Click here to switch to the map view.

The map label for this gene is acsA1 [H]

Identifier: 110632530

GI number: 110632530

Start: 205572

End: 207530

Strand: Direct

Name: acsA1 [H]

Synonym: Meso_0168

Alternate gene names: 110632530

Gene position: 205572-207530 (Clockwise)

Preceding gene: 110632528

Following gene: 110632534

Centisome position: 4.66

GC content: 59.62

Gene sequence:

>1959_bases
ATGAAGCAAGCAATGCCGCTTTGGATCCCATCGGCCGAGCAGATCGAAAAGGCGCCGATGACCGCCTTCATTCGAGAGGC
AGTTCACCGGACGGGCCGGTCTATCGATGGTCATGCCGCGCTTCATCGCTGGTCCGTCGATCACCGGGAAGAGTTCTGGC
AACTCGTGTGGGATTTCTGCGGCATCATCGGCGAGCGCGGAGAGCGCGTGCTTATCGATGGCGACAAGATGCCCGGGGCC
GCCTATTTCCCCGACGCTAAGCTGAATTTCGCTGAGAACCTGCTTTGGAAGTCCGGCAATGGCGATGCGATAGTCTTCCG
CGGAGAAGACAAGGTGGAGCGGCGGATCTCGTGGCATGAGTTGAAGGCGCTCGTTTCTCGCTTGCAGCAGCTCTTCAAGG
CGGAGGGTGTAAAGGCAGGTGACCGCATCGCCGGAATGATGCCGAATATGCCCGAAACTGTTGCAGCCATGCTTGCGGCC
GCATCGCTCGGCGCGGTTTGGTCCTCCTGCTCGCCGGATTTCGGCGTGCGCGGCGTGCTCGACCGCTTCGGTCAGATCGA
GCCTGTGCTCTTCATCGCGCCGGACGGCTATTGGTATGGCGGAAAAGCTTTCGACGTGGGCCAAAAGACGGCCGAGATTC
TTTCGGCGCTGCCGAGCGTGAGGAAAGCTTTGATCGTCGACTATCTGGGCAGCGCGCGGAATGCTGCATCCGGCATCGAG
CGTGCGGTTGCGCTGGATGATGCAATTGCTCCATTCTCGGCGGGGCCGCTCACCTATGAGCGGCTGCCCTTTGCGCACCC
GCTCTATATCCTCTTTTCCTCGGGCACTACGGGCATCCCCAAATGCATCGTCCACTCGGCCGGCGGAACGCTGCTCCAAC
ATGTGAAGGAGCATCGTTTGCATGCCGGGCTCGCAGATGGAGACCGCTTCTTCTATTTCACGACCTGCGGGTGGATGATG
TGGAACTGGCTGGTCACCGGGCTTGCTTCGGGTGCAACGCTTTTGCTCTACGACGGCTCTCCCTTTTATCCGGACGGAAA
CATACTCTTCGATTTCGCGGCCGGCGAGCGGATGACCTATTTCGGTACGTCGGCAAAGTTCATCGACGCGGTACGCAAGG
CCGGACTTGAGCCGGCGAGAACGCATGATCTTTCAAGCATACGCGTGATTTCCTCGACAGGTTCGCCGCTTTCGCCGGAA
TGCTTCGAGTTCGTTTATCATTCGATCAAGAAGGACGTGCATCTGGCTTCGATCTCCGGGGGGACCGATATCGTGTCCTG
TTTCGTACTGGGGATCCCCCTGCTTCCCGTCTGGAGCGGTGAGATACAGGGGCCCGGTCTTGGCATGGCCATTGAAGTCT
GGGACGACCAGGGCCATCCGCTGGAGAGCGGGAAAGGGGAGTTGGTCTGCACGAAGGCGTTCCCCTCCATGCCGATCGCC
TTCTGGAACGATCCGTCGGGCGAGAAATACCGATCCGCCTATTTCGACCGATTCGAGAATATCTGGTGCCATGGCGATTT
CGCCGAATGGACGCCGCATGGAGGTATCGTCATTCACGGCCGCTCCGACGCCACGCTTAATCCCGGCGGCGTGCGCATCG
GCACGGCGGAGATTTACAATCAGGTCGAGCAGCTTCCGGAAATATTGGAGGCAATCTGTATCGGCCAGGATTGGGACGGC
GACGTGCGCGTGGTCCTGTTCGTGCGGCTTGCCGAGGGTTTGATGCTCGATGAGGCCCTGCAAAAGAAAATCCGGGATCG
CATCAGAACAGGCGCAAGCCCGCGCCATGTGCCGGCCAAGATCGTGGCGGTGAAGGATATTCCGCGCACGAAATCCGGCA
AGATCACCGAGCTTGCCGTGCGCGACGTGGTGCATGGACGCGAGGTGAAGAACAAGGAGGCGCTCGCAAATCCGGAGGCT
TTGGAACTCTACCGCGAGCTTCCTGAGCTTAAGGATTGA

Upstream 100 bases:

>100_bases
TTAGGGACCTGCGATCTGGGGTGGCAAGCCTGAAGGCTATCCCTGCTTGCTCGTTTGGGTGAGTCGTTGCATAAGCGGCG
AGTTTGATGGAGCACGAAAC

Downstream 100 bases:

>100_bases
GGCGTCTTACGTGCGAGCTTACCGCTCTACCAAGGTTGATGGAGCGTTGCGCGGGACTTTACCGTAATCCGACGGCGAAC
CACGCAGTTGCAATTTAACT

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MKQAMPLWIPSAEQIEKAPMTAFIREAVHRTGRSIDGHAALHRWSVDHREEFWQLVWDFCGIIGERGERVLIDGDKMPGA
AYFPDAKLNFAENLLWKSGNGDAIVFRGEDKVERRISWHELKALVSRLQQLFKAEGVKAGDRIAGMMPNMPETVAAMLAA
ASLGAVWSSCSPDFGVRGVLDRFGQIEPVLFIAPDGYWYGGKAFDVGQKTAEILSALPSVRKALIVDYLGSARNAASGIE
RAVALDDAIAPFSAGPLTYERLPFAHPLYILFSSGTTGIPKCIVHSAGGTLLQHVKEHRLHAGLADGDRFFYFTTCGWMM
WNWLVTGLASGATLLLYDGSPFYPDGNILFDFAAGERMTYFGTSAKFIDAVRKAGLEPARTHDLSSIRVISSTGSPLSPE
CFEFVYHSIKKDVHLASISGGTDIVSCFVLGIPLLPVWSGEIQGPGLGMAIEVWDDQGHPLESGKGELVCTKAFPSMPIA
FWNDPSGEKYRSAYFDRFENIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQLPEILEAICIGQDWDG
DVRVVLFVRLAEGLMLDEALQKKIRDRIRTGASPRHVPAKIVAVKDIPRTKSGKITELAVRDVVHGREVKNKEALANPEA
LELYRELPELKD

Sequences:

>Translated_652_residues
MKQAMPLWIPSAEQIEKAPMTAFIREAVHRTGRSIDGHAALHRWSVDHREEFWQLVWDFCGIIGERGERVLIDGDKMPGA
AYFPDAKLNFAENLLWKSGNGDAIVFRGEDKVERRISWHELKALVSRLQQLFKAEGVKAGDRIAGMMPNMPETVAAMLAA
ASLGAVWSSCSPDFGVRGVLDRFGQIEPVLFIAPDGYWYGGKAFDVGQKTAEILSALPSVRKALIVDYLGSARNAASGIE
RAVALDDAIAPFSAGPLTYERLPFAHPLYILFSSGTTGIPKCIVHSAGGTLLQHVKEHRLHAGLADGDRFFYFTTCGWMM
WNWLVTGLASGATLLLYDGSPFYPDGNILFDFAAGERMTYFGTSAKFIDAVRKAGLEPARTHDLSSIRVISSTGSPLSPE
CFEFVYHSIKKDVHLASISGGTDIVSCFVLGIPLLPVWSGEIQGPGLGMAIEVWDDQGHPLESGKGELVCTKAFPSMPIA
FWNDPSGEKYRSAYFDRFENIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQLPEILEAICIGQDWDG
DVRVVLFVRLAEGLMLDEALQKKIRDRIRTGASPRHVPAKIVAVKDIPRTKSGKITELAVRDVVHGREVKNKEALANPEA
LELYRELPELKD
>Mature_652_residues
MKQAMPLWIPSAEQIEKAPMTAFIREAVHRTGRSIDGHAALHRWSVDHREEFWQLVWDFCGIIGERGERVLIDGDKMPGA
AYFPDAKLNFAENLLWKSGNGDAIVFRGEDKVERRISWHELKALVSRLQQLFKAEGVKAGDRIAGMMPNMPETVAAMLAA
ASLGAVWSSCSPDFGVRGVLDRFGQIEPVLFIAPDGYWYGGKAFDVGQKTAEILSALPSVRKALIVDYLGSARNAASGIE
RAVALDDAIAPFSAGPLTYERLPFAHPLYILFSSGTTGIPKCIVHSAGGTLLQHVKEHRLHAGLADGDRFFYFTTCGWMM
WNWLVTGLASGATLLLYDGSPFYPDGNILFDFAAGERMTYFGTSAKFIDAVRKAGLEPARTHDLSSIRVISSTGSPLSPE
CFEFVYHSIKKDVHLASISGGTDIVSCFVLGIPLLPVWSGEIQGPGLGMAIEVWDDQGHPLESGKGELVCTKAFPSMPIA
FWNDPSGEKYRSAYFDRFENIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQLPEILEAICIGQDWDG
DVRVVLFVRLAEGLMLDEALQKKIRDRIRTGASPRHVPAKIVAVKDIPRTKSGKITELAVRDVVHGREVKNKEALANPEA
LELYRELPELKD

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=657, Percent_Identity=43.8356164383562, Blast_Score=563, Evalue=1e-160,
Organism=Homo sapiens, GI28416953, Length=596, Percent_Identity=26.6778523489933, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI8923896, Length=656, Percent_Identity=25.9146341463415, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI253970489, Length=668, Percent_Identity=24.8502994011976, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI13375727, Length=605, Percent_Identity=23.4710743801653, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI38505220, Length=502, Percent_Identity=21.3147410358566, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI122937307, Length=549, Percent_Identity=21.4936247723133, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1790505, Length=577, Percent_Identity=27.5563258232236, Blast_Score=188, Evalue=9e-49,
Organism=Escherichia coli, GI1786529, Length=645, Percent_Identity=23.5658914728682, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17569507, Length=628, Percent_Identity=38.2165605095541, Blast_Score=446, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71982997, Length=624, Percent_Identity=24.8397435897436, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71983001, Length=624, Percent_Identity=24.8397435897436, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6323182, Length=645, Percent_Identity=26.2015503875969, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319264, Length=588, Percent_Identity=27.0408163265306, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24667955, Length=646, Percent_Identity=26.3157894736842, Blast_Score=198, Evalue=1e-50,
Organism=Drosophila melanogaster, GI62472339, Length=557, Percent_Identity=26.9299820466786, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24667959, Length=520, Percent_Identity=26.9230769230769, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21355225, Length=658, Percent_Identity=23.5562310030395, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 71759; Mature: 71759

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAMPLWIPSAEQIEKAPMTAFIREAVHRTGRSIDGHAALHRWSVDHREEFWQLVWDFC
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
GIIGERGERVLIDGDKMPGAAYFPDAKLNFAENLLWKSGNGDAIVFRGEDKVERRISWHE
HHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHEECCCCCEEEECCCHHHHHHHHHHH
LKALVSRLQQLFKAEGVKAGDRIAGMMPNMPETVAAMLAAASLGAVWSSCSPDFGVRGVL
HHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DRFGQIEPVLFIAPDGYWYGGKAFDVGQKTAEILSALPSVRKALIVDYLGSARNAASGIE
HHCCCCCEEEEECCCCEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RAVALDDAIAPFSAGPLTYERLPFAHPLYILFSSGTTGIPKCIVHSAGGTLLQHVKEHRL
HHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHH
HAGLADGDRFFYFTTCGWMMWNWLVTGLASGATLLLYDGSPFYPDGNILFDFAAGERMTY
HCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCEEEE
FGTSAKFIDAVRKAGLEPARTHDLSSIRVISSTGSPLSPECFEFVYHSIKKDVHLASISG
ECCCHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCC
GTDIVSCFVLGIPLLPVWSGEIQGPGLGMAIEVWDDQGHPLESGKGELVCTKAFPSMPIA
CHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEE
FWNDPSGEKYRSAYFDRFENIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYN
ECCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCCCEEEECCCCCEECCCCEEECHHHHHH
QVEQLPEILEAICIGQDWDGDVRVVLFVRLAEGLMLDEALQKKIRDRIRTGASPRHVPAK
HHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
IVAVKDIPRTKSGKITELAVRDVVHGREVKNKEALANPEALELYRELPELKD
EEEEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKQAMPLWIPSAEQIEKAPMTAFIREAVHRTGRSIDGHAALHRWSVDHREEFWQLVWDFC
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
GIIGERGERVLIDGDKMPGAAYFPDAKLNFAENLLWKSGNGDAIVFRGEDKVERRISWHE
HHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHEECCCCCEEEECCCHHHHHHHHHHH
LKALVSRLQQLFKAEGVKAGDRIAGMMPNMPETVAAMLAAASLGAVWSSCSPDFGVRGVL
HHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DRFGQIEPVLFIAPDGYWYGGKAFDVGQKTAEILSALPSVRKALIVDYLGSARNAASGIE
HHCCCCCEEEEECCCCEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RAVALDDAIAPFSAGPLTYERLPFAHPLYILFSSGTTGIPKCIVHSAGGTLLQHVKEHRL
HHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHH
HAGLADGDRFFYFTTCGWMMWNWLVTGLASGATLLLYDGSPFYPDGNILFDFAAGERMTY
HCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCEEEE
FGTSAKFIDAVRKAGLEPARTHDLSSIRVISSTGSPLSPECFEFVYHSIKKDVHLASISG
ECCCHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCC
GTDIVSCFVLGIPLLPVWSGEIQGPGLGMAIEVWDDQGHPLESGKGELVCTKAFPSMPIA
CHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEE
FWNDPSGEKYRSAYFDRFENIWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYN
ECCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCCCEEEECCCCCEECCCCEEECHHHHHH
QVEQLPEILEAICIGQDWDGDVRVVLFVRLAEGLMLDEALQKKIRDRIRTGASPRHVPAK
HHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
IVAVKDIPRTKSGKITELAVRDVVHGREVKNKEALANPEALELYRELPELKD
EEEEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]