Click here to switch to the map view.

The map label for this gene is nrdE [H]

Identifier: 108811298

GI number: 108811298

Start: 1303182

End: 1305332

Strand: Reverse

Name: nrdE [H]

Synonym: YPN_1135

Alternate gene names: 108811298

Gene position: 1305332-1303182 (Counterclockwise)

Preceding gene: 108811299

Following gene: 108811297

Centisome position: 28.79

GC content: 46.58

Gene sequence:

>2151_bases
TTGGCAACGACAAAACTGAACGTCATCGATTCATCCGACAGTGAGCTGGATTATCACTCACTGAATGCAATGCTGAACCT
TTATGATGCTGATGGGCGTATCCAGTTTGATAAAGACCGGCTGGCCGCACGGCAATACTTTTTGCAGCACGTTAATCAAA
ACACGGTGTTCTTCCATAATCTACAGGAAAAAATTGATTACCTGATTGAGGAGGGATATTACGAAAAAGAGGTTCTGGAT
CAATACGATTTTAGCTTTATCAAAAGTCTATTCCAGCAAGCCTATAGCAAAAAATTTCGTTTCCCCACTTTTCTCGGGGC
GTTTAAATATTACACCAGCTACACCCTAAAAACCTTTGATGGCAAACGTTACCTGGAACGCTATGAAGATCGCGTTTGCA
TGGTCGCACTGACACTGGCTACAGGTAACCAGACACTGGCGCGGGAACTGGTAGAAGAAATTATCTCTGGCCGCTTCCAA
CCCGCCACACCAACCTTCCTCAATTGTGGCAAAAAACAACGTGGTGAACTGGTCTCCTGCTTCCTGTTGCGTATCGAAGA
CAATATGGAATCCATTGGCCGCTCGATTAACTCGGCACTGCAACTCTCTAAACGTGGCGGAGGCGTGGCGTTCCTCCTCA
GCAATATCCGTGAGGTGGGTGCTCCCATCAAACGGATTGAAAATCAGTCTTCGGGTGTTATCCCGATAATGAAAATGTTG
GAGGACGCGTTCTCCTATGCCAATCAGTTGGGTGCCCGTCAGGGGGCGGGGGCGGTCTATCTCCATGCCCATCATCCAGA
TATTTTGCGTTTTCTGGATACCAAGCGGGAAAATGCCGATGAAAAAGTACGGATTAAGACCTTATCGTTGGGGGTGGTGA
TACCGGATATCACTTTTGAACTGGCGAAGAATAATGAAGAAATGTACCTGTTTTCACCTTATGACATTGAACAGGTTTAC
GGCGTGCCCATGTCTGAAATCAGCATCAGCGAAAAATACCGCGAAATGGTGGATAACAAACGCATCCGCAAAAGCAAAAT
TAAAGCCCGCGAGTTCTTCCAAATTCTGGCTGAGATCCAATTTGAATCTGGCTACCCTTATATGATGTTTGAAGATACGG
TTAATCGCGCTAATCCGATTAAAGGCCGGATTAATATGAGTAATTTGTGTTCGGAAATTTTGCAGGTCAACGCCCCCACT
GAATACCACGAAGACCTCAGCTATCGCCGTATCGGGAAAGATATCTCCTGTAATCTTGGCTCGCTGAATATCGCGCAGGT
GATGGATTCACCCGATTTTGGTAAATCCATTGAGATCGCCATTCGTGGGCTCACTGCCGTATCTGAAATGAGCAATATTC
GCTCAGTGCCATCCATTGAAAAAGGCAATCAAGAATCACATGCCATTGGCCTTGGGCAGATGAACCTGCACGGTTATCTG
GCGCGGGAGCGTCTTTTTTACGGCTCGGAAGAGGCGTTGGATTTTACCAATATCTATTTTTATACCGTGGCGTATCACGC
GATCCGTGCCTCAAACCAGTTGGCAATAGAACGGGCCAGCCATTTCAAAGGCTTCGATGAATCAGATTATGCTTCGGGTG
CTTTCTTCAGCAAATATATCGAGCAAAGCTGGCAGCCAAAAACAGCAAGAGTACAGGCACTGTTTGCCGATGCCGGTATT
GCCATACCAAACCAACAAGATTGGATCGCGCTACGGCAATCCGTTATGGAACACGGTATTTATAATCAGAACCTACAGGC
GGTGCCACCAACAGGCTCTATTTCCTATATCAATCATTCGACATCAAGTATCCACCCGATTGTCTCTCCGATTGAGATCC
GTAAAGAAGGCAAAATAGGCCGTGTTTATTATCCCGCACCCTATATGACCAACGATAACCTTGAATATTATCAAGATGCC
TACCAGATCGGGCCTGAAAAAATCATCGATACCTATGCCGCAGCGACCCAACATGTTGATCAGGGCCTCTCTCTGACGCT
GTTCTTCCGTGATACCGCTACCACCCGCGATATTAACAAAGCACAGATTTACGCTTGGAAGAAAGGGATCAAGACCATTT
ATTACATCCGGCTACGTCAGATGGCATTAGAGGGAACCGAAGTCGAGGGCTGCGTTTCCTGTTCGCTGTAA

