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The map label for this gene is nuoF [H]

Identifier: 108812308

GI number: 108812308

Start: 2426993

End: 2428378

Strand: Reverse

Name: nuoF [H]

Synonym: YPN_2146

Alternate gene names: 108812308

Gene position: 2428378-2426993 (Counterclockwise)

Preceding gene: 108812309

Following gene: 108812307

Centisome position: 53.55

GC content: 53.9

Gene sequence:

>1386_bases
ATGACCATCAATTCAGGAACGACCAAGGAAATTATCCGGACGCCAGAAATGCACCCGCTGACCTGGCGTTTGCGCGATGA
CAAACAGCCCGTGTGGCTGGATGAGTATCGCAGCAAAAACGGCTATGCCGGGGCAGAAAAAGCGTTAAAAGGCATGGCGC
CCACTGATGTTGTTAACGTTGTCAAAGACGCTGGCCTGAAAGGGCGTGGCGGCGCAGGTTTCTCCACTGGTTTGAAGTGG
AGCCTGATGCCCAAAGACGACAGTATGAACATCCGCTATCTGCTGTGTAACGCTGATGAGATGGAGCCAGGCACCTATAA
AGACCGTCTGCTGATGGAGCAATTGCCTCATCTGCTGGTGGAGGGGATGCTGATCAGTGCCTTCGCGCTGAAAGCCTACC
GTGGCTATATCTTCCTGCGTGGCGAATATATTGAAGCGGCAGTGCATTTACGTCGGGCGATTGCTGAGGCCACCGAAGCC
GGCTTGCTGGGCAAAAACATCATGGGCAGTGGTTTTGATTTCGAACTGATTGTCCATACCGGTGCGGGTCGTTATATCTG
TGGTGAAGAAACGGCGCTGATTAACTCACTGGAAGGCCGCCGTGCTAACCCGCGTTCTAAGCCGCCGTTCCCGGCCTCTT
CCGGTGTCTGGGGCAAACCGACTTGCGTGAATAACGTTGAAACCCTGTGTAACGTACCGGCCATTATTGAACATGGCGTT
GAGTGGTATCAGGGGATCACTGCCGGTAAGAGTAACGATGCCGGAACCAAACTGATGGGCTTCTCGGGTCGGGTGAAAAA
TCCGGGTCTGTGGGAATTGCCGTTTGGGATCACCGCCCGTGAGATTCTGGAAGATTACGCTGGCGGTATGCGCGATGGCT
TGAAATTTAAAGCCTGGCAGCCGGGCGGGGCGGGTACCGATTTCCTGACTGCTGACCATCTGGATTTGCCGATGGATTTT
GAGAATATTGCTAAAGCAGGTAGCCGGTTAGGCACCGCGTTGGCGATGGCGGTAGATCACGAAATCGGCATGGTGTCTTT
GGTGCGCAATCTGGAAGAGTTCTTCTCCCGTGAGTCTTGTGGCTGGTGTACACCGTGTCGCGATGGTTTGCCATGGAGTG
TGAAGATCTTACGTGCATTAGAGCGTGGTGAAGGCCAGCCGGGTGATATCGAAACGCTAGAGCAACTGTGCCGCTTCTTA
GGGCCGGGTAAAACCTTCTGTGCTCATGCGCCAGGTGCGGTTGAACCCCTACAAAGTGCGATTAAATATTTCCGGGACGA
ATTCGAAGCCGGGATCGCCGTTAAGGCCTATGAATCTGGAATGACGAGCGGCAACACCCGAGCCATAGCCGGTATTCAAC
CTAATCTATTAAAAGCGCGCTGGTAG

Upstream 100 bases:

>100_bases
GTCTGGGTAACTGCGACCGTGGGCCGACGATGATGATCGATGACGACACCCACAGCTACCTGAAGCCTGAAGAGATCGAG
AAGTTACTGGAGCAATATCC

Downstream 100 bases:

>100_bases
CATAGATACCCTTACGGTGTTGCCGTAGGGGTCAGAATTTTTGATTAACGCCTGCCGTAAGGTGGGTCATTTGGAAGCAT
GCTGATATGGCTACGATTCA

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTINSGTTKEIIRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKALKGMAPTDVVNVVKDAGLKGRGGAGFSTGLKW
SLMPKDDSMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEA
GLLGKNIMGSGFDFELIVHTGAGRYICGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAIIEHGV
EWYQGITAGKSNDAGTKLMGFSGRVKNPGLWELPFGITAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTADHLDLPMDF
ENIAKAGSRLGTALAMAVDHEIGMVSLVRNLEEFFSRESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFL
GPGKTFCAHAPGAVEPLQSAIKYFRDEFEAGIAVKAYESGMTSGNTRAIAGIQPNLLKARW

Sequences:

>Translated_461_residues
MTINSGTTKEIIRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKALKGMAPTDVVNVVKDAGLKGRGGAGFSTGLKW
SLMPKDDSMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEA
GLLGKNIMGSGFDFELIVHTGAGRYICGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAIIEHGV
EWYQGITAGKSNDAGTKLMGFSGRVKNPGLWELPFGITAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTADHLDLPMDF
ENIAKAGSRLGTALAMAVDHEIGMVSLVRNLEEFFSRESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFL
GPGKTFCAHAPGAVEPLQSAIKYFRDEFEAGIAVKAYESGMTSGNTRAIAGIQPNLLKARW
>Mature_460_residues
TINSGTTKEIIRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKALKGMAPTDVVNVVKDAGLKGRGGAGFSTGLKWS
LMPKDDSMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEAG
LLGKNIMGSGFDFELIVHTGAGRYICGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAIIEHGVE
WYQGITAGKSNDAGTKLMGFSGRVKNPGLWELPFGITAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTADHLDLPMDFE
NIAKAGSRLGTALAMAVDHEIGMVSLVRNLEEFFSRESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
PGKTFCAHAPGAVEPLQSAIKYFRDEFEAGIAVKAYESGMTSGNTRAIAGIQPNLLKARW

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=390, Percent_Identity=44.1025641025641, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI20149568, Length=390, Percent_Identity=44.1025641025641, Blast_Score=317, Evalue=1e-86,
Organism=Escherichia coli, GI1788620, Length=453, Percent_Identity=88.962472406181, Blast_Score=827, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17531727, Length=379, Percent_Identity=41.688654353562, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI19920794, Length=397, Percent_Identity=43.3249370277078, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI45550452, Length=384, Percent_Identity=44.0104166666667, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24653819, Length=379, Percent_Identity=34.0369393139842, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24653817, Length=354, Percent_Identity=32.4858757062147, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50453; Mature: 50322

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINSGTTKEIIRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKALKGMAPTDVVNV
CCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCHHHHHCCCCHHHHHHH
VKDAGLKGRGGAGFSTGLKWSLMPKDDSMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLV
HHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
EGMLISAFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEAGLLGKNIMGSGFDFELIVHT
HHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
GAGRYICGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAIIEHGV
CCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
EWYQGITAGKSNDAGTKLMGFSGRVKNPGLWELPFGITAREILEDYAGGMRDGLKFKAWQ
HHHHCCCCCCCCCCCCEEECCCCCCCCCCCEECCCCCCHHHHHHHHCCCHHCCCEEEEEC
PGGAGTDFLTADHLDLPMDFENIAKAGSRLGTALAMAVDHEIGMVSLVRNLEEFFSRESC
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
GWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAHAPGAVEPLQSA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHH
IKYFRDEFEAGIAVKAYESGMTSGNTRAIAGIQPNLLKARW
HHHHHHHHHCCEEEEEHHCCCCCCCCEEEECCCCCHHCCCC
>Mature Secondary Structure 
TINSGTTKEIIRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKALKGMAPTDVVNV
CCCCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCHHHHHCCCCHHHHHHH
VKDAGLKGRGGAGFSTGLKWSLMPKDDSMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLV
HHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
EGMLISAFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEAGLLGKNIMGSGFDFELIVHT
HHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
GAGRYICGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAIIEHGV
CCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
EWYQGITAGKSNDAGTKLMGFSGRVKNPGLWELPFGITAREILEDYAGGMRDGLKFKAWQ
HHHHCCCCCCCCCCCCEEECCCCCCCCCCCEECCCCCCHHHHHHHHCCCHHCCCEEEEEC
PGGAGTDFLTADHLDLPMDFENIAKAGSRLGTALAMAVDHEIGMVSLVRNLEEFFSRESC
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
GWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAHAPGAVEPLQSA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHH
IKYFRDEFEAGIAVKAYESGMTSGNTRAIAGIQPNLLKARW
HHHHHHHHHCCEEEEEHHCCCCCCCCEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]