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The map label for this gene is ybiK [C]

Identifier: 113474154

GI number: 113474154

Start: 401698

End: 402621

Strand: Direct

Name: ybiK [C]

Synonym: Tery_0258

Alternate gene names: 113474154

Gene position: 401698-402621 (Clockwise)

Preceding gene: 113474153

Following gene: 113474164

Centisome position: 5.18

GC content: 40.48

Gene sequence:

>924_bases
ATGCAGCCAAAACTTATTATTCATGGTGGTGCTGGTAGTTCCCTTGACGAAAAAGGAGGATTAGAAATTGTTCGGAAATC
TTTACATCGAATAGTAGATGAATTATATAACCTACTTCTAGGTGATACCGCTGCTCTAGATATAGTTATACGTGGTTGCC
AAATGTTAGAAGATGACCCAATTTTTAATGCTGGCACCGGTTCAGTGCTCCAATCTGATGGACAAATTCGGATGAGTGCT
TCGCTGATGGATGGTTTAAATCATCGGTTTAGTGGGGTGATCAATGTCTCCAGGATTAAAAATCCCATTGATTTAGCCAG
AGCATTGCAAGAATCCCCTGATCGCGTATTATCAGATAGTGGGGCGGCTGAATTTGTGCGGGAATTACAAATGCCAGTTT
ACAATCCTCTGACAGATAAACGATTAAATGAATGGCTTGAAGACCGCAAACAAAATTTTAATAGAAAAATGGCAAACGTG
GTTACAGTATCTCCAGGTACTGGCACTATTGGTGTAGTAGCTTTGGATAAAGAGGGTAGATTAGCTGCAGGTACGTCTAC
TGGTGGGAAAGGTTTTGAAAAAATTGGTCGGGTTAGTGACTCGGCGATGCCTGCGGGAAATTATGCTACTGATAGTGCTG
CAGTAAGTTGTACTGGTATTGGAGAAGATATTATTGATGAGTGTTTGGCTGCAAAAATTGTGATTAGGGTAACGGATGGT
TTTTCTATTAAGGCAGCTTTTGAGCGTTCATTTGCTGAGGCCCATGAACATAAAAGAGATTTGGGAGCTATCGGTATTGA
TGCTGGTGGTACAATTAACTGGGGAAAAACTTGCGATGTAATTTTAGCTGCTTTTCACGATGGGGAAAAAATTGGTGATA
GTTTGGAAGCTGACAAGGGAAAATCAACTTTTTCTGTTGAATAA

Upstream 100 bases:

>100_bases
AAAAACAGGTAGAACTTATTTATTGGTCAAGATTGACAAAAAAACGAGACTTACTTAATATTATATCTCAACTAAGTTAT
TCTGTAGTTAATACAAGAAA

Downstream 100 bases:

>100_bases
AGCCAGGGCTTACCCATCTAAACAAGGAGGTTTGTTCCCCTGGTTTTTTATTGTGATTCAAACCAATAAACTTCCTTGAT
ATATCCGTTTTCTGCATAAG

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MQPKLIIHGGAGSSLDEKGGLEIVRKSLHRIVDELYNLLLGDTAALDIVIRGCQMLEDDPIFNAGTGSVLQSDGQIRMSA
SLMDGLNHRFSGVINVSRIKNPIDLARALQESPDRVLSDSGAAEFVRELQMPVYNPLTDKRLNEWLEDRKQNFNRKMANV
VTVSPGTGTIGVVALDKEGRLAAGTSTGGKGFEKIGRVSDSAMPAGNYATDSAAVSCTGIGEDIIDECLAAKIVIRVTDG
FSIKAAFERSFAEAHEHKRDLGAIGIDAGGTINWGKTCDVILAAFHDGEKIGDSLEADKGKSTFSVE

Sequences:

>Translated_307_residues
MQPKLIIHGGAGSSLDEKGGLEIVRKSLHRIVDELYNLLLGDTAALDIVIRGCQMLEDDPIFNAGTGSVLQSDGQIRMSA
SLMDGLNHRFSGVINVSRIKNPIDLARALQESPDRVLSDSGAAEFVRELQMPVYNPLTDKRLNEWLEDRKQNFNRKMANV
VTVSPGTGTIGVVALDKEGRLAAGTSTGGKGFEKIGRVSDSAMPAGNYATDSAAVSCTGIGEDIIDECLAAKIVIRVTDG
FSIKAAFERSFAEAHEHKRDLGAIGIDAGGTINWGKTCDVILAAFHDGEKIGDSLEADKGKSTFSVE
>Mature_307_residues
MQPKLIIHGGAGSSLDEKGGLEIVRKSLHRIVDELYNLLLGDTAALDIVIRGCQMLEDDPIFNAGTGSVLQSDGQIRMSA
SLMDGLNHRFSGVINVSRIKNPIDLARALQESPDRVLSDSGAAEFVRELQMPVYNPLTDKRLNEWLEDRKQNFNRKMANV
VTVSPGTGTIGVVALDKEGRLAAGTSTGGKGFEKIGRVSDSAMPAGNYATDSAAVSCTGIGEDIIDECLAAKIVIRVTDG
FSIKAAFERSFAEAHEHKRDLGAIGIDAGGTINWGKTCDVILAAFHDGEKIGDSLEADKGKSTFSVE

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Probably performs the final step in the degradation of the reserve polymer cyanophycin (depolymerizes the building block L-beta-Asp-Arg). Also has L- asparagin

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=269, Percent_Identity=36.0594795539033, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI145275200, Length=269, Percent_Identity=36.0594795539033, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI98991769, Length=264, Percent_Identity=29.5454545454545, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787050, Length=270, Percent_Identity=36.2962962962963, Blast_Score=112, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18921183, Length=289, Percent_Identity=30.4498269896194, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357893, Length=263, Percent_Identity=29.6577946768061, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 32773; Mature: 32773

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPKLIIHGGAGSSLDEKGGLEIVRKSLHRIVDELYNLLLGDTAALDIVIRGCQMLEDDP
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
IFNAGTGSVLQSDGQIRMSASLMDGLNHRFSGVINVSRIKNPIDLARALQESPDRVLSDS
CCCCCCCHHEECCCCEEEHHHHHHHHHHHHHCEEEHHHCCCHHHHHHHHHHCHHHHHCCC
GAAEFVRELQMPVYNPLTDKRLNEWLEDRKQNFNRKMANVVTVSPGTGTIGVVALDKEGR
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCC
LAAGTSTGGKGFEKIGRVSDSAMPAGNYATDSAAVSCTGIGEDIIDECLAAKIVIRVTDG
EEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEEEEECC
FSIKAAFERSFAEAHEHKRDLGAIGIDAGGTINWGKTCDVILAAFHDGEKIGDSLEADKG
CEEHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCC
KSTFSVE
CCCCCCC
>Mature Secondary Structure
MQPKLIIHGGAGSSLDEKGGLEIVRKSLHRIVDELYNLLLGDTAALDIVIRGCQMLEDDP
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
IFNAGTGSVLQSDGQIRMSASLMDGLNHRFSGVINVSRIKNPIDLARALQESPDRVLSDS
CCCCCCCHHEECCCCEEEHHHHHHHHHHHHHCEEEHHHCCCHHHHHHHHHHCHHHHHCCC
GAAEFVRELQMPVYNPLTDKRLNEWLEDRKQNFNRKMANVVTVSPGTGTIGVVALDKEGR
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCC
LAAGTSTGGKGFEKIGRVSDSAMPAGNYATDSAAVSCTGIGEDIIDECLAAKIVIRVTDG
EEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEEEEECC
FSIKAAFERSFAEAHEHKRDLGAIGIDAGGTINWGKTCDVILAAFHDGEKIGDSLEADKG
CEEHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCC
KSTFSVE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]