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The map label for this gene is ptsP [H]

Identifier: 110835209

GI number: 110835209

Start: 2671524

End: 2673797

Strand: Reverse

Name: ptsP [H]

Synonym: ABO_2348

Alternate gene names: 110835209

Gene position: 2673797-2671524 (Counterclockwise)

Preceding gene: 110835210

Following gene: 110835207

Centisome position: 85.69

GC content: 56.07

Gene sequence:

>2274_bases
ATGCTTGATACTCTTCGACGTATTGTTCAGGAAGTGAACAACGCGGCGGATATCCCCAGCGCGCTGGACTTGATGGCCAA
GCGGGTACGCGATGCCATGGGTACAGAAGTGTGTTCAATCTACCTGCGTGATGAGGAAGAGCAGCGCTATATGCTGATGG
CCTCGGAAGGGTTGAAGCGCGAGGCAGTGGGTAAGGTGAGCTTGGGCTTGTCGGAGGGCCTAGTGGGGCAGGTTGGTTTG
CGTGAAGAGCCCATCAACCTGGAAGATGCCTTTACCCACCCCAAATTTCATTACCTGGCGGGAACCGGGGAAGATGCGTT
CCACGCTTTCCTGGGCGTGCCGGTGATGCACCATGGCAAGGTGCTGGGGATCATGGTGGTTCAGCAGCGCGATGTGCGCC
GCTTTGACCAGAGTGAAGAAGCCTTCCTGGTGACTATTTCTGCCCAATTATCAGCGGTGATTGCTCATGCCCATGCCTCG
GGTGTGCTATTTGACCAGCTGGGTGGCGGCGCGGATCTTCCTAGTTTTTACGACGGGCTGCCGGGGTGCCCTGGCGCGGC
TATTGGTTACGGCGTTCTGTTGTTTCCCGAGGCGGATTTGGCGTCGGTGCCGGACCGAGACGTTGAGGATGTGAGCGAAG
AAATTTCCCGCTTGGACGATGCCCTGGTACGGGTGCGTCAGGAAATTCGTGATTTGGCCGAGCGGGCTTCCCGATCTCTG
GGGGCTGAAGAGCAGGCGCTGTTTGATGTGTACCTGCGCATGCTGGACCGTCATGCTTTGCCCGCGGAAGTTATTGCGGC
CATTCGTGAAGGGCAGTGGGCCCAGGGTGCGTTACGTACAGTGATCGATGTCCACGTGCATAATTTCGAAATGATGGACG
ATCCGTACCTGCGTGAGCGAGCTGCAGACGTGCGTGATTTGGGCCGCCGAGTGCTGGCTCAGCTGCAAAGCCAAACTCGC
CGCCATATTATTTTTCCAGATGAATGCATTCTGCTCGGTGAAGATATTTCCGCTCCCATGTTGATGGAAGTGCCCCGTGA
ACGGGTGCGCGGGATTGTTACTACCCGTGGCTCGCGCAATTCGCATATGGCCATTGTTGCGAGAGCTATGGGCATTCCCA
CGGTGGTGGGTGCGCAGAATTTGCCGCTGAAAAAAATGGATGATAAGGAAATCATCGTTGATGGTTTTCGAGGGCGGGTG
GTGGCCAATGCCTCGGCGGAGCTTAAACAGCAGTTCCAGGAAATTATCGCAGAGGAAGAGAGCCTCCAGGCGGGTTTAGA
AAAGCTGCGTGACGAGCCAGCGCAAACCTTGGACGGTACGCGCATCACGTTGCAAGTGAATACTGGGTTGATGACCGATA
TCAACCGGTCACTGGAACGGGGCGCGGAAGGGGTGGGCCTGTACCGGACGGAAATTCCATTTATGGTTCGTGACCGGTTT
CCGTCGGAAGAAGAGCAACGAGTGATTTATCGCGAGCAGCTGGCGGCATTTGCTCCGCACTCGGTGACCATGCGCACCCT
GGATATCGGGGGCGATAAGTCGCTGAGTTATTTCCCTATAGAGGATGAAAACCCTTTTCTGGGCTGGCGCGGCATCCGTG
TCACCCTAGATCATCCGGAAATTTTCATGGTGCAGGTGCGTGCCATGATCAAAGCCAGTGAAGGCCTGGACAATCTGCGC
ATTATGCTGCCCATGATTACCAGCGTGATTGAAGCGGAAGAAGCGCAACACCTGATCCATCGTGCTTGGCTTGAGGTTCG
TGACGAGGGGTTGGATGTATCCATGCCGCCGGTGGGTGTGATGATCGAAGTGCCGGCCTCGGTGTACCAGGCTCGGGCGC
TGGCCCAACGGGTGGATTTTTTGTCCGTTGGCACCAATGACCTGACCCAATACCTGTTGGCGGTGGACCGCAACAACCGG
CAGGTGGCATCACTCTATAATTCCTATCACCCGGCGGTCCTGCATGCATTGATGCATGTGGTAGAGCAGGCTCGAGAAGA
ACACACTCCCGTGTCAGTTTGTGGTGAGATGGCAGGGGAGCCTAGCGCCGCCCTGCTGTTAATGGCCATGGGGGTGGATG
TGCTGTCCATGAATGCGTCCAACCTGTTGAAGGTAAAAGCCGCTATTCGGCAAGTGGATATCAGCTTTGTTCGCCGCCTT
CTCAATGAAGTGCTGGCCATGGATAACGGTGAAGTAATCGGTTCTTACGTGGATCTACAGCTCACCAAGGCTGGTCTTGG
CGAGCTGTTGCGAAATCGAAAGGTGCTGGCCTGA

Upstream 100 bases:

>100_bases
CGTGGGGTCTATGCCCGAGCGTTGCGGGAATTAGCGCCGGCTCTGAAGAAAGGAAAGCGTACTCCTCATACTAATCAGAC
TGTGCCTGGAGACTCACCCG

Downstream 100 bases:

>100_bases
TGCGAACGCTATGCATATCGACCAATTAAATAAAACTAATCGAGAGAGCGTCATGACCGGCACCTCTCGTTGCGCTTTAT
CGATAGATGGGCACGCTCCT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 757; Mature: 757

Protein sequence:

>757_residues
MLDTLRRIVQEVNNAADIPSALDLMAKRVRDAMGTEVCSIYLRDEEEQRYMLMASEGLKREAVGKVSLGLSEGLVGQVGL
REEPINLEDAFTHPKFHYLAGTGEDAFHAFLGVPVMHHGKVLGIMVVQQRDVRRFDQSEEAFLVTISAQLSAVIAHAHAS
GVLFDQLGGGADLPSFYDGLPGCPGAAIGYGVLLFPEADLASVPDRDVEDVSEEISRLDDALVRVRQEIRDLAERASRSL
GAEEQALFDVYLRMLDRHALPAEVIAAIREGQWAQGALRTVIDVHVHNFEMMDDPYLRERAADVRDLGRRVLAQLQSQTR
RHIIFPDECILLGEDISAPMLMEVPRERVRGIVTTRGSRNSHMAIVARAMGIPTVVGAQNLPLKKMDDKEIIVDGFRGRV
VANASAELKQQFQEIIAEEESLQAGLEKLRDEPAQTLDGTRITLQVNTGLMTDINRSLERGAEGVGLYRTEIPFMVRDRF
PSEEEQRVIYREQLAAFAPHSVTMRTLDIGGDKSLSYFPIEDENPFLGWRGIRVTLDHPEIFMVQVRAMIKASEGLDNLR
IMLPMITSVIEAEEAQHLIHRAWLEVRDEGLDVSMPPVGVMIEVPASVYQARALAQRVDFLSVGTNDLTQYLLAVDRNNR
QVASLYNSYHPAVLHALMHVVEQAREEHTPVSVCGEMAGEPSAALLLMAMGVDVLSMNASNLLKVKAAIRQVDISFVRRL
LNEVLAMDNGEVIGSYVDLQLTKAGLGELLRNRKVLA

Sequences:

>Translated_757_residues
MLDTLRRIVQEVNNAADIPSALDLMAKRVRDAMGTEVCSIYLRDEEEQRYMLMASEGLKREAVGKVSLGLSEGLVGQVGL
REEPINLEDAFTHPKFHYLAGTGEDAFHAFLGVPVMHHGKVLGIMVVQQRDVRRFDQSEEAFLVTISAQLSAVIAHAHAS
GVLFDQLGGGADLPSFYDGLPGCPGAAIGYGVLLFPEADLASVPDRDVEDVSEEISRLDDALVRVRQEIRDLAERASRSL
GAEEQALFDVYLRMLDRHALPAEVIAAIREGQWAQGALRTVIDVHVHNFEMMDDPYLRERAADVRDLGRRVLAQLQSQTR
RHIIFPDECILLGEDISAPMLMEVPRERVRGIVTTRGSRNSHMAIVARAMGIPTVVGAQNLPLKKMDDKEIIVDGFRGRV
VANASAELKQQFQEIIAEEESLQAGLEKLRDEPAQTLDGTRITLQVNTGLMTDINRSLERGAEGVGLYRTEIPFMVRDRF
PSEEEQRVIYREQLAAFAPHSVTMRTLDIGGDKSLSYFPIEDENPFLGWRGIRVTLDHPEIFMVQVRAMIKASEGLDNLR
IMLPMITSVIEAEEAQHLIHRAWLEVRDEGLDVSMPPVGVMIEVPASVYQARALAQRVDFLSVGTNDLTQYLLAVDRNNR
QVASLYNSYHPAVLHALMHVVEQAREEHTPVSVCGEMAGEPSAALLLMAMGVDVLSMNASNLLKVKAAIRQVDISFVRRL
LNEVLAMDNGEVIGSYVDLQLTKAGLGELLRNRKVLA
>Mature_757_residues
MLDTLRRIVQEVNNAADIPSALDLMAKRVRDAMGTEVCSIYLRDEEEQRYMLMASEGLKREAVGKVSLGLSEGLVGQVGL
REEPINLEDAFTHPKFHYLAGTGEDAFHAFLGVPVMHHGKVLGIMVVQQRDVRRFDQSEEAFLVTISAQLSAVIAHAHAS
GVLFDQLGGGADLPSFYDGLPGCPGAAIGYGVLLFPEADLASVPDRDVEDVSEEISRLDDALVRVRQEIRDLAERASRSL
GAEEQALFDVYLRMLDRHALPAEVIAAIREGQWAQGALRTVIDVHVHNFEMMDDPYLRERAADVRDLGRRVLAQLQSQTR
RHIIFPDECILLGEDISAPMLMEVPRERVRGIVTTRGSRNSHMAIVARAMGIPTVVGAQNLPLKKMDDKEIIVDGFRGRV
VANASAELKQQFQEIIAEEESLQAGLEKLRDEPAQTLDGTRITLQVNTGLMTDINRSLERGAEGVGLYRTEIPFMVRDRF
PSEEEQRVIYREQLAAFAPHSVTMRTLDIGGDKSLSYFPIEDENPFLGWRGIRVTLDHPEIFMVQVRAMIKASEGLDNLR
IMLPMITSVIEAEEAQHLIHRAWLEVRDEGLDVSMPPVGVMIEVPASVYQARALAQRVDFLSVGTNDLTQYLLAVDRNNR
QVASLYNSYHPAVLHALMHVVEQAREEHTPVSVCGEMAGEPSAALLLMAMGVDVLSMNASNLLKVKAAIRQVDISFVRRL
LNEVLAMDNGEVIGSYVDLQLTKAGLGELLRNRKVLA

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=755, Percent_Identity=41.1920529801325, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1788756, Length=562, Percent_Identity=37.3665480427046, Blast_Score=349, Evalue=5e-97,
Organism=Escherichia coli, GI48994992, Length=558, Percent_Identity=30.8243727598566, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1788726, Length=429, Percent_Identity=33.1002331002331, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1787994, Length=407, Percent_Identity=26.2899262899263, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 84016; Mature: 84016

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTLRRIVQEVNNAADIPSALDLMAKRVRDAMGTEVCSIYLRDEEEQRYMLMASEGLKR
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEHHCCCCH
EAVGKVSLGLSEGLVGQVGLREEPINLEDAFTHPKFHYLAGTGEDAFHAFLGVPVMHHGK
HHHCHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHCCCHHHCCC
VLGIMVVQQRDVRRFDQSEEAFLVTISAQLSAVIAHAHASGVLFDQLGGGADLPSFYDGL
EEEEEEECHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCCC
PGCPGAAIGYGVLLFPEADLASVPDRDVEDVSEEISRLDDALVRVRQEIRDLAERASRSL
CCCCCHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAEEQALFDVYLRMLDRHALPAEVIAAIREGQWAQGALRTVIDVHVHNFEMMDDPYLRER
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCHHHHHH
AADVRDLGRRVLAQLQSQTRRHIIFPDECILLGEDISAPMLMEVPRERVRGIVTTRGSRN
HHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCHHHHCCHHHHCEEEEECCCCC
SHMAIVARAMGIPTVVGAQNLPLKKMDDKEIIVDGFRGRVVANASAELKQQFQEIIAEEE
CCHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHH
SLQAGLEKLRDEPAQTLDGTRITLQVNTGLMTDINRSLERGAEGVGLYRTEIPFMVRDRF
HHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCEECCC
PSEEEQRVIYREQLAAFAPHSVTMRTLDIGGDKSLSYFPIEDENPFLGWRGIRVTLDHPE
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCEEEEEEEEEECCCC
IFMVQVRAMIKASEGLDNLRIMLPMITSVIEAEEAQHLIHRAWLEVRDEGLDVSMPPVGV
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
MIEVPASVYQARALAQRVDFLSVGTNDLTQYLLAVDRNNRQVASLYNSYHPAVLHALMHV
EEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
VEQAREEHTPVSVCGEMAGEPSAALLLMAMGVDVLSMNASNLLKVKAAIRQVDISFVRRL
HHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHHHHHHH
LNEVLAMDNGEVIGSYVDLQLTKAGLGELLRNRKVLA
HHHHHCCCCCCEEEEEEEEEEHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLDTLRRIVQEVNNAADIPSALDLMAKRVRDAMGTEVCSIYLRDEEEQRYMLMASEGLKR
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEHHCCCCH
EAVGKVSLGLSEGLVGQVGLREEPINLEDAFTHPKFHYLAGTGEDAFHAFLGVPVMHHGK
HHHCHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHCCCHHHCCC
VLGIMVVQQRDVRRFDQSEEAFLVTISAQLSAVIAHAHASGVLFDQLGGGADLPSFYDGL
EEEEEEECHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCCC
PGCPGAAIGYGVLLFPEADLASVPDRDVEDVSEEISRLDDALVRVRQEIRDLAERASRSL
CCCCCHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAEEQALFDVYLRMLDRHALPAEVIAAIREGQWAQGALRTVIDVHVHNFEMMDDPYLRER
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCHHHHHH
AADVRDLGRRVLAQLQSQTRRHIIFPDECILLGEDISAPMLMEVPRERVRGIVTTRGSRN
HHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCHHHHCCHHHHCEEEEECCCCC
SHMAIVARAMGIPTVVGAQNLPLKKMDDKEIIVDGFRGRVVANASAELKQQFQEIIAEEE
CCHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHH
SLQAGLEKLRDEPAQTLDGTRITLQVNTGLMTDINRSLERGAEGVGLYRTEIPFMVRDRF
HHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCEECCC
PSEEEQRVIYREQLAAFAPHSVTMRTLDIGGDKSLSYFPIEDENPFLGWRGIRVTLDHPE
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCEEEEEEEEEECCCC
IFMVQVRAMIKASEGLDNLRIMLPMITSVIEAEEAQHLIHRAWLEVRDEGLDVSMPPVGV
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
MIEVPASVYQARALAQRVDFLSVGTNDLTQYLLAVDRNNRQVASLYNSYHPAVLHALMHV
EEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
VEQAREEHTPVSVCGEMAGEPSAALLLMAMGVDVLSMNASNLLKVKAAIRQVDISFVRRL
HHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHHHHHHH
LNEVLAMDNGEVIGSYVDLQLTKAGLGELLRNRKVLA
HHHHHCCCCCCEEEEEEEEEEHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]