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The map label for this gene is speE [H]

Identifier: 110835260

GI number: 110835260

Start: 2721265

End: 2722125

Strand: Direct

Name: speE [H]

Synonym: ABO_2399

Alternate gene names: 110835260

Gene position: 2721265-2722125 (Clockwise)

Preceding gene: 110835256

Following gene: 110835264

Centisome position: 87.22

GC content: 57.61

Gene sequence:

>861_bases
ATGAGCAGCACCACAAAAAACTGGTTTTACGAGCACTTCGACTCCGCTGGCACTGCCTTCGGCCTGCGCCTGAAACAAAA
GCTGGAAGAAGTACAAACCGAGTACCAGCACATCGAGATGTGGGAAACCGACACCTTCGGTTACCTGATGACCATCGATG
GCGCCACCATGCTTTCCACCCGCGACAACTTTCTCTACCACGAGATGATGTCGCACCCGGTACTGTTCAGCCATGCCAGC
CCCAAGCGCGTCATCATTATTGGTGGCGGCGACTGCGGCACCCTGCGTGAAGTGCTGCGTCATCCGAGCGTGGAAAAAGC
CACCCAGATCGACATCGACGAAATGGTCACCCGCTATTCCGAGAAATACTTCCCAGAATTGTGCGAGTCCAACGATGACA
GCCGTGCCGAGCTATTGTTCGAAGATGGCGTGCAATACATGCGCAACTGTGAAGACGGCAGCGTGGACGTGATCATCGTC
GATTCCACTGACCCGGTGGGCCCGGCGGAAGGTTTGTTCAAAGCCGACTTCTTCCGCGATTGCCACCGCGTGCTGGCCGA
CGGCGGCATCATCGTGCAGCAATCTGAATCCCCGCTGCTGCACAGCGACACCATCATTAAAGACCTGCATACCAACATGC
GCGAAGCGGGCTTCACCACCACCCAAACCCTGCCCTTCCCGCAGCCGGTGTACCCCAGCGGCTGGTGGAGCTGCACCCTG
GCGGGAAAAAACACCGACGTGAGCCGCTTCCGGGAAGCCGATGCCACCGGCAAAGGGTTTGATACCCGCTACTATTCAGC
GGCGATTCATAAAGGGGCGTTGGTGATGCCGCCGTTTATGGAAGCGGCGATTAAGCGCTGA

Upstream 100 bases:

>100_bases
AGCACCCATTTTTCATGCTTTCACAGGAGCCGTTCTGGTCTTACAATCGCCCCTATTGTCCACCACGGAGCCCGCCCGCT
CCCAGCCACCGGAATCGCCC

Downstream 100 bases:

>100_bases
ATTGAACGTTAAAAAAATGCCCTTGTCTTCGCTGGAAGCAAGGGCATTTTTATGAAGAAGACTTTTGGGAAACGGGAGAC
TTCACAATCATGCCTTCTCA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSSTTKNWFYEHFDSAGTAFGLRLKQKLEEVQTEYQHIEMWETDTFGYLMTIDGATMLSTRDNFLYHEMMSHPVLFSHAS
PKRVIIIGGGDCGTLREVLRHPSVEKATQIDIDEMVTRYSEKYFPELCESNDDSRAELLFEDGVQYMRNCEDGSVDVIIV
DSTDPVGPAEGLFKADFFRDCHRVLADGGIIVQQSESPLLHSDTIIKDLHTNMREAGFTTTQTLPFPQPVYPSGWWSCTL
AGKNTDVSRFREADATGKGFDTRYYSAAIHKGALVMPPFMEAAIKR

Sequences:

>Translated_286_residues
MSSTTKNWFYEHFDSAGTAFGLRLKQKLEEVQTEYQHIEMWETDTFGYLMTIDGATMLSTRDNFLYHEMMSHPVLFSHAS
PKRVIIIGGGDCGTLREVLRHPSVEKATQIDIDEMVTRYSEKYFPELCESNDDSRAELLFEDGVQYMRNCEDGSVDVIIV
DSTDPVGPAEGLFKADFFRDCHRVLADGGIIVQQSESPLLHSDTIIKDLHTNMREAGFTTTQTLPFPQPVYPSGWWSCTL
AGKNTDVSRFREADATGKGFDTRYYSAAIHKGALVMPPFMEAAIKR
>Mature_285_residues
SSTTKNWFYEHFDSAGTAFGLRLKQKLEEVQTEYQHIEMWETDTFGYLMTIDGATMLSTRDNFLYHEMMSHPVLFSHASP
KRVIIIGGGDCGTLREVLRHPSVEKATQIDIDEMVTRYSEKYFPELCESNDDSRAELLFEDGVQYMRNCEDGSVDVIIVD
STDPVGPAEGLFKADFFRDCHRVLADGGIIVQQSESPLLHSDTIIKDLHTNMREAGFTTTQTLPFPQPVYPSGWWSCTLA
GKNTDVSRFREADATGKGFDTRYYSAAIHKGALVMPPFMEAAIKR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=290, Percent_Identity=34.1379310344828, Blast_Score=174, Evalue=9e-44,
Organism=Escherichia coli, GI1786312, Length=287, Percent_Identity=34.4947735191638, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25154106, Length=275, Percent_Identity=36.7272727272727, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI212645616, Length=324, Percent_Identity=31.7901234567901, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325326, Length=276, Percent_Identity=36.5942028985507, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323175, Length=290, Percent_Identity=32.4137931034483, Blast_Score=159, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24645443, Length=288, Percent_Identity=32.2916666666667, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21355941, Length=218, Percent_Identity=32.5688073394495, Blast_Score=124, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32339; Mature: 32208

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTTKNWFYEHFDSAGTAFGLRLKQKLEEVQTEYQHIEMWETDTFGYLMTIDGATMLST
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCEEECC
RDNFLYHEMMSHPVLFSHASPKRVIIIGGGDCGTLREVLRHPSVEKATQIDIDEMVTRYS
CCHHHHHHHHCCCEEEECCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHH
EKYFPELCESNDDSRAELLFEDGVQYMRNCEDGSVDVIIVDSTDPVGPAEGLFKADFFRD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
CHRVLADGGIIVQQSESPLLHSDTIIKDLHTNMREAGFTTTQTLPFPQPVYPSGWWSCTL
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
AGKNTDVSRFREADATGKGFDTRYYSAAIHKGALVMPPFMEAAIKR
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCEECCHHHHHHHCC
>Mature Secondary Structure 
SSTTKNWFYEHFDSAGTAFGLRLKQKLEEVQTEYQHIEMWETDTFGYLMTIDGATMLST
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCEEECC
RDNFLYHEMMSHPVLFSHASPKRVIIIGGGDCGTLREVLRHPSVEKATQIDIDEMVTRYS
CCHHHHHHHHCCCEEEECCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHH
EKYFPELCESNDDSRAELLFEDGVQYMRNCEDGSVDVIIVDSTDPVGPAEGLFKADFFRD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
CHRVLADGGIIVQQSESPLLHSDTIIKDLHTNMREAGFTTTQTLPFPQPVYPSGWWSCTL
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
AGKNTDVSRFREADATGKGFDTRYYSAAIHKGALVMPPFMEAAIKR
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]