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The map label for this gene is pucI [H]

Identifier: 110632694

GI number: 110632694

Start: 368883

End: 370238

Strand: Reverse

Name: pucI [H]

Synonym: Meso_0333

Alternate gene names: 110632694

Gene position: 370238-368883 (Counterclockwise)

Preceding gene: 110632697

Following gene: 110632693

Centisome position: 8.39

GC content: 60.18

Gene sequence:

>1356_bases
ATGGCAAATACCCGGGAATTTGTGGCGCCGCCTCAAATCGGCGCCCACAGCAAGAATATCGGCGAGGAATCGCTCGCTCC
GCAGCAGACCCGAATCATGGGGCCCACATCCTACCTGCTCGCATGGATGGGTGGTTGCGTCTCGATCGGCACCTTCACGA
TGGGCTCCTCGATCGTCGGCACGCTCAACCTGCTGCAGGCCACCATCGCCATCGCTATCGGCTGCCTCGTTATCGGCGTC
GCGCTGACCATCAACGGTGCCGCCGGCTATAAATACGGCATCCCCTTCATGGTGCAGGCGCGCAGCGCCTTCGGTTTCAC
CGGCACGGGATTTCCCGGCATCGTTCGCGCCGTGCCGGCCATCGTATGGTACGGCTTCCAGAGCTGGATCGGTGCGGGCG
CGCTGAATGCAGTTACCGCGACCATGTTCGGCTTCGACAATCTCGTCGCCTGGTTCATCATCTTCCAGCTATTGCAAATC
GGCCTTTCGGTGCTCGGTTTCCAAGGCATCAAATGGCTGGAGAATATCGGCGCGGTCTTCATCCTCTTTTCGCTGTTTTA
CATGTTCTTCAGCGTGATCAACCGCTACGGCGCCGAAATCACCGCCAACCTCGTCAATATCGAGGGAACCTGGGGCCTGC
CCTTCTGGGGCGCAACGATGCTCTTTCTGGGCATCTACTCGACGATGATCCTGAACGTCAGCGATTACGCGCGCGAGCAC
ACGCACGGCTCCTCGCCGAGCCTGCTCGTCACCATCTATTCGATGTCGATCCTGCCGGTGACACTGTTCATGGGGCTGAT
CGGGCTGATGGTCTCCGGCGCCACCGGCGTCACGGACCCGATCCAGGTCTTCGCCAACGCGGTCGACAACAAACCACTTT
TGATCGTGACGCTGCTCTTCATCGCCTTCGCGCAGGTCACCACCAATGTTCTCAACAATGTCATCCCGCCAACCTATGTG
CTGATGGACATCTTCAAACTGCCCTTCAAGCTCTCGACCGTTATCGTCGGCTTTCTGGCCTTCGCCACCTTTCCGTGGGA
GCTGGTCAAGGACGAAAGCGCGGCCGGCCTGCAGATCTTCGTGCAGACTTACTCCGCCTTCCTCGGCCCGATCTTCGCGG
TCATGGTGGTCGATTATTACCTGCTGCGCAAACGCACGCTGGATGTCGGGAAATTGTACGATGAGACCGGTCCCTATAGC
GGCGTCAACTGGGCGGCGATCATCGCCTCGCTGATCGGCGCAGTCGTCGCGCTGACCTTCTCCGCTGTCTCGTGGTACGC
GAGCCTCATCCCCGCCGGCGTGATCTACTGGCTGCTGATGCAGTACTGGGGGCCTGCGCAGCGCTTCCGCGCCTGA

Upstream 100 bases:

>100_bases
TCGCTGACATGCGCGATCTCTCTGCTGGAGTGCCGATGGCTGGATGGAGGACAGTGCCAGCCCCGGACGAATGTCTCCGG
TGGGAATGGGAGGTAATCAA

Downstream 100 bases:

>100_bases
CCCGAACCGGCGCTCCGGCGGGGCATGCCTGCCGGGCGCCATTATGTGAACGACTATCGGAGACCCCACCCATGCGCATC
AGGGTGATCAATCCCAACAC

Product: permease for cytosine/purines, uracil, thiamine, allantoin

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MANTREFVAPPQIGAHSKNIGEESLAPQQTRIMGPTSYLLAWMGGCVSIGTFTMGSSIVGTLNLLQATIAIAIGCLVIGV
ALTINGAAGYKYGIPFMVQARSAFGFTGTGFPGIVRAVPAIVWYGFQSWIGAGALNAVTATMFGFDNLVAWFIIFQLLQI
GLSVLGFQGIKWLENIGAVFILFSLFYMFFSVINRYGAEITANLVNIEGTWGLPFWGATMLFLGIYSTMILNVSDYAREH
THGSSPSLLVTIYSMSILPVTLFMGLIGLMVSGATGVTDPIQVFANAVDNKPLLIVTLLFIAFAQVTTNVLNNVIPPTYV
LMDIFKLPFKLSTVIVGFLAFATFPWELVKDESAAGLQIFVQTYSAFLGPIFAVMVVDYYLLRKRTLDVGKLYDETGPYS
GVNWAAIIASLIGAVVALTFSAVSWYASLIPAGVIYWLLMQYWGPAQRFRA

Sequences:

>Translated_451_residues
MANTREFVAPPQIGAHSKNIGEESLAPQQTRIMGPTSYLLAWMGGCVSIGTFTMGSSIVGTLNLLQATIAIAIGCLVIGV
ALTINGAAGYKYGIPFMVQARSAFGFTGTGFPGIVRAVPAIVWYGFQSWIGAGALNAVTATMFGFDNLVAWFIIFQLLQI
GLSVLGFQGIKWLENIGAVFILFSLFYMFFSVINRYGAEITANLVNIEGTWGLPFWGATMLFLGIYSTMILNVSDYAREH
THGSSPSLLVTIYSMSILPVTLFMGLIGLMVSGATGVTDPIQVFANAVDNKPLLIVTLLFIAFAQVTTNVLNNVIPPTYV
LMDIFKLPFKLSTVIVGFLAFATFPWELVKDESAAGLQIFVQTYSAFLGPIFAVMVVDYYLLRKRTLDVGKLYDETGPYS
GVNWAAIIASLIGAVVALTFSAVSWYASLIPAGVIYWLLMQYWGPAQRFRA
>Mature_450_residues
ANTREFVAPPQIGAHSKNIGEESLAPQQTRIMGPTSYLLAWMGGCVSIGTFTMGSSIVGTLNLLQATIAIAIGCLVIGVA
LTINGAAGYKYGIPFMVQARSAFGFTGTGFPGIVRAVPAIVWYGFQSWIGAGALNAVTATMFGFDNLVAWFIIFQLLQIG
LSVLGFQGIKWLENIGAVFILFSLFYMFFSVINRYGAEITANLVNIEGTWGLPFWGATMLFLGIYSTMILNVSDYAREHT
HGSSPSLLVTIYSMSILPVTLFMGLIGLMVSGATGVTDPIQVFANAVDNKPLLIVTLLFIAFAQVTTNVLNNVIPPTYVL
MDIFKLPFKLSTVIVGFLAFATFPWELVKDESAAGLQIFVQTYSAFLGPIFAVMVVDYYLLRKRTLDVGKLYDETGPYSG
VNWAAIIASLIGAVVALTFSAVSWYASLIPAGVIYWLLMQYWGPAQRFRA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=468, Percent_Identity=24.1452991452991, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319495, Length=444, Percent_Identity=23.1981981981982, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322219, Length=438, Percent_Identity=22.8310502283105, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319429, Length=438, Percent_Identity=24.6575342465753, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 48894; Mature: 48763

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTREFVAPPQIGAHSKNIGEESLAPQQTRIMGPTSYLLAWMGGCVSIGTFTMGSSIVG
CCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCHHHHHHHHHCCHHHHHHHHHCHHHHH
TLNLLQATIAIAIGCLVIGVALTINGAAGYKYGIPFMVQARSAFGFTGTGFPGIVRAVPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEECCCCCCCCCCCHHHHHHHHH
IVWYGFQSWIGAGALNAVTATMFGFDNLVAWFIIFQLLQIGLSVLGFQGIKWLENIGAVF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILFSLFYMFFSVINRYGAEITANLVNIEGTWGLPFWGATMLFLGIYSTMILNVSDYAREH
HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
THGSSPSLLVTIYSMSILPVTLFMGLIGLMVSGATGVTDPIQVFANAVDNKPLLIVTLLF
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
IAFAQVTTNVLNNVIPPTYVLMDIFKLPFKLSTVIVGFLAFATFPWELVKDESAAGLQIF
HHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHH
VQTYSAFLGPIFAVMVVDYYLLRKRTLDVGKLYDETGPYSGVNWAAIIASLIGAVVALTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
SAVSWYASLIPAGVIYWLLMQYWGPAQRFRA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
ANTREFVAPPQIGAHSKNIGEESLAPQQTRIMGPTSYLLAWMGGCVSIGTFTMGSSIVG
CCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCHHHHHHHHHCCHHHHHHHHHCHHHHH
TLNLLQATIAIAIGCLVIGVALTINGAAGYKYGIPFMVQARSAFGFTGTGFPGIVRAVPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEECCCCCCCCCCCHHHHHHHHH
IVWYGFQSWIGAGALNAVTATMFGFDNLVAWFIIFQLLQIGLSVLGFQGIKWLENIGAVF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILFSLFYMFFSVINRYGAEITANLVNIEGTWGLPFWGATMLFLGIYSTMILNVSDYAREH
HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
THGSSPSLLVTIYSMSILPVTLFMGLIGLMVSGATGVTDPIQVFANAVDNKPLLIVTLLF
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
IAFAQVTTNVLNNVIPPTYVLMDIFKLPFKLSTVIVGFLAFATFPWELVKDESAAGLQIF
HHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHH
VQTYSAFLGPIFAVMVVDYYLLRKRTLDVGKLYDETGPYSGVNWAAIIASLIGAVVALTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
SAVSWYASLIPAGVIYWLLMQYWGPAQRFRA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]