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The map label for this gene is ndhF [H]

Identifier: 113474511

GI number: 113474511

Start: 1076583

End: 1078646

Strand: Reverse

Name: ndhF [H]

Synonym: Tery_0660

Alternate gene names: 113474511

Gene position: 1078646-1076583 (Counterclockwise)

Preceding gene: 113474515

Following gene: 113474510

Centisome position: 13.92

GC content: 43.07

Gene sequence:

>2064_bases
ATGGAAACACTTTATCAATATGCCTGGCTAATTCCAGTACTGCCGCTACTGGGAGCAACCTTAGTTGGCTTTGGCCTAAT
TTCCTATAGCCGAGCCACAAGTAATTTACGACAGGGAGCCGCAATATTTATTGTCTCTCTGTTGGGAACTGCAATGGTTT
TATCCTTTGCATTGCTCTGGAGCCAAATAAATGGCCATGAAATCTATACCCAAATGTTTGAGTGGGCAGCAGCAGGTGAC
TTCAAGCTCAGTATGGGCTACACCATCGATCACTTGACTGCCCTCATGTTAGTCATAGTCACTACCGTTGCCTTCTTGGT
AATGGTCTACACAGATGGCTACATGGCTCACGATCCTGGATATGTACGCTTTTACGCTTACTTGAGCTTATTTAGCTCCT
CAATGTTAGGTCTAGTAATTTCTCCAAACCTAGTCCAAATTTATATTTTCTGGGAACTTGTTGGTGTATCCTCATACCTA
CTAATTGGTTTCTGGTACGACCGTAAAGCAGCAGCAGAAGCCTGTCAAAAAGCTTTTGTTGTCAACCGTGTCGGTGACTT
TGGTTTACTCCTGGGAATGCTAGGAATTTACTGGGCCACTGGTACCTTTGAATTTGAGGCTATGGGTCTTCGCTTGGAGC
AACTAGTAGAAACAGGAGCCCTAAGCATTGGTTTAGCTACCCTTTTCGGTGTCTTAGTTTTCCTAGGACCTGTAGCTAAG
TCAGCCCAATTCCCACTGCATGTTTGGTTGCCCGACGCAATGGAAGGCCCGACTCCAATTTCTGCCCTGATCCACGCAGC
AACGATGGTAGCTGCTGGAGTATTCCTAATTGCCCGGATGTATCCAGTATTTGAACATCTGCCAGTGGTAATGGATATTA
TCGCTTGGACGGGAGCCTTTACAGCTTTTCTGGGGGCAACGATCGCTATTACTCAAAATGATATTAAAAAAGGTCTGGCC
TATTCGACTATTTCCCAGTTGGGCTACATGGTAATGGCAATGGGTGTGGGTGCTTATAGCGCTGGACTATTTCACCTAAT
GACCCATGCTTATTTCAAAGCTATGCTGTTCCTCTGTTCTGGTTCTGTAATTCATGGAATGGAGGCAGTGGTTGGCCATA
ATCCAGTTTTGGCTCAAGATATGCGGTTAATGGGTGGACTCAGGAAATATATGCCTATTACCTCTACTTGCTTTTTAATT
GGTACTGTGGCAATTTGTGGTATCCCTCCCTTTGCAGGTTTTTGGTCAAAAGATGAAATTTTAGGCAGTGCCTTTGGAGC
AAACCCAGCTTTATGGTTCATTGGTTGGGCAACTGCTGGCATGACTGCTTTTTATATGTTCCGTATGTATTTCTCTACTT
TTGAAGGAGAATTTAGGGGTAATAACACAAAAATTAAGGATCAATTATTGCTGGCAAATGCTGCACCCCTGATGGAACTT
AGCCCTGGGGCTATAGCTCCCAAAGAGTTGGAAAATGGCCATGATAGCCATGACCACGACCATGATCATAGCCACTCTCC
CCATGAGTCTCCTGTGAGCATGACTCTACCATTAATGGTTTTGGCAATTCCTTCGATGGTTATTGGTTTATTGGGAACAC
CTTTTGCTAATTATTTTGAACAGTTTATTTATGCTCCTGGAGAGTCTTTGGCACAAGTCCTGGAGGAGTTGGCAGAGTTT
GAGCTTAATGAATTTTTGATTATGGCGGGTAACTCTGTTGGTATTGCTTTAATTGGTATTACCTTTGCTTCACTTATGTA
TTTGAGTCATAAAATTGACCCAGATGCGATCGCTAAAAAAATCCCATCTCTCTACAACTTTTCCCTCAACAAATGGTATC
TAGATAATATCAACGATTTTCTATTTGTTAAAGGTAGTCGTCGTCTAGCACGGCAAGTTTTGGAAGTGGACTACAAGATA
GTTGATGGAGCTGTTAACTTGACAGGCTTTATTACCCTATTGAGTGGGGAAGGTTTGAAGTATTTAGAAAATGGTCGAGC
TCAGTTTTATGCCTTAATTGTATTTGTGGCAGTTTTGGGCTTCGTTGTTTTCTCTGGTGCCTAG

Upstream 100 bases:

>100_bases
TAAAAAATCTGTCTTTGACATCAATAATCAAGTAAGTGTTATGTAAGAATGCTTATTAGTTTTGCCCACTTAGTAAAGAA
ATGTAAAGTAACCATAAGTT

Downstream 100 bases:

>100_bases
TTAATTATCATTGACTAGTAGTTTTCCTAGTGATATTTGGTGGAATACTTTTTTTGTTAATTTCCTGTTTATAGGGAACA
TTTCAAATTTAGTAGTGTCG

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
METLYQYAWLIPVLPLLGATLVGFGLISYSRATSNLRQGAAIFIVSLLGTAMVLSFALLWSQINGHEIYTQMFEWAAAGD
FKLSMGYTIDHLTALMLVIVTTVAFLVMVYTDGYMAHDPGYVRFYAYLSLFSSSMLGLVISPNLVQIYIFWELVGVSSYL
LIGFWYDRKAAAEACQKAFVVNRVGDFGLLLGMLGIYWATGTFEFEAMGLRLEQLVETGALSIGLATLFGVLVFLGPVAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLIARMYPVFEHLPVVMDIIAWTGAFTAFLGATIAITQNDIKKGLA
YSTISQLGYMVMAMGVGAYSAGLFHLMTHAYFKAMLFLCSGSVIHGMEAVVGHNPVLAQDMRLMGGLRKYMPITSTCFLI
GTVAICGIPPFAGFWSKDEILGSAFGANPALWFIGWATAGMTAFYMFRMYFSTFEGEFRGNNTKIKDQLLLANAAPLMEL
SPGAIAPKELENGHDSHDHDHDHSHSPHESPVSMTLPLMVLAIPSMVIGLLGTPFANYFEQFIYAPGESLAQVLEELAEF
ELNEFLIMAGNSVGIALIGITFASLMYLSHKIDPDAIAKKIPSLYNFSLNKWYLDNINDFLFVKGSRRLARQVLEVDYKI
VDGAVNLTGFITLLSGEGLKYLENGRAQFYALIVFVAVLGFVVFSGA

Sequences:

>Translated_687_residues
METLYQYAWLIPVLPLLGATLVGFGLISYSRATSNLRQGAAIFIVSLLGTAMVLSFALLWSQINGHEIYTQMFEWAAAGD
FKLSMGYTIDHLTALMLVIVTTVAFLVMVYTDGYMAHDPGYVRFYAYLSLFSSSMLGLVISPNLVQIYIFWELVGVSSYL
LIGFWYDRKAAAEACQKAFVVNRVGDFGLLLGMLGIYWATGTFEFEAMGLRLEQLVETGALSIGLATLFGVLVFLGPVAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLIARMYPVFEHLPVVMDIIAWTGAFTAFLGATIAITQNDIKKGLA
YSTISQLGYMVMAMGVGAYSAGLFHLMTHAYFKAMLFLCSGSVIHGMEAVVGHNPVLAQDMRLMGGLRKYMPITSTCFLI
GTVAICGIPPFAGFWSKDEILGSAFGANPALWFIGWATAGMTAFYMFRMYFSTFEGEFRGNNTKIKDQLLLANAAPLMEL
SPGAIAPKELENGHDSHDHDHDHSHSPHESPVSMTLPLMVLAIPSMVIGLLGTPFANYFEQFIYAPGESLAQVLEELAEF
ELNEFLIMAGNSVGIALIGITFASLMYLSHKIDPDAIAKKIPSLYNFSLNKWYLDNINDFLFVKGSRRLARQVLEVDYKI
VDGAVNLTGFITLLSGEGLKYLENGRAQFYALIVFVAVLGFVVFSGA
>Mature_687_residues
METLYQYAWLIPVLPLLGATLVGFGLISYSRATSNLRQGAAIFIVSLLGTAMVLSFALLWSQINGHEIYTQMFEWAAAGD
FKLSMGYTIDHLTALMLVIVTTVAFLVMVYTDGYMAHDPGYVRFYAYLSLFSSSMLGLVISPNLVQIYIFWELVGVSSYL
LIGFWYDRKAAAEACQKAFVVNRVGDFGLLLGMLGIYWATGTFEFEAMGLRLEQLVETGALSIGLATLFGVLVFLGPVAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLIARMYPVFEHLPVVMDIIAWTGAFTAFLGATIAITQNDIKKGLA
YSTISQLGYMVMAMGVGAYSAGLFHLMTHAYFKAMLFLCSGSVIHGMEAVVGHNPVLAQDMRLMGGLRKYMPITSTCFLI
GTVAICGIPPFAGFWSKDEILGSAFGANPALWFIGWATAGMTAFYMFRMYFSTFEGEFRGNNTKIKDQLLLANAAPLMEL
SPGAIAPKELENGHDSHDHDHDHSHSPHESPVSMTLPLMVLAIPSMVIGLLGTPFANYFEQFIYAPGESLAQVLEELAEF
ELNEFLIMAGNSVGIALIGITFASLMYLSHKIDPDAIAKKIPSLYNFSLNKWYLDNINDFLFVKGSRRLARQVLEVDYKI
VDGAVNLTGFITLLSGEGLKYLENGRAQFYALIVFVAVLGFVVFSGA

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=422, Percent_Identity=37.2037914691943, Blast_Score=277, Evalue=2e-74,
Organism=Escherichia coli, GI1788614, Length=655, Percent_Identity=36.9465648854962, Blast_Score=335, Evalue=5e-93,
Organism=Escherichia coli, GI1788829, Length=372, Percent_Identity=32.5268817204301, Blast_Score=167, Evalue=3e-42,
Organism=Escherichia coli, GI1788827, Length=472, Percent_Identity=29.6610169491525, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788831, Length=376, Percent_Identity=30.0531914893617, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788613, Length=373, Percent_Identity=26.8096514745308, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI145693160, Length=301, Percent_Identity=27.5747508305648, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI2367154, Length=417, Percent_Identity=23.7410071942446, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 75214; Mature: 75214

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLYQYAWLIPVLPLLGATLVGFGLISYSRATSNLRQGAAIFIVSLLGTAMVLSFALLW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
SQINGHEIYTQMFEWAAAGDFKLSMGYTIDHLTALMLVIVTTVAFLVMVYTDGYMAHDPG
HHCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
YVRFYAYLSLFSSSMLGLVISPNLVQIYIFWELVGVSSYLLIGFWYDRKAAAEACQKAFV
HHHHHHHHHHHHHHHHCEEECCCEEEEEHHHHHHHHHHEEHEEEECCHHHHHHHHHHHHH
VNRVGDFGLLLGMLGIYWATGTFEFEAMGLRLEQLVETGALSIGLATLFGVLVFLGPVAK
HHCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLIARMYPVFEHLPVVMDIIAWTGAF
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAFLGATIAITQNDIKKGLAYSTISQLGYMVMAMGVGAYSAGLFHLMTHAYFKAMLFLCS
HHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GSVIHGMEAVVGHNPVLAQDMRLMGGLRKYMPITSTCFLIGTVAICGIPPFAGFWSKDEI
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LGSAFGANPALWFIGWATAGMTAFYMFRMYFSTFEGEFRGNNTKIKDQLLLANAAPLMEL
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCC
SPGAIAPKELENGHDSHDHDHDHSHSPHESPVSMTLPLMVLAIPSMVIGLLGTPFANYFE
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QFIYAPGESLAQVLEELAEFELNEFLIMAGNSVGIALIGITFASLMYLSHKIDPDAIAKK
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
IPSLYNFSLNKWYLDNINDFLFVKGSRRLARQVLEVDYKIVDGAVNLTGFITLLSGEGLK
CCHHHCCCCCCEECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHH
YLENGRAQFYALIVFVAVLGFVVFSGA
HHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METLYQYAWLIPVLPLLGATLVGFGLISYSRATSNLRQGAAIFIVSLLGTAMVLSFALLW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
SQINGHEIYTQMFEWAAAGDFKLSMGYTIDHLTALMLVIVTTVAFLVMVYTDGYMAHDPG
HHCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
YVRFYAYLSLFSSSMLGLVISPNLVQIYIFWELVGVSSYLLIGFWYDRKAAAEACQKAFV
HHHHHHHHHHHHHHHHCEEECCCEEEEEHHHHHHHHHHEEHEEEECCHHHHHHHHHHHHH
VNRVGDFGLLLGMLGIYWATGTFEFEAMGLRLEQLVETGALSIGLATLFGVLVFLGPVAK
HHCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLIARMYPVFEHLPVVMDIIAWTGAF
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAFLGATIAITQNDIKKGLAYSTISQLGYMVMAMGVGAYSAGLFHLMTHAYFKAMLFLCS
HHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GSVIHGMEAVVGHNPVLAQDMRLMGGLRKYMPITSTCFLIGTVAICGIPPFAGFWSKDEI
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LGSAFGANPALWFIGWATAGMTAFYMFRMYFSTFEGEFRGNNTKIKDQLLLANAAPLMEL
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCC
SPGAIAPKELENGHDSHDHDHDHSHSPHESPVSMTLPLMVLAIPSMVIGLLGTPFANYFE
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
QFIYAPGESLAQVLEELAEFELNEFLIMAGNSVGIALIGITFASLMYLSHKIDPDAIAKK
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
IPSLYNFSLNKWYLDNINDFLFVKGSRRLARQVLEVDYKIVDGAVNLTGFITLLSGEGLK
CCHHHCCCCCCEECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHH
YLENGRAQFYALIVFVAVLGFVVFSGA
HHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]