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The map label for this gene is waaA [H]

Identifier: 110632799

GI number: 110632799

Start: 484421

End: 485740

Strand: Direct

Name: waaA [H]

Synonym: Meso_0438

Alternate gene names: 110632799

Gene position: 484421-485740 (Clockwise)

Preceding gene: 110632798

Following gene: 110632800

Centisome position: 10.98

GC content: 64.32

Gene sequence:

>1320_bases
ATGAGCGAGCGTTGGGCACGCGCCATGCTGACGACCTATCGCTGGGCGGGCGCGGCCGCCTTCCCCTTCGTCGGCGGCTA
TGTCGCCTGGCGTGTCAGCAAGGGCAAGGAGGACCGGGCCCGGCGGCACGAGCGTTACGGCAGGTTCGATATCCCCCGCC
CGGAGGGCCCGCTGGTCTGGATGCACGCCGCCAGCGTAGGCGAGACCATGGCCGTGATGGGGCTGATCGAGCATTTTCTG
GCGAGCGGCATCAAGGTTGTGCTTACGACCGGCACCGTGACATCGGCGAGCGTGGCTTCGGAGCGGCTCGGAGATCGCAT
CATCCATCAATATGTCCCTCTCGACCTGAAGCCGGCGGTCAGCCGCTTCCTGAACCACTGGAGGCCCGACTTGGCCGTCA
TGGCGGAGTCGGAGATCTGGCCGCTGACGATTCTGGAGTTGGGCGCCCGCCGCGTTCCCCAGGTTCTGGTGAACGGCCGC
ATGTCGGACCGTTCCTTTGCGCGTTGGCAGCGCCGGCCCTCGCTCGCAGAAGTGCTTTTTGAGAACCTGGCCCATGTGGT
GGCGCAGTCGGAACTGGATGGAGCGCGTTTTCGCCAGCTTGGCGCGCGACCCGTTACGGTTTCGGGCAATCTCAAGGTCG
ATACCCATGTGCCGCCCGTCGACCAGAGCGCGCTGGATCTGCTGAGGCGCACAATCAACGGCCGCAAAACCTGGGCTGCC
GTTTCCACCCATGAGGGGGAGGAGATGATTGCGGCCGACGTCCACATGCTCGTGAAAAAGCGGCATCCGGATCTCCTGAC
GATCATCGTTCCGCGCCATCCGGAGCGCTGTGCGGCTCTGAAGGCCGAACTGGCGGCCCGTGGACTGAAGGTTGCCTTGC
GCAACGGAGGTCCTCTGGTTTCGCCATCCACGGATATCCTGCTCGGCAACAGCATCGGCGAGATGGGGCTTTATTTGCGG
CTGACGGAGATCGCCTTCGTCGGCCGCTCCATGACCGGGAAAGGGGGGCAGAACCCGCTGGAACCCGCGATGCTGGAAAC
CGCGGTCCTTTCCGGGGTCAATGTCGAGAACTTCCGGGACTCCTATCGCAGGCTGATCGAACGCGGCGGGGCCAAGCTGG
TGCGCGACTCGCAAATGCTTGCCGGGGCGGTTAATTTTCTTCTCAAGAACGACGAGATGCGTCAGGGGATGATCGCCGCG
GGCAGAAAGACGGTGAAGGAGATGTCGGGCGCACTCACGCGGACTTTGAGCGCGCTCGATCCCTTCATCCATCCGCTGAC
CGTCAAGGCCCGCCTGCAAGGCGGCGATACAGGGGGGTGA

Upstream 100 bases:

>100_bases
AGCGCAAGCGGCAAGAGGTGACGGACGCGCTCAATCTTGCCACGCGCCGCGCCTATGCGCTTGTGGATGGAACCTCCTCC
AACCGCGGCCGTGACACCCG

Downstream 100 bases:

>100_bases
TCTGATGGCCGCAAGCGAAGCGCCGCCCTTCTGGTGGGAGAAGCCGGATTGGCGCGCTTGGGCGCTCTGGCCTTTGTCGT
CGGCTTACGGATTCGCCGCC

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSERWARAMLTTYRWAGAAAFPFVGGYVAWRVSKGKEDRARRHERYGRFDIPRPEGPLVWMHAASVGETMAVMGLIEHFL
ASGIKVVLTTGTVTSASVASERLGDRIIHQYVPLDLKPAVSRFLNHWRPDLAVMAESEIWPLTILELGARRVPQVLVNGR
MSDRSFARWQRRPSLAEVLFENLAHVVAQSELDGARFRQLGARPVTVSGNLKVDTHVPPVDQSALDLLRRTINGRKTWAA
VSTHEGEEMIAADVHMLVKKRHPDLLTIIVPRHPERCAALKAELAARGLKVALRNGGPLVSPSTDILLGNSIGEMGLYLR
LTEIAFVGRSMTGKGGQNPLEPAMLETAVLSGVNVENFRDSYRRLIERGGAKLVRDSQMLAGAVNFLLKNDEMRQGMIAA
GRKTVKEMSGALTRTLSALDPFIHPLTVKARLQGGDTGG

Sequences:

>Translated_439_residues
MSERWARAMLTTYRWAGAAAFPFVGGYVAWRVSKGKEDRARRHERYGRFDIPRPEGPLVWMHAASVGETMAVMGLIEHFL
ASGIKVVLTTGTVTSASVASERLGDRIIHQYVPLDLKPAVSRFLNHWRPDLAVMAESEIWPLTILELGARRVPQVLVNGR
MSDRSFARWQRRPSLAEVLFENLAHVVAQSELDGARFRQLGARPVTVSGNLKVDTHVPPVDQSALDLLRRTINGRKTWAA
VSTHEGEEMIAADVHMLVKKRHPDLLTIIVPRHPERCAALKAELAARGLKVALRNGGPLVSPSTDILLGNSIGEMGLYLR
LTEIAFVGRSMTGKGGQNPLEPAMLETAVLSGVNVENFRDSYRRLIERGGAKLVRDSQMLAGAVNFLLKNDEMRQGMIAA
GRKTVKEMSGALTRTLSALDPFIHPLTVKARLQGGDTGG
>Mature_438_residues
SERWARAMLTTYRWAGAAAFPFVGGYVAWRVSKGKEDRARRHERYGRFDIPRPEGPLVWMHAASVGETMAVMGLIEHFLA
SGIKVVLTTGTVTSASVASERLGDRIIHQYVPLDLKPAVSRFLNHWRPDLAVMAESEIWPLTILELGARRVPQVLVNGRM
SDRSFARWQRRPSLAEVLFENLAHVVAQSELDGARFRQLGARPVTVSGNLKVDTHVPPVDQSALDLLRRTINGRKTWAAV
STHEGEEMIAADVHMLVKKRHPDLLTIIVPRHPERCAALKAELAARGLKVALRNGGPLVSPSTDILLGNSIGEMGLYLRL
TEIAFVGRSMTGKGGQNPLEPAMLETAVLSGVNVENFRDSYRRLIERGGAKLVRDSQMLAGAVNFLLKNDEMRQGMIAAG
RKTVKEMSGALTRTLSALDPFIHPLTVKARLQGGDTGG

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=400, Percent_Identity=36.25, Blast_Score=228, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48317; Mature: 48186

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERWARAMLTTYRWAGAAAFPFVGGYVAWRVSKGKEDRARRHERYGRFDIPRPEGPLVW
CCHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEE
MHAASVGETMAVMGLIEHFLASGIKVVLTTGTVTSASVASERLGDRIIHQYVPLDLKPAV
EECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
SRFLNHWRPDLAVMAESEIWPLTILELGARRVPQVLVNGRMSDRSFARWQRRPSLAEVLF
HHHHHHCCCCEEEEECCCCCCHHHHHHCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHH
ENLAHVVAQSELDGARFRQLGARPVTVSGNLKVDTHVPPVDQSALDLLRRTINGRKTWAA
HHHHHHHHHHCCCHHHHHHCCCCEEEEECCEEECCCCCCCCHHHHHHHHHHHCCCCEEEE
VSTHEGEEMIAADVHMLVKKRHPDLLTIIVPRHPERCAALKAELAARGLKVALRNGGPLV
EECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCC
SPSTDILLGNSIGEMGLYLRLTEIAFVGRSMTGKGGQNPLEPAMLETAVLSGVNVENFRD
CCCCCEEECCCCCHHHHHEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
SYRRLIERGGAKLVRDSQMLAGAVNFLLKNDEMRQGMIAAGRKTVKEMSGALTRTLSALD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PFIHPLTVKARLQGGDTGG
HHHCCEEEEEEECCCCCCC
>Mature Secondary Structure 
SERWARAMLTTYRWAGAAAFPFVGGYVAWRVSKGKEDRARRHERYGRFDIPRPEGPLVW
CHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEE
MHAASVGETMAVMGLIEHFLASGIKVVLTTGTVTSASVASERLGDRIIHQYVPLDLKPAV
EECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
SRFLNHWRPDLAVMAESEIWPLTILELGARRVPQVLVNGRMSDRSFARWQRRPSLAEVLF
HHHHHHCCCCEEEEECCCCCCHHHHHHCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHH
ENLAHVVAQSELDGARFRQLGARPVTVSGNLKVDTHVPPVDQSALDLLRRTINGRKTWAA
HHHHHHHHHHCCCHHHHHHCCCCEEEEECCEEECCCCCCCCHHHHHHHHHHHCCCCEEEE
VSTHEGEEMIAADVHMLVKKRHPDLLTIIVPRHPERCAALKAELAARGLKVALRNGGPLV
EECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCC
SPSTDILLGNSIGEMGLYLRLTEIAFVGRSMTGKGGQNPLEPAMLETAVLSGVNVENFRD
CCCCCEEECCCCCHHHHHEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
SYRRLIERGGAKLVRDSQMLAGAVNFLLKNDEMRQGMIAAGRKTVKEMSGALTRTLSALD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PFIHPLTVKARLQGGDTGG
HHHCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]