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The map label for this gene is yadF [H]

Identifier: 110835222

GI number: 110835222

Start: 2685385

End: 2686029

Strand: Reverse

Name: yadF [H]

Synonym: ABO_2361

Alternate gene names: 110835222

Gene position: 2686029-2685385 (Counterclockwise)

Preceding gene: 110835223

Following gene: 110835213

Centisome position: 86.09

GC content: 52.4

Gene sequence:

>645_bases
ATGAAATCCCTACCTGATTTGTTTTCCAACAACGAAAAATGGCGCCACGAAATCGAAGAGCGCCACCCCGGGTTTTTCAA
AGCCCTGGGGGCGCAACAGAACCCTGAATATTTGTGGATCGGTTGTGCGGACTCCCGGGTGCCTGCCAATGAAGTGGTGG
GCATGATGCCCGGTGAGCTATTCGTTCATCGCAATGTGGCTAACCTGGTTAACCATACGGATTTGAATCTGCTATCGGTG
TTGCAGTTCGCCGTGGATGTTTTAAAGGTGAAACACATCATGGTGGTGGGCCATTATGGATGTGGTGGGGTGAAGGCCTC
CATGGAGGATGAGCCCCATGGCTTGATCGATAATTGGCTGCGCCAAGTGCGTGAACTGTACTTGCGTAATCGCAAGAACC
TGGCCGGGCTCGATGACCAGGCACGCTTGGACAAGATGTGCGAATTGAACGTTGCTCGGCAGGTAATCAATGTGTCGAAT
ACCACGGTGGTGCAGGAAGCTTGGCGCCGTGATCAGGCGTTGACCATTCACGGCTGGATTTACGGTATCGGTGATGGCCA
TCTGCGGGATCTGGATATTCAGATTCAGGACAACAGCGAGCTGCGCACTCTGGAAACTGAAGAATACGATAGCTGTTCAG
GCTAA

Upstream 100 bases:

>100_bases
CGGGTGAGCAGTTGGTACGGTTTATGAATATGACAGGGGGCTAGCGCGCCGATAGGTTGCGGGCTGCCTGTAGAGTGAAT
GCGCAAAAAAGCGAGGAACG

Downstream 100 bases:

>100_bases
AGCTACAAACAGCTCGATAGGAAACTGGAACGTTTCCCCGTCTGTTTTTTTACCGAGCACCTGTCGCCGTTGCCTTCCAG
AGGGAGGATGTCGCGGTTGC

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase 2 [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKSLPDLFSNNEKWRHEIEERHPGFFKALGAQQNPEYLWIGCADSRVPANEVVGMMPGELFVHRNVANLVNHTDLNLLSV
LQFAVDVLKVKHIMVVGHYGCGGVKASMEDEPHGLIDNWLRQVRELYLRNRKNLAGLDDQARLDKMCELNVARQVINVSN
TTVVQEAWRRDQALTIHGWIYGIGDGHLRDLDIQIQDNSELRTLETEEYDSCSG

Sequences:

>Translated_214_residues
MKSLPDLFSNNEKWRHEIEERHPGFFKALGAQQNPEYLWIGCADSRVPANEVVGMMPGELFVHRNVANLVNHTDLNLLSV
LQFAVDVLKVKHIMVVGHYGCGGVKASMEDEPHGLIDNWLRQVRELYLRNRKNLAGLDDQARLDKMCELNVARQVINVSN
TTVVQEAWRRDQALTIHGWIYGIGDGHLRDLDIQIQDNSELRTLETEEYDSCSG
>Mature_214_residues
MKSLPDLFSNNEKWRHEIEERHPGFFKALGAQQNPEYLWIGCADSRVPANEVVGMMPGELFVHRNVANLVNHTDLNLLSV
LQFAVDVLKVKHIMVVGHYGCGGVKASMEDEPHGLIDNWLRQVRELYLRNRKNLAGLDDQARLDKMCELNVARQVINVSN
TTVVQEAWRRDQALTIHGWIYGIGDGHLRDLDIQIQDNSELRTLETEEYDSCSG

Specific function: Unknown

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786318, Length=201, Percent_Identity=54.228855721393, Blast_Score=238, Evalue=2e-64,
Organism=Escherichia coli, GI1786534, Length=200, Percent_Identity=29.5, Blast_Score=83, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324292, Length=199, Percent_Identity=31.1557788944724, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 24402; Mature: 24402

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLPDLFSNNEKWRHEIEERHPGFFKALGAQQNPEYLWIGCADSRVPANEVVGMMPGEL
CCCCCHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCHH
FVHRNVANLVNHTDLNLLSVLQFAVDVLKVKHIMVVGHYGCGGVKASMEDEPHGLIDNWL
EEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHH
RQVRELYLRNRKNLAGLDDQARLDKMCELNVARQVINVSNTTVVQEAWRRDQALTIHGWI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEE
YGIGDGHLRDLDIQIQDNSELRTLETEEYDSCSG
EECCCCCEEEEEEEEECCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKSLPDLFSNNEKWRHEIEERHPGFFKALGAQQNPEYLWIGCADSRVPANEVVGMMPGEL
CCCCCHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCHH
FVHRNVANLVNHTDLNLLSVLQFAVDVLKVKHIMVVGHYGCGGVKASMEDEPHGLIDNWL
EEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHH
RQVRELYLRNRKNLAGLDDQARLDKMCELNVARQVINVSNTTVVQEAWRRDQALTIHGWI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEE
YGIGDGHLRDLDIQIQDNSELRTLETEEYDSCSG
EECCCCCEEEEEEEEECCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 10493123 [H]