAATAAAAGATAAAATTTTAGTAGGATTATCCGCTGTAAAAAATGTAGGAGAAGGAG
CAATAGAAGCAATTTTAAAAGCCAGAAAAGAAGGAGGAGAATTTAAGTCTTTAGCAGATTTATGTGACCGCGTTAATCTT
AATGCCCTTAATAGTCGTACTTTAGAATCTTTAATAAAATGTGGAGCCTTCGATAAAATTGAAGCAAACCGTCACCAATT
AATTAAAGACTTAGAAGGTGTAATGAAATGGGCACAAGATCGAAATAAAGACCGCAATATAGGACAATTAAGTTTATTTG
ATAATCATATCCCTGGAGCAAAAAATTCAGCTTTTGATTCTGTTCCTAAATCTTCCCCAGTTTCAGATTTTCCAGCCAAA
GAAAAATTGGAATATGAAAAAGAAATTCTAGGATTTTATGTATCTGATCATCCCCTTAAATATGCACAAGAACAAAATCA
AATTCAAGATGCTATTTCTATCGCTCAAATGAAAGAAAAAAAATCTAATTCCAAAATAAAATTAGTAGTAATGTTAGCTG
GAATTAAACCTCATGTTACAAAAAAAGGCGACCCTATGGCATTTATTCAAGTAGAAGATATGACCGGGCAACTAGATGCC
GTAGTTTTTCCAAAAGTATATCAAGAAATTAAAGAATTACTAACAGAAAATTCTCTTTTAGTTTTGAAAGGAAAAGTTGA
TAATAAAGATGACGAAAGTCAATTATTAGTAGAGAGTGCAGAAACAATAGAAAAGGTAATGGCAGATGCAGTTAAACAAG
AGGAAAAACCAGTGACAGAATTAGATAATTATCAAAATGAGTCAAGAGATGAAGAGATAAAAAAATATCAAGATATTGGA
AAAGCAGAAAAACCAGTAGAGCAATTAGAGACTCAAAGAAATCTTAGAAAAACTGAATTATCAAAAACTGAAAATAGTTC
TGCAAAACAAAGACAAATAATTATCTTGGAACTGTCAGTAGATAAGATTCCTGAAGATAATTCTTTGTTTGAACAAATTC
AAGCTATTTTGAAAGAACAATCTTCTGGAGATGAGGAAAAAGCAAAGGTAGCAGTTGGAACATTAATATTTGATGATCAA
CGACAACAGCTAGTACAATTCGGTCGTGAATTTTGGGTAGAAGATGGGCAGACAACTATTAATCGTTTTATAGGTATAGG
AATTGAAGCCAAGATTATTCCTTTTCCTTATTAA

Upstream 100 bases:

>100_bases
CTAAAACCTCAAAAAATTGACACCTAAAACTTAAAACCTAAAACCTCAAACCTAAAACCTAAAACCTATTCTATTTTTGT
CTAACAAAGGAGTTATTCTC

Downstream 100 bases:

>100_bases
TATTCATCATATATAAGATTAGAATCAGGCTTTCCCATCAAATATTTCCATCCATCCTGACCCTTAGTTTTTATTTTATG
AAGCTCAAAAAAAATAGCGT

Product: DNA polymerase III subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MVKIVSRKLAKTENVYDIGVTKDHNFVLANGLIASNCFNKSHSTAYAYVAYQTAYLKANYSLEYMAALLTANSGDQDKVQ
KYLANCQKFNIEVEPPNINQSEVDFTPLPKVATKEIKDKILVGLSAVKNVGEGAIEAILKARKEGGEFKSLADLCDRVNL
NALNSRTLESLIKCGAFDKIEANRHQLIKDLEGVMKWAQDRNKDRNIGQLSLFDNHIPGAKNSAFDSVPKSSPVSDFPAK
EKLEYEKEILGFYVSDHPLKYAQEQNQIQDAISIAQMKEKKSNSKIKLVVMLAGIKPHVTKKGDPMAFIQVEDMTGQLDA
VVFPKVYQEIKELLTENSLLVLKGKVDNKDDESQLLVESAETIEKVMADAVKQEEKPVTELDNYQNESRDEEIKKYQDIG
KAEKPVEQLETQRNLRKTELSKTENSSAKQRQIIILELSVDKIPEDNSLFEQIQAILKEQSSGDEEKAKVAVGTLIFDDQ
RQQLVQFGREFWVEDGQTTINRFIGIGIEAKIIPFPY

Sequences:

