Click here to switch to the map view.

The map label for this gene is amiE

Identifier: 109947145

GI number: 109947145

Start: 486213

End: 487232

Strand: Direct

Name: amiE

Synonym: Hac_0554

Alternate gene names: 109947145

Gene position: 486213-487232 (Clockwise)

Preceding gene: 109947144

Following gene: 109947147

Centisome position: 31.29

GC content: 43.63

Gene sequence:

>1020_bases
ATGAGACATGGAGATATTAGTAGCAGTCCAGATACTGTGGGTGTAGCGGTAGTCAATTATAAGATGCCTAGGCTTCACAC
TAAAGAACAGGTGTTAGAAAATTGCCGTAATATCGCTAAGGTGATTGGTGGGGTTAAACAAGGTCTGCCAGGATTGGATC
TCATTATTTTCCCTGAATACAGCACGCATGGGATTATGTATGACAAACAAGAGATGTTTGACACAGCTGTGAGTATTCCT
GGAGAAGAAACAGCGATACTTGCTGAGGCTTGTAAGAAAAATAAGGTTTGGGGCGTGTTCTCTTTAACTGGGGAAAAACA
CGAGCAAGCTAAAAAAAATCCCTATAACACTTTGATTCTTGTCAATGATAAGGGTGAGATTGTGCAAAAATACCGCAAAA
TCTTACCTTGGTGTCCTATTGAATGTTGGTATCCTGGGGATAAAACTTATGTGGTTGATGGGCCTAAGGGCTTGAAAGTT
TCTTTGATTATTTGCGATGATGGTAACTACCCTGAGATTTGGCGCGATTGTGCGATGCGTGGGGCAGAACTCATTGTGCG
CTGTCAAGGCTATATGTATCCAGCTAAAGAGCAACAAATTGCGATAGTGAAAGCTATGGCGTGGGCTAATCAATGTTATG
TAGCCGTGGCGAATGCGACCGGTTTTGATGGGGTGTATTCCTATTTTGGGCATTCTAGCATTATTGGTTTTGACGGGCAC
ACTTTGGGCGAATGCGGAGAAGAAGAAAATGGGATTCAATACGCTCAGCTCTCTGTGCAACAAATCCGTGATGCGAGAAA
ATACGACCAAAGCCAAAACCAGCTCTTCAAACTCTTACACAGAGGTTATAGTGGGGTTTTTGCTAGTGGTGATGGGGATA
AGGGTGTGGCGGAATGCCCTTTTGAGTTCTACAAAACTTGGGTTAATGACCCCAAAAAAGCTCAAGAAAATGTAGAAAAA
TTCACTCGCCCAAGCGTGGGTGTGGCCGCTTGTCCTGTAGGCGATTTGCCCACGAAATAA

Upstream 100 bases:

>100_bases
TTAAAGTTTTTATATTCATTATAAATCCATTTACACAATTATTTTATAAATTCAAACAGAGAGTTTGTAGGAACTCTCAT
CAAAAAACAAGGAGCATAAC

Downstream 100 bases:

>100_bases
AGGATAAGGGAGGGGGTTAAAAGTCTTAAAAATATTTCTCTTTAACATCTCTCACTAGAACGCTAAACACACTAAAAGGT
TACAAATATTTCGTTAAAGA

Product: acylamide amidohydrolase

Products: NA

Alternate protein names: Acylamide amidohydrolase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRHGDISSSPDTVGVAVVNYKMPRLHTKEQVLENCRNIAKVIGGVKQGLPGLDLIIFPEYSTHGIMYDKQEMFDTAVSIP
GEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLILVNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKV
SLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
TLGECGEEENGIQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECPFEFYKTWVNDPKKAQENVEK
FTRPSVGVAACPVGDLPTK

Sequences:

>Translated_339_residues
MRHGDISSSPDTVGVAVVNYKMPRLHTKEQVLENCRNIAKVIGGVKQGLPGLDLIIFPEYSTHGIMYDKQEMFDTAVSIP
GEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLILVNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKV
SLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
TLGECGEEENGIQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECPFEFYKTWVNDPKKAQENVEK
FTRPSVGVAACPVGDLPTK
>Mature_339_residues
MRHGDISSSPDTVGVAVVNYKMPRLHTKEQVLENCRNIAKVIGGVKQGLPGLDLIIFPEYSTHGIMYDKQEMFDTAVSIP
GEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLILVNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKV
SLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
TLGECGEEENGIQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECPFEFYKTWVNDPKKAQENVEK
FTRPSVGVAACPVGDLPTK

Specific function: Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Saccharomyces cerevisiae, GI6323383, Length=256, Percent_Identity=24.21875, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMIE_HELAH (Q17YA2)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664373.1
- ProteinModelPortal:   Q17YA2
- SMR:   Q17YA2
- STRING:   Q17YA2
- GeneID:   4177703
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0554
- NMPDR:   fig|382638.8.peg.538
- eggNOG:   COG0388
- HOGENOM:   HBG287568
- OMA:   QNHLFKL
- ProtClustDB:   PRK13286
- BioCyc:   HACI382638:HAC_0554-MONOMER
- HAMAP:   MF_01242
- InterPro:   IPR003010
- Gene3D:   G3DSA:3.60.110.10

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: =3.5.1.4

Molecular weight: Translated: 37741; Mature: 37741

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50263 CN_HYDROLASE

Important sites: ACT_SITE 59-59 ACT_SITE 133-133 ACT_SITE 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHGDISSSPDTVGVAVVNYKMPRLHTKEQVLENCRNIAKVIGGVKQGLPGLDLIIFPEY
CCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
STHGIMYDKQEMFDTAVSIPGEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLIL
CCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCEEEE
VNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAMR
ECCCHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHC
GAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
CHHEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCEEEECCCC
TLGECGEEENGIQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEECCCCCCCHHHCC
FEFYKTWVNDPKKAQENVEKFTRPSVGVAACPVGDLPTK
HHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MRHGDISSSPDTVGVAVVNYKMPRLHTKEQVLENCRNIAKVIGGVKQGLPGLDLIIFPEY
CCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
STHGIMYDKQEMFDTAVSIPGEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLIL
CCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCEEEE
VNDKGEIVQKYRKILPWCPIECWYPGDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAMR
ECCCHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHC
GAELIVRCQGYMYPAKEQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGH
CHHEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCEEEECCCC
TLGECGEEENGIQYAQLSVQQIRDARKYDQSQNQLFKLLHRGYSGVFASGDGDKGVAECP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEECCCCCCCHHHCC
FEFYKTWVNDPKKAQENVEKFTRPSVGVAACPVGDLPTK
HHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA