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The map label for this gene is queE [H]

Identifier: 110636375

GI number: 110636375

Start: 4358989

End: 4359729

Strand: Reverse

Name: queE [H]

Synonym: Meso_4051

Alternate gene names: 110636375

Gene position: 4359729-4358989 (Counterclockwise)

Preceding gene: 110636376

Following gene: 110636370

Centisome position: 98.81

GC content: 60.46

Gene sequence:

>741_bases
ATGACCAGCGACAAATCCGAAACGCATATCCGGGTGGCGGAAATCTTCGGGCCGACCATTCAGGGCGAGGGGCCCCTGAT
CGGTCTGCCGACCGTCTTTGTGAGGACGGGCGGATGCGACTACCGGTGCGCTTGGTGCGACAGCCTGCATGCGGTCGACA
CCCAATACCGGCACGATTGGGAGCCTATGACGGCAAAGGCTGTTTGGGAGAAAGTGCAGGTGCTTTCGGCCAACACTCCG
CTCATGGTGTCGCTTTCCGGCGGCAATCCGGCGATACAGCCGCTGGGCGATCTCATCGCATTGGGCAAATCATCGGGCTA
TCGGTTCGCGCTCGAAACCCAAGGATCGCTGGCGCGCGAGTGGTTTTCCGAGCTCGACGTTCTGATTATCAGTCCCAAGC
CTCCCTCCAGCGGCATGGAAATGGATTGGCAGAGGTTAAGCGACTGCATCGCCCGTGCGGGAGAAGGAACCGAAGTCGCG
CTCAAGATCGTGATCTTCGACGAGGCCGATTATGCCTTTGCGCGCGCGGTTTCGGCCCGTCATGGCGGCTTGCCGCTTTA
TCTTCAGCCTGGCAATCACACTCCGCCGCCAGCCGATGCCCATTACGCGCCGATCGACATTGAGGGCGTGATGCGGCGAA
TGCGCTGGCTAATTAACAAAGTTGCCGCAGATGCGTGGTTTTCAGTCCGGGTTCTGCCCCAGCTGCACGTCCTGGTCTGG
GGCAACAAGCGCGGCGTTTAG

Upstream 100 bases:

>100_bases
GGCTGGCCAAGCACTTTTTCGACTGGTGCCGTGAGAGGCTGCCCGAGACGTCCTGCGTTCGCGTCAGCGAAACGCCCAAG
ACCTGGGCCGAGTACCGGCC

Downstream 100 bases:

>100_bases
GCGGAATGACCAAATATGATTGGCCCATCATGGAACCCTTCATCACCCCCGCTCCGCGAACTTTCTCGCATATTTCCTTT
CCAGCCCGGCATGAGTGGAC

Product: radical SAM family protein

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTSDKSETHIRVAEIFGPTIQGEGPLIGLPTVFVRTGGCDYRCAWCDSLHAVDTQYRHDWEPMTAKAVWEKVQVLSANTP
LMVSLSGGNPAIQPLGDLIALGKSSGYRFALETQGSLAREWFSELDVLIISPKPPSSGMEMDWQRLSDCIARAGEGTEVA
LKIVIFDEADYAFARAVSARHGGLPLYLQPGNHTPPPADAHYAPIDIEGVMRRMRWLINKVAADAWFSVRVLPQLHVLVW
GNKRGV

Sequences:

>Translated_246_residues
MTSDKSETHIRVAEIFGPTIQGEGPLIGLPTVFVRTGGCDYRCAWCDSLHAVDTQYRHDWEPMTAKAVWEKVQVLSANTP
LMVSLSGGNPAIQPLGDLIALGKSSGYRFALETQGSLAREWFSELDVLIISPKPPSSGMEMDWQRLSDCIARAGEGTEVA
LKIVIFDEADYAFARAVSARHGGLPLYLQPGNHTPPPADAHYAPIDIEGVMRRMRWLINKVAADAWFSVRVLPQLHVLVW
GNKRGV
>Mature_245_residues
TSDKSETHIRVAEIFGPTIQGEGPLIGLPTVFVRTGGCDYRCAWCDSLHAVDTQYRHDWEPMTAKAVWEKVQVLSANTPL
MVSLSGGNPAIQPLGDLIALGKSSGYRFALETQGSLAREWFSELDVLIISPKPPSSGMEMDWQRLSDCIARAGEGTEVAL
KIVIFDEADYAFARAVSARHGGLPLYLQPGNHTPPPADAHYAPIDIEGVMRRMRWLINKVAADAWFSVRVLPQLHVLVWG
NKRGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 27079; Mature: 26948

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDKSETHIRVAEIFGPTIQGEGPLIGLPTVFVRTGGCDYRCAWCDSLHAVDTQYRHDW
CCCCCCCCEEEEEHHCCCCCCCCCCEEECCEEEEEECCCCEEEECCCCCHHHCHHHCCCC
EPMTAKAVWEKVQVLSANTPLMVSLSGGNPAIQPLGDLIALGKSSGYRFALETQGSLARE
CCCHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHH
WFSELDVLIISPKPPSSGMEMDWQRLSDCIARAGEGTEVALKIVIFDEADYAFARAVSAR
HHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHC
HGGLPLYLQPGNHTPPPADAHYAPIDIEGVMRRMRWLINKVAADAWFSVRVLPQLHVLVW
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEE
GNKRGV
CCCCCC
>Mature Secondary Structure 
TSDKSETHIRVAEIFGPTIQGEGPLIGLPTVFVRTGGCDYRCAWCDSLHAVDTQYRHDW
CCCCCCCEEEEEHHCCCCCCCCCCEEECCEEEEEECCCCEEEECCCCCHHHCHHHCCCC
EPMTAKAVWEKVQVLSANTPLMVSLSGGNPAIQPLGDLIALGKSSGYRFALETQGSLARE
CCCHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHH
WFSELDVLIISPKPPSSGMEMDWQRLSDCIARAGEGTEVALKIVIFDEADYAFARAVSAR
HHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHC
HGGLPLYLQPGNHTPPPADAHYAPIDIEGVMRRMRWLINKVAADAWFSVRVLPQLHVLVW
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEE
GNKRGV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]