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The map label for this gene is nrdF [H]

Identifier: 108811297

GI number: 108811297

Start: 1302086

End: 1303057

Strand: Reverse

Name: nrdF [H]

Synonym: YPN_1134

Alternate gene names: 108811297

Gene position: 1303057-1302086 (Counterclockwise)

Preceding gene: 108811298

Following gene: 108811296

Centisome position: 28.74

GC content: 42.7

Gene sequence:

>972_bases
ATGAACGTAGTAAAACCAATAACACGTATCAGCGCCATCAACTGGAATAAAATTGAAGACGATAAAGATCTGGAAGTGTG
GAACCGCCTGACATCTAATTTTTGGCTGCCGGAAAAAGTCCCGCTGTCGAATGATATTCCGTCGTGGGCGACACTCACGC
CACATGAACAACAACTGACTATCCGGGTTTTTACCGGCCTCACGCTATTGGATACGATCCAGAATACACTGGGCGCACCT
GCATTAATTAAAGATGCGATTACCCCCCATGAGGAAGCGATATTTTCAAATATCAGCTTTATGGAGGCCGTACACGCACG
TTCTTATAGCTCTATTTTTTCTACACTTTGTCTGACATCAGATGTAGACGATGCCTACCGTTGGAGTGAGGATAATGGGC
CACTCCAAAAGAAAGCTGATATTATTTCACAGCATTACCATAATGACGATCCGCTGAAGAAAAAAATAGCCAGTGTATTT
TTGGAATCATTTTTATTCTATTCCGGATTTTATTTACCCATGTATTGGTCCAGTAGAGCTAAATTAACCAACACCGCAGA
TCTTATTCGGCTTATTATTCGGGACGAAGCTGTCCATGGATATTATATCGGCTATAAGTTTCAAAAGGGGTTGGGAAAAA
TAGATAGTGCCCATCGTCAGGAAATAAAGAATTTCGCTTTCGACCTACTGCAAGATCTCTATGATAATGAAGTGCGTTAT
ACCGAAGATCTGTATGACCACGTTGGCTGGACAGAGGATGTAAAAAAATTTCTCCACTACAATGCCAATAAAGCACTCAT
GAATTTAGGCTATGAGGCATTATTTCCCGCCAGCCAGGCGGCGGTCAGCCCTGCAATTCTGGCCGCGTTGTCGCCAAACG
CAGATGAAAACCACGATTTTTTCTCCGGATCAGGTTCATCTTATGTGATCGGTAAAGCGGTCAATACTGAAGACGAAGAT
TGGGATTTTTAA

Upstream 100 bases:

>100_bases
TTCTGTTTACGGTAACTTTTCTGTTTACGGTAACTTTCCTGCTCACGGTAAATAGATAGCACTGAATTAATCAGGTCGGA
TAGATTAAGGAAACAATGAT

Downstream 100 bases:

>100_bases
GATTTCCCCACACGGCGGCCCTCCTTTTTAGTCTAGATAAAAAAGGAAATATTCTAGATAAAAAAGGGGGAGACTCAGCC
CCAATAGGCAGTTAGCCAAT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNVVKPITRISAINWNKIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPHEQQLTIRVFTGLTLLDTIQNTLGAP
ALIKDAITPHEEAIFSNISFMEAVHARSYSSIFSTLCLTSDVDDAYRWSEDNGPLQKKADIISQHYHNDDPLKKKIASVF
LESFLFYSGFYLPMYWSSRAKLTNTADLIRLIIRDEAVHGYYIGYKFQKGLGKIDSAHRQEIKNFAFDLLQDLYDNEVRY
TEDLYDHVGWTEDVKKFLHYNANKALMNLGYEALFPASQAAVSPAILAALSPNADENHDFFSGSGSSYVIGKAVNTEDED
WDF

Sequences:

>Translated_323_residues
MNVVKPITRISAINWNKIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPHEQQLTIRVFTGLTLLDTIQNTLGAP
ALIKDAITPHEEAIFSNISFMEAVHARSYSSIFSTLCLTSDVDDAYRWSEDNGPLQKKADIISQHYHNDDPLKKKIASVF
LESFLFYSGFYLPMYWSSRAKLTNTADLIRLIIRDEAVHGYYIGYKFQKGLGKIDSAHRQEIKNFAFDLLQDLYDNEVRY
TEDLYDHVGWTEDVKKFLHYNANKALMNLGYEALFPASQAAVSPAILAALSPNADENHDFFSGSGSSYVIGKAVNTEDED
WDF
>Mature_323_residues
MNVVKPITRISAINWNKIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPHEQQLTIRVFTGLTLLDTIQNTLGAP
ALIKDAITPHEEAIFSNISFMEAVHARSYSSIFSTLCLTSDVDDAYRWSEDNGPLQKKADIISQHYHNDDPLKKKIASVF
LESFLFYSGFYLPMYWSSRAKLTNTADLIRLIIRDