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The map label for this gene is pmbA [H]

Identifier: 110632795

GI number: 110632795

Start: 481120

End: 482457

Strand: Direct

Name: pmbA [H]

Synonym: Meso_0434

Alternate gene names: 110632795

Gene position: 481120-482457 (Clockwise)

Preceding gene: 110632793

Following gene: 110632796

Centisome position: 10.9

GC content: 63.3

Gene sequence:

>1338_bases
ATGCCTCTCGATGAAAAAAGCCTCCTCGACCGCGTAACCAGCCTCGTGGAGGCAGCGAAACGCGCCGGCGCGGACGCCGC
CGATGCCGTGGTGATGCGTTCCCGCTCGAGCAGCGTCTCGGTTCGGCTTGGAAAGGTCGAAGGCACCGAATCGGCTGAAA
GCGACGATATGTCCTTGCGTGTCTTTGTCGGACGGCGTGTCGCGAGCGTTTCGGCGACGACGAGCTCCGACCCGGCGGTC
CTGGCCGAGCGGGCCGTGGCCATGGCGAAAGCCTCTCCCAAAGACCCCTATCAAGGGCTTGCCGACCGCCATCGGCTCGC
GCGCGAAGTCGCCGATCTCGATCTGGTCGATCCGACCGAGGTTTCCGCCGAACGCCTCAAGGAGGATGCGCTCGCCGCGG
AGGACGCCGCCCTTGCCGTGCCTGGCGTTACCAATTCTTCTGGAGCCGGCGCGAGCGCGGGAACAGGCGGTTTGGTGTTG
GCCACCTCTGACGGTTTCGTCGGTGAATACTCGGCCACGCGCTTCGGCCGCTCGCTGAGCGTCATTGCCGGCGAGGGCAC
GAAGATGGAGCGCGATTACGACTACTCGTCGCGGCTTCACTTCGCCGATCTCGACACGCCCGAGAAGCTGGGACGCACAG
CAGGAGAGCGTGCCGTCAAACGCCTCAACCCGCGCCGCGTGAAGACCGGTGTTTTTCCTGTGATCTACGATCCGCGGGTG
GCGCGCGGGATTGCCGGCCATATTGCTTCGGCGATCAACGGTGCGGCGGTCGCGCGCAAGACGACATTTTTACGGGAGAT
GATGGGCAAGAAGGTCGCTGCCGAAAACATCACGGTGACGGACGATCCCTTTCGGCGCCGGGGCTCGAATTCCCGGCCTT
TCGATGGCGAGGGCATTGCCGGTGTTCCGCTGGTGCCGGTGGAAAAAGGCGTTCTGACCACGTGGCTTCTCTCCAGCTCG
GTAGCGCGGGAACTGGGGCTTGAAACGAACGGACGCGGCGTGCGCGCCTCCTCGTCCGTCAATCCTTCCTGCACGAATCT
CGCCATCGAGCCGGGGGAAGGCTCTGCCGAGGACATGCTGGCTTCGGTCAAGAGCGGCTTTTATGTGACGGAGTTGTTCG
GCCATGGTGTGGATCTTGTCACCGGTGACTATAGCCGCGGCGCTTCCGGTTTTTGGATCGAGAACGGCGCGATTGCTTAT
CCGGTGTCGGAGATCACCATTGCATCGAATCTGAAGGACATGTTCCTGCGCATGACGCCCGCGAGCGATCTAGACCGGAA
CTACGGCATTGCCGCCCCGACGCTGCTCATCGAAGGGATGACGATTGCCGGTGAGTGA

Upstream 100 bases:

>100_bases
TGGAAGTCCATGTCCAAGTCTTGCCCACTCTCTCATCTCCTCCAAATTGTTGCCATAGCGCCGCAAGGCGGTCTTTTCGG
AATTCTGTCCCGGAGCCAAT

Downstream 100 bases:

>100_bases
GGCCTCCTTCACCCCTGCGCAAGAGCTTTCGCTGATCGTCGAGGCGGCGCGGGAAGCGGGTCGGATCGCGATGGGTTATT
TCCGGCGCGATCCGGAAGTG

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MPLDEKSLLDRVTSLVEAAKRAGADAADAVVMRSRSSSVSVRLGKVEGTESAESDDMSLRVFVGRRVASVSATTSSDPAV
LAERAVAMAKASPKDPYQGLADRHRLAREVADLDLVDPTEVSAERLKEDALAAEDAALAVPGVTNSSGAGASAGTGGLVL
ATSDGFVGEYSATRFGRSLSVIAGEGTKMERDYDYSSRLHFADLDTPEKLGRTAGERAVKRLNPRRVKTGVFPVIYDPRV
ARGIAGHIASAINGAAVARKTTFLREMMGKKVAAENITVTDDPFRRRGSNSRPFDGEGIAGVPLVPVEKGVLTTWLLSSS
VARELGLETNGRGVRASSSVNPSCTNLAIEPGEGSAEDMLASVKSGFYVTELFGHGVDLVTGDYSRGASGFWIENGAIAY
PVSEITIASNLKDMFLRMTPASDLDRNYGIAAPTLLIEGMTIAGE

Sequences:

>Translated_445_residues
MPLDEKSLLDRVTSLVEAAKRAGADAADAVVMRSRSSSVSVRLGKVEGTESAESDDMSLRVFVGRRVASVSATTSSDPAV
LAERAVAMAKASPKDPYQGLADRHRLAREVADLDLVDPTEVSAERLKEDALAAEDAALAVPGVTNSSGAGASAGTGGLVL
ATSDGFVGEYSATRFGRSLSVIAGEGTKMERDYDYSSRLHFADLDTPEKLGRTAGERAVKRLNPRRVKTGVFPVIYDPRV
ARGIAGHIASAINGAAVARKTTFLREMMGKKVAAENITVTDDPFRRRGSNSRPFDGEGIAGVPLVPVEKGVLTTWLLSSS
VARELGLETNGRGVRASSSVNPSCTNLAIEPGEGSAEDMLASVKSGFYVTELFGHGVDLVTGDYSRGASGFWIENGAIAY
PVSEITIASNLKDMFLRMTPASDLDRNYGIAAPTLLIEGMTIAGE
>Mature_444_residues
PLDEKSLLDRVTSLVEAAKRAGADAADAVVMRSRSSSVSVRLGKVEGTESAESDDMSLRVFVGRRVASVSATTSSDPAVL
AERAVAMAKASPKDPYQGLADRHRLAREVADLDLVDPTEVSAERLKEDALAAEDAALAVPGVTNSSGAGASAGTGGLVLA
TSDGFVGEYSATRFGRSLSVIAGEGTKMERDYDYSSRLHFADLDTPEKLGRTAGERAVKRLNPRRVKTGVFPVIYDPRVA
RGIAGHIASAINGAAVARKTTFLREMMGKKVAAENITVTDDPFRRRGSNSRPFDGEGIAGVPLVPVEKGVLTTWLLSSSV
ARELGLETNGRGVRASSSVNPSCTNLAIEPGEGSAEDMLASVKSGFYVTELFGHGVDLVTGDYSRGASGFWIENGAIAYP
VSEITIASNLKDMFLRMTPASDLDRNYGIAAPTLLIEGMTIAGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=448, Percent_Identity=38.6160714285714, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1789640, Length=217, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46832; Mature: 46701

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDEKSLLDRVTSLVEAAKRAGADAADAVVMRSRSSSVSVRLGKVEGTESAESDDMSLR
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEE
VFVGRRVASVSATTSSDPAVLAERAVAMAKASPKDPYQGLADRHRLAREVADLDLVDPTE
EEECCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
VSAERLKEDALAAEDAALAVPGVTNSSGAGASAGTGGLVLATSDGFVGEYSATRFGRSLS
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCEEE
VIAGEGTKMERDYDYSSRLHFADLDTPEKLGRTAGERAVKRLNPRRVKTGVFPVIYDPRV
EEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEEEECCHH
ARGIAGHIASAINGAAVARKTTFLREMMGKKVAAENITVTDDPFRRRGSNSRPFDGEGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEECCCHHHHCCCCCCCCCCCCCC
GVPLVPVEKGVLTTWLLSSSVARELGLETNGRGVRASSSVNPSCTNLAIEPGEGSAEDML
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCEEEEECCCCCHHHHH
ASVKSGFYVTELFGHGVDLVTGDYSRGASGFWIENGAIAYPVSEITIASNLKDMFLRMTP
HHHHCCCEEEHHHHCCCEEEECCCCCCCCCEEEECCEEEECHHHEEHHHHHHHHHEECCC
ASDLDRNYGIAAPTLLIEGMTIAGE
CHHCCCCCCCCCHHHHEECEEECCC
>Mature Secondary Structure 
PLDEKSLLDRVTSLVEAAKRAGADAADAVVMRSRSSSVSVRLGKVEGTESAESDDMSLR
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEE
VFVGRRVASVSATTSSDPAVLAERAVAMAKASPKDPYQGLADRHRLAREVADLDLVDPTE
EEECCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
VSAERLKEDALAAEDAALAVPGVTNSSGAGASAGTGGLVLATSDGFVGEYSATRFGRSLS
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCEEE
VIAGEGTKMERDYDYSSRLHFADLDTPEKLGRTAGERAVKRLNPRRVKTGVFPVIYDPRV
EEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEEEECCHH
ARGIAGHIASAINGAAVARKTTFLREMMGKKVAAENITVTDDPFRRRGSNSRPFDGEGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEECCCHHHHCCCCCCCCCCCCCC
GVPLVPVEKGVLTTWLLSSSVARELGLETNGRGVRASSSVNPSCTNLAIEPGEGSAEDML
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCEEEEECCCCCHHHHH
ASVKSGFYVTELFGHGVDLVTGDYSRGASGFWIENGAIAYPVSEITIASNLKDMFLRMTP
HHHHCCCEEEHHHHCCCEEEECCCCCCCCCEEEECCEEEECHHHEEHHHHHHHHHEECCC
ASDLDRNYGIAAPTLLIEGMTIAGE
CHHCCCCCCCCCHHHHEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]