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The map label for this gene is gutB [H]

Identifier: 108812258

GI number: 108812258

Start: 2373687

End: 2374913

Strand: Reverse

Name: gutB [H]

Synonym: YPN_2096

Alternate gene names: 108812258

Gene position: 2374913-2373687 (Counterclockwise)

Preceding gene: 108812259

Following gene: 108812257

Centisome position: 52.37

GC content: 47.11

Gene sequence:

>1227_bases
ATGATACTCAATGTTTACGTTGTCATTTCAATATTTATTATGAATGCGATGTCATTGTTGAGCGTTGAAGTGAGTAATTT
TACTTTTGTTGGTTTGTTCAAAAATGACAACGTCAACATTGTATCCATACACCGAGTTCTGTTGCTATTGGAGGAGTTAA
TGAGTCAGTTACCTGAGAAAATGAGCGCTGTCGTATGTCACGGCCCGCAGGATTATCGTTTAGAACACGTGCCGACACCT
GTGCCGAAAGCCAAAGAGTTGGTGATCAAAGTGAAAGGCTGTGGGATTTGTGCAGGTGACTGTAAATGTAAGAATGGGGC
AAAAATGTTTTGGGGAGAAACCCCGTGGGTAAAACCACCAGTGGTACCGGGACATGAATTCTACGGCCATATTGTGGCGC
TGGGGGAAGGGACCGAATCAAAGTATAAGTTAGGCGAACGTGTGATTGCGGAACAAATAGTCCCTTGCTGGGACTGTCGC
TATTGCAACTCTGGCAGCTACTGGATGTGTGAAACGCACAATATCTATGGCTTCCAAAAAGATGTCGCAGAAGGTGGCAT
GGCGGAATATATGCGTTTTTCTGAGAATGCCATTGTTCACAAAATCCCAGAGAGCCTGAGTCATGAAGATGCGGTCCTGA
TCGAACCCATGGCCTGTGCGATTCATACTGTGGCAAGAGGGGATATTCAACTGGATGATGTGGTCGTACTGGCGGGCGCG
GGGCCGCTGGGGCTGTGCATGGTACCGGTTGCCCGCCTGAAGACACCGAAGAAATTGATTGTCATTGATACTATTGAAGA
ACGCCTGGCACTGGCAAAAGCCTTTGGGGCCGATGTGGTGATCAACCCACTGAAAGAAGATGCTGACAAAATAGTGAAAT
CTTTGACCGGTGGCTATGGCTGCGACGTTTACATTGAAGCGACGGGTGCGCCCATCGGTGTGACTCAAGGTTTACAAATG
ATCCGCAAGCTGGGGCGTTTTGTGGAGTTCAGTGTCTTTGGTCAGGAAACTACCGTTGATTGGTCCATTATCGGCGACCG
TAAAGAACTGGATATTCGCGGTGCTCACCTGGCACCTTACAGTTATGAAATTGCCACCGATCTCTTTGAACGTGGTCTGG
TTACCTCAAAAGGTATCGTCACCCACAGTTATTCATTAGATGATTGGGAAAAGGCTTTTGAGCTAGCGGACTCCACGAAT
TCTATTAAGGTCATTCTGGTTCCTTAA

Upstream 100 bases:

>100_bases
GACGGTTGTTGCGATCAGGATCTAAATTAATGACTTTGTTTAACATCAATTTAACAGCGTGTGACTTTGTTCAACAAATA
GTTTCTGCCAATTTGGTTAT

Downstream 100 bases:

>100_bases
TTATTGTTAGTAATTATCTGCTGATGTTTGATATTGACGGTTTATTTATCTTACTCAAAGGTAAAGTACCGTTTTATATC
ATGTGGGATATTAACTTTTT

Product: zinc-binding dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MILNVYVVISIFIMNAMSLLSVEVSNFTFVGLFKNDNVNIVSIHRVLLLLEELMSQLPEKMSAVVCHGPQDYRLEHVPTP
VPKAKELVIKVKGCGICAGDCKCKNGAKMFWGETPWVKPPVVPGHEFYGHIVALGEGTESKYKLGERVIAEQIVPCWDCR
YCNSGSYWMCETHNIYGFQKDVAEGGMAEYMRFSENAIVHKIPESLSHEDAVLIEPMACAIHTVARGDIQLDDVVVLAGA
GPLGLCMVPVARLKTPKKLIVIDTIEERLALAKAFGADVVINPLKEDADKIVKSLTGGYGCDVYIEATGAPIGVTQGLQM
IRKLGRFVEFSVFGQETTVDWSIIGDRKELDIRGAHLAPYSYEIATDLFERGLVTSKGIVTHSYSLDDWEKAFELADSTN
SIKVILVP

Sequences:

>Translated_408_residues
MILNVYVVISIFIMNAMSLLSVEVSNFTFVGLFKNDNVNIVSIHRVLLLLEELMSQLPEKMSAVVCHGPQDYRLEHVPTP
VPKAKELVIKVKGCGICAGDCKCKNGAKMFWGETPWVKPPVVPGHEFYGHIVALGEGTESKYKLGERVIAEQIVPCWDCR
YCNSGSYWMCETHNIYGFQKDVAEGGMAEYMRFSENAIVHKIPESLSHEDAVLIEPMACAIHTVARGDIQLDDVVVLAGA
GPLGLCMVPVARLKTPKKLIVIDTIEERLALAKAFGADVVINPLKEDADKIVKSLTGGYGCDVYIEATGAPIGVTQGLQM
IRKLGRFVEFSVFGQETTVDWSIIGDRKELDIRGAHLAPYSYEIATDLFERGLVTSKGIVTHSYSLDDWEKAFELADSTN
SIKVILVP
>Mature_408_residues
MILNVYVVISIFIMNAMSLLSVEVSNFTFVGLFKNDNVNIVSIHRVLLLLEELMSQLPEKMSAVVCHGPQDYRLEHVPTP
VPKAKELVIKVKGCGICAGDCKCKNGAKMFWGETPWVKPPVVPGHEFYGHIVALGEGTESKYKLGERVIAEQIVPCWDCR
YCNSGSYWMCETHNIYGFQKDVAEGGMAEYMRFSENAIVHKIPESLSHEDAVLIEPMACAIHTVARGDIQLDDVVVLAGA
GPLGLCMVPVARLKTPKKLIVIDTIEERLALAKAFGADVVINPLKEDADKIVKSLTGGYGCDVYIEATGAPIGVTQGLQM
IRKLGRFVEFSVFGQETTVDWSIIGDRKELDIRGAHLAPYSYEIATDLFERGLVTSKGIVTHSYSLDDWEKAFELADSTN
SIKVILVP

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=355, Percent_Identity=30.1408450704225, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI71565154, Length=383, Percent_Identity=25.3263707571802, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4501929, Length=381, Percent_Identity=24.1469816272966, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1787863, Length=351, Percent_Identity=31.9088319088319, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1788073, Length=353, Percent_Identity=28.328611898017, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1790045, Length=337, Percent_Identity=30.8605341246291, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788075, Length=361, Percent_Identity=28.2548476454294, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87082125, Length=359, Percent_Identity=29.5264623955432, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI226510992, Length=347, Percent_Identity=27.3775216138329, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788407, Length=345, Percent_Identity=26.0869565217391, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786825, Length=404, Percent_Identity=25.7425742574257, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790718, Length=324, Percent_Identity=25.6172839506173, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081918, Length=222, Percent_Identity=33.7837837837838, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1786552, Length=362, Percent_Identity=30.1104972375691, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562876, Length=361, Percent_Identity=30.4709141274238, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17562878, Length=357, Percent_Identity=28.8515406162465, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI25146526, Length=389, Percent_Identity=23.9074550128535, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71997431, Length=389, Percent_Identity=23.6503856041131, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323099, Length=352, Percent_Identity=28.4090909090909, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319955, Length=364, Percent_Identity=28.021978021978, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322619, Length=364, Percent_Identity=27.7472527472527, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319258, Length=373, Percent_Identity=26.0053619302949, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6319257, Length=300, Percent_Identity=25.6666666666667, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=334, Percent_Identity=26.6467065868263, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323980, Length=195, Percent_Identity=29.7435897435897, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323729, Length=347, Percent_Identity=25.3602305475504, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320033, Length=278, Percent_Identity=26.2589928057554, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323961, Length=333, Percent_Identity=26.1261261261261, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=253, Percent_Identity=28.4584980237154, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17137530, Length=348, Percent_Identity=27.5862068965517, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17737897, Length=347, Percent_Identity=27.9538904899135, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17737895, Length=385, Percent_Identity=24.6753246753247, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 44954; Mature: 44954

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNVYVVISIFIMNAMSLLSVEVSNFTFVGLFKNDNVNIVSIHRVLLLLEELMSQLPEK
CEEEHHHHHHHHHHHHHHHHEEEECCEEEEEEEECCCEEEEHHHHHHHHHHHHHHHCHHH
MSAVVCHGPQDYRLEHVPTPVPKAKELVIKVKGCGICAGDCKCKNGAKMFWGETPWVKPP
HCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCCEEEECCCCCCCCC
VVPGHEFYGHIVALGEGTESKYKLGERVIAEQIVPCWDCRYCNSGSYWMCETHNIYGFQK
CCCCCHHCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCHH
DVAEGGMAEYMRFSENAIVHKIPESLSHEDAVLIEPMACAIHTVARGDIQLDDVVVLAGA
HHHCCCHHHHHHHCCCCHHHHCHHHCCCCCEEEECHHHHHHHHHHCCCEEECCEEEEECC
GPLGLCMVPVARLKTPKKLIVIDTIEERLALAKAFGADVVINPLKEDADKIVKSLTGGYG
CCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCC
CDVYIEATGAPIGVTQGLQMIRKLGRFVEFSVFGQETTVDWSIIGDRKELDIRGAHLAPY
EEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCEEEEEEEECCCCCEEECCCEECCC
SYEIATDLFERGLVTSKGIVTHSYSLDDWEKAFELADSTNSIKVILVP
HHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MILNVYVVISIFIMNAMSLLSVEVSNFTFVGLFKNDNVNIVSIHRVLLLLEELMSQLPEK
CEEEHHHHHHHHHHHHHHHHEEEECCEEEEEEEECCCEEEEHHHHHHHHHHHHHHHCHHH
MSAVVCHGPQDYRLEHVPTPVPKAKELVIKVKGCGICAGDCKCKNGAKMFWGETPWVKPP
HCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCCEEEECCCCCCCCC
VVPGHEFYGHIVALGEGTESKYKLGERVIAEQIVPCWDCRYCNSGSYWMCETHNIYGFQK
CCCCCHHCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCHH
DVAEGGMAEYMRFSENAIVHKIPESLSHEDAVLIEPMACAIHTVARGDIQLDDVVVLAGA
HHHCCCHHHHHHHCCCCHHHHCHHHCCCCCEEEECHHHHHHHHHHCCCEEECCEEEEECC
GPLGLCMVPVARLKTPKKLIVIDTIEERLALAKAFGADVVINPLKEDADKIVKSLTGGYG
CCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCC
CDVYIEATGAPIGVTQGLQMIRKLGRFVEFSVFGQETTVDWSIIGDRKELDIRGAHLAPY
EEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCEEEEEEEECCCCCEEECCCEECCC
SYEIATDLFERGLVTSKGIVTHSYSLDDWEKAFELADSTNSIKVILVP
HHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]