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The map label for this gene is nuoE [H]

Identifier: 108812309

GI number: 108812309

Start: 2428375

End: 2428938

Strand: Reverse

Name: nuoE [H]

Synonym: YPN_2147

Alternate gene names: 108812309

Gene position: 2428938-2428375 (Counterclockwise)

Preceding gene: 108812310

Following gene: 108812308

Centisome position: 53.56

GC content: 54.08

Gene sequence:

>564_bases
ATGAGTGATCAAAAAGAGACTCAGGCTCATAACGGAAGCCTTGATGTGGTGAATCTGGCAGTCAATGCGGCTGAACCAGC
GGCCATGCCAGACGCCTTCGAATTGAGTGCCGAAGAACGTGATGCTATCGAGCATGAGAAACACCATTACGAAGATGCCC
GTGCTGCGTCGATTGAAGCACTGAAAATTGTGCAGAAGCAACGTGGCTGGGTGCCGGATGGTGCGATCCACGCGATTGCT
GATGTGCTGGGTATCCCGGCCAGTGATGTTGAGGGGGTCGCCACCTTCTACAGCCAAATCTTCCGCCAACCGGTAGGGCG
TCATGTGATCCGCTACTGTGACAGCGTGGTGTGTCATATCACCGGCTATCAGGGGATTCAGGCGGCACTGGAGAAAAAAC
TCAGTATCAAGCCAGGCCAAACGACCTTCGATGGCCGTTTCACCCTGCTGCCAACCTGTTGTCTGGGTAACTGCGACCGT
GGGCCGACGATGATGATCGATGACGACACCCACAGCTACCTGAAGCCTGAAGAGATCGAGAAGTTACTGGAGCAATATCC
ATGA

Upstream 100 bases:

>100_bases
TGCAGCAGATCCCATCGGTTATCCGTGGCAGTTTGGTATCTGACCTGATCGTCTATCTGGGCAGTATCGATTTTGTAATG
TCTGATGTGGACCGCTAATT

Downstream 100 bases:

>100_bases
CCATCAATTCAGGAACGACCAAGGAAATTATCCGGACGCCAGAAATGCACCCGCTGACCTGGCGTTTGCGCGATGACAAA
CAGCCCGTGTGGCTGGATGA

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MSDQKETQAHNGSLDVVNLAVNAAEPAAMPDAFELSAEERDAIEHEKHHYEDARAASIEALKIVQKQRGWVPDGAIHAIA
DVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQGIQAALEKKLSIKPGQTTFDGRFTLLPTCCLGNCDR
GPTMMIDDDTHSYLKPEEIEKLLEQYP

Sequences:

>Translated_187_residues
MSDQKETQAHNGSLDVVNLAVNAAEPAAMPDAFELSAEERDAIEHEKHHYEDARAASIEALKIVQKQRGWVPDGAIHAIA
DVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQGIQAALEKKLSIKPGQTTFDGRFTLLPTCCLGNCDR
GPTMMIDDDTHSYLKPEEIEKLLEQYP
>Mature_186_residues
SDQKETQAHNGSLDVVNLAVNAAEPAAMPDAFELSAEERDAIEHEKHHYEDARAASIEALKIVQKQRGWVPDGAIHAIAD
VLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQGIQAALEKKLSIKPGQTTFDGRFTLLPTCCLGNCDRG
PTMMIDDDTHSYLKPEEIEKLLEQYP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=170, Percent_Identity=35.2941176470588, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1788621, Length=156, Percent_Identity=89.1025641025641, Blast_Score=297, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17561328, Length=154, Percent_Identity=35.7142857142857, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859877, Length=170, Percent_Identity=34.1176470588235, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355353, Length=153, Percent_Identity=30.718954248366, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20648; Mature: 20517

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQKETQAHNGSLDVVNLAVNAAEPAAMPDAFELSAEERDAIEHEKHHYEDARAASIEA
CCCCHHHHHCCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LKIVQKQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHI
HHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TGYQGIQAALEKKLSIKPGQTTFDGRFTLLPTCCLGNCDRGPTMMIDDDTHSYLKPEEIE
CCCHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEECCCCCCCCHHHHH
KLLEQYP
HHHHHCC
>Mature Secondary Structure 
SDQKETQAHNGSLDVVNLAVNAAEPAAMPDAFELSAEERDAIEHEKHHYEDARAASIEA
CCCHHHHHCCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LKIVQKQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHI
HHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TGYQGIQAALEKKLSIKPGQTTFDGRFTLLPTCCLGNCDRGPTMMIDDDTHSYLKPEEIE
CCCHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEECCCCCCCCHHHHH
KLLEQYP
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]