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The map label for this gene is nuoB [H]

Identifier: 108812311

GI number: 108812311

Start: 2430878

End: 2431555

Strand: Reverse

Name: nuoB [H]

Synonym: YPN_2149

Alternate gene names: 108812311

Gene position: 2431555-2430878 (Counterclockwise)

Preceding gene: 108812312

Following gene: 108812310

Centisome position: 53.62

GC content: 50.29

Gene sequence:

>678_bases
ATGGACTATACGCTCACCCGCATAGACCCTAACGGTGAGAATGACCGTTACCCCCTGCAAACTCAGGAAACCGTCAGCGG
CGATCCTCTCGAGCAACATGTCCACCGTAGTGTTTACATGGGTAAACTTGAAAACGCCATGCATGACATGGTGAACTGGG
GGCGTAAAAACTCTCTTTGGCCTTATAACTTCGGCCTTTCTTGTTGTTATGTCGAGATGGTGACTTCGTTCACGGCGGTA
CATGACGTCGCCCGTTTCGGTGCTGAAGTCTTGCGTGCATCCCCACGTCAGGCCGATTTTATGGTGGTGGCCGGAACCTG
CTTTACCAAAATGGCCCCGGTTATTCAGCGTTTATATGAACAAATGCTGGAACCAAAATGGGTTATTTCCATGGGGGCCT
GCGCGAATTCTGGTGGCATGTATGATATCTACTCCGTTGTGCAAGGTGTGGATAAATTCCTACCAGTAGATGTCTATATT
CCTGGCTGTCCACCGCGCCCGGAAGCGTATATGCAAGCGCTATTGCTATTGCAGGAATCCATTGGTAAAGAGCGCCGTCC
TCTCTCTTGGGTTGTCGGTGACCAAGGTGTCTACCGTGCCAATATGCAGCCAGAAAGAGAACGTAAGCATGCTGAACGAA
TTGCGGTAACTAACCTGCGCACACCTGACGAGATTTAA

Upstream 100 bases:

>100_bases
CTGATATCAGTCAGTGATTCGGGTCAGTGAACCTAGCTAACAGCCCAGCAGCGTCAAGTGCAAAAGGTATAGTGGTCAAT
AAATATAGCGAGGCATTAAC

Downstream 100 bases:

>100_bases
ACCCGTTTTTGGGGGGAGATCCCGGTGCGTAAGTGATGTGTTTTTGGAGAGAATGTAATTTTTTTCCGGGGCATGTATGC
AGCAAATGACCACAAAATGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLWPYNFGLSCCYVEMVTSFTAV
HDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYI
PGCPPRPEAYMQALLLLQESIGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI

Sequences:

>Translated_225_residues
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLWPYNFGLSCCYVEMVTSFTAV
HDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYI
PGCPPRPEAYMQALLLLQESIGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI
>Mature_225_residues
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLWPYNFGLSCCYVEMVTSFTAV
HDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYI
PGCPPRPEAYMQALLLLQESIGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=145, Percent_Identity=54.4827586206897, Blast_Score=171, Evalue=7e-43,
Organism=Escherichia coli, GI1788624, Length=225, Percent_Identity=90.2222222222222, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=41.8032786885246, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1788834, Length=160, Percent_Identity=35.625, Blast_Score=94, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=145, Percent_Identity=50.3448275862069, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=52.4137931034483, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=52.4137931034483, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24651058, Length=145, Percent_Identity=53.7931034482759, Blast_Score=167, Evalue=5e-42,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 25612; Mature: 25612

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLW
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHH
QMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIPGCPPRPEAYMQALLLLQES
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHH
IGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI
HCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLW
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHH
QMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIPGCPPRPEAYMQALLLLQES
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHH
IGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI
HCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA