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The map label for this gene is gdhA [H]

Identifier: 109947074

GI number: 109947074

Start: 422135

End: 423481

Strand: Direct

Name: gdhA [H]

Synonym: Hac_0475

Alternate gene names: 109947074

Gene position: 422135-423481 (Clockwise)

Preceding gene: 109947073

Following gene: 109947088

Centisome position: 27.17

GC content: 41.43

Gene sequence:

>1347_bases
ATGTATATTGAAAAAATCCTCCAATCTTTACAAAAAAAATACCCTTATCAAAAAGAGTTCCATCAAGCTGTCTATGAAGC
TATCACTTCGTTAAAACCCCTTTTAGACAGCGATAAAAGTTACCAAAAGCATGCGATTTTAGAGCGTTTGATTGAGCCTG
AAAGAGAGATTTTTTTTAGAGTGTGTTGGCTAGATGATAATAATCAAATCCAAGTCAATTGGGGGTGTAGGGTTGAATTC
AATTCGGCTATTGGTCCTTATAAAGGGGGTTTGAGATTCCATCCTAGCGTGAATGAAAGCGTGATCAAATTCTTAGGCTT
TGAGCAAGTGTTAAAAAATTCGCTTACTACTTTGGCTATGGGGGGTGCTAAGGGGGGGAGCGATTTTGACCCTAAGGGAA
AGAGCGAGCATGAAATCATGCGTTTTTGTCAAGCGTTCATGAATGAATTATACCGCCATATTGGAGCCACAACTGATGTG
CCAGCTGGGGATATTGGGGTGGGTGAAAGAGAGATTGGCTATTTGTTTGGGCAATACAAGAAATTAGTCAATCGTTTTGA
GGGCGTATTGACCGGTAAGGGGCTTACTTATGGGGGGAGTTTATGCCGAAAAGAATCTACCGGTTATGGGTGCGTGTATT
TTGCTGAAGAAATGTTACAGGAAAGAAACAGCTCTTTAGAGGGTAAGATTTGCAGCGTTTCTGGGAGCGGTAATGTCGCT
ATTTATACGATTGAAAAATTGCTTCAAATAGGTGCTAAACCGGTAACTGCAAGCGATTCTAATGGCATGATTTATGATAA
AGAGGGCATTGATTTAGATCTTTTAAAAGAGATTAAAGAAGTGCGTCGTGGGAGGATTAAAGAATACGCTTTAGAAAAAA
CAAGCGCGAAATACACGCCTGTAGAAAACTACCCCAAAGGGGGGAATGCAATATGGCATGTGCCTTGTTTTGCAGCTTTT
CCTAGCGCGACAGAGAATGAATTGAGCGTTTTAGACGCCAAAACCCTCCTTTCTAACGGATGTAAATGCGTGGTTGAAGG
GGCAAACATGCCCTCAAGCAATGAAGCGGTTGAATTGTTTTTGCAGGCTAAGATTTCTTATGGTTTAGGCAAGGCGGCTA
ATGCTGGGGGGGTGAGCGTGAGCGGTTTGGAAATGGCACAAAATGCGAGCATGCACCCATGGAGTTTTGAAGTGGTGGAT
GCAAAATTACACCATATTATGAAAGAGATTTATAAAAATGTCTCTCAAACCGCTAAAGAGTTTAAAGACCCTACTAATTT
TGTTTTAGGGGCTAATATCGCTGGTTTTAGAAAGGTAGCGTCTGCGATGATAGCCCAAGGGGTTTAA

Upstream 100 bases:

>100_bases
TTTAACTATCCCTTTTGATGAGAAAAGCCAAAAGAATTAAAACTTAATTACCCTTTTAGTGTTACAATAAAAACCTAAAA
ACAATAAAGGAATGCCGCTC

Downstream 100 bases:

>100_bases
AGGGTTGTTTAAAAACCCTATTTTAAGAAAGTCTCTAGTAATCGCTTGAAAAAGCCTAAATTTAAAAAGACTAAAAGGCG
TTTTTTAAAGGGTGTTTTAC

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYQKHAILERLIEPEREIFFRVCWLDDNNQIQVNWGCRVEF
NSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAMGGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDV
PAGDIGVGEREIGYLFGQYKKLVNRFEGVLTGKGLTYGGSLCRKESTGYGCVYFAEEMLQERNSSLEGKICSVSGSGNVA
IYTIEKLLQIGAKPVTASDSNGMIYDKEGIDLDLLKEIKEVRRGRIKEYALEKTSAKYTPVENYPKGGNAIWHVPCFAAF
PSATENELSVLDAKTLLSNGCKCVVEGANMPSSNEAVELFLQAKISYGLGKAANAGGVSVSGLEMAQNASMHPWSFEVVD
AKLHHIMKEIYKNVSQTAKEFKDPTNFVLGANIAGFRKVASAMIAQGV

Sequences:

>Translated_448_residues
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYQKHAILERLIEPEREIFFRVCWLDDNNQIQVNWGCRVEF
NSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAMGGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDV
PAGDIGVGEREIGYLFGQYKKLVNRFEGVLTGKGLTYGGSLCRKESTGYGCVYFAEEMLQERNSSLEGKICSVSGSGNVA
IYTIEKLLQIGAKPVTASDSNGMIYDKEGIDLDLLKEIKEVRRGRIKEYALEKTSAKYTPVENYPKGGNAIWHVPCFAAF
PSATENELSVLDAKTLLSNGCKCVVEGANMPSSNEAVELFLQAKISYGLGKAANAGGVSVSGLEMAQNASMHPWSFEVVD
AKLHHIMKEIYKNVSQTAKEFKDPTNFVLGANIAGFRKVASAMIAQGV
>Mature_448_residues
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYQKHAILERLIEPEREIFFRVCWLDDNNQIQVNWGCRVEF
NSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAMGGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDV
PAGDIGVGEREIGYLFGQYKKLVNRFEGVLTGKGLTYGGSLCRKESTGYGCVYFAEEMLQERNSSLEGKICSVSGSGNVA
IYTIEKLLQIGAKPVTASDSNGMIYDKEGIDLDLLKEIKEVRRGRIKEYALEKTSAKYTPVENYPKGGNAIWHVPCFAAF
PSATENELSVLDAKTLLSNGCKCVVEGANMPSSNEAVELFLQAKISYGLGKAANAGGVSVSGLEMAQNASMHPWSFEVVD
AKLHHIMKEIYKNVSQTAKEFKDPTNFVLGANIAGFRKVASAMIAQGV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=337, Percent_Identity=29.673590504451, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4885281, Length=337, Percent_Identity=29.3768545994065, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1788059, Length=449, Percent_Identity=57.4610244988864, Blast_Score=503, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544676, Length=338, Percent_Identity=26.6272189349112, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=47.3568281938326, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6324951, Length=454, Percent_Identity=47.136563876652, Blast_Score=350, Evalue=2e-97,
Organism=Drosophila melanogaster, GI45549226, Length=407, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45553475, Length=376, Percent_Identity=28.7234042553192, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24649460, Length=324, Percent_Identity=28.0864197530864, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24649283, Length=320, Percent_Identity=27.5, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 49523; Mature: 49523

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYQKHAILERLIEPEREIFFR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHEEEE
VCWLDDNNQIQVNWGCRVEFNSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAM
EEEECCCCEEEEEECEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHEEE
GGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDVPAGDIGVGEREIGYLFGQYK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
KLVNRFEGVLTGKGLTYGGSLCRKESTGYGCVYFAEEMLQERNSSLEGKICSVSGSGNVA
HHHHHHHHEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEE
IYTIEKLLQIGAKPVTASDSNGMIYDKEGIDLDLLKEIKEVRRGRIKEYALEKTSAKYTP
EEEHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VENYPKGGNAIWHVPCFAAFPSATENELSVLDAKTLLSNGCKCVVEGANMPSSNEAVELF
CCCCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHH
LQAKISYGLGKAANAGGVSVSGLEMAQNASMHPWSFEVVDAKLHHIMKEIYKNVSQTAKE
HHHHHHHCCCCCCCCCCCEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKDPTNFVLGANIAGFRKVASAMIAQGV
HCCCCCEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYQKHAILERLIEPEREIFFR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHEEEE
VCWLDDNNQIQVNWGCRVEFNSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAM
EEEECCCCEEEEEECEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHEEE
GGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDVPAGDIGVGEREIGYLFGQYK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
KLVNRFEGVLTGKGLTYGGSLCRKESTGYGCVYFAEEMLQERNSSLEGKICSVSGSGNVA
HHHHHHHHEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEE
IYTIEKLLQIGAKPVTASDSNGMIYDKEGIDLDLLKEIKEVRRGRIKEYALEKTSAKYTP
EEEHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VENYPKGGNAIWHVPCFAAFPSATENELSVLDAKTLLSNGCKCVVEGANMPSSNEAVELF
CCCCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHH
LQAKISYGLGKAANAGGVSVSGLEMAQNASMHPWSFEVVDAKLHHIMKEIYKNVSQTAKE
HHHHHHHCCCCCCCCCCCEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKDPTNFVLGANIAGFRKVASAMIAQGV
HCCCCCEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]