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The map label for this gene is amtB [C]

Identifier: 113475459

GI number: 113475459

Start: 2730734

End: 2732224

Strand: Reverse

Name: amtB [C]

Synonym: Tery_1788

Alternate gene names: 113475459

Gene position: 2732224-2730734 (Counterclockwise)

Preceding gene: 113475460

Following gene: 113475450

Centisome position: 35.25

GC content: 37.83

Gene sequence:

>1491_bases
ATGTTAAGAAAATTGATGGATAAAAACTGGTTCTCACGTTTTAGGTATTTCTGTTTGTTTTTGGGAACTATCGTGGTTTT
GATAGGTTGCCAAGTAGCTTTAGCTAATGGTGGTAGTGATGATTTTGTGAAAGTAGATAATATCTGGCTATTGTTAGCTG
GGGCTCTGGTATTTTTTATGAATGCTGGTTTTGCTCTGTTGGAAGCAGGTTTTTGTCGCAGCGATAATTCTGTTAATGTC
TTGGCAAAAAACTTAATCGTTTTTTGTGTGGCGGCGATCGCCTTTTGGATGTTTGGTTTTGGCTTGATGTTTGGGGATAG
CTCATATCATCTTCCTTGTAGGGGAAACTTCGAGCAAAAGAATGAAAAAGTACAGAGTTATCACGGACATTTTTCTCCAA
CAATGGAATATAATGAGTTCGAGCAAAAGTCACAATTTATTCTACCGCCAAAAAATGATACTCCCCTAGGTTATCCTAGC
AAAGGATTTTCTTGTTTGCAGCGTCAGTTTCCTAAACGTTCTATCGCCTCTATATTTTTCTTTCAATTAGCATTTGCTGG
CACTGCAGCTACTATTGTTTCTAGAGCAGTTGCAGAAAAAATGAAACTTTGGGTATTTTTCTGCTTTAGTTTTATTTTGG
TAGGATTTTTATACCCTATTGTCGGTCATTGGGTATGGTCAGAATATGGTTGGCTCAAGCAAGGACTGAACTTTATAGAT
TTTGCCGGATCTACTGTTGTTCATACTGTGGGAGGAACTGCTGCTTTAGCAGTTGCTATATTACTTAAGCCAAGATGGTC
TTGGCTTGGATATGATCCCTGGGAAGATTTAGAAACGGATGAAGGTTCGGAAGCACTTTCTCCTCAGGGTCAAAAGGAAT
CCTATGATCAAAGAAATTTGACGTCTGCTACCCTCGGATGTCTGATACTTTGGTTAGGTTGGTTAGGCTTTAATGGTGGT
TCAACAACCGAGCTCAAAGAAGTAGGTCATATTATCATAACTACTATTATGTCTGCAGCAACTGGCGGGATTATTGCTAT
ATTACTTAGCCCATTATTTTCTCAAAGATCTAGCAACAACTCAGAGATTGGAAAACTAGATCTGTCATCACTAATTAATG
GTATATTAGGAGGCTTAGTTGGGATAACCGCTTCTGCTGCTTATGTAGATATTCCTATTGCCATTTTTATAGGTGCTGTT
AGTGGAGTGGTTGTCGTTTTAACTAGGAAACTTTTAAAGTGGATAAAACTTGAGGATCCATTAGGGGCTATTCCTGTTCA
TCTCTTTTGCGGATGCTGGGGTACTATTGCTGTATCTTTTAGCCTCAGAGGCACTATCGTTTATGAGAAAACTTATAATT
TCTTGGAGCGATTAGAATGGCAATTTTCAGGATGGGGCGTGGTTTTTATCACTACTTTTTTAGTTAGTTGTATAATCTGG
ATATTTATTGGGCTTACCTTATACTTATTTGAAAAAATACTTAGAAAATAA

Upstream 100 bases:

>100_bases
GGGTAATATTATTTTATGAGAATTTGATCAATCTCATGCTGTTTTCTCCAGCTGCTTGTTAAGGTATAATACTAACTATT
AATTATTTGACGGAGGTTTG

Downstream 100 bases:

>100_bases
AGAATTTTGTCTCAAGAAAGAAACTTATTGAAGAAAATAGCTAGAGTATTTAGGCAAGGAATTAAAGTAGTACCTCAAGA
AGAGTTCCAAGGTAGCGATA

Product: Rh-like protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MLRKLMDKNWFSRFRYFCLFLGTIVVLIGCQVALANGGSDDFVKVDNIWLLLAGALVFFMNAGFALLEAGFCRSDNSVNV
LAKNLIVFCVAAIAFWMFGFGLMFGDSSYHLPCRGNFEQKNEKVQSYHGHFSPTMEYNEFEQKSQFILPPKNDTPLGYPS
KGFSCLQRQFPKRSIASIFFFQLAFAGTAATIVSRAVAEKMKLWVFFCFSFILVGFLYPIVGHWVWSEYGWLKQGLNFID
FAGSTVVHTVGGTAALAVAILLKPRWSWLGYDPWEDLETDEGSEALSPQGQKESYDQRNLTSATLGCLILWLGWLGFNGG
STTELKEVGHIIITTIMSAATGGIIAILLSPLFSQRSSNNSEIGKLDLSSLINGILGGLVGITASAAYVDIPIAIFIGAV
SGVVVVLTRKLLKWIKLEDPLGAIPVHLFCGCWGTIAVSFSLRGTIVYEKTYNFLERLEWQFSGWGVVFITTFLVSCIIW
IFIGLTLYLFEKILRK

Sequences:

>Translated_496_residues
MLRKLMDKNWFSRFRYFCLFLGTIVVLIGCQVALANGGSDDFVKVDNIWLLLAGALVFFMNAGFALLEAGFCRSDNSVNV
LAKNLIVFCVAAIAFWMFGFGLMFGDSSYHLPCRGNFEQKNEKVQSYHGHFSPTMEYNEFEQKSQFILPPKNDTPLGYPS
KGFSCLQRQFPKRSIASIFFFQLAFAGTAATIVSRAVAEKMKLWVFFCFSFILVGFLYPIVGHWVWSEYGWLKQGLNFID
FAGSTVVHTVGGTAALAVAILLKPRWSWLGYDPWEDLETDEGSEALSPQGQKESYDQRNLTSATLGCLILWLGWLGFNGG
STTELKEVGHIIITTIMSAATGGIIAILLSPLFSQRSSNNSEIGKLDLSSLINGILGGLVGITASAAYVDIPIAIFIGAV
SGVVVVLTRKLLKWIKLEDPLGAIPVHLFCGCWGTIAVSFSLRGTIVYEKTYNFLERLEWQFSGWGVVFITTFLVSCIIW
IFIGLTLYLFEKILRK
>Mature_496_residues
MLRKLMDKNWFSRFRYFCLFLGTIVVLIGCQVALANGGSDDFVKVDNIWLLLAGALVFFMNAGFALLEAGFCRSDNSVNV
LAKNLIVFCVAAIAFWMFGFGLMFGDSSYHLPCRGNFEQKNEKVQSYHGHFSPTMEYNEFEQKSQFILPPKNDTPLGYPS
KGFSCLQRQFPKRSIASIFFFQLAFAGTAATIVSRAVAEKMKLWVFFCFSFILVGFLYPIVGHWVWSEYGWLKQGLNFID
FAGSTVVHTVGGTAALAVAILLKPRWSWLGYDPWEDLETDEGSEALSPQGQKESYDQRNLTSATLGCLILWLGWLGFNGG
STTELKEVGHIIITTIMSAATGGIIAILLSPLFSQRSSNNSEIGKLDLSSLINGILGGLVGITASAAYVDIPIAIFIGAV
SGVVVVLTRKLLKWIKLEDPLGAIPVHLFCGCWGTIAVSFSLRGTIVYEKTYNFLERLEWQFSGWGVVFITTFLVSCIIW
IFIGLTLYLFEKILRK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=396, Percent_Identity=25, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI133931100, Length=414, Percent_Identity=28.5024154589372, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI32564042, Length=405, Percent_Identity=30.3703703703704, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17550180, Length=420, Percent_Identity=30.4761904761905, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17550182, Length=424, Percent_Identity=30.188679245283, Blast_Score=157, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324187, Length=391, Percent_Identity=26.5984654731458, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325396, Length=389, Percent_Identity=23.6503856041131, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321559, Length=389, Percent_Identity=24.9357326478149, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI161078314, Length=465, Percent_Identity=31.8279569892473, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 55154; Mature: 55154

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]