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The map label for this gene is pucI [H]

Identifier: 110632696

GI number: 110632696

Start: 371352

End: 372803

Strand: Direct

Name: pucI [H]

Synonym: Meso_0335

Alternate gene names: 110632696

Gene position: 371352-372803 (Clockwise)

Preceding gene: 110632695

Following gene: 110632701

Centisome position: 8.42

GC content: 57.64

Gene sequence:

>1452_bases
ATGGCAATTTCATCCGGCAAGCACGCCCAGGATGGCGCGCTTTATAATACGGACTTGGCCCCGCTGCCGCCGGAGAGGCG
CAAATGGGGCGCTTTTGAAATCTTCAACGTATGGAATAACGATATACAGAGCCTATTCGGCTATTCGTTAGCCGCCTCGC
TCTTCATTACATACGGGCTCAATGGCTGGGCGGTCTTCGCCGCGATCATCGTCGCCGGCTTTTTTGTGATGTTCCTGGTC
AACCTCACCGGCAAACCATCGGTCAAGTATGGGGTCCCTTACCCCGTCATGGCCAGGGCCAGCATGGGGGTCTATGGTGC
GCGCTTTCCGGCCCTGATCCGCGGCATTGTCGCGATTTTCTGGTACGGCGTGCAGACCTATTTCGCCTCGACCGCAGTAG
CACTGCTGTTGAATACCATGCTCGGCATCCGAGGCGGGGCCGAATTCCTGGGCCTGACGGCCGTAGGCTGGCTCTCCTAT
TTCATCGTCTGCGCGTTCCAGGTCATGCTTTTCGCGCGCGGCATCGACTGGGTCGGAAAGTTTCTGAACTGGGCCGGTCC
CACCGTCTATGCGGTGATGATCGCTCTTTGTCTCGTCATCTGGTGGAAAGCGGGCAACGGCATCTTCAGCGAGCTCGGCG
CGATCTTCGAGGGCGGTGAGGCATCCGATCAGCGGGGCTCGGTGGCCGCGTTTTTCGCTATCGTGGGAACGATGATCGCC
TATTTCGCGGCGGTCGTGATAAATTTCGGAGACTTCTCGCGTTTCGTCAAAACCGAGTCGGCGATGAAGATCGGCAATTT
CTGGGGCCTGCCGATCTCCCTGGCGTTCTTTTCCTTCATCGCGCTCTTCGTTACGGCCGGCACCGTGGTGTTGTTCGGCG
AGAGGCTCATCAATCCCGCCGATATCGTCGACCGTGTCGATAATGTGCTGCTTACGCTCATTGCCGCCCTTACATTTTTC
GCGGCGACCGTCGGTATCAACCTCGTTGCCAACTTCATTCCTCCGGCCTACGACATCGCCAATCTCAATCCGGAGAAAAT
TTCCGCGAAGATGGGCGGCGTTATCACGGCAATCGTCGCATTCTTCATCGGCGCGCTCTGGGTGCCGGTGATCTCCACTA
TCGGCATCACCGCCTTCGTCGACACGCTTGGAGCATTCCTTGCGCCGCTTTACGGCATTCTCGTCGCCGACTACTACATC
GTTCGTCGCAAGAATATTTCCGTAGACGATTTGTTCTCGTCCGATCCTTCAGGGCCTTATTTCTACACCGGTGGCTGGAA
CCAGAAGGCTATCATCGCCTTCGCTATTCCGGCCGTCTTTTCGGTGGCCACGGTATGGATCCCCGCACTTTCGCAGCTGT
CGGGTTACGGCTGGGTGATCGGCGCGATACTGGGTGGTGCTGTGCATGTCGGCCTGATGCACAGCAAAATGCCGGCTCCG
AGAACGATCTGA

Upstream 100 bases:

>100_bases
CGGCGTAACCTCTCGGGAAAAATAATAAAAACGCCAGCAATGGTTTTTGTGTCTGTCGTACCATTGCGGTGTCCTGAGGG
CTCAACAATGGGAGAGGGTG

Downstream 100 bases:

>100_bases
TCGGCTCTGGTTAAGCCGAGCTATTCTCCCGGCGGTGGCGATCGTGCGCCACCGCTTCGCGATGGCGGCGGGCGGATAAC
TGGGCTCGCCGCTTTTGCTC

Product: permease for cytosine/purines, uracil, thiamine, allantoin

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAISSGKHAQDGALYNTDLAPLPPERRKWGAFEIFNVWNNDIQSLFGYSLAASLFITYGLNGWAVFAAIIVAGFFVMFLV
NLTGKPSVKYGVPYPVMARASMGVYGARFPALIRGIVAIFWYGVQTYFASTAVALLLNTMLGIRGGAEFLGLTAVGWLSY
FIVCAFQVMLFARGIDWVGKFLNWAGPTVYAVMIALCLVIWWKAGNGIFSELGAIFEGGEASDQRGSVAAFFAIVGTMIA
YFAAVVINFGDFSRFVKTESAMKIGNFWGLPISLAFFSFIALFVTAGTVVLFGERLINPADIVDRVDNVLLTLIAALTFF
AATVGINLVANFIPPAYDIANLNPEKISAKMGGVITAIVAFFIGALWVPVISTIGITAFVDTLGAFLAPLYGILVADYYI
VRRKNISVDDLFSSDPSGPYFYTGGWNQKAIIAFAIPAVFSVATVWIPALSQLSGYGWVIGAILGGAVHVGLMHSKMPAP
RTI

Sequences:

>Translated_483_residues
MAISSGKHAQDGALYNTDLAPLPPERRKWGAFEIFNVWNNDIQSLFGYSLAASLFITYGLNGWAVFAAIIVAGFFVMFLV
NLTGKPSVKYGVPYPVMARASMGVYGARFPALIRGIVAIFWYGVQTYFASTAVALLLNTMLGIRGGAEFLGLTAVGWLSY
FIVCAFQVMLFARGIDWVGKFLNWAGPTVYAVMIALCLVIWWKAGNGIFSELGAIFEGGEASDQRGSVAAFFAIVGTMIA
YFAAVVINFGDFSRFVKTESAMKIGNFWGLPISLAFFSFIALFVTAGTVVLFGERLINPADIVDRVDNVLLTLIAALTFF
AATVGINLVANFIPPAYDIANLNPEKISAKMGGVITAIVAFFIGALWVPVISTIGITAFVDTLGAFLAPLYGILVADYYI
VRRKNISVDDLFSSDPSGPYFYTGGWNQKAIIAFAIPAVFSVATVWIPALSQLSGYGWVIGAILGGAVHVGLMHSKMPAP
RTI
>Mature_482_residues
AISSGKHAQDGALYNTDLAPLPPERRKWGAFEIFNVWNNDIQSLFGYSLAASLFITYGLNGWAVFAAIIVAGFFVMFLVN
LTGKPSVKYGVPYPVMARASMGVYGARFPALIRGIVAIFWYGVQTYFASTAVALLLNTMLGIRGGAEFLGLTAVGWLSYF
IVCAFQVMLFARGIDWVGKFLNWAGPTVYAVMIALCLVIWWKAGNGIFSELGAIFEGGEASDQRGSVAAFFAIVGTMIAY
FAAVVINFGDFSRFVKTESAMKIGNFWGLPISLAFFSFIALFVTAGTVVLFGERLINPADIVDRVDNVLLTLIAALTFFA
ATVGINLVANFIPPAYDIANLNPEKISAKMGGVITAIVAFFIGALWVPVISTIGITAFVDTLGAFLAPLYGILVADYYIV
RRKNISVDDLFSSDPSGPYFYTGGWNQKAIIAFAIPAVFSVATVWIPALSQLSGYGWVIGAILGGAVHVGLMHSKMPAPR
TI

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=473, Percent_Identity=28.7526427061311, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319495, Length=445, Percent_Identity=25.1685393258427, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322219, Length=444, Percent_Identity=25.6756756756757, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319429, Length=442, Percent_Identity=26.2443438914027, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324766, Length=442, Percent_Identity=21.9457013574661, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323266, Length=440, Percent_Identity=20.2272727272727, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 51986; Mature: 51854

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCC
>Mature Secondary Structure 
AISSGKHAQDGALYNTDLAPLPPERRKWGAFEIFNVWNNDIQSLFGYSLAASLFITYGL
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC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CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]