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The map label for this gene is metY [H]

Identifier: 110835416

GI number: 110835416

Start: 2894827

End: 2896167

Strand: Reverse

Name: metY [H]

Synonym: ABO_2555

Alternate gene names: 110835416

Gene position: 2896167-2894827 (Counterclockwise)

Preceding gene: 110835417

Following gene: 110835413

Centisome position: 92.82

GC content: 54.06

Gene sequence:

>1341_bases
TTGGGCCGCCGCTTGGCGGCCTTTTTGCGTGTGCGTCGTTTTGCGGCGAAAATTATGAGGCAGAGAGTGATGAAAAAAGA
AACCATTGCCATTCATGGGGGCTTTGCCGGTGACCCGCAGACCCATGCGGTGGCCGTACCCATGTATCAAACCACCAGTT
ATTACTTTGATGATACCCAGCATGGGGCGGATCTATTTGACCTGAAAGTACCGGGCAATATTTATAGCCGCATCATGAAC
CCGACTAACGCTGTGCTGGAAGAGCGCGTTGCGCAACTGGAAGGGGGCATTGGTGCCTTGGCGATGGCGTCGGGCATGGC
GGCGATTACCGCGACGATCCAAACGCTGGCAAAGGCGGGAGACAATATTGTTTCAACCAGCCAGCTTTATGGCGGTACCT
ACAATTTGTTTGCGCATACCTTGCCGAACCAGGGTATTGAGGTGCGCATGGCCGATGGCCGCAATCCTCAGGCGATTGCG
GAGGCTATTGATGAAAAAACGAAGATGGTGTTCTGCGAATCCATCGGCAACCCGGCGGGCAATGTGGTCGATATGGCTGC
GTTGGCTAAGGTAGCCCATGACGCGGGTGTACCGCTTGTAGTTGATAACACCGTGGCAACGCCTGCATTGTGCCGACCCT
TTGAGCATGGCGCTGATATTGTGGTGCATTCGCTGACCAAATACATGGGCGGACATGGCACCACCATTGCTGGCGCTATC
GTCGATTCAGGGAAGTTTCCATGGAAAGGAAACCCTCGCTTCCCGCAGTTTAATGAGCCGGATCCAAGCTATCATGGTGT
GGTGTACGTGGAGGCCATGGGAGAGGCGGCGTTTATTGGTCGTGCTCGGGTTGTCCCACTGCGTAATATGGGGGCAGCGT
TATCTCCTATGAATGCGTTTTTAATTATGCAGGGCATTGAAACCTTGAGCCTGCGGATGGAACGGCATTGCGAGAACGCG
GTGAAAGTGGCGACATACTTGCGTGATCATGCCAAGGTGAGTTGGGTTAATTTTGCCGGGCTGGAAACCGACAAAGATTA
TGGTCTAGCGCAGCAGTACATGGATGGAGGAGTGCCCTCGTCTATTCTCAGCTTTGGTGTGCAAGGGGGGCGTGATGCCG
GCGCGCGCTTTATTGATGCGTTGGCATTGATCAAGCGGCTGGTGAATATCGGTGATGCTAAGTCTCTGGCGTGCCATCCA
GCGACCACTACGCACCGTCAGCTTAATGATGAAGAGCTGAGCAAGGCTGGGGTTAGTGCGGACTTGGTACGTTTGTCCAT
TGGTATTGAGCATGTTGACGATATCCTGGCGGATATCGAGCAGGCGTTGGCGGCGGTTTAG

Upstream 100 bases:

>100_bases
TTTTCTTGAAAAGCGCCCGCCGCGTTTTGATACTTCGCTGGCGGATGTGCCCAAACAATTTGATTTTTGGGAAGCGCCTC
CGATTAAATAAGGTGGTTTT

Downstream 100 bases:

>100_bases
TGCCTTTGAGCAAGGCTGAACGGGCCAGGGAGTATTCAGATGCTCCCTGGCCCTGAGCCTCAAATCGGCCTGTCGTCATT
CCGTGCGTTTAAAGGGTTTG

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MGRRLAAFLRVRRFAAKIMRQRVMKKETIAIHGGFAGDPQTHAVAVPMYQTTSYYFDDTQHGADLFDLKVPGNIYSRIMN
PTNAVLEERVAQLEGGIGALAMASGMAAITATIQTLAKAGDNIVSTSQLYGGTYNLFAHTLPNQGIEVRMADGRNPQAIA
EAIDEKTKMVFCESIGNPAGNVVDMAALAKVAHDAGVPLVVDNTVATPALCRPFEHGADIVVHSLTKYMGGHGTTIAGAI
VDSGKFPWKGNPRFPQFNEPDPSYHGVVYVEAMGEAAFIGRARVVPLRNMGAALSPMNAFLIMQGIETLSLRMERHCENA
VKVATYLRDHAKVSWVNFAGLETDKDYGLAQQYMDGGVPSSILSFGVQGGRDAGARFIDALALIKRLVNIGDAKSLACHP
ATTTHRQLNDEELSKAGVSADLVRLSIGIEHVDDILADIEQALAAV

Sequences:

>Translated_446_residues
MGRRLAAFLRVRRFAAKIMRQRVMKKETIAIHGGFAGDPQTHAVAVPMYQTTSYYFDDTQHGADLFDLKVPGNIYSRIMN
PTNAVLEERVAQLEGGIGALAMASGMAAITATIQTLAKAGDNIVSTSQLYGGTYNLFAHTLPNQGIEVRMADGRNPQAIA
EAIDEKTKMVFCESIGNPAGNVVDMAALAKVAHDAGVPLVVDNTVATPALCRPFEHGADIVVHSLTKYMGGHGTTIAGAI
VDSGKFPWKGNPRFPQFNEPDPSYHGVVYVEAMGEAAFIGRARVVPLRNMGAALSPMNAFLIMQGIETLSLRMERHCENA
VKVATYLRDHAKVSWVNFAGLETDKDYGLAQQYMDGGVPSSILSFGVQGGRDAGARFIDALALIKRLVNIGDAKSLACHP
ATTTHRQLNDEELSKAGVSADLVRLSIGIEHVDDILADIEQALAAV
>Mature_445_residues
GRRLAAFLRVRRFAAKIMRQRVMKKETIAIHGGFAGDPQTHAVAVPMYQTTSYYFDDTQHGADLFDLKVPGNIYSRIMNP
TNAVLEERVAQLEGGIGALAMASGMAAITATIQTLAKAGDNIVSTSQLYGGTYNLFAHTLPNQGIEVRMADGRNPQAIAE
AIDEKTKMVFCESIGNPAGNVVDMAALAKVAHDAGVPLVVDNTVATPALCRPFEHGADIVVHSLTKYMGGHGTTIAGAIV
DSGKFPWKGNPRFPQFNEPDPSYHGVVYVEAMGEAAFIGRARVVPLRNMGAALSPMNAFLIMQGIETLSLRMERHCENAV
KVATYLRDHAKVSWVNFAGLETDKDYGLAQQYMDGGVPSSILSFGVQGGRDAGARFIDALALIKRLVNIGDAKSLACHPA
TTTHRQLNDEELSKAGVSADLVRLSIGIEHVDDILADIEQALAAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=427, Percent_Identity=32.5526932084309, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI299473759, Length=427, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI25453487, Length=426, Percent_Identity=28.8732394366197, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1790375, Length=421, Percent_Identity=31.353919239905, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789383, Length=431, Percent_Identity=24.8259860788863, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71980708, Length=410, Percent_Identity=30.7317073170732, Blast_Score=191, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=419, Percent_Identity=33.4128878281623, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=419, Percent_Identity=32.4582338902148, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=49.7695852534562, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319307, Length=374, Percent_Identity=33.1550802139037, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=449, Percent_Identity=26.5033407572383, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321904, Length=427, Percent_Identity=24.3559718969555, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321170, Length=201, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922554, Length=420, Percent_Identity=32.1428571428571, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47874; Mature: 47743

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRRLAAFLRVRRFAAKIMRQRVMKKETIAIHGGFAGDPQTHAVAVPMYQTTSYYFDDTQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEEEECCEEEECCCC
HGADLFDLKVPGNIYSRIMNPTNAVLEERVAQLEGGIGALAMASGMAAITATIQTLAKAG
CCCCEEEEECCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
DNIVSTSQLYGGTYNLFAHTLPNQGIEVRMADGRNPQAIAEAIDEKTKMVFCESIGNPAG
CCCEEHHHHCCCCHHHHEECCCCCCCEEEECCCCCHHHHHHHHHHHHHEEEEHHCCCCCC
NVVDMAALAKVAHDAGVPLVVDNTVATPALCRPFEHGADIVVHSLTKYMGGHGTTIAGAI
CHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
VDSGKFPWKGNPRFPQFNEPDPSYHGVVYVEAMGEAAFIGRARVVPLRNMGAALSPMNAF
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCEEEEEHHHCCCHHCHHHHH
LIMQGIETLSLRMERHCENAVKVATYLRDHAKVSWVNFAGLETDKDYGLAQQYMDGGVPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHCCCCCH
SILSFGVQGGRDAGARFIDALALIKRLVNIGDAKSLACHPATTTHRQLNDEELSKAGVSA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHCCCHHHHHHCCCCH
DLVRLSIGIEHVDDILADIEQALAAV
HHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GRRLAAFLRVRRFAAKIMRQRVMKKETIAIHGGFAGDPQTHAVAVPMYQTTSYYFDDTQ
CHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEEEECCEEEECCCC
HGADLFDLKVPGNIYSRIMNPTNAVLEERVAQLEGGIGALAMASGMAAITATIQTLAKAG
CCCCEEEEECCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
DNIVSTSQLYGGTYNLFAHTLPNQGIEVRMADGRNPQAIAEAIDEKTKMVFCESIGNPAG
CCCEEHHHHCCCCHHHHEECCCCCCCEEEECCCCCHHHHHHHHHHHHHEEEEHHCCCCCC
NVVDMAALAKVAHDAGVPLVVDNTVATPALCRPFEHGADIVVHSLTKYMGGHGTTIAGAI
CHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
VDSGKFPWKGNPRFPQFNEPDPSYHGVVYVEAMGEAAFIGRARVVPLRNMGAALSPMNAF
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCEEEEEHHHCCCHHCHHHHH
LIMQGIETLSLRMERHCENAVKVATYLRDHAKVSWVNFAGLETDKDYGLAQQYMDGGVPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHCCCCCH
SILSFGVQGGRDAGARFIDALALIKRLVNIGDAKSLACHPATTTHRQLNDEELSKAGVSA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHCCCHHHHHHCCCCH
DLVRLSIGIEHVDDILADIEQALAAV
HHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]