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The map label for this gene is hpaim [H]

Identifier: 109947114

GI number: 109947114

Start: 446706

End: 447464

Strand: Direct

Name: hpaim [H]

Synonym: Hac_0520

Alternate gene names: 109947114

Gene position: 446706-447464 (Clockwise)

Preceding gene: 109947113

Following gene: 109947115

Centisome position: 28.75

GC content: 38.08

Gene sequence:

>759_bases
ATGAAACCTTATTTTAGTTTAGATAAATTGCATTTATACCATGGCGATGCGAGCGTTTTAGAGACTTTTGAAAAAGGTTT
TTATGATTTGTGTGTTACTTCACCGCCCTATAATTTGAGTGTTGAATATCAAGGGAGTAACGATTTTAGGGCTTATGATG
ATTATTTGAATTGGTGTAAAAATTGGTTTAAAAATTGTTATTTTTGGGGTAAGGAGCAAGCGAGATTGTGCTTAAATGTC
CCTTTAGACACGAATAAACACGGCAAGCAAAGTTTGGGGGCAGATATGACTATAGTGGCTAAAGAATGCGGTTGGAAATA
CCAAAATACGATCATTTGGAATGAAAGCAATATTTCAAGACGCACCGCTTGGGGGAGTTGGTTGCAAGCTAGCGCGCCTT
ATGCTATCGCTCCTGTGGAATTAATCATTGTTTTTTATAAAAATGAATACAAACGCCAAAAACAAACTTCTACGATCAGC
AAAGAGGAATTTTTACTCTACACGAATGGGCTTTGGAGTTTTAGCGGCGAATCCAAAAAACGCTTAAAACACCCAGCCCC
ATTCCCAAGGGAATTACCCAGTCGTTGCATTAAATTGTTTTCTTTTTTGGAAGACACGATTTTTGATCCTTTTAGCGGCT
CTGGCACCACCCTCTTAGAGGCAAATGCTTTAGGGCGTTTTAGCGTGGGATTAGAAATTGAAAAAGAATATTGCGAGTTG
TCTAAAAAGCGTATTTTAGAGAGTTTGTCACTAGTGTGA

Upstream 100 bases:

>100_bases
AGCATGAATTAGATGGCATCTATTTAGCCCAAAGTGGTTTTGATAAAAGTTTTAAAATCAAGGGCATAGAAAATTTACTA
GAAGACTTAAAAAGGCTTTT

Downstream 100 bases:

>100_bases
GCCTCTTAAAAACCTTTGAGAGCGAAAATAGTGTAAAATAGTAAAAATTTTTAAAACTCAAAAAGGATTGATAATGAATT
TATTTGAAAAAATGACCAAC

Product: adenine specific DNA methyltransferase

Products: NA

Alternate protein names: M.MjaVI; N-4 cytosine-specific methyltransferase MjaVI [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKPYFSLDKLHLYHGDASVLETFEKGFYDLCVTSPPYNLSVEYQGSNDFRAYDDYLNWCKNWFKNCYFWGKEQARLCLNV
PLDTNKHGKQSLGADMTIVAKECGWKYQNTIIWNESNISRRTAWGSWLQASAPYAIAPVELIIVFYKNEYKRQKQTSTIS
KEEFLLYTNGLWSFSGESKKRLKHPAPFPRELPSRCIKLFSFLEDTIFDPFSGSGTTLLEANALGRFSVGLEIEKEYCEL
SKKRILESLSLV

Sequences:

>Translated_252_residues
MKPYFSLDKLHLYHGDASVLETFEKGFYDLCVTSPPYNLSVEYQGSNDFRAYDDYLNWCKNWFKNCYFWGKEQARLCLNV
PLDTNKHGKQSLGADMTIVAKECGWKYQNTIIWNESNISRRTAWGSWLQASAPYAIAPVELIIVFYKNEYKRQKQTSTIS
KEEFLLYTNGLWSFSGESKKRLKHPAPFPRELPSRCIKLFSFLEDTIFDPFSGSGTTLLEANALGRFSVGLEIEKEYCEL
SKKRILESLSLV
>Mature_252_residues
MKPYFSLDKLHLYHGDASVLETFEKGFYDLCVTSPPYNLSVEYQGSNDFRAYDDYLNWCKNWFKNCYFWGKEQARLCLNV
PLDTNKHGKQSLGADMTIVAKECGWKYQNTIIWNESNISRRTAWGSWLQASAPYAIAPVELIIVFYKNEYKRQKQTSTIS
KEEFLLYTNGLWSFSGESKKRLKHPAPFPRELPSRCIKLFSFLEDTIFDPFSGSGTTLLEANALGRFSVGLEIEKEYCEL
SKKRILESLSLV

Specific function: This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the MjaVI endonuclease (Potential) [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 29196; Mature: 29196

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYFSLDKLHLYHGDASVLETFEKGFYDLCVTSPPYNLSVEYQGSNDFRAYDDYLNWCK
CCCCCCCCEEEEEECCHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHH
NWFKNCYFWGKEQARLCLNVPLDTNKHGKQSLGADMTIVAKECGWKYQNTIIWNESNISR
HHHHHCEECCCCCCEEEEECCCCCCCCCHHHHCCCEEEEEHHCCCEECCEEEECCCCCCH
RTAWGSWLQASAPYAIAPVELIIVFYKNEYKRQKQTSTISKEEFLLYTNGLWSFSGESKK
HHHHHHHHHCCCCEEECCEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCEEECCCCHHH
RLKHPAPFPRELPSRCIKLFSFLEDTIFDPFSGSGTTLLEANALGRFSVGLEIEKEYCEL
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECEEECHHHHHH
SKKRILESLSLV
HHHHHHHHHCCC
>Mature Secondary Structure
MKPYFSLDKLHLYHGDASVLETFEKGFYDLCVTSPPYNLSVEYQGSNDFRAYDDYLNWCK
CCCCCCCCEEEEEECCHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHH
NWFKNCYFWGKEQARLCLNVPLDTNKHGKQSLGADMTIVAKECGWKYQNTIIWNESNISR
HHHHHCEECCCCCCEEEEECCCCCCCCCHHHHCCCEEEEEHHCCCEECCEEEECCCCCCH
RTAWGSWLQASAPYAIAPVELIIVFYKNEYKRQKQTSTISKEEFLLYTNGLWSFSGESKK
HHHHHHHHHCCCCEEECCEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCEEECCCCHHH
RLKHPAPFPRELPSRCIKLFSFLEDTIFDPFSGSGTTLLEANALGRFSVGLEIEKEYCEL
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECEEECHHHHHH
SKKRILESLSLV
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]