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The map label for this gene is potI [H]

Identifier: 110632528

GI number: 110632528

Start: 202715

End: 203533

Strand: Direct

Name: potI [H]

Synonym: Meso_0166

Alternate gene names: 110632528

Gene position: 202715-203533 (Clockwise)

Preceding gene: 110632527

Following gene: 110632530

Centisome position: 4.59

GC content: 59.83

Gene sequence:

>819_bases
ATGGCTGGCACATGGGGACGTTTCAATGTCGCTGCGCTGGTTCTGGGATTTGCATTCCTTTACCTTCCGATCGTCCTGCT
GGTGATCTATTCATTCAACGAATCCCGCCTCGTGACGGTCTGGGCGGGGTTCTCGACGCGATGGTACGTCTCGCTTCTGT
CAAATCGCGGATTGATGGAGGCCGCCTGGGTGACGGCGCGGATCGCTTTCATCTCGGCCACGGTGGCAACCGTACTGGGA
ACAATGGCGGCCGTGGCCTTGACGCGCTACACGCGGTTTCGTGGGCGGCTGCTGTTTTCGGGAATGGTATTCGCGCCTTT
GGTTATGCCGGAGGTGATCACCGGGCTGTCGCTGCTGCTCCTCTTTGTAGCGATCGGGCTCGACCGCGGGTTCATGACGG
TCACTCTGGCCCACATTACCTTCTCGATGTGCTTCGTTGCCGTGGTGGTGCAATCGCGGCTGGTGTCATTCGACCGCTCG
CTGGAGGAGGCGGCGATGGATTTGGGCGCCACGCCAATGCGGGCGTTCTTCCAGGTCACGCTGCCGATCATTCTCCCGGC
GGTGATTTCGGGCTGGATGCTTGCCTTCACCCTTTCGCTCGATGATCTGGTGATTGCGAGCTTTACGTCGGGGCCGGGTG
CCACGACCCTGCCCATGAAAATTTATAGCCAGGTGCGCCTGGGTGTTACGCCGGAAATTAACGCCGTCTGCACGATTTTG
ATCGGCGTGGTGACCATTGGGGTCATCGCGGCCTCGATTACAAACAAGCGGCGTGAGTTGCGGCGCCGGCGAGAGGAACT
GCTAGCGTTCGGCTCTTAA

Upstream 100 bases:

>100_bases
TTTCTTCAGCAGTGGCGATCGTGCTCCTGCTCCTTCTGGTATTGCCTATCGTCTACTTCCAGCGTGCCCAGGCGCGTGCG
CAGGAAAAGGCGGGTTGACC

Downstream 100 bases:

>100_bases
GGCCTTCAGGGAGTAGATTGGCTTCTCAGTCTTCCGCCGGGAGGAAGGAGGCGATGAATGGGGCCGCCCAGTTGCCTCCT
ACAAAGAAGGAGAGGGGCGA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MAGTWGRFNVAALVLGFAFLYLPIVLLVIYSFNESRLVTVWAGFSTRWYVSLLSNRGLMEAAWVTARIAFISATVATVLG
TMAAVALTRYTRFRGRLLFSGMVFAPLVMPEVITGLSLLLLFVAIGLDRGFMTVTLAHITFSMCFVAVVVQSRLVSFDRS
LEEAAMDLGATPMRAFFQVTLPIILPAVISGWMLAFTLSLDDLVIASFTSGPGATTLPMKIYSQVRLGVTPEINAVCTIL
IGVVTIGVIAASITNKRRELRRRREELLAFGS

Sequences:

>Translated_272_residues
MAGTWGRFNVAALVLGFAFLYLPIVLLVIYSFNESRLVTVWAGFSTRWYVSLLSNRGLMEAAWVTARIAFISATVATVLG
TMAAVALTRYTRFRGRLLFSGMVFAPLVMPEVITGLSLLLLFVAIGLDRGFMTVTLAHITFSMCFVAVVVQSRLVSFDRS
LEEAAMDLGATPMRAFFQVTLPIILPAVISGWMLAFTLSLDDLVIASFTSGPGATTLPMKIYSQVRLGVTPEINAVCTIL
IGVVTIGVIAASITNKRRELRRRREELLAFGS
>Mature_271_residues
AGTWGRFNVAALVLGFAFLYLPIVLLVIYSFNESRLVTVWAGFSTRWYVSLLSNRGLMEAAWVTARIAFISATVATVLGT
MAAVALTRYTRFRGRLLFSGMVFAPLVMPEVITGLSLLLLFVAIGLDRGFMTVTLAHITFSMCFVAVVVQSRLVSFDRSL
EEAAMDLGATPMRAFFQVTLPIILPAVISGWMLAFTLSLDDLVIASFTSGPGATTLPMKIYSQVRLGVTPEINAVCTILI
GVVTIGVIAASITNKRRELRRRREELLAFGS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=264, Percent_Identity=59.8484848484849, Blast_Score=270, Evalue=6e-74,
Organism=Escherichia coli, GI1787368, Length=230, Percent_Identity=45.2173913043478, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1787714, Length=239, Percent_Identity=33.8912133891213, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788764, Length=175, Percent_Identity=32, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29689; Mature: 29558

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGTWGRFNVAALVLGFAFLYLPIVLLVIYSFNESRLVTVWAGFSTRWYVSLLSNRGLME
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCCHH
AAWVTARIAFISATVATVLGTMAAVALTRYTRFRGRLLFSGMVFAPLVMPEVITGLSLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFVAIGLDRGFMTVTLAHITFSMCFVAVVVQSRLVSFDRSLEEAAMDLGATPMRAFFQVT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LPIILPAVISGWMLAFTLSLDDLVIASFTSGPGATTLPMKIYSQVRLGVTPEINAVCTIL
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
IGVVTIGVIAASITNKRRELRRRREELLAFGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]