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The map label for this gene is rbsB [H]

Identifier: 108812257

GI number: 108812257

Start: 2372595

End: 2373512

Strand: Reverse

Name: rbsB [H]

Synonym: YPN_2095

Alternate gene names: 108812257

Gene position: 2373512-2372595 (Counterclockwise)

Preceding gene: 108812258

Following gene: 108812256

Centisome position: 52.34

GC content: 46.3

Gene sequence:

>918_bases
ATGTTTTCATTTTTCAAAAAAGCCCTGTCATTTAGCATTGCAGGCGTCATGCTGTTTGCCAGCCAAAGTGTCATGGCTAA
GCAAATTGTTATTGGTGTTTCATTTCAAGAATTAAATAACGACTATTTTGTTTCGATGAAAGAGGCATTAGAACAGGCGG
CCAATGATATTGGTGCCAAAGTATATATCGCTGATGCGGGTCATGATGTCTCAAAACAGATTAATGACGTCGAAGACATG
TTGCAAAAGAAAATCGATATTCTGCTTATTAACCCAACGGATTCCGTTGGCGTTCAATCGGCGGTTATGGCGGCGAATAA
AGCGGGTGCGGTTGTGGTGGCCGTTGATGCACAAGCAGAAGGTCCGCTGGACTCTTTCGTGGGTTCAAAGAACTATGATG
CAGGCTTTTTAGCCGGTGAGTATCTGGCGAAATCACTGGGGGGGAAAGGGAAGGTCGCGATCCTGGATGGCATCCCCGTA
GTGCCAATTCTTGAGCGTGTACGGGGTTTTGAAGATGCGATAAAAAAATATCCAGACATCAAAATTGTCACCAAACAAAA
CGGCAAGCAGAAGCGTGATACGGCCTTGACGGTAACCGAAAATATGTTGCAGTCGGTCCCTGATCTGGCCGGGATTTTCA
GTGTGAATGATGTTGGTGCATTGGGTGCACTGGCAGCAATCGAAAGTAATGGCGCAAAAGTAAAGCTGGTTAGCGTAGAC
GGCCAACCAGAAGCCATTAAAGAGATCCTGAAACCCAATTCACCTTTTATTGCGACTTCCGCACAGTTTCCTCGTGACCA
AATACGTATTGCACTGGGTATCGCGTTGGCCCGTTATTGGGGAGCCAATGTTCCGGCAACTATTCCGGTCGAAGTGAAAC
TGATCGACAGTAGCAATGCGCAAGGTTTTAGCTGGTAA

Upstream 100 bases:

>100_bases
TATATCATGTGGGATATTAACTTTTTATGATGAAAGTGAAGTAAAAACATACCGTTGTTCTGATAACCAAAAATACACTT
CCCCTACAGGAGAATAATAT

Downstream 100 bases:

>100_bases
GCGTGTCAATTCTCGCTCTGCTCAGGCAGAGCGATAACCATGACGCCTTTATTAGAGTTAAAGCAGATAAAAAAATCTTT
TCCGGGTGTTAAAGCACTGG

Product: sugar binding protein precursor

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MFSFFKKALSFSIAGVMLFASQSVMAKQIVIGVSFQELNNDYFVSMKEALEQAANDIGAKVYIADAGHDVSKQINDVEDM
LQKKIDILLINPTDSVGVQSAVMAANKAGAVVVAVDAQAEGPLDSFVGSKNYDAGFLAGEYLAKSLGGKGKVAILDGIPV
VPILERVRGFEDAIKKYPDIKIVTKQNGKQKRDTALTVTENMLQSVPDLAGIFSVNDVGALGALAAIESNGAKVKLVSVD
GQPEAIKEILKPNSPFIATSAQFPRDQIRIALGIALARYWGANVPATIPVEVKLIDSSNAQGFSW

Sequences:

>Translated_305_residues
MFSFFKKALSFSIAGVMLFASQSVMAKQIVIGVSFQELNNDYFVSMKEALEQAANDIGAKVYIADAGHDVSKQINDVEDM
LQKKIDILLINPTDSVGVQSAVMAANKAGAVVVAVDAQAEGPLDSFVGSKNYDAGFLAGEYLAKSLGGKGKVAILDGIPV
VPILERVRGFEDAIKKYPDIKIVTKQNGKQKRDTALTVTENMLQSVPDLAGIFSVNDVGALGALAAIESNGAKVKLVSVD
GQPEAIKEILKPNSPFIATSAQFPRDQIRIALGIALARYWGANVPATIPVEVKLIDSSNAQGFSW
>Mature_305_residues
MFSFFKKALSFSIAGVMLFASQSVMAKQIVIGVSFQELNNDYFVSMKEALEQAANDIGAKVYIADAGHDVSKQINDVEDM
LQKKIDILLINPTDSVGVQSAVMAANKAGAVVVAVDAQAEGPLDSFVGSKNYDAGFLAGEYLAKSLGGKGKVAILDGIPV
VPILERVRGFEDAIKKYPDIKIVTKQNGKQKRDTALTVTENMLQSVPDLAGIFSVNDVGALGALAAIESNGAKVKLVSVD
GQPEAIKEILKPNSPFIATSAQFPRDQIRIALGIALARYWGANVPATIPVEVKLIDSSNAQGFSW

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=296, Percent_Identity=35.472972972973, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1790526, Length=308, Percent_Identity=30.1948051948052, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32434; Mature: 32434

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSFFKKALSFSIAGVMLFASQSVMAKQIVIGVSFQELNNDYFVSMKEALEQAANDIGAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCEEEEHHHHHHHHHHHCCCE
VYIADAGHDVSKQINDVEDMLQKKIDILLINPTDSVGVQSAVMAANKAGAVVVAVDAQAE
EEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCCEEEEEEECCCC
GPLDSFVGSKNYDAGFLAGEYLAKSLGGKGKVAILDGIPVVPILERVRGFEDAIKKYPDI
CCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHHHHHCCCE
KIVTKQNGKQKRDTALTVTENMLQSVPDLAGIFSVNDVGALGALAAIESNGAKVKLVSVD
EEEECCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCCCHHHHHHHHEECCCCEEEEEEEC
GQPEAIKEILKPNSPFIATSAQFPRDQIRIALGIALARYWGANVPATIPVEVKLIDSSNA
CCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
QGFSW
CCCCC
>Mature Secondary Structure
MFSFFKKALSFSIAGVMLFASQSVMAKQIVIGVSFQELNNDYFVSMKEALEQAANDIGAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCEEEEHHHHHHHHHHHCCCE
VYIADAGHDVSKQINDVEDMLQKKIDILLINPTDSVGVQSAVMAANKAGAVVVAVDAQAE
EEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCCEEEEEEECCCC
GPLDSFVGSKNYDAGFLAGEYLAKSLGGKGKVAILDGIPVVPILERVRGFEDAIKKYPDI
CCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHHHHHCCCE
KIVTKQNGKQKRDTALTVTENMLQSVPDLAGIFSVNDVGALGALAAIESNGAKVKLVSVD
EEEECCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCCCHHHHHHHHEECCCCEEEEEEEC
GQPEAIKEILKPNSPFIATSAQFPRDQIRIALGIALARYWGANVPATIPVEVKLIDSSNA
CCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
QGFSW
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]