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The map label for this gene is hisJ [H]

Identifier: 108812335

GI number: 108812335

Start: 2456284

End: 2457066

Strand: Reverse

Name: hisJ [H]

Synonym: YPN_2173

Alternate gene names: 108812335

Gene position: 2457066-2456284 (Counterclockwise)

Preceding gene: 108812336

Following gene: 108812334

Centisome position: 54.18

GC content: 44.06

Gene sequence:

>783_bases
ATGAATAAAAAGCTGCGAGTTCTTCCTTTGGTATTCGCATTCGCTGCGGCTAGTAGTGCTTTTGCCGCCATTCCAAAAGA
GATCAAAATTGGCACAGATCCGACTTACGCCCCTTTTGAATCGAAAAACTCGAGTGGTGAATTAGTGGGTTTTGATATCG
ATATCGCGAAGGAGTTATGCAAACGTATAGATACCAAATGTACTTTTGTTGAAAGTGACTTTGATGCGCTAATCCCGTCG
CTGAAAGCGAAAAAAGTCGATGCCATTATCTCCTCTCTCTCCATTACTGAAAAACGCCAAAAAGAGATTGCCTTCACCGA
TAAACTCTTTGCCGCCAATTCCCGTTTAATTGCTGCTAAAGGTAGCACTATTGAGCCGACACTGGTGGCATTAAAAGGTA
AGCGCGTGGGGGTGTTACAAGGGTCGACTCAGGAAGCTTTTGCCAATGCTGAGTGGCAGCCAAAAGGCATTGATGTTGTT
GCTTATCAAAATCAGGATCTGATTTATGCAGATCTGGCCTCTGGCCGTATTGATGCGGCTTTCCAGGATGAAGTGGCCGC
CAGTGAAGGTTTCCTAAAACAACCTGTGGGTAAAGATTATATCTTTGCCGGTCCATCAGTAAAAAATGATCAATTCTTCG
GTGTAGGGACTGGCATGGGGTTGCGCAAAGCCGATGTTGAGCTGAAAGAGGCGTTAAATAAAGCGTTTGCTGAAATGCGT
AATGACGGCACCTACGATCAATTTGCCAAAAAATACTTCGATTTTGATGTTTACGGTGATTAA

Upstream 100 bases:

>100_bases
TTACTGGCACGATTACTGCAAATTGATTTATGGACGCTCAGGCTTGGACAGAAATCTAACGCGCTTTGTGGCATCAAAAA
TCAATTTGAGGGTTATGTTT

Downstream 100 bases:

>100_bases
TCCTTTTTGTCGTCGTGGCCTCTGTCGATTGTGGCAGGGGCTAACATACCGTAATTAACCAGACTCCTCACTCAGTAAAA
CAGGATAACCCACAGATCAC

Product: histidine-binding periplasmic protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTFVESDFDALIPS
LKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGIDVV
AYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAEMR
NDGTYDQFAKKYFDFDVYGD

Sequences:

>Translated_260_residues
MNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTFVESDFDALIPS
LKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGIDVV
AYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAEMR
NDGTYDQFAKKYFDFDVYGD
>Mature_260_residues
MNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTFVESDFDALIPS
LKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGIDVV
AYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAEMR
NDGTYDQFAKKYFDFDVYGD

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=78.8135593220339, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=68.8461538461538, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=39.4308943089431, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=34.6938775510204, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=32.1739130434783, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787031, Length=250, Percent_Identity=28.4, Blast_Score=100, Evalue=9e-23,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28355; Mature: 28355

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELC
CCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
KRIDTKCTFVESDFDALIPSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAK
HHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGIDVVAYQNQDLIYADLASGRIDAA
CCCCCCEEEEECCCEEEEEECCCHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCH
FQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAEMR
HHHHHHHCCCHHHCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHC
NDGTYDQFAKKYFDFDVYGD
CCCCHHHHHHHHCCCEECCC
>Mature Secondary Structure
MNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELC
CCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
KRIDTKCTFVESDFDALIPSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAK
HHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGIDVVAYQNQDLIYADLASGRIDAA
CCCCCCEEEEECCCEEEEEECCCHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCH
FQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAEMR
HHHHHHHCCCHHHCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHC
NDGTYDQFAKKYFDFDVYGD
CCCCHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]