Upstream 100 bases:

>100_bases
ATATTATCTCCCACAAGTGTCAGGTTCCTTACCTGTATCGCTTTGAACTCCTAGGTACAGCAGAAGATGTGGCAAACGTT
CGCAAGGGAGTAACTGAATT

Downstream 100 bases:

>100_bases
TGTCTTTTCTGTTTACGGCAACTTTTCTGTTTACGGTAACTTTTCTGTTTACGGTAACTTTCCTGCTCACGGTAAATAGA
TAGCACTGAATTAATCAGGT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MATTKLNVIDSSDSELDYHSLNAMLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHNLQEKIDYLIEEGYYEKEVLD
QYDFSFIKSLFQQAYSKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLATGNQTLARELVEEIISGRFQ
PATPTFLNCGKKQRGELVSCFLLRIEDNMESIGRSINSALQLSKRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKML
EDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDIEQVY
GVPMSEISISEKYREMVDNKRIRKSKIKAREFFQILAEIQFESGYPYMMFEDTVNRANPIKGRINMSNLCSEILQVNAPT
EYHEDLSYRRIGKDISCNLGSLNIAQVMDSPDFGKSIEIAIRGLTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYL
ARERLFYGSEEALDFTNIYFYTVAYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYIEQSWQPKTARVQALFADAGI
AIPNQQDWIALRQSVMEHGIYNQNLQAVPPTGSISYINHSTSSIHPIVSPIEIRKEGKIGRVYYPAPYMTNDNLEYYQDA
YQIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL

Sequences:

>Translated_716_residues
MATTKLNVIDSSDSELDYHSLNAMLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHNLQEKIDYLIEEGYYEKEVLD
QYDFSFIKSLFQQAYSKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLATGNQTLARELVEEIISGRFQ
PATPTFLNCGKKQRGELVSCFLLRIEDNMESIGRSINSALQLSKRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKML
EDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDIEQVY
GVPMSEISISEKYREMVDNKRIRKSKIKAREFFQILAEIQFESGYPYMMFEDTVNRANPIKGRINMSNLCSEILQVNAPT
EYHEDLSYRRIGKDISCNLGSLNIAQVMDSPDFGKSIEIAIRGLTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYL
ARERLFYGSEEALDFTNIYFYTVAYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYIEQSWQPKTARVQALFADAGI
AIPNQQDWIALRQSVMEHGIYNQNLQAVPPTGSISYINHSTSSIHPIVSPIEIRKEGKIGRVYYPAPYMTNDNLEYYQDA
YQIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL
>Mature_715_residues
ATTKLNVIDSSDSELDYHSLNAMLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHNLQEKIDYLIEEGYYEKEVLDQ
YDFSFIKSLFQQAYSKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLATGNQTLARELVEEIISGRFQP
ATPTFLNCGKKQRGELVSCFLLRIEDNMESIGRSINSALQLSKRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKMLE
DAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDIEQVYG
VPMSEISISEKYREMVDNKRIRKSKIKAREFFQILAEIQFESGYPYMMFEDTVNRANPIKGRINMSNLCSEILQVNAPTE
YHEDLSYRRIGKDISCNLGSLNIAQVMDSPDFGKSIEIAIRGLTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYLA
RERLFYGSEEALDFTNIYFYTVAYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYIEQSWQPKTARVQALFADAGIA
IPNQQDWIALRQSVMEHGIYNQNLQAVPPTGSISYINHSTSSIHPIVSPIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAY
QIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=656, Percent_Identity=26.219512195122, Blast_Score=185, Evalue=2e-46,
Organism=Escherichia coli, GI1789030, Length=716, Percent_Identity=73.1843575418994, Blast_Score=1125, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=560, Percent_Identity=25.1785714285714, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17555242, Length=651, Percent_Identity=26.8817204301075, Blast_Score=191, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320914, Length=654, Percent_Identity=26.2996941896024, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322123, Length=654, Percent_Identity=25.9938837920489, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI17352481, Length=654, Percent_Identity=25.5351681957187, Blast_Score=172, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81689; Mature: 81558

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTKLNVIDSSDSELDYHSLNAMLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHN
CCCEEEEEECCCCCCCHHHHHHHHEEEEECCCEEEECHHHHHHHHHHHHHCCCCEEEEHH
LQEKIDYLIEEGYYEKEVLDQYDFSFIKSLFQQAYSKKFRFPTFLGAFKYYTSYTLKTFD
HHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECC
GKRYLERYEDRVCMVALTLATGNQTLARELVEEIISGRFQPATPTFLNCGKKQRGELVSC
CHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCCEEH
FLLRIEDNMESIGRSINSALQLSKRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKML
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHH
EDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFE
HHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEE
LAKNNEEMYLFSPYDIEQVYGVPMSEISISEKYREMVDNKRIRKSKIKAREFFQILAEIQ
EECCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FESGYPYMMFEDTVNRANPIKGRINMSNLCSEILQVNAPTEYHEDLSYRRIGKDISCNLG
CCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHHCCCCCCCCCC
SLNIAQVMDSPDFGKSIEIAIRGLTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYL
CCCHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHEEEECCCCHHHHH
ARERLFYGSEEALDFTNIYFYTVAYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYI
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EQSWQPKTARVQALFADAGIAIPNQQDWIALRQSVMEHGIYNQNLQAVPPTGSISYINHS
HHCCCCCHHHHEEEEECCCEECCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECC
TSSIHPIVSPIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYQIGPEKIIDTYAAATQHVD
CCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHC
QGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL
CCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure 
ATTKLNVIDSSDSELDYHSLNAMLNLYDADGRIQFDKDRLAARQYFLQHVNQNTVFFHN
CCEEEEEECCCCCCCHHHHHHHHEEEEECCCEEEECHHHHHHHHHHHHHCCCCEEEEHH
LQEKIDYLIEEGYYEKEVLDQYDFSFIKSLFQQAYSKKFRFPTFLGAFKYYTSYTLKTFD
HHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECC
GKRYLERYEDRVCMVALTLATGNQTLARELVEEIISGRFQPATPTFLNCGKKQRGELVSC
CHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCCEEH
FLLRIEDNMESIGRSINSALQLSKRGGGVAFLLSNIREVGAPIKRIENQSSGVIPIMKML
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHH
EDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKVRIKTLSLGVVIPDITFE
HHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEE
LAKNNEEMYLFSPYDIEQVYGVPMSEISISEKYREMVDNKRIRKSKIKAREFFQILAEIQ
EECCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FESGYPYMMFEDTVNRANPIKGRINMSNLCSEILQVNAPTEYHEDLSYRRIGKDISCNLG
CCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHHCCCCCCCCCC
SLNIAQVMDSPDFGKSIEIAIRGLTAVSEMSNIRSVPSIEKGNQESHAIGLGQMNLHGYL
CCCHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHEEEECCCCHHHHH
ARERLFYGSEEALDFTNIYFYTVAYHAIRASNQLAIERASHFKGFDESDYASGAFFSKYI
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EQSWQPKTARVQALFADAGIAIPNQQDWIALRQSVMEHGIYNQNLQAVPPTGSISYINHS
HHCCCCCHHHHEEEEECCCEECCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECC
TSSIHPIVSPIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYQIGPEKIIDTYAAATQHVD
CCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHC
QGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMALEGTEVEGCVSCSL
CCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]