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The map label for this gene is bsp6IM [H]

Identifier: 109947509

GI number: 109947509

Start: 846004

End: 846537

Strand: Reverse

Name: bsp6IM [H]

Synonym: Hac_0966

Alternate gene names: 109947509

Gene position: 846537-846004 (Counterclockwise)

Preceding gene: 109947510

Following gene: 109947502

Centisome position: 54.48

GC content: 38.95

Gene sequence:

>534_bases
ATGGAAGTAGGCTCACTTTTTGCTGGGATTGGAGGGTTTGAATGCATGTTTTTGCAAGCAGGCTTTGAAATTGGCTGGGC
GAATGAATTGGACAAAGACGCATGCAACACTTATAGGGCTAATTTCAAACACAAGCTTTTAGAACAAGACATTAAAGATT
TAAACCCTAATGAATTAGAAGATGTTGGGTTAATAAGTGCAGGGTTTCCTTGTCAAGCGTTTAGCATTGCTGGGTTACAA
AAAGGGCTTAATGATGAAAAAGGGGCGATCGTTATGGAAATGTTTTGCATCATTCAAGCTAAAAAACCTCATGTTTTATT
ATTGGAAAATGTAAAAAATTTAGCGAGCCACAACAAAGGGAAAACCTTACTAAAATCCGCTCCCCTTATTCAAATCGCCA
CTAAGCAGATCGCCTCAAATCCCCATCAACCCCCTAAAATAGGGCATATAGCGTTTTTACGCATTATAAGGCATCATACA
GCGAAACAATGCCAAAAGAATACCGAGAGTACACCCCATATCGTTGAAATTTGA

Upstream 100 bases:

>100_bases
AAAAGCAAAAAGAAGGCAAGAAACGCATGAAAGCTATCGGTAAGGTAGAGCTTCCCCAAGATGTATTTTTGGCGATTTTA
AAGATTGATTAGGGTGTTTG

Downstream 100 bases:

>100_bases
CCACTTTACGGCTCTTTAAACGCTATGTTTAGTTTTGAGGGTTGGAAAAGTCTGTTTTTGAGCGACCGCAATTGGGTTAA
AAAATGACTTTTAATTTAAA

Product: methylase

Products: NA

Alternate protein names: M.Bsp6I; Cytosine-specific methyltransferase Bsp6I [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MEVGSLFAGIGGFECMFLQAGFEIGWANELDKDACNTYRANFKHKLLEQDIKDLNPNELEDVGLISAGFPCQAFSIAGLQ
KGLNDEKGAIVMEMFCIIQAKKPHVLLLENVKNLASHNKGKTLLKSAPLIQIATKQIASNPHQPPKIGHIAFLRIIRHHT
AKQCQKNTESTPHIVEI

Sequences:

>Translated_177_residues
MEVGSLFAGIGGFECMFLQAGFEIGWANELDKDACNTYRANFKHKLLEQDIKDLNPNELEDVGLISAGFPCQAFSIAGLQ
KGLNDEKGAIVMEMFCIIQAKKPHVLLLENVKNLASHNKGKTLLKSAPLIQIATKQIASNPHQPPKIGHIAFLRIIRHHT
AKQCQKNTESTPHIVEI
>Mature_177_residues
MEVGSLFAGIGGFECMFLQAGFEIGWANELDKDACNTYRANFKHKLLEQDIKDLNPNELEDVGLISAGFPCQAFSIAGLQ
KGLNDEKGAIVMEMFCIIQAKKPHVLLLENVKNLASHNKGKTLLKSAPLIQIATKQIASNPHQPPKIGHIAFLRIIRHHT
AKQCQKNTESTPHIVEI

Specific function: This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=149, Percent_Identity=34.8993288590604, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 19553; Mature: 19553

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVGSLFAGIGGFECMFLQAGFEIGWANELDKDACNTYRANFKHKLLEQDIKDLNPNELE
CCCHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DVGLISAGFPCQAFSIAGLQKGLNDEKGAIVMEMFCIIQAKKPHVLLLENVKNLASHNKG
HCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCC
KTLLKSAPLIQIATKQIASNPHQPPKIGHIAFLRIIRHHTAKQCQKNTESTPHIVEI
CHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
>Mature Secondary Structure
MEVGSLFAGIGGFECMFLQAGFEIGWANELDKDACNTYRANFKHKLLEQDIKDLNPNELE
CCCHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DVGLISAGFPCQAFSIAGLQKGLNDEKGAIVMEMFCIIQAKKPHVLLLENVKNLASHNKG
HCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCC
KTLLKSAPLIQIATKQIASNPHQPPKIGHIAFLRIIRHHTAKQCQKNTESTPHIVEI
CHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607501 [H]