>Translated_517_residues
MVKIVSRKLAKTENVYDIGVTKDHNFVLANGLIASNCFNKSHSTAYAYVAYQTAYLKANYSLEYMAALLTANSGDQDKVQ
KYLANCQKFNIEVEPPNINQSEVDFTPLPKVATKEIKDKILVGLSAVKNVGEGAIEAILKARKEGGEFKSLADLCDRVNL
NALNSRTLESLIKCGAFDKIEANRHQLIKDLEGVMKWAQDRNKDRNIGQLSLFDNHIPGAKNSAFDSVPKSSPVSDFPAK
EKLEYEKEILGFYVSDHPLKYAQEQNQIQDAISIAQMKEKKSNSKIKLVVMLAGIKPHVTKKGDPMAFIQVEDMTGQLDA
VVFPKVYQEIKELLTENSLLVLKGKVDNKDDESQLLVESAETIEKVMADAVKQEEKPVTELDNYQNESRDEEIKKYQDIG
KAEKPVEQLETQRNLRKTELSKTENSSAKQRQIIILELSVDKIPEDNSLFEQIQAILKEQSSGDEEKAKVAVGTLIFDDQ
RQQLVQFGREFWVEDGQTTINRFIGIGIEAKIIPFPY
>Mature_517_residues
MVKIVSRKLAKTENVYDIGVTKDHNFVLANGLIASNCFNKSHSTAYAYVAYQTAYLKANYSLEYMAALLTANSGDQDKVQ
KYLANCQKFNIEVEPPNINQSEVDFTPLPKVATKEIKDKILVGLSAVKNVGEGAIEAILKARKEGGEFKSLADLCDRVNL
NALNSRTLESLIKCGAFDKIEANRHQLIKDLEGVMKWAQDRNKDRNIGQLSLFDNHIPGAKNSAFDSVPKSSPVSDFPAK
EKLEYEKEILGFYVSDHPLKYAQEQNQIQDAISIAQMKEKKSNSKIKLVVMLAGIKPHVTKKGDPMAFIQVEDMTGQLDA
VVFPKVYQEIKELLTENSLLVLKGKVDNKDDESQLLVESAETIEKVMADAVKQEEKPVTELDNYQNESRDEEIKKYQDIG
KAEKPVEQLETQRNLRKTELSKTENSSAKQRQIIILELSVDKIPEDNSLFEQIQAILKEQSSGDEEKAKVAVGTLIFDDQ
RQQLVQFGREFWVEDGQTTINRFIGIGIEAKIIPFPY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [H]

COG id: COG0587

COG function: function code L; DNA polymerase III, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-C family. DnaE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786381, Length=336, Percent_Identity=31.8452380952381, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR003587
- InterPro:   IPR006141
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR004805 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 58158; Mature: 58158

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50818 INTEIN_C_TER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIVSRKLAKTENVYDIGVTKDHNFVLANGLIASNCFNKSHSTAYAYVAYQTAYLKANY
CCCHHHHHHHHCCCEEEECCCCCCCEEEECCCHHHHHCCCCCCCEEEEEEEEEEEEEECC
SLEYMAALLTANSGDQDKVQKYLANCQKFNIEVEPPNINQSEVDFTPLPKVATKEIKDKI
CHHHHHHHHHCCCCCHHHHHHHHHCHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
LVGLSAVKNVGEGAIEAILKARKEGGEFKSLADLCDRVNLNALNSRTLESLIKCGAFDKI
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
EANRHQLIKDLEGVMKWAQDRNKDRNIGQLSLFDNHIPGAKNSAFDSVPKSSPVSDFPAK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCH
EKLEYEKEILGFYVSDHPLKYAQEQNQIQDAISIAQMKEKKSNSKIKLVVMLAGIKPHVT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
KKGDPMAFIQVEDMTGQLDAVVFPKVYQEIKELLTENSLLVLKGKVDNKDDESQLLVESA
CCCCCEEEEEEECCCCCCHHEECHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
ETIEKVMADAVKQEEKPVTELDNYQNESRDEEIKKYQDIGKAEKPVEQLETQRNLRKTEL
HHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SKTENSSAKQRQIIILELSVDKIPEDNSLFEQIQAILKEQSSGDEEKAKVAVGTLIFDDQ
HHCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHEEEEHEEECHH
RQQLVQFGREFWVEDGQTTINRFIGIGIEAKIIPFPY
HHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure
MVKIVSRKLAKTENVYDIGVTKDHNFVLANGLIASNCFNKSHSTAYAYVAYQTAYLKANY
CCCHHHHHHHHCCCEEEECCCCCCCEEEECCCHHHHHCCCCCCCEEEEEEEEEEEEEECC
SLEYMAALLTANSGDQDKVQKYLANCQKFNIEVEPPNINQSEVDFTPLPKVATKEIKDKI
CHHHHHHHHHCCCCCHHHHHHHHHCHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
LVGLSAVKNVGEGAIEAILKARKEGGEFKSLADLCDRVNLNALNSRTLESLIKCGAFDKI
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
EANRHQLIKDLEGVMKWAQDRNKDRNIGQLSLFDNHIPGAKNSAFDSVPKSSPVSDFPAK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCH
EKLEYEKEILGFYVSDHPLKYAQEQNQIQDAISIAQMKEKKSNSKIKLVVMLAGIKPHVT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
KKGDPMAFIQVEDMTGQLDAVVFPKVYQEIKELLTENSLLVLKGKVDNKDDESQLLVESA
CCCCCEEEEEEECCCCCCHHEECHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
ETIEKVMADAVKQEEKPVTELDNYQNESRDEEIKKYQDIGKAEKPVEQLETQRNLRKTEL
HHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SKTENSSAKQRQIIILELSVDKIPEDNSLFEQIQAILKEQSSGDEEKAKVAVGTLIFDDQ
HHCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHEEEEHEEECHH
RQQLVQFGREFWVEDGQTTINRFIGIGIEAKIIPFPY
HHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 9689062; 10734038; 11170467 [H]