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The map label for this gene is leuA [H]

Identifier: 110835298

GI number: 110835298

Start: 2758502

End: 2760181

Strand: Reverse

Name: leuA [H]

Synonym: ABO_2437

Alternate gene names: 110835298

Gene position: 2760181-2758502 (Counterclockwise)

Preceding gene: 110835301

Following gene: 110835294

Centisome position: 88.46

GC content: 56.19

Gene sequence:

>1680_bases
ATGAGCTTTGATCACCGTAAATATCGTCCTGTTGCCGTTATCGACAAACCCGACCGTCGCTGGCCTAACCAGCGTATTGA
GAAGGCACCGCTGTGGGCAGCAGTGGACCTACGCGATGGTAACCAGGCGCTGATCAAGCCCATGTCGGTGGCGCAAAAGC
GTCGTTTTTTCCAAATGTTGGTGGAGCTGGGTTTCAAGGAAATCGAAGTGGGTTTTCCGTCAGCCAGCCAGATCGATTTT
GATTTTTGTCGGGCCTTGATTGAAGAAGATCTGGTGCCCGATGACGTACATATCCAAGTGCTGACTCAGGCCCGAGAAGA
CTTGATTGCGCGTACCTTTGACTCTCTCAAAGGGGCGAAGAACGCCATTGTGCATATTTACAATGCCACCTCGCCCACTT
TCCGCGAGCAGGTGTTCAACGTGGACAAGGCCGGTTGTAAGGCGATTGCCGTGCGGGCCGCTGAATGGGTGAAAGAGAAC
GCGGCCAAGCAGCCGGATACCCACTGGAGTTTCCAGTATTCCCCGGAAACGTTCAGTGCCACGGAGACCGATTTCGCCAT
TGAAGTGATTGATGCGGTGAATGCGGTATGGCGACCGGATCAGGGGCAGCGAGTAATCATTAACCTGCCGGCTACGGTGG
AAGTGAGCACGCCGAATGTGTTCGCGGACCAGGTAGAGATGGTGCACGACAATATTCAGTATCGCGATGATGTGATCATC
AGCGTGCACACCCATGATGACCGTGGTTGTGGTGTGGCTGCAGCGGAAATGGCGGTAATGGCAGGGGCTGACCGGGTGGA
GGGGACCTTGTTAGGTAATGGTGAGCGTACCGGCAATATGGACCTGGTGACTGCAGGCATGAACCTGTACAGCCAGGGAA
TAGACCCGGGAATTGACTTCTCGCGCATGAAAGAGATTGTGGCGTTGGTGGAAGAGATTACCGACATCCAAACTCATCCC
CGCCACCCCTATGCGGGTGACCTGGTGTTCAGTGCTTTCTCCGGCAGCCATCAGGATGCCATCCGCAAATGCTTGGCCCG
CTATCAGGAAGGCGATATCTGGACAGCGGCTTACTTGCCCATCGATCCGGCAGATGTGGGGCGGCGCTACGAGGAAGTGG
TACGGATTAACTCCCAGTCCGGTAAGGGTGGCGTAGCTCACGTGCTGGAGCGGGATTTCGGTATCGATTTGCCGCGCTGG
TTGCAGCAGGAGCTTGCCGGTGTGGTGCAGGGTGACGCCGAAGAAGATGGTGGCGAGATAACCAGCGAACGAGTGCACCG
CCGTTTCAATAGCGATTATCTGAACGTGCCCATGGGTTGGGTATTGCGTTCCTACGACTTGAACCGCAGCAACGAACAGG
TGCAGGCGCAGATCAGTATCGGTGATGATCGTCAGCCGGTGACCCTGTTGTCTGGCCGCGGTGACGGCGCCATGTCGGCC
CTGGTGGATGCGCTTAACCGGCGTATCGGTGGTGAGGTGAAGGTGGTGTCTTTCGATGAATATTCGCTGGGCGATAACAC
CGAAGCCAACGCCATGGCCTGTGTGCGGGTACAGGTGGGTGACTCCACGCAAAGTGCTGTAGCCATGGCGGTGGATACTA
CCGCTGCGGCCTTGCAGGCCATTCTTTCTGCGGTGGGGCGAATGCAGGAAACGAGTGAGCAGTTAATCGCTAACAGTTAG

Upstream 100 bases:

>100_bases
GCGAGTTGTTAACCAAGTCAGACAGTACCCCTGAACGATCAGGTTAGGAATTCACTTTCGGGGGGCAGGACATGCCCCTT
TTTACGTTAAGGAAATTGTC

Downstream 100 bases:

>100_bases
TCGCGGCGCCGCCGCATTGGTTGTTGGGCAATCGAGAAGCCGCGCTCCGGTCAGAGACGCGGCTTCTTGTTTTGTAGAAG
GGGCAGCGCGCAGGTCGAAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MSFDHRKYRPVAVIDKPDRRWPNQRIEKAPLWAAVDLRDGNQALIKPMSVAQKRRFFQMLVELGFKEIEVGFPSASQIDF
DFCRALIEEDLVPDDVHIQVLTQAREDLIARTFDSLKGAKNAIVHIYNATSPTFREQVFNVDKAGCKAIAVRAAEWVKEN
AAKQPDTHWSFQYSPETFSATETDFAIEVIDAVNAVWRPDQGQRVIINLPATVEVSTPNVFADQVEMVHDNIQYRDDVII
SVHTHDDRGCGVAAAEMAVMAGADRVEGTLLGNGERTGNMDLVTAGMNLYSQGIDPGIDFSRMKEIVALVEEITDIQTHP
RHPYAGDLVFSAFSGSHQDAIRKCLARYQEGDIWTAAYLPIDPADVGRRYEEVVRINSQSGKGGVAHVLERDFGIDLPRW
LQQELAGVVQGDAEEDGGEITSERVHRRFNSDYLNVPMGWVLRSYDLNRSNEQVQAQISIGDDRQPVTLLSGRGDGAMSA
LVDALNRRIGGEVKVVSFDEYSLGDNTEANAMACVRVQVGDSTQSAVAMAVDTTAAALQAILSAVGRMQETSEQLIANS

Sequences:

>Translated_559_residues
MSFDHRKYRPVAVIDKPDRRWPNQRIEKAPLWAAVDLRDGNQALIKPMSVAQKRRFFQMLVELGFKEIEVGFPSASQIDF
DFCRALIEEDLVPDDVHIQVLTQAREDLIARTFDSLKGAKNAIVHIYNATSPTFREQVFNVDKAGCKAIAVRAAEWVKEN
AAKQPDTHWSFQYSPETFSATETDFAIEVIDAVNAVWRPDQGQRVIINLPATVEVSTPNVFADQVEMVHDNIQYRDDVII
SVHTHDDRGCGVAAAEMAVMAGADRVEGTLLGNGERTGNMDLVTAGMNLYSQGIDPGIDFSRMKEIVALVEEITDIQTHP
RHPYAGDLVFSAFSGSHQDAIRKCLARYQEGDIWTAAYLPIDPADVGRRYEEVVRINSQSGKGGVAHVLERDFGIDLPRW
LQQELAGVVQGDAEEDGGEITSERVHRRFNSDYLNVPMGWVLRSYDLNRSNEQVQAQISIGDDRQPVTLLSGRGDGAMSA
LVDALNRRIGGEVKVVSFDEYSLGDNTEANAMACVRVQVGDSTQSAVAMAVDTTAAALQAILSAVGRMQETSEQLIANS
>Mature_558_residues
SFDHRKYRPVAVIDKPDRRWPNQRIEKAPLWAAVDLRDGNQALIKPMSVAQKRRFFQMLVELGFKEIEVGFPSASQIDFD
FCRALIEEDLVPDDVHIQVLTQAREDLIARTFDSLKGAKNAIVHIYNATSPTFREQVFNVDKAGCKAIAVRAAEWVKENA
AKQPDTHWSFQYSPETFSATETDFAIEVIDAVNAVWRPDQGQRVIINLPATVEVSTPNVFADQVEMVHDNIQYRDDVIIS
VHTHDDRGCGVAAAEMAVMAGADRVEGTLLGNGERTGNMDLVTAGMNLYSQGIDPGIDFSRMKEIVALVEEITDIQTHPR
HPYAGDLVFSAFSGSHQDAIRKCLARYQEGDIWTAAYLPIDPADVGRRYEEVVRINSQSGKGGVAHVLERDFGIDLPRWL
QQELAGVVQGDAEEDGGEITSERVHRRFNSDYLNVPMGWVLRSYDLNRSNEQVQAQISIGDDRQPVTLLSGRGDGAMSAL
VDALNRRIGGEVKVVSFDEYSLGDNTEANAMACVRVQVGDSTQSAVAMAVDTTAAALQAILSAVGRMQETSEQLIANS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=539, Percent_Identity=25.7884972170686, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324682, Length=569, Percent_Identity=44.2882249560633, Blast_Score=490, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6324225, Length=562, Percent_Identity=43.9501779359431, Blast_Score=479, Evalue=1e-136,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 61823; Mature: 61692

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDHRKYRPVAVIDKPDRRWPNQRIEKAPLWAAVDLRDGNQALIKPMSVAQKRRFFQML
CCCCCCCCCCEEEEECCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VELGFKEIEVGFPSASQIDFDFCRALIEEDLVPDDVHIQVLTQAREDLIARTFDSLKGAK
HHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NAIVHIYNATSPTFREQVFNVDKAGCKAIAVRAAEWVKENAAKQPDTHWSFQYSPETFSA
CEEEEEECCCCCHHHHHHHCCCHHCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
TETDFAIEVIDAVNAVWRPDQGQRVIINLPATVEVSTPNVFADQVEMVHDNIQYRDDVII
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCEEE
SVHTHDDRGCGVAAAEMAVMAGADRVEGTLLGNGERTGNMDLVTAGMNLYSQGIDPGIDF
EEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCHHHHHCCCCCCCCH
SRMKEIVALVEEITDIQTHPRHPYAGDLVFSAFSGSHQDAIRKCLARYQEGDIWTAAYLP
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHCCCCCEEEEEECC
IDPADVGRRYEEVVRINSQSGKGGVAHVLERDFGIDLPRWLQQELAGVVQGDAEEDGGEI
CCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCH
TSERVHRRFNSDYLNVPMGWVLRSYDLNRSNEQVQAQISIGDDRQPVTLLSGRGDGAMSA
HHHHHHHHHCCCEECCCHHHHEEECCCCCCCCEEEEEEEECCCCCCEEEEECCCCHHHHH
LVDALNRRIGGEVKVVSFDEYSLGDNTEANAMACVRVQVGDSTQSAVAMAVDTTAAALQA
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHEEEEHHHHHHHHHH
ILSAVGRMQETSEQLIANS
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFDHRKYRPVAVIDKPDRRWPNQRIEKAPLWAAVDLRDGNQALIKPMSVAQKRRFFQML
CCCCCCCCCEEEEECCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VELGFKEIEVGFPSASQIDFDFCRALIEEDLVPDDVHIQVLTQAREDLIARTFDSLKGAK
HHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NAIVHIYNATSPTFREQVFNVDKAGCKAIAVRAAEWVKENAAKQPDTHWSFQYSPETFSA
CEEEEEECCCCCHHHHHHHCCCHHCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
TETDFAIEVIDAVNAVWRPDQGQRVIINLPATVEVSTPNVFADQVEMVHDNIQYRDDVII
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCEEE
SVHTHDDRGCGVAAAEMAVMAGADRVEGTLLGNGERTGNMDLVTAGMNLYSQGIDPGIDF
EEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCHHHHHCCCCCCCCH
SRMKEIVALVEEITDIQTHPRHPYAGDLVFSAFSGSHQDAIRKCLARYQEGDIWTAAYLP
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHCCCCCEEEEEECC
IDPADVGRRYEEVVRINSQSGKGGVAHVLERDFGIDLPRWLQQELAGVVQGDAEEDGGEI
CCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCH
TSERVHRRFNSDYLNVPMGWVLRSYDLNRSNEQVQAQISIGDDRQPVTLLSGRGDGAMSA
HHHHHHHHHCCCEECCCHHHHEEECCCCCCCCEEEEEEEECCCCCCEEEEECCCCHHHHH
LVDALNRRIGGEVKVVSFDEYSLGDNTEANAMACVRVQVGDSTQSAVAMAVDTTAAALQA
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHEEEEHHHHHHHHHH
ILSAVGRMQETSEQLIANS
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA