Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 110835301

GI number: 110835301

Start: 2761782

End: 2762555

Strand: Reverse

Name: paaF [C]

Synonym: ABO_2440

Alternate gene names: 110835301

Gene position: 2762555-2761782 (Counterclockwise)

Preceding gene: 110835302

Following gene: 110835298

Centisome position: 88.54

GC content: 54.65

Gene sequence:

>774_bases
GTGACGATGCCGACACTGGAAAGTGATCTGGTTCTGGTGAATAACACCGAATCGGTAATGGAAATTACCCTGAACCGGCC
GGACAAGTTGAATGCGCTGACAAATGCCATGTATCGGGATCTGGTTAAGCTGTTGAAAGCCGCTGAGCAGAATGAGGCTA
TTCATGTCGTGCTGTTCCGTGGTGAGGGAAAGTCCTTCAGTGCGGGTAACGACTTGGTGGATTTCCTCAACGCGACACCG
GGTGAGGCCGGCGGGGCCGCCCATTTCATTCAGGCCATTCATGATTTCCCCAAGGTGGTCGTTGCGGCGGTGCAGGGTAA
TGCGGTGGGCCTAGGCACCACCATGTTGTTGCATATGGACTTGGTGGTGGCGTCCGCGGATGCCAAATTGGTTACTCCTT
TTGTGGATCTGGGGGTGGCATCAGAAGCGGGTTCCTCCAAGCTGCTACCTGCGTGGATCGGTTATCAGCGTGCCGCGCGG
ATGCTATTGCTGGGTGAGCCCATGCTGGCTCAGGAAGCGCTGGAAATAGGCCTGGTGGCCAAGGTTGTCGAGCGTGAAGA
GCTGGATGCCACCGCGCGTAATATGGCGGCGATATTGGCGAAGAAACCTCCTCGCGCATTGCAAGCCAGTAAACGATTGA
TGCGAGAATCCATCAATAAGCCTTTGCATCAGGTGATCAAGGAAGACCTGGCGCTGTTTGCCGAAATGCTTCAGGGCGAT
GAGGCAAAAGGCGTTATTGCGGCTATGGTGAACAAGAGTAAAGGCTCGAAGTAA

Upstream 100 bases:

>100_bases
CCTTGCTTGCCTACGACCTATGCACCGCCTTACCGGATACATAGTTAACGGTAATGCGGATTGCGGTTAAGTACTAAAGA
AAAATACTGGGAGAGCTGTT

Downstream 100 bases:

>100_bases
AGGGTAAGTTGAAATACGGAATCGCGTGGTAAGGGCTCAGGCGCCTGCCCGCGATTTACCATCGTGTGTGGAGGGGCCTT
TAGAGCGTATCAGTGCTATA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTMPTLESDLVLVNNTESVMEITLNRPDKLNALTNAMYRDLVKLLKAAEQNEAIHVVLFRGEGKSFSAGNDLVDFLNATP
GEAGGAAHFIQAIHDFPKVVVAAVQGNAVGLGTTMLLHMDLVVASADAKLVTPFVDLGVASEAGSSKLLPAWIGYQRAAR
MLLLGEPMLAQEALEIGLVAKVVEREELDATARNMAAILAKKPPRALQASKRLMRESINKPLHQVIKEDLALFAEMLQGD
EAKGVIAAMVNKSKGSK

Sequences:

>Translated_257_residues
MTMPTLESDLVLVNNTESVMEITLNRPDKLNALTNAMYRDLVKLLKAAEQNEAIHVVLFRGEGKSFSAGNDLVDFLNATP
GEAGGAAHFIQAIHDFPKVVVAAVQGNAVGLGTTMLLHMDLVVASADAKLVTPFVDLGVASEAGSSKLLPAWIGYQRAAR
MLLLGEPMLAQEALEIGLVAKVVEREELDATARNMAAILAKKPPRALQASKRLMRESINKPLHQVIKEDLALFAEMLQGD
EAKGVIAAMVNKSKGSK
>Mature_256_residues
TMPTLESDLVLVNNTESVMEITLNRPDKLNALTNAMYRDLVKLLKAAEQNEAIHVVLFRGEGKSFSAGNDLVDFLNATPG
EAGGAAHFIQAIHDFPKVVVAAVQGNAVGLGTTMLLHMDLVVASADAKLVTPFVDLGVASEAGSSKLLPAWIGYQRAARM
LLLGEPMLAQEALEIGLVAKVVEREELDATARNMAAILAKKPPRALQASKRLMRESINKPLHQVIKEDLALFAEMLQGDE
AKGVIAAMVNKSKGSK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=29.2181069958848, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=29.6442687747036, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI25453481, Length=244, Percent_Identity=26.6393442622951, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI51538573, Length=244, Percent_Identity=26.6393442622951, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4757966, Length=244, Percent_Identity=26.6393442622951, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538581, Length=244, Percent_Identity=26.6393442622951, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI68989263, Length=270, Percent_Identity=27.4074074074074, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4757968, Length=181, Percent_Identity=29.2817679558011, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI49355787, Length=181, Percent_Identity=29.2817679558011, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI221136756, Length=202, Percent_Identity=28.2178217821782, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=202, Percent_Identity=28.2178217821782, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI31542718, Length=194, Percent_Identity=31.4432989690722, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221307494, Length=215, Percent_Identity=26.5116279069767, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI116235487, Length=240, Percent_Identity=25.8333333333333, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=246, Percent_Identity=32.1138211382114, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787660, Length=219, Percent_Identity=34.703196347032, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI221142681, Length=218, Percent_Identity=32.1100917431193, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87082183, Length=215, Percent_Identity=27.906976744186, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788597, Length=215, Percent_Identity=25.5813953488372, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=29.0502793296089, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788682, Length=187, Percent_Identity=28.3422459893048, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17549921, Length=215, Percent_Identity=35.8139534883721, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17535521, Length=271, Percent_Identity=31.3653136531365, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25145438, Length=238, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=281, Percent_Identity=27.0462633451957, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=221, Percent_Identity=31.6742081447964, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=221, Percent_Identity=27.6018099547511, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=229, Percent_Identity=27.0742358078603, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=188, Percent_Identity=31.3829787234043, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323314, Length=189, Percent_Identity=27.5132275132275, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=210, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24654903, Length=221, Percent_Identity=30.7692307692308, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI20129971, Length=217, Percent_Identity=29.4930875576037, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653477, Length=217, Percent_Identity=29.4930875576037, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922422, Length=186, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920382, Length=218, Percent_Identity=27.5229357798165, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357171, Length=249, Percent_Identity=27.3092369477912, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24653139, Length=189, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27548; Mature: 27416

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPTLESDLVLVNNTESVMEITLNRPDKLNALTNAMYRDLVKLLKAAEQNEAIHVVLFR
CCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
GEGKSFSAGNDLVDFLNATPGEAGGAAHFIQAIHDFPKVVVAAVQGNAVGLGTTMLLHMD
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LVVASADAKLVTPFVDLGVASEAGSSKLLPAWIGYQRAARMLLLGEPMLAQEALEIGLVA
HHHCCCCCHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KVVEREELDATARNMAAILAKKPPRALQASKRLMRESINKPLHQVIKEDLALFAEMLQGD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EAKGVIAAMVNKSKGSK
CCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TMPTLESDLVLVNNTESVMEITLNRPDKLNALTNAMYRDLVKLLKAAEQNEAIHVVLFR
CCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
GEGKSFSAGNDLVDFLNATPGEAGGAAHFIQAIHDFPKVVVAAVQGNAVGLGTTMLLHMD
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LVVASADAKLVTPFVDLGVASEAGSSKLLPAWIGYQRAARMLLLGEPMLAQEALEIGLVA
HHHCCCCCHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KVVEREELDATARNMAAILAKKPPRALQASKRLMRESINKPLHQVIKEDLALFAEMLQGD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EAKGVIAAMVNKSKGSK
CCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA