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The map label for this gene is thyA

Identifier: 110835205

GI number: 110835205

Start: 2667965

End: 2668798

Strand: Reverse

Name: thyA

Synonym: ABO_2344

Alternate gene names: 110835205

Gene position: 2668798-2667965 (Counterclockwise)

Preceding gene: 110835206

Following gene: 110835204

Centisome position: 85.53

GC content: 56.35

Gene sequence:

>834_bases
ATGAAGCAATACCTTGATCTGCTTCGCCACGTCCGCGAGCACGGAACGTATAAGGAAGACCGCACTGGCACCGGCACCTA
TGCGGTGTTTGGTTACCAGATGCGCTATGACCTGAGCCAGGGCTTCCCTATGCTCACCACCAAGAAGCTGCATCTGCGCT
CGATTATTCACGAGTTACTGTGGTTCCTGTCTGGTGACACCAATATCCGTTATCTAAAGGAAAACGGCGTGTCCATCTGG
GATGGCTGGGCGACACCGGAGGGTGAGCTGGGGCCTGTCTATGGTGAGCAGTGGCGCAGTTGGAAAACGGCGGATGGCGG
GGTGATTGATCAGATCACCGAGGTGCTGAAAGAGATCAAGCGCAATCCGGACTCCCGGCGCCTGGTGGTCAGCGCTTGGA
ACCCCGCGGTGCTGCCGGATCCGTCCATTTCACCGGATGCCAATGCAGCAGCAGGCAAGCAAGCGTTGCCACCCTGCCAC
TGCTTGTTCCAGTTCTATGTGGCGGACGGCAAACTTAGTTGCCAGCTATATCAACGCAGTGGCGATATTTTTCTTGGCGT
GCCATTCAATATTGCGTCCTATGCCTTGCTGACGCTGATGATGGCGCAGGTGTGTGATCTGCAGCCGGGTGATTTTGTGC
ATACCTTGGGCGATGCGCATCTCTACTCCAACCATCTGGAGCAGGCGGATACTCAACTGGCGCGTACGCCTGGCCCGTTG
CCGACAATGACGTTGAACCCGGCGGTGAAGGATCTGTTTGCCTTCACCTTTGACGATTTCACCGTCAGCAACTACAACCC
CGACGCGCACATCAAAGCGCCGGTGGCAATCTAA

Upstream 100 bases:

>100_bases
GCTGGTTGACCATGGGCATGCTGTTGAGCATCCCGATGGTTGTGCTCGGTATCGCTGTGTTGGTGTGGGCATATCGAAAC
GATGAGCGAGGAGTGAAGGC

Downstream 100 bases:

>100_bases
CGACTCCTCCCTGAAGCCGGATGCTCGATGCAACAGAGCTTTTGTCCGGCGTTAGGGGGTGGGTTCAAGCGTAGGTATGT
TCATGTCCATTCGACTTTCC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKQYLDLLRHVREHGTYKEDRTGTGTYAVFGYQMRYDLSQGFPMLTTKKLHLRSIIHELLWFLSGDTNIRYLKENGVSIW
DGWATPEGELGPVYGEQWRSWKTADGGVIDQITEVLKEIKRNPDSRRLVVSAWNPAVLPDPSISPDANAAAGKQALPPCH
CLFQFYVADGKLSCQLYQRSGDIFLGVPFNIASYALLTLMMAQVCDLQPGDFVHTLGDAHLYSNHLEQADTQLARTPGPL
PTMTLNPAVKDLFAFTFDDFTVSNYNPDAHIKAPVAI

Sequences:

>Translated_277_residues
MKQYLDLLRHVREHGTYKEDRTGTGTYAVFGYQMRYDLSQGFPMLTTKKLHLRSIIHELLWFLSGDTNIRYLKENGVSIW
DGWATPEGELGPVYGEQWRSWKTADGGVIDQITEVLKEIKRNPDSRRLVVSAWNPAVLPDPSISPDANAAAGKQALPPCH
CLFQFYVADGKLSCQLYQRSGDIFLGVPFNIASYALLTLMMAQVCDLQPGDFVHTLGDAHLYSNHLEQADTQLARTPGPL
PTMTLNPAVKDLFAFTFDDFTVSNYNPDAHIKAPVAI
>Mature_277_residues
MKQYLDLLRHVREHGTYKEDRTGTGTYAVFGYQMRYDLSQGFPMLTTKKLHLRSIIHELLWFLSGDTNIRYLKENGVSIW
DGWATPEGELGPVYGEQWRSWKTADGGVIDQITEVLKEIKRNPDSRRLVVSAWNPAVLPDPSISPDANAAAGKQALPPCH
CLFQFYVADGKLSCQLYQRSGDIFLGVPFNIASYALLTLMMAQVCDLQPGDFVHTLGDAHLYSNHLEQADTQLARTPGPL
PTMTLNPAVKDLFAFTFDDFTVSNYNPDAHIKAPVAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=295, Percent_Identity=47.4576271186441, Blast_Score=273, Evalue=1e-73,
Organism=Escherichia coli, GI1789191, Length=277, Percent_Identity=61.0108303249097, Blast_Score=355, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71993377, Length=294, Percent_Identity=45.2380952380952, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324648, Length=305, Percent_Identity=38.6885245901639, Blast_Score=199, Evalue=4e-52,
Organism=Drosophila melanogaster, GI17137556, Length=297, Percent_Identity=48.1481481481481, Blast_Score=284, Evalue=5e-77,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_ALCBS (Q0VM06)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_694064.1
- ProteinModelPortal:   Q0VM06
- SMR:   Q0VM06
- STRING:   Q0VM06
- GeneID:   4211942
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2344
- NMPDR:   fig|393595.12.peg.2345
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q0VM06
- BioCyc:   ABOR393595:ABO_2344-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 31050; Mature: 31050

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLLRHVREHGTYKEDRTGTGTYAVFGYQMRYDLSQGFPMLTTKKLHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEHHCCCCCEEHHHHHHHHHHHHHH
WFLSGDTNIRYLKENGVSIWDGWATPEGELGPVYGEQWRSWKTADGGVIDQITEVLKEIK
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHH
RNPDSRRLVVSAWNPAVLPDPSISPDANAAAGKQALPPCHCLFQFYVADGKLSCQLYQRS
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECC
GDIFLGVPFNIASYALLTLMMAQVCDLQPGDFVHTLGDAHLYSNHLEQADTQLARTPGPL
CCEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
PTMTLNPAVKDLFAFTFDDFTVSNYNPDAHIKAPVAI
CCEEECHHHHHHHHHHHCCEEECCCCCCCEEECCCCC
>Mature Secondary Structure
MKQYLDLLRHVREHGTYKEDRTGTGTYAVFGYQMRYDLSQGFPMLTTKKLHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEHHCCCCCEEHHHHHHHHHHHHHH
WFLSGDTNIRYLKENGVSIWDGWATPEGELGPVYGEQWRSWKTADGGVIDQITEVLKEIK
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHH
RNPDSRRLVVSAWNPAVLPDPSISPDANAAAGKQALPPCHCLFQFYVADGKLSCQLYQRS
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECC
GDIFLGVPFNIASYALLTLMMAQVCDLQPGDFVHTLGDAHLYSNHLEQADTQLARTPGPL
CCEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
PTMTLNPAVKDLFAFTFDDFTVSNYNPDAHIKAPVAI
CCEEECHHHHHHHHHHHCCEEECCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA