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The map label for this gene is puuA [H]

Identifier: 110632523

GI number: 110632523

Start: 197100

End: 198527

Strand: Reverse

Name: puuA [H]

Synonym: Meso_0161

Alternate gene names: 110632523

Gene position: 198527-197100 (Counterclockwise)

Preceding gene: 110632524

Following gene: 110632522

Centisome position: 4.5

GC content: 59.59

Gene sequence:

>1428_bases
ATGTTAGATGACAGCAAGGCAGGCCGCACCAACAGAGGCGGACGCCGGACGCCCGCTTTTTTGAAGAATTTGCGCGGAGT
TAAGGGCTGGAAAGAAGCGCGAGCCTGGCTCGAATGGCGCGGTATCGAGGATATCGAATGCATTGCGCCGGACCAAGCAG
GCGTCGCGCGCGGCAAGATGATGCCGTCGAAGAAGTTCATTTCGAACACGTCCCTCGCTCTTCCGTCCGCCGTTTTCATG
GCAACCATCTCCGGCGAATATCCTGAAGACGGCAACGGGTTCACCTATCCCGAGGACGACGGTGACCTGAAACTCGTGCC
GGATCTCTCTACCCTCTCAGTGGTTCCATGGGAGACGGACCCGACCGCCCAGGTGATCTGCGACCTGGCGCATCAGGACG
GGCGAGCGGTCGAATTCAGCCCGCGCAACGTCCTGAGGCGGGTCGTCGCCAAATATGCGGAGCACGGCCTGAAGCCGATT
GTCGCCCCTGAGATCGAATTCTATCTCGTTCACAAGAATCCTGATCCCGACTACCCGCTGACGCCTCCTGTCGGGCGTTC
GGGAAGGCCGATCGGTGGCGGACAGGGCTATTCGATCGCGGGTGTCAACGAGTTCGACGAACTCATTGACGACATCTACC
ACTTCTCCGAAGGCCAGGGGCTGGAGATCGATACGCTCATCCACGAAGAGGGCGCGGGGCAACTGGAGATCAATCTGCGC
CACGGCGACCCGGTTGAACTTGCCGATCAGGTCTTCCTCTTCAAGCGGACAATCCGGGAAGCCGCGCTCAAACACGAGAT
GTACGCCACCTTCATGGCCAAGCCGATCCAGGATCAGCCGGGCTCGGCGATGCATATCCATCAATCCGTGGTAACGTTGA
AGGGGGGGCGCAACGTCTTCTCCGATGAGAACGGCGAAGAAACAGACGTTTTTCGTCACTTCATCGGCGGCTTACAGCAG
CATATCCCGAACGCGCTGATCATGCTGGCGCCTTATGTGAACTCCTACAGGCGGCTGACACAGGCGGGCTCGGCGCCGGT
CAACACGCGCTGGGGTTATGACAACAGGACGACTGCCTTTCGCGTGCCCCGCTCGGATCCGAACTCCAGGCGCGTGGAAA
ACCGCATACCCTCCTCCGATGCCAATCCTTATTTGGCGCTTGCCGCCTCCCTGGCGTGCGGACTGATCGGAATACTCCAA
AAGATCGAACCCGACGCACCGGCCGGCACAGCGGTCAACACGGACGTGATCAACCTGCCCCGCGGTCTCCTCGAGGCGGT
CGCCCTGTTTGAAGCGGACGAGAGCCTCAGGAATATTGTCGGCGATCTTTTCGCGAGCACCTATGCCGCAATCAAGCGGG
CTGAATTCGAGACGTTCATGAAGGTCATCAGCCCTTGGGAGCGGGAGTATCTGTTGTTGAATGTGTGA

Upstream 100 bases:

>100_bases
AAGGACGGCGCAGGCCTGAAAGGGCCGTGCTCAATCTTGGTAACCCTTTCTTTCTAGGTTGTGCCAGGGCTATAGTGATC
AGAGCATTCTTGGTGAACCA

Downstream 100 bases:

>100_bases
TTAAGCGAGTAGCGAATAGGGTTTAGCGAGTAGGGATGACATTCTTAATCTTCCGCCAGCCCGCCATTCGCTATTCGCTA
CTCCCTATTCGCTGGTTGCC

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MLDDSKAGRTNRGGRRTPAFLKNLRGVKGWKEARAWLEWRGIEDIECIAPDQAGVARGKMMPSKKFISNTSLALPSAVFM
ATISGEYPEDGNGFTYPEDDGDLKLVPDLSTLSVVPWETDPTAQVICDLAHQDGRAVEFSPRNVLRRVVAKYAEHGLKPI
VAPEIEFYLVHKNPDPDYPLTPPVGRSGRPIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINLR
HGDPVELADQVFLFKRTIREAALKHEMYATFMAKPIQDQPGSAMHIHQSVVTLKGGRNVFSDENGEETDVFRHFIGGLQQ
HIPNALIMLAPYVNSYRRLTQAGSAPVNTRWGYDNRTTAFRVPRSDPNSRRVENRIPSSDANPYLALAASLACGLIGILQ
KIEPDAPAGTAVNTDVINLPRGLLEAVALFEADESLRNIVGDLFASTYAAIKRAEFETFMKVISPWEREYLLLNV

Sequences:

>Translated_475_residues
MLDDSKAGRTNRGGRRTPAFLKNLRGVKGWKEARAWLEWRGIEDIECIAPDQAGVARGKMMPSKKFISNTSLALPSAVFM
ATISGEYPEDGNGFTYPEDDGDLKLVPDLSTLSVVPWETDPTAQVICDLAHQDGRAVEFSPRNVLRRVVAKYAEHGLKPI
VAPEIEFYLVHKNPDPDYPLTPPVGRSGRPIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINLR
HGDPVELADQVFLFKRTIREAALKHEMYATFMAKPIQDQPGSAMHIHQSVVTLKGGRNVFSDENGEETDVFRHFIGGLQQ
HIPNALIMLAPYVNSYRRLTQAGSAPVNTRWGYDNRTTAFRVPRSDPNSRRVENRIPSSDANPYLALAASLACGLIGILQ
KIEPDAPAGTAVNTDVINLPRGLLEAVALFEADESLRNIVGDLFASTYAAIKRAEFETFMKVISPWEREYLLLNV
>Mature_475_residues
MLDDSKAGRTNRGGRRTPAFLKNLRGVKGWKEARAWLEWRGIEDIECIAPDQAGVARGKMMPSKKFISNTSLALPSAVFM
ATISGEYPEDGNGFTYPEDDGDLKLVPDLSTLSVVPWETDPTAQVICDLAHQDGRAVEFSPRNVLRRVVAKYAEHGLKPI
VAPEIEFYLVHKNPDPDYPLTPPVGRSGRPIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINLR
HGDPVELADQVFLFKRTIREAALKHEMYATFMAKPIQDQPGSAMHIHQSVVTLKGGRNVFSDENGEETDVFRHFIGGLQQ
HIPNALIMLAPYVNSYRRLTQAGSAPVNTRWGYDNRTTAFRVPRSDPNSRRVENRIPSSDANPYLALAASLACGLIGILQ
KIEPDAPAGTAVNTDVINLPRGLLEAVALFEADESLRNIVGDLFASTYAAIKRAEFETFMKVISPWEREYLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=426, Percent_Identity=26.056338028169, Blast_Score=135, Evalue=9e-32,
Organism=Escherichia coli, GI87081870, Length=462, Percent_Identity=33.7662337662338, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1790301, Length=350, Percent_Identity=29.4285714285714, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 52522; Mature: 52522

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDSKAGRTNRGGRRTPAFLKNLRGVKGWKEARAWLEWRGIEDIECIAPDQAGVARGKM
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHCCC
MPSKKFISNTSLALPSAVFMATISGEYPEDGNGFTYPEDDGDLKLVPDLSTLSVVPWETD
CCCHHHHCCCCHHCCHHHHHEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCC
PTAQVICDLAHQDGRAVEFSPRNVLRRVVAKYAEHGLKPIVAPEIEFYLVHKNPDPDYPL
CHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCC
TPPVGRSGRPIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINLR
CCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEC
HGDPVELADQVFLFKRTIREAALKHEMYATFMAKPIQDQPGSAMHIHQSVVTLKGGRNVF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHEEEEEECCCCCC
SDENGEETDVFRHFIGGLQQHIPNALIMLAPYVNSYRRLTQAGSAPVNTRWGYDNRTTAF
CCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
RVPRSDPNSRRVENRIPSSDANPYLALAASLACGLIGILQKIEPDAPAGTAVNTDVINLP
ECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCC
RGLLEAVALFEADESLRNIVGDLFASTYAAIKRAEFETFMKVISPWEREYLLLNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MLDDSKAGRTNRGGRRTPAFLKNLRGVKGWKEARAWLEWRGIEDIECIAPDQAGVARGKM
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHCCC
MPSKKFISNTSLALPSAVFMATISGEYPEDGNGFTYPEDDGDLKLVPDLSTLSVVPWETD
CCCHHHHCCCCHHCCHHHHHEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCC
PTAQVICDLAHQDGRAVEFSPRNVLRRVVAKYAEHGLKPIVAPEIEFYLVHKNPDPDYPL
CHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCC
TPPVGRSGRPIGGGQGYSIAGVNEFDELIDDIYHFSEGQGLEIDTLIHEEGAGQLEINLR
CCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEC
HGDPVELADQVFLFKRTIREAALKHEMYATFMAKPIQDQPGSAMHIHQSVVTLKGGRNVF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHEEEEEECCCCCC
SDENGEETDVFRHFIGGLQQHIPNALIMLAPYVNSYRRLTQAGSAPVNTRWGYDNRTTAF
CCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
RVPRSDPNSRRVENRIPSSDANPYLALAASLACGLIGILQKIEPDAPAGTAVNTDVINLP
ECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCC
RGLLEAVALFEADESLRNIVGDLFASTYAAIKRAEFETFMKVISPWEREYLLLNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]