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The map label for this gene is murF [H]

Identifier: 113474363

GI number: 113474363

Start: 793660

End: 795066

Strand: Reverse

Name: murF [H]

Synonym: Tery_0495

Alternate gene names: 113474363

Gene position: 795066-793660 (Counterclockwise)

Preceding gene: 113474367

Following gene: 113474353

Centisome position: 10.26

GC content: 33.19

Gene sequence:

>1407_bases
ATGGTTTGTTGCATGACTTTATTTCAACTGACCGAAATATTAACTACTACAACACTTACAGGTTTTCATACTCCAGAAAA
AACATTAATTACAAGCATAAATACAGATACTCGTAACTTACAGCCTGGTGAAGGATTTTTGGCATTACGAGGAAATAATT
TTGATGGCCATAATTTTGTTGACGAAGCAATCGAAAAGGGAGCTTCTTGTGTGATTGTTGAACAAGAATATACCTTAAAT
AGTGAGATTCAACAACTATCAAATAATATACCTATATTACAAGTAGAAAATACTTTAAAAGCTTACCAAAAAATTGCTCG
TTGGTGGCGAGATAGATTTGATATTCCTGTTATTTCGGTCACAGGTTCAGTAGGTAAAACAACTACTAAAGAATTAATTG
CTGAAGTTTTAGCAACTCAGGGTAAAGTCATGAAAACTGAAGCTAATTATAACAATGAAATAGGTGTAGCTAAAACTCTA
CTACAACTTTCTAATGAAGAGAATTATGCAGTAGTAGAAATGGCAATGCGGGGACCAGGAGAAATAGCAGAGTTAACACA
TATTGCTAACCCGACAATTGGTTTAATTACTAATGTCGGTACTGCTCATATTGGTCGTTTGGGTTCTGTTGAAGCCATTG
CTGAAGCTAAGTGTGAATTGTTAAGAGAAATGTCTGATAGTAGTATTGCTATTCTGAATTATGATAATCAACGGTTGATA
GAAACTGCGGCTAAAGTTTGGTCTGGAGAAACTTTTACTTATGGTTTAGAAGGTGGAAATTTACAAGGAAAATTAATTGA
TTCTCAAACGATAAATGTTGAGGGTGTGGATTTACCTTTACCTTTACCTGGTAGTCATAATGCTTCTAATTATTTGGCAG
CATTAGCAGTCTTAAAAGTGCTTCATGGAAAAAATATTCCCGCAAAAACTCTGTTTCAATCTTTGACTAATAACTTATTC
GTTCAATTACCAGGAAGTAGGGCAAGAAAATATGATTTAAAGAATGATATTGTAATTTTAGATGAAAGTTATAATGCTGG
ATTAGAGTCAATGATAGCTGCTCTCAATTTGTTAGCTGAAACTCCTGGTAAACGACATATTGCTGTATTGGGAACTATGA
AAGAATTGGGAAACAAATCAATTTTATTTCATCAACAAGTTGGTAATGTTGTCAAAAATTTGAATATTGATGGTTTATTT
ATTTTAGCTGATTTTGAGGAAGCAAAAGCAATGGCTACAGGTGCTGCTAGTTTGCAATTTATCAAGGTAGAAAATATCAC
TGCTGATAATGCTCATCATAATTTAGCCAAATATTTACAGGAGTTTTTACAGCCTGGTGATCGGGTTCTTTTTAAGGCTT
CTCATTCTGTAGAGTTAAATAAAGTAGTCGAACTTTTAATTAATTAG

Upstream 100 bases:

>100_bases
CTTGGTTAATCAGCTAATTCCCAGAAAATAAGTTTAGTTTAAGAACCACAAGTACTCTTCTATAATTATAATATATCTTT
TAGGAGAACTGCCAATAGCT

Downstream 100 bases:

>100_bases
TAATGCATTTATAGCATAGACATCTTTCCTGTTGCGGGTGTATCTTCAACTAATATGAAACACCCTGCAACATTAAATCT
TAAAAAGCATCAGAAGCCTA

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MVCCMTLFQLTEILTTTTLTGFHTPEKTLITSINTDTRNLQPGEGFLALRGNNFDGHNFVDEAIEKGASCVIVEQEYTLN
SEIQQLSNNIPILQVENTLKAYQKIARWWRDRFDIPVISVTGSVGKTTTKELIAEVLATQGKVMKTEANYNNEIGVAKTL
LQLSNEENYAVVEMAMRGPGEIAELTHIANPTIGLITNVGTAHIGRLGSVEAIAEAKCELLREMSDSSIAILNYDNQRLI
ETAAKVWSGETFTYGLEGGNLQGKLIDSQTINVEGVDLPLPLPGSHNASNYLAALAVLKVLHGKNIPAKTLFQSLTNNLF
VQLPGSRARKYDLKNDIVILDESYNAGLESMIAALNLLAETPGKRHIAVLGTMKELGNKSILFHQQVGNVVKNLNIDGLF
ILADFEEAKAMATGAASLQFIKVENITADNAHHNLAKYLQEFLQPGDRVLFKASHSVELNKVVELLIN

Sequences:

>Translated_468_residues
MVCCMTLFQLTEILTTTTLTGFHTPEKTLITSINTDTRNLQPGEGFLALRGNNFDGHNFVDEAIEKGASCVIVEQEYTLN
SEIQQLSNNIPILQVENTLKAYQKIARWWRDRFDIPVISVTGSVGKTTTKELIAEVLATQGKVMKTEANYNNEIGVAKTL
LQLSNEENYAVVEMAMRGPGEIAELTHIANPTIGLITNVGTAHIGRLGSVEAIAEAKCELLREMSDSSIAILNYDNQRLI
ETAAKVWSGETFTYGLEGGNLQGKLIDSQTINVEGVDLPLPLPGSHNASNYLAALAVLKVLHGKNIPAKTLFQSLTNNLF
VQLPGSRARKYDLKNDIVILDESYNAGLESMIAALNLLAETPGKRHIAVLGTMKELGNKSILFHQQVGNVVKNLNIDGLF
ILADFEEAKAMATGAASLQFIKVENITADNAHHNLAKYLQEFLQPGDRVLFKASHSVELNKVVELLIN
>Mature_468_residues
MVCCMTLFQLTEILTTTTLTGFHTPEKTLITSINTDTRNLQPGEGFLALRGNNFDGHNFVDEAIEKGASCVIVEQEYTLN
SEIQQLSNNIPILQVENTLKAYQKIARWWRDRFDIPVISVTGSVGKTTTKELIAEVLATQGKVMKTEANYNNEIGVAKTL
LQLSNEENYAVVEMAMRGPGEIAELTHIANPTIGLITNVGTAHIGRLGSVEAIAEAKCELLREMSDSSIAILNYDNQRLI
ETAAKVWSGETFTYGLEGGNLQGKLIDSQTINVEGVDLPLPLPGSHNASNYLAALAVLKVLHGKNIPAKTLFQSLTNNLF
VQLPGSRARKYDLKNDIVILDESYNAGLESMIAALNLLAETPGKRHIAVLGTMKELGNKSILFHQQVGNVVKNLNIDGLF
ILADFEEAKAMATGAASLQFIKVENITADNAHHNLAKYLQEFLQPGDRVLFKASHSVELNKVVELLIN

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=423, Percent_Identity=32.8605200945626, Blast_Score=188, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51225; Mature: 51225

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCCMTLFQLTEILTTTTLTGFHTPEKTLITSINTDTRNLQPGEGFLALRGNNFDGHNFV
CHHHHHHHHHHHHHHHHEECCCCCCCHHEEEEECCCCCCCCCCCCEEEEECCCCCCCHHH
DEAIEKGASCVIVEQEYTLNSEIQQLSNNIPILQVENTLKAYQKIARWWRDRFDIPVISV
HHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEE
TGSVGKTTTKELIAEVLATQGKVMKTEANYNNEIGVAKTLLQLSNEENYAVVEMAMRGPG
CCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCC
EIAELTHIANPTIGLITNVGTAHIGRLGSVEAIAEAKCELLREMSDSSIAILNYDNQRLI
HHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
ETAAKVWSGETFTYGLEGGNLQGKLIDSQTINVEGVDLPLPLPGSHNASNYLAALAVLKV
HHHHHHHCCCEEEEECCCCCEEEEEECCEEEEEECCEECCCCCCCCCHHHHHHHHHHHHH
LHGKNIPAKTLFQSLTNNLFVQLPGSRARKYDLKNDIVILDESYNAGLESMIAALNLLAE
HHCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHC
TPGKRHIAVLGTMKELGNKSILFHQQVGNVVKNLNIDGLFILADFEEAKAMATGAASLQF
CCCCCEEEEEEEHHHHCCCCEEEHHHHHHHHHCCCCCEEEEEEECHHHHHHHCCCCEEEE
IKVENITADNAHHNLAKYLQEFLQPGDRVLFKASHSVELNKVVELLIN
EEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MVCCMTLFQLTEILTTTTLTGFHTPEKTLITSINTDTRNLQPGEGFLALRGNNFDGHNFV
CHHHHHHHHHHHHHHHHEECCCCCCCHHEEEEECCCCCCCCCCCCEEEEECCCCCCCHHH
DEAIEKGASCVIVEQEYTLNSEIQQLSNNIPILQVENTLKAYQKIARWWRDRFDIPVISV
HHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEE
TGSVGKTTTKELIAEVLATQGKVMKTEANYNNEIGVAKTLLQLSNEENYAVVEMAMRGPG
CCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCC
EIAELTHIANPTIGLITNVGTAHIGRLGSVEAIAEAKCELLREMSDSSIAILNYDNQRLI
HHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
ETAAKVWSGETFTYGLEGGNLQGKLIDSQTINVEGVDLPLPLPGSHNASNYLAALAVLKV
HHHHHHHCCCEEEEECCCCCEEEEEECCEEEEEECCEECCCCCCCCCHHHHHHHHHHHHH
LHGKNIPAKTLFQSLTNNLFVQLPGSRARKYDLKNDIVILDESYNAGLESMIAALNLLAE
HHCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHC
TPGKRHIAVLGTMKELGNKSILFHQQVGNVVKNLNIDGLFILADFEEAKAMATGAASLQF
CCCCCEEEEEEEHHHHCCCCEEEHHHHHHHHHCCCCCEEEEEEECHHHHHHHCCCCEEEE
IKVENITADNAHHNLAKYLQEFLQPGDRVLFKASHSVELNKVVELLIN
EEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7894708; 8905231 [H]