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The map label for this gene is cobB [H]

Identifier: 110835228

GI number: 110835228

Start: 2690993

End: 2692369

Strand: Reverse

Name: cobB [H]

Synonym: ABO_2367

Alternate gene names: 110835228

Gene position: 2692369-2690993 (Counterclockwise)

Preceding gene: 110835229

Following gene: 110835227

Centisome position: 86.29

GC content: 58.9

Gene sequence:

>1377_bases
ATGAGCCACCCGATGACAACCTTATTTAATGCCTCGGTGCCGGCAGCGCTGATTAGCGCGCCGGGGTCCGGTCAGGGAAA
ATCACTGATTACCGCCGCACTGGCCAGGTTGCACCGCAATGCGGGGCGTCGGGTTCGTGTGTTTAAGTTCGGGCCGGACT
ATCTGGATCCCATGATTCTGGAACAGGCCAGCGGTGCGCCGGTTTACCAGCTACAACCGTGGATGACCGGTGAGGAGGAG
TGCCGTTGGCGCCTGGCCCAGGCTGCAGAAGAGGCGGATTTGATTCTGGTAGAAGGCGCCATGGGGCTATTTGATGGCAC
TCCCTCCAGTGCTGATCTGGCGGTGTTGGCAGGTATCCCGGCGTTGCCAGTGATTGATTCCTGGGGCATGGCGCAAACCT
TCGGTGCGATCGCTTTGGGGCTGGCCAGTTACCGCGATGACCTGGTGGTGAATGAGGTTATTGCCAACCGGGTGGCGAGC
CAGCGTCATGGTGACTTGGTACGTGAAGGGATGCCTGCGGGCATGACCTTGCTGGGCTCGGTGCCACGGCACGCGGCCAT
GACATTACCCGAACGGCACCTTGGGCTGGTGCAAGCGAATGAGTTGGCGGATCTGGATACGCTTTTGGATGAGGCGGCGG
CGGTGTTGCAAGACGCGGGCTTGGATCGACTTCCACACGCGGTGAGTTTTTCCGCCGCCGCGCCTGCGCCATTGCCTGCT
TTGCTGAAGGGGTGTCGCATTGGTATCGCTCGGGATGCGGCTTTCGCGTTTATTTACCCTGCCAATCTTGATTTGCTTCG
TGAGCTGGGAGCCGCGCTGACGTTCTTTTCCCCGTTGCAAGACACGACATTGCCCGAGGTGGACGCGCTATGGCTACCGG
GCGGTTACCCGGAACTGCACGGTGAAACGCTGGCCATGAATACGGCCATGGGTGAGGCCATTCGAGATCATCACCATGCG
GGCAAACCGATTCTGGCGGAGTGCGGTGGGTTAATGAGTTGTATGGCAACGTTGGTAGACGGTGATGAAAAGCGCCATGC
CGGTTTCGGGCTGTTGCCGGGTGAAGCGTTTTTGACCAAGAAACGGCACGGGCTGGGAATGCAGGCGCTGGTAACGCCGC
GAGGTGAACTTCGCGGGCATACTTTCCACCACGGCAGTTTGTCCACGCCACTGACGCCGGCCATTTTTAGCCGCAAGCAA
ACAGGTACAGAAGGCGAAGCGGTTTATCAGGACAAGGGCCTGGTGGCGAGTTTCTTTCATGGCTATTTCCCTTCGGCTCC
GCAGATTGTGGCGGATATTTTTAGCGGACAATTCCGCACCGACACACTTTCCCCTGATCACAATGCCCCTTCTCGAACAG
GACACGACCATGCGTGA

Upstream 100 bases:

>100_bases
ACCAGAAACTGGTGGTGGCTCACGTGAACGGTGCGCCGGTCTTTGTGCCGGGTTAGGCCGCCTCTGGATAACGCTTTACG
AGCGAGGAAAATAAACGAGA

Downstream 100 bases:

>100_bases
TGATGCGAAAACCCCAGAGCGCCATGCTTACCGCATGGCAAAGAAACAGGAAATCATGCAGCAACGGATTGCTCGCGCGG
ATAAGGATCAGGGCATTCTT

Product: acid--ammonia (or amine) ligase-like protein

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSHPMTTLFNASVPAALISAPGSGQGKSLITAALARLHRNAGRRVRVFKFGPDYLDPMILEQASGAPVYQLQPWMTGEEE
CRWRLAQAAEEADLILVEGAMGLFDGTPSSADLAVLAGIPALPVIDSWGMAQTFGAIALGLASYRDDLVVNEVIANRVAS
QRHGDLVREGMPAGMTLLGSVPRHAAMTLPERHLGLVQANELADLDTLLDEAAAVLQDAGLDRLPHAVSFSAAAPAPLPA
LLKGCRIGIARDAAFAFIYPANLDLLRELGAALTFFSPLQDTTLPEVDALWLPGGYPELHGETLAMNTAMGEAIRDHHHA
GKPILAECGGLMSCMATLVDGDEKRHAGFGLLPGEAFLTKKRHGLGMQALVTPRGELRGHTFHHGSLSTPLTPAIFSRKQ
TGTEGEAVYQDKGLVASFFHGYFPSAPQIVADIFSGQFRTDTLSPDHNAPSRTGHDHA

Sequences:

>Translated_458_residues
MSHPMTTLFNASVPAALISAPGSGQGKSLITAALARLHRNAGRRVRVFKFGPDYLDPMILEQASGAPVYQLQPWMTGEEE
CRWRLAQAAEEADLILVEGAMGLFDGTPSSADLAVLAGIPALPVIDSWGMAQTFGAIALGLASYRDDLVVNEVIANRVAS
QRHGDLVREGMPAGMTLLGSVPRHAAMTLPERHLGLVQANELADLDTLLDEAAAVLQDAGLDRLPHAVSFSAAAPAPLPA
LLKGCRIGIARDAAFAFIYPANLDLLRELGAALTFFSPLQDTTLPEVDALWLPGGYPELHGETLAMNTAMGEAIRDHHHA
GKPILAECGGLMSCMATLVDGDEKRHAGFGLLPGEAFLTKKRHGLGMQALVTPRGELRGHTFHHGSLSTPLTPAIFSRKQ
TGTEGEAVYQDKGLVASFFHGYFPSAPQIVADIFSGQFRTDTLSPDHNAPSRTGHDHA
>Mature_457_residues
SHPMTTLFNASVPAALISAPGSGQGKSLITAALARLHRNAGRRVRVFKFGPDYLDPMILEQASGAPVYQLQPWMTGEEEC
RWRLAQAAEEADLILVEGAMGLFDGTPSSADLAVLAGIPALPVIDSWGMAQTFGAIALGLASYRDDLVVNEVIANRVASQ
RHGDLVREGMPAGMTLLGSVPRHAAMTLPERHLGLVQANELADLDTLLDEAAAVLQDAGLDRLPHAVSFSAAAPAPLPAL
LKGCRIGIARDAAFAFIYPANLDLLRELGAALTFFSPLQDTTLPEVDALWLPGGYPELHGETLAMNTAMGEAIRDHHHAG
KPILAECGGLMSCMATLVDGDEKRHAGFGLLPGEAFLTKKRHGLGMQALVTPRGELRGHTFHHGSLSTPLTPAIFSRKQT
GTEGEAVYQDKGLVASFFHGYFPSAPQIVADIFSGQFRTDTLSPDHNAPSRTGHDHA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 48711; Mature: 48580

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPMTTLFNASVPAALISAPGSGQGKSLITAALARLHRNAGRRVRVFKFGPDYLDPMIL
CCCCCCHHHCCCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHH
EQASGAPVYQLQPWMTGEEECRWRLAQAAEEADLILVEGAMGLFDGTPSSADLAVLAGIP
HHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCCCCCEEEEECCC
ALPVIDSWGMAQTFGAIALGLASYRDDLVVNEVIANRVASQRHGDLVREGMPAGMTLLGS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
VPRHAAMTLPERHLGLVQANELADLDTLLDEAAAVLQDAGLDRLPHAVSFSAAAPAPLPA
CCCHHHHCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCCCCCCCCHHH
LLKGCRIGIARDAAFAFIYPANLDLLRELGAALTFFSPLQDTTLPEVDALWLPGGYPELH
HHHHHHCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCC
GETLAMNTAMGEAIRDHHHAGKPILAECGGLMSCMATLVDGDEKRHAGFGLLPGEAFLTK
CCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHH
KRHGLGMQALVTPRGELRGHTFHHGSLSTPLTPAIFSRKQTGTEGEAVYQDKGLVASFFH
HHCCCCCEEEECCCHHHCCCEEECCCCCCCCCHHHHCCCCCCCCCCEEECCCCHHHHHHH
GYFPSAPQIVADIFSGQFRTDTLSPDHNAPSRTGHDHA
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SHPMTTLFNASVPAALISAPGSGQGKSLITAALARLHRNAGRRVRVFKFGPDYLDPMIL
CCCCCHHHCCCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHH
EQASGAPVYQLQPWMTGEEECRWRLAQAAEEADLILVEGAMGLFDGTPSSADLAVLAGIP
HHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCCCCCEEEEECCC
ALPVIDSWGMAQTFGAIALGLASYRDDLVVNEVIANRVASQRHGDLVREGMPAGMTLLGS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
VPRHAAMTLPERHLGLVQANELADLDTLLDEAAAVLQDAGLDRLPHAVSFSAAAPAPLPA
CCCHHHHCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCCCCCCCCHHH
LLKGCRIGIARDAAFAFIYPANLDLLRELGAALTFFSPLQDTTLPEVDALWLPGGYPELH
HHHHHHCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCC
GETLAMNTAMGEAIRDHHHAGKPILAECGGLMSCMATLVDGDEKRHAGFGLLPGEAFLTK
CCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHH
KRHGLGMQALVTPRGELRGHTFHHGSLSTPLTPAIFSRKQTGTEGEAVYQDKGLVASFFH
HHCCCCCEEEECCCHHHCCCEEECCCCCCCCCHHHHCCCCCCCCCCEEECCCCHHHHHHH
GYFPSAPQIVADIFSGQFRTDTLSPDHNAPSRTGHDHA
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]