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The map label for this gene is putP [H]

Identifier: 108812431

GI number: 108812431

Start: 2551264

End: 2552748

Strand: Reverse

Name: putP [H]

Synonym: YPN_2270

Alternate gene names: 108812431

Gene position: 2552748-2551264 (Counterclockwise)

Preceding gene: 108812432

Following gene: 108812430

Centisome position: 56.3

GC content: 48.82

Gene sequence:

>1485_bases
ATGACCATGAATACGCCGATGCTGGTGACATTTTTAGTTTACATTTTGGGGATGATATTAATCGGGTTGATCGCCTATCG
GGCGACCAATAACTTCGATGACTACATCTTGGGTGGCCGGCGTTTAGGCAGTGTCGTCACCGCGTTATCCGCGGGTGCTT
CCGATATGAGTGGCTGGTTATTAATGGGGTTACCGGGTGCGGTTTTTTTGTCCGGTATTTCCGAAAGTTGGATAGCCATA
GGCTTGACAATCGGTGCTTATCTTAATTGGAAATGGGTGGCAGGCCGTTTACGGGTTCATACCGAAGCCAATAATAATGC
CCTGACATTACCCGATTATTTCACCAGTCGCTTTGAAGACAAGAGTAAATTACTGCGGGTCATATCCGCTGTGGTTATCT
TAGTCTTCTTTACTATTTACTGTGCTTCAGGGATTGTGGCCGGTGCGCGTTTGTTCGAAAGCACTTTCGGTATGGACTAC
GGAACCGCATTATGGGCAGGGGCCGCCGCGACCATCATCTATACCTTTATTGGGGGGTTCCTGGCCGTAAGCTGGACAGA
TACTGTACAGGCGACGTTAATGATTTTCGCCCTGATCCTGACGCCAATTATCGTGATTCTTGCTGTTGGGGGGATAGATA
CCTCAATGATGGTGATCGCGGCGAAAAACCCCGCAAATATTGATATGTTCAAAGGGTTGAATCTGGTGGCGATCCTCTCG
CTGTTGGGATGGGGGTTGGGCTACTTTGGTCAGCCACATATTTTGGCTCGCTTCATGGCGGCAGACTCTCATCGTACTAT
CCGTAGTGCCCGCCGTATTAGTATGACCTGGATGATCCTTTGTCTGGCGGGGACTATCGCAGTAGGCTTCTTTGGCATTG
CTTACTTTGAAAATAACCCTGAGCAAGCAGGGAATGTGAGCCAAAATGGTGAGCGGGTATTTATCGAATTAGCTAAGCTG
CTGTTCAACCCGTGGATTGCCGGTATTCTGTTGTCAGCCATTCTGGCTGCGGTTATGAGTACCTTGAGTTGTCAATTATT
GGTGTGCTCTAGCGCACTGACCGAAGATTTATATAAAGCATTCTTGCGTAAAAAAGCCAGCCAAAAAGAACTCGTCTGGG
TGGGCCGTGCCATGGTGTTATTAGTTGCATTGATAGCTATTGCACTGGCGGCAGATCCTGATAATCGGGTCCTCGGATTA
GTCAGCTATGCATGGGCAGGCTTCGGTGCAGCTTTTGGGCCAGTGGTCCTTATCTCGGTGCTGTGGCCACGAATGACACG
TAATGGTGCGTTGGTGGGAATGCTGGTCGGTGCCATCACGGTGCTTGTCTGGAAACAGTATGGCTGGTTAGATCTGTATG
AAATTATTCCAGGCTTCTTGTTTGCCAGTTTGGCTATTTTTGTCGTCAGCCTGATGGGCCGCGAGCCAAGTAATGCCATC
ACCGAACGTTTCCATAAAGCCGAGGCGGAGTTTAAAACAGTTTAA

Upstream 100 bases:

>100_bases
AAAGCGTAGAGAAGCAAGCGCTGAATGTCTTAAAGATGACGAAATGTATTAAAGATGGCGAAATATATTAAAACGATAAT
ATGCACAATGGAGACGTAGC

Downstream 100 bases:

>100_bases
TCGTCTGGCGTTCTAAACCTATCAATACGTTTAACTACACATAGCCTCCTGCCATAACAGGGGGCTATCTTGTATGTAAA
ATCATCTTTGTATGTAAAAA

Product: proline permease

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTMNTPMLVTFLVYILGMILIGLIAYRATNNFDDYILGGRRLGSVVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAI
GLTIGAYLNWKWVAGRLRVHTEANNNALTLPDYFTSRFEDKSKLLRVISAVVILVFFTIYCASGIVAGARLFESTFGMDY
GTALWAGAAATIIYTFIGGFLAVSWTDTVQATLMIFALILTPIIVILAVGGIDTSMMVIAAKNPANIDMFKGLNLVAILS
LLGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFENNPEQAGNVSQNGERVFIELAKL
LFNPWIAGILLSAILAAVMSTLSCQLLVCSSALTEDLYKAFLRKKASQKELVWVGRAMVLLVALIAIALAADPDNRVLGL
VSYAWAGFGAAFGPVVLISVLWPRMTRNGALVGMLVGAITVLVWKQYGWLDLYEIIPGFLFASLAIFVVSLMGREPSNAI
TERFHKAEAEFKTV

Sequences:

>Translated_494_residues
MTMNTPMLVTFLVYILGMILIGLIAYRATNNFDDYILGGRRLGSVVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAI
GLTIGAYLNWKWVAGRLRVHTEANNNALTLPDYFTSRFEDKSKLLRVISAVVILVFFTIYCASGIVAGARLFESTFGMDY
GTALWAGAAATIIYTFIGGFLAVSWTDTVQATLMIFALILTPIIVILAVGGIDTSMMVIAAKNPANIDMFKGLNLVAILS
LLGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFENNPEQAGNVSQNGERVFIELAKL
LFNPWIAGILLSAILAAVMSTLSCQLLVCSSALTEDLYKAFLRKKASQKELVWVGRAMVLLVALIAIALAADPDNRVLGL
VSYAWAGFGAAFGPVVLISVLWPRMTRNGALVGMLVGAITVLVWKQYGWLDLYEIIPGFLFASLAIFVVSLMGREPSNAI
TERFHKAEAEFKTV
>Mature_493_residues
TMNTPMLVTFLVYILGMILIGLIAYRATNNFDDYILGGRRLGSVVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIG
LTIGAYLNWKWVAGRLRVHTEANNNALTLPDYFTSRFEDKSKLLRVISAVVILVFFTIYCASGIVAGARLFESTFGMDYG
TALWAGAAATIIYTFIGGFLAVSWTDTVQATLMIFALILTPIIVILAVGGIDTSMMVIAAKNPANIDMFKGLNLVAILSL
LGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFENNPEQAGNVSQNGERVFIELAKLL
FNPWIAGILLSAILAAVMSTLSCQLLVCSSALTEDLYKAFLRKKASQKELVWVGRAMVLLVALIAIALAADPDNRVLGLV
SYAWAGFGAAFGPVVLISVLWPRMTRNGALVGMLVGAITVLVWKQYGWLDLYEIIPGFLFASLAIFVVSLMGREPSNAIT
ERFHKAEAEFKTV

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=492, Percent_Identity=46.3414634146341, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI167466278, Length=423, Percent_Identity=25.0591016548463, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4507035, Length=383, Percent_Identity=21.9321148825065, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1787251, Length=493, Percent_Identity=80.1217038539554, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1790503, Length=450, Percent_Identity=26.6666666666667, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082237, Length=461, Percent_Identity=25.8134490238612, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI115533094, Length=430, Percent_Identity=24.8837209302326, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17539284, Length=424, Percent_Identity=23.5849056603774, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI115533096, Length=294, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI221459584, Length=380, Percent_Identity=22.3684210526316, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24645928, Length=426, Percent_Identity=25.1173708920188, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24651741, Length=461, Percent_Identity=22.5596529284165, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221459588, Length=429, Percent_Identity=23.3100233100233, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24640370, Length=416, Percent_Identity=24.5192307692308, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53569; Mature: 53438

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNTPMLVTFLVYILGMILIGLIAYRATNNFDDYILGGRRLGSVVTALSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCEE
LMGLPGAVFLSGISESWIAIGLTIGAYLNWKWVAGRLRVHTEANNNALTLPDYFTSRFED
EECCCHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEEEEEEECCCCEEECCHHHHHHHCC
KSKLLRVISAVVILVFFTIYCASGIVAGARLFESTFGMDYGTALWAGAAATIIYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQATLMIFALILTPIIVILAVGGIDTSMMVIAAKNPANIDMFKGLNLVAILS
HEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCCHHHHHHH
LLGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFENNP
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EQAGNVSQNGERVFIELAKLLFNPWIAGILLSAILAAVMSTLSCQLLVCSSALTEDLYKA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKKASQKELVWVGRAMVLLVALIAIALAADPDNRVLGLVSYAWAGFGAAFGPVVLISV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCHHHHHHHHHHH
LWPRMTRNGALVGMLVGAITVLVWKQYGWLDLYEIIPGFLFASLAIFVVSLMGREPSNAI
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
TERFHKAEAEFKTV
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TMNTPMLVTFLVYILGMILIGLIAYRATNNFDDYILGGRRLGSVVTALSAGASDMSGWL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCEE
LMGLPGAVFLSGISESWIAIGLTIGAYLNWKWVAGRLRVHTEANNNALTLPDYFTSRFED
EECCCHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEEEEEEECCCCEEECCHHHHHHHCC
KSKLLRVISAVVILVFFTIYCASGIVAGARLFESTFGMDYGTALWAGAAATIIYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQATLMIFALILTPIIVILAVGGIDTSMMVIAAKNPANIDMFKGLNLVAILS
HEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCCHHHHHHH
LLGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFENNP
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EQAGNVSQNGERVFIELAKLLFNPWIAGILLSAILAAVMSTLSCQLLVCSSALTEDLYKA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKKASQKELVWVGRAMVLLVALIAIALAADPDNRVLGLVSYAWAGFGAAFGPVVLISV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCHHHHHHHHHHH
LWPRMTRNGALVGMLVGAITVLVWKQYGWLDLYEIIPGFLFASLAIFVVSLMGREPSNAI
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
TERFHKAEAEFKTV
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]