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The map label for this gene is gsiB [H]

Identifier: 110636062

GI number: 110636062

Start: 4036002

End: 4037555

Strand: Reverse

Name: gsiB [H]

Synonym: Meso_3737

Alternate gene names: 110636062

Gene position: 4037555-4036002 (Counterclockwise)

Preceding gene: 110636063

Following gene: 110636061

Centisome position: 91.5

GC content: 58.75

Gene sequence:

>1554_bases
ATGAGCTTCTCACGCTGCGACTTTGGCCGGCGAGCATTGCTCCGCCTCGGCCTTGCCTTCTCGATCGCACTGTCGATGCA
GTTCGGGTCACTACAATATGTGGGATCGGCTGCAGCGCAGGACAGCAGCACCGTCAGAATCGCCATCCCGCTTGGGTTTC
GTGGTCTCGATCCCCATGAGGGCGTCAATCCTCGGGCCGAAGTCACACTTCAAAGCAACATCTATTCCAGCCTGACCCGG
ATTACGCCGGAGTTGGAGGTCGTCGGCGACCTCGCGACGCAATGGGAGCAGCCAGAGCCTTTGATATGGGAGTTCGAGCT
GAATCTGGCGGCCCGGTTCGAGAACGGAACGCCGCTGGACGCCGACGAGATCGTGGCGAACTTTTCCCGCATGTTCGACG
CCGAACTGGGCTTTACGTTCGCAGCCGATCTGAAGCCGGTCATCAAGAGCGTCGAAGCGGTGACGCCGACCCGCGTGCGC
TTTACGCTTTCAAGCCCGTATCCGGACTTCCTGCGCCGGATGTCCTATATCTACTTCCTCGATACGGAATGGGCGAATAG
CCACAACCCCAAGCTGGAGGCGAATGCCTCCGGTCCTTACAGGCTGGTCAGTTTTGATCCGGAAGCGGGGGCGGTCCTCG
AAGCCAACCCCGACTACTACGGAGAACAACCCGCTTTCAGCAACGCCGAGATGAAGGTGCTGCGCTCGCCGGCGCAGCGG
CTGACCGGGCTTCTGGCCGGCGAGGTCGACGTCGCCACCATCCTTGATCCACAGGATCTGAAGCAGATCGAGGCAACTGG
CGAGTTCAATGTAGGCGCCATCGACAGTTCGCGCAGCGTCTATCTCTGGTACAACACCACTAAAGAACCGTTCGATAATA
AGCTGGTTCGTCAGGCACTCAACTACGCAGTCAACGTTCCGCAGATCACGGAAACGCTGTTGAGCGGCTATGGCAAGGCG
CTTGAAGGTCAAGTGTTTAGCCCGATCTATACGGGCTACAATCCCGATCTCAAAGCCTATCCCTATGATCCGGAGAAGGC
AAAGGCTCTCCTGGCCGAGGCTGGATACCCTAACGGGTTCACGACGACGATGAGCGTGCCGACCGGCGCCTATGTGGCGG
GCGATCAGATCGCCCAGGTTGTCGCCGCGCAGCTCGCCCAGATCGGAGTGAAGGTCAATATCGAGCAGATGCCGATGCAG
ACCATGACGCAGCAGGGAAACAACCCGGAAACCGCAGCGCCGATGCGTTTTGTAGGCTATGCGGCCTACGACATGTCGGC
TCGAGGCTTTCTGATCTATTTCCGCTCGGCGGGAGCGTCGAACCAAGCGAAGGATCCAGGCTACGACAAGCTCTATGAAG
CCTATCTGAACGCTGAGACAGACGAGGAAAAGGCCAAACTGGTGGCCGAGATCACCGAATATATGCGTGATTTTGCGCCG
ATGCTGTTTCTCTATTCACAACCGACGACCTATGCCGTGCGCAACAGCGTCGAATGGACCCCGCGTCCAGACGACTGGCT
GCGCGCCTTCGACATGACTCCGGTCGCGAATTGA

Upstream 100 bases:

>100_bases
CGGTCGCGTGCCATTACCCCGTCAGTCGCTCCCAAACGCACAAAAAGAAGCTGGAGAATCAACAAACCAGCTCGCCGTAT
AAAGAGGAAAGGAACATGAA

Downstream 100 bases:

>100_bases
AAGCCCTGGACGAGGCCAAGCCGCTTGGCTTGGCCTTCGTTGACCATCCCCTTTGAAGGACGCCATGAATCTGCAAGATG
ATCTGTTCCTCGATGCCGAG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSFSRCDFGRRALLRLGLAFSIALSMQFGSLQYVGSAAAQDSSTVRIAIPLGFRGLDPHEGVNPRAEVTLQSNIYSSLTR
ITPELEVVGDLATQWEQPEPLIWEFELNLAARFENGTPLDADEIVANFSRMFDAELGFTFAADLKPVIKSVEAVTPTRVR
FTLSSPYPDFLRRMSYIYFLDTEWANSHNPKLEANASGPYRLVSFDPEAGAVLEANPDYYGEQPAFSNAEMKVLRSPAQR
LTGLLAGEVDVATILDPQDLKQIEATGEFNVGAIDSSRSVYLWYNTTKEPFDNKLVRQALNYAVNVPQITETLLSGYGKA
LEGQVFSPIYTGYNPDLKAYPYDPEKAKALLAEAGYPNGFTTTMSVPTGAYVAGDQIAQVVAAQLAQIGVKVNIEQMPMQ
TMTQQGNNPETAAPMRFVGYAAYDMSARGFLIYFRSAGASNQAKDPGYDKLYEAYLNAETDEEKAKLVAEITEYMRDFAP
MLFLYSQPTTYAVRNSVEWTPRPDDWLRAFDMTPVAN

Sequences:

>Translated_517_residues
MSFSRCDFGRRALLRLGLAFSIALSMQFGSLQYVGSAAAQDSSTVRIAIPLGFRGLDPHEGVNPRAEVTLQSNIYSSLTR
ITPELEVVGDLATQWEQPEPLIWEFELNLAARFENGTPLDADEIVANFSRMFDAELGFTFAADLKPVIKSVEAVTPTRVR
FTLSSPYPDFLRRMSYIYFLDTEWANSHNPKLEANASGPYRLVSFDPEAGAVLEANPDYYGEQPAFSNAEMKVLRSPAQR
LTGLLAGEVDVATILDPQDLKQIEATGEFNVGAIDSSRSVYLWYNTTKEPFDNKLVRQALNYAVNVPQITETLLSGYGKA
LEGQVFSPIYTGYNPDLKAYPYDPEKAKALLAEAGYPNGFTTTMSVPTGAYVAGDQIAQVVAAQLAQIGVKVNIEQMPMQ
TMTQQGNNPETAAPMRFVGYAAYDMSARGFLIYFRSAGASNQAKDPGYDKLYEAYLNAETDEEKAKLVAEITEYMRDFAP
MLFLYSQPTTYAVRNSVEWTPRPDDWLRAFDMTPVAN
>Mature_516_residues
SFSRCDFGRRALLRLGLAFSIALSMQFGSLQYVGSAAAQDSSTVRIAIPLGFRGLDPHEGVNPRAEVTLQSNIYSSLTRI
TPELEVVGDLATQWEQPEPLIWEFELNLAARFENGTPLDADEIVANFSRMFDAELGFTFAADLKPVIKSVEAVTPTRVRF
TLSSPYPDFLRRMSYIYFLDTEWANSHNPKLEANASGPYRLVSFDPEAGAVLEANPDYYGEQPAFSNAEMKVLRSPAQRL
TGLLAGEVDVATILDPQDLKQIEATGEFNVGAIDSSRSVYLWYNTTKEPFDNKLVRQALNYAVNVPQITETLLSGYGKAL
EGQVFSPIYTGYNPDLKAYPYDPEKAKALLAEAGYPNGFTTTMSVPTGAYVAGDQIAQVVAAQLAQIGVKVNIEQMPMQT
MTQQGNNPETAAPMRFVGYAAYDMSARGFLIYFRSAGASNQAKDPGYDKLYEAYLNAETDEEKAKLVAEITEYMRDFAPM
LFLYSQPTTYAVRNSVEWTPRPDDWLRAFDMTPVAN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=396, Percent_Identity=27.7777777777778, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789966, Length=539, Percent_Identity=24.30426716141, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1787762, Length=431, Percent_Identity=26.4501160092807, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789887, Length=384, Percent_Identity=26.5625, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787551, Length=354, Percent_Identity=24.0112994350282, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57315; Mature: 57184

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSRCDFGRRALLRLGLAFSIALSMQFGSLQYVGSAAAQDSSTVRIAIPLGFRGLDPHE
CCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
GVNPRAEVTLQSNIYSSLTRITPELEVVGDLATQWEQPEPLIWEFELNLAARFENGTPLD
CCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCCC
ADEIVANFSRMFDAELGFTFAADLKPVIKSVEAVTPTRVRFTLSSPYPDFLRRMSYIYFL
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCEEEEEE
DTEWANSHNPKLEANASGPYRLVSFDPEAGAVLEANPDYYGEQPAFSNAEMKVLRSPAQR
ECCCCCCCCCEEEECCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
LTGLLAGEVDVATILDPQDLKQIEATGEFNVGAIDSSRSVYLWYNTTKEPFDNKLVRQAL
HHHHHCCCCEEEEECCHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHH
NYAVNVPQITETLLSGYGKALEGQVFSPIYTGYNPDLKAYPYDPEKAKALLAEAGYPNGF
HHCCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
TTTMSVPTGAYVAGDQIAQVVAAQLAQIGVKVNIEQMPMQTMTQQGNNPETAAPMRFVGY
EEEEECCCCCEECHHHHHHHHHHHHHHCCCEEEHHHCCHHHHHHCCCCCCCCCCHHHEEE
AAYDMSARGFLIYFRSAGASNQAKDPGYDKLYEAYLNAETDEEKAKLVAEITEYMRDFAP
EEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MLFLYSQPTTYAVRNSVEWTPRPDDWLRAFDMTPVAN
EEEEECCCCEEEECCCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
SFSRCDFGRRALLRLGLAFSIALSMQFGSLQYVGSAAAQDSSTVRIAIPLGFRGLDPHE
CCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
GVNPRAEVTLQSNIYSSLTRITPELEVVGDLATQWEQPEPLIWEFELNLAARFENGTPLD
CCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCCC
ADEIVANFSRMFDAELGFTFAADLKPVIKSVEAVTPTRVRFTLSSPYPDFLRRMSYIYFL
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCEEEEEE
DTEWANSHNPKLEANASGPYRLVSFDPEAGAVLEANPDYYGEQPAFSNAEMKVLRSPAQR
ECCCCCCCCCEEEECCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
LTGLLAGEVDVATILDPQDLKQIEATGEFNVGAIDSSRSVYLWYNTTKEPFDNKLVRQAL
HHHHHCCCCEEEEECCHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHH
NYAVNVPQITETLLSGYGKALEGQVFSPIYTGYNPDLKAYPYDPEKAKALLAEAGYPNGF
HHCCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
TTTMSVPTGAYVAGDQIAQVVAAQLAQIGVKVNIEQMPMQTMTQQGNNPETAAPMRFVGY
EEEEECCCCCEECHHHHHHHHHHHHHHCCCEEEHHHCCHHHHHHCCCCCCCCCCHHHEEE
AAYDMSARGFLIYFRSAGASNQAKDPGYDKLYEAYLNAETDEEKAKLVAEITEYMRDFAP
EEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MLFLYSQPTTYAVRNSVEWTPRPDDWLRAFDMTPVAN
EEEEECCCCEEEECCCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA