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The map label for this gene is mtnN [C]

Identifier: 110632854

GI number: 110632854

Start: 548491

End: 549156

Strand: Direct

Name: mtnN [C]

Synonym: Meso_0494

Alternate gene names: 110632854

Gene position: 548491-549156 (Clockwise)

Preceding gene: 110632853

Following gene: 110632855

Centisome position: 12.43

GC content: 59.91

Gene sequence:

>666_bases
GTGAGGGAAGTTCCGCTCACAAGGCTCGGGGGCCAGCGCGTGCTTTTCGCAATGGCGGCGGAGCCGGAATATGGCGCGCA
TCTCAAAAAGCGTTTCCGGCCGCTCATAACGGGTGTCGGGCCGGTGGAGGCTGCGGTTGAGCTTACGGCTGCGCTTTCAC
AGCTCAAATCCGAAGGCGAAGCGCCGCAACTGGTTGTCTCGCTCGGCTCCGCCGGCAGCCGCATGCTCGAGCAAACCGAG
ATCTATCAGGCAAGCTCCGTCTCCTACCGGGACATGGATGCTTCTCCCTTGGGCTTCGAAAAAGGAGTAACGCCCTTCCT
AGGCCTTCCGAAGGAGATGCCGCTGCCTTTCCGCATTCCGGGGATCAAGACGGCTACCCTTTCGACGGGCGGCGGTATCG
TCAGCGGCGGTGCGTATGACACCATCGCTGCGGACATGGTCGACATGGAAACATATGCCTGCCTGCGCGCCTGTCAGCAT
TTCGGCGTGCCGCTAGTAGCACTTCGCGGCATTTCCGATGGCGCGGAAGAACTTCGTCATGTCGGTAATTGGACGCAATA
TCTGCACATTATAGACGAGAAGCTCGCAGCTGCGGTGGACCGGCTGGATACAGCGATCTGCGATGGCGATTTGCTATGCT
GCTCGAGAAATCCCGATTCCGCCTGA

Upstream 100 bases:

>100_bases
ACGGTCAGGCCTACGCCTATGACGGGGACGGAGAGGCAAAGCTGATCGCCACGATGACCGGCACCATGATGACGATCGTT
GGTCGGCAGGAGATTGCGGG

Downstream 100 bases:

>100_bases
TCGGATCTGATCGGGTGGCGGAAAACGCATGGTGCTGAACGGATTGCGGCAAAAAACCCCTATCGAACCCGTTGCGCGGG
ATCGCTTCCTTCTTTAAAGC

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: 5-methylthio-D-ribose; adenine

Alternate protein names: Nucleosidase; Nucleoside Phosphorylase; MTA/SAH Nucleosidase; Nucleoside Phosphorylase-Like Protein; Phosphorylase; 5-Methylthioadenosine/S-Adenosylhomocysteinenucl Eosidase

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MREVPLTRLGGQRVLFAMAAEPEYGAHLKKRFRPLITGVGPVEAAVELTAALSQLKSEGEAPQLVVSLGSAGSRMLEQTE
IYQASSVSYRDMDASPLGFEKGVTPFLGLPKEMPLPFRIPGIKTATLSTGGGIVSGGAYDTIAADMVDMETYACLRACQH
FGVPLVALRGISDGAEELRHVGNWTQYLHIIDEKLAAAVDRLDTAICDGDLLCCSRNPDSA

Sequences:

>Translated_221_residues
MREVPLTRLGGQRVLFAMAAEPEYGAHLKKRFRPLITGVGPVEAAVELTAALSQLKSEGEAPQLVVSLGSAGSRMLEQTE
IYQASSVSYRDMDASPLGFEKGVTPFLGLPKEMPLPFRIPGIKTATLSTGGGIVSGGAYDTIAADMVDMETYACLRACQH
FGVPLVALRGISDGAEELRHVGNWTQYLHIIDEKLAAAVDRLDTAICDGDLLCCSRNPDSA
>Mature_221_residues
MREVPLTRLGGQRVLFAMAAEPEYGAHLKKRFRPLITGVGPVEAAVELTAALSQLKSEGEAPQLVVSLGSAGSRMLEQTE
IYQASSVSYRDMDASPLGFEKGVTPFLGLPKEMPLPFRIPGIKTATLSTGGGIVSGGAYDTIAADMVDMETYACLRACQH
FGVPLVALRGISDGAEELRHVGNWTQYLHIIDEKLAAAVDRLDTAICDGDLLCCSRNPDSA

Specific function: Responsible For Cleavage Of The Glycosidic Bond In Both 5'-Methylthioadenosine (Mta) And S-Adenosylhomocysteine (Sah). [C]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.2.16; 3.2.2.9

Molecular weight: Translated: 23594; Mature: 23594

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVPLTRLGGQRVLFAMAAEPEYGAHLKKRFRPLITGVGPVEAAVELTAALSQLKSEGE
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
APQLVVSLGSAGSRMLEQTEIYQASSVSYRDMDASPLGFEKGVTPFLGLPKEMPLPFRIP
CCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCHHHCCCCCCCCCEECC
GIKTATLSTGGGIVSGGAYDTIAADMVDMETYACLRACQHFGVPLVALRGISDGAEELRH
CCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
VGNWTQYLHIIDEKLAAAVDRLDTAICDGDLLCCSRNPDSA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
>Mature Secondary Structure
MREVPLTRLGGQRVLFAMAAEPEYGAHLKKRFRPLITGVGPVEAAVELTAALSQLKSEGE
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
APQLVVSLGSAGSRMLEQTEIYQASSVSYRDMDASPLGFEKGVTPFLGLPKEMPLPFRIP
CCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCHHHCCCCCCCCCEECC
GIKTATLSTGGGIVSGGAYDTIAADMVDMETYACLRACQHFGVPLVALRGISDGAEELRH
CCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
VGNWTQYLHIIDEKLAAAVDRLDTAICDGDLLCCSRNPDSA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1.8 {5'-methylthioadenosine}} 0.0004 {5'-methylthioadenosine}} 0.00031 {5'-methylthioadenosine}} 3 {S-adenosylhomocysteine}} 0.0043 {S-adenosylhomocysteine}} [C]

Substrates: methylthioadenosine; H2O

Specific reaction: methylthioadenosine + H2O = 5-methylthio-D-ribose + adenine

General reaction: N-Glycosyl bond hydrolysis [C]

Inhibitor: 5'-Chlor of ormycin; 5'-Ethyl thioadenosine; 5'-Methyl thi of ormycin; 5'-Methyl thioinosine; 5'-Methyl thiotubercidin; 5'-n-Propyl thioadenosine; Adenine; AMP; L-Methionine; N-2-Hydroxy ethyl piperazine-N'-2-ethanesulfonic acid; S-8-Aza-Adenosyl homocyst

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA