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The map label for this gene is mltF [H]

Identifier: 108811380

GI number: 108811380

Start: 1379871

End: 1381412

Strand: Reverse

Name: mltF [H]

Synonym: YPN_1217

Alternate gene names: 108811380

Gene position: 1381412-1379871 (Counterclockwise)

Preceding gene: 108811382

Following gene: 108811376

Centisome position: 30.46

GC content: 49.09

Gene sequence:

>1542_bases
ATGCCACTCCACTGGGGATTTAACCATGTTGAATTTAGCTTTACCGCACACACGAAGAGAGCGTTTCCGAGAGATAACTA
TTTGACACGCATAAAATTAAGTTACTTTACCATCGGTCTGGTTGCTCTGCTTCTGGCGCTTGCGCTGTGGCCCAATATTC
CTTGGCGTAATGGGCAAGAGGGCCAACTTGACCAAATAAAAGCCCGTGGAGAACTGCGCGTCAGTACCATCAGTTCTCCT
TTGATCTACTCTACCGAAAAGGATACCCCTTCCGGTTTTGACTATGAGCTAGCAAAACGTTTTGCTGACTATCTGGGGGT
TAAACTGGTTATCATCCCCCATCATAATATTGACGATCTGTTTGACGCATTAGATAACGATGACACCGATCTGCTAGCCG
CTGGGCTGATTTATAACCGTGAACGCCTCAATCGGGCGCGGACTGGCCCTGCCTACTATTCTGTTTCTCAGCAATTGGTC
TACCGGCTGGGTTCGCCACGGCCTAAATCGTTCAGCGATCTGAAAGGTCAGGTGGTTGTGGCCTCCGGTTCGGCCCATAT
GACCACACTAAAACGGCTTAAGCAGACAAAATATCCTGAACTCAATTGGAGTTCATCCGTCGATAAGTCCGGTAAAGAAT
TACTGGAGCAAGTCGCGGAAGGTAAGCTCGACTATACCCTTGGCGATTCAGCCACCATTGCCCTGTTACAACGCATTCAT
CCGCAATTGGCAGTCGCATTTGATGTCACCGATGAAGAGCCCGTCACCTGGTATTTTAAACAGAGTGATGACGATAGCCT
GTATGCGGCCATGCTCGATTTCTACAGTGAGATGGTGGAAGACGGTAGCCTCGCACGTCTGGAGGAGAAATATCTGGGTC
ATGTGGGGAGTTTTGACTATGTTGATACCAAAACTTTCCTGTCCGCTATCGATAATGTCCTGCCCTCTTACCAGCACCTA
TTTGAAAAACATGCAGGGGACATTGACTGGAAATTGCTGGCCGTGATTGCCTATCAAGAATCACATTGGAACCCGCAAGC
CACCTCCCCTACTGGTGTTCGTGGCTTGATGATGCTAACCCGTGCGACCGCGGACGGGTTAGGGGTCAAGGATAGGGTTG
ACCCTGAAGAAAGCATCAGAGGCGGGGCTATCTATTTGCAACGGCTGATGAAAAAACTCCCTGAAACCATCCCAGAAGAT
GAACGCATCTGGTTTGCACTGGCTGCGTATAATTTAGGCTATGGTCATATGCTGGATGCCCGCCGTTTGACTAAAAATCA
GAACGGTAACCCAGACAGTTGGGTTGATGTGAAAATGCGTTTACCGATGCTAAGCCAAAAACGCTACTACCCAAGCACCA
CTTACGGTTATGCCCGTGGTCATGAAGCCTATAACTATGTCGAGAATATTCGCCGTTATCAGGTCAGTCTGGTGGGATAT
TTACAGGAAAAAGAGAAAAAAGCGGCTCAACATGCCGCCATTGAGGCGGAGCTGGGCAAAAGCAACCCGGTTGTCGGCCC
CGGTTGGTCAATCGGTGACTAA

Upstream 100 bases:

>100_bases
ATTATTAATAATTCAGTCAGAAACAGCCGCTATGTGTGTGTAACGCAGACTCAGCAAATAGCTCGCAATAAAGTTGCACA
CTGGCGTTTTGTTGCGCAAA

Downstream 100 bases:

>100_bases
TTGCTCGTCAGTCACTCGTCGGCAGTCGTTCAACCTTAATTACACCGTGATTTAGTCACCAACCGCCGCCCGTCATAACA
TGCTGGGCGGCGCATCACCT

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MPLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVSTISSP
LIYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLV
YRLGSPRPKSFSDLKGQVVVASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALLQRIH
PQLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPSYQHL
FEKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPED
ERIWFALAAYNLGYGHMLDARRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVSLVGY
LQEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD

Sequences:

>Translated_513_residues
MPLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVSTISSP
LIYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLV
YRLGSPRPKSFSDLKGQVVVASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALLQRIH
PQLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPSYQHL
FEKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPED
ERIWFALAAYNLGYGHMLDARRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVSLVGY
LQEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD
>Mature_512_residues
PLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVSTISSPL
IYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLVY
RLGSPRPKSFSDLKGQVVVASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALLQRIHP
QLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPSYQHLF
EKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPEDE
RIWFALAAYNLGYGHMLDARRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVSLVGYL
QEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=481, Percent_Identity=60.7068607068607, Blast_Score=595, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 58079; Mature: 57948

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWRNGQE
CCCCCCCCCEEEEEEEHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GQLDQIKARGELRVSTISSPLIYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHNIDDL
CCHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHCEEEEEECCCCHHHH
FDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLVYRLGSPRPKSFSDLKGQVVV
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
ASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALLQRIH
ECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHC
PQLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDY
CCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
VDTKTFLSAIDNVLPSYQHLFEKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGLMMLT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCCCCCHHHHHHHH
RATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPEDERIWFALAAYNLGYGHMLDA
HHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHH
RRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVSLVGY
HHHHCCCCCCCCCEEEEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LQEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLHWGFNHVEFSFTAHTKRAFPRDNYLTRIKLSYFTIGLVALLLALALWPNIPWRNGQE
CCCCCCCCEEEEEEEHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GQLDQIKARGELRVSTISSPLIYSTEKDTPSGFDYELAKRFADYLGVKLVIIPHHNIDDL
CCHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHCEEEEEECCCCHHHH
FDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLVYRLGSPRPKSFSDLKGQVVV
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
ASGSAHMTTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALLQRIH
ECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHC
PQLAVAFDVTDEEPVTWYFKQSDDDSLYAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDY
CCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
VDTKTFLSAIDNVLPSYQHLFEKHAGDIDWKLLAVIAYQESHWNPQATSPTGVRGLMMLT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCCCCCHHHHHHHH
RATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPEDERIWFALAAYNLGYGHMLDA
HHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHH
RRLTKNQNGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVSLVGY
HHHHCCCCCCCCCEEEEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LQEKEKKAAQHAAIEAELGKSNPVVGPGWSIGD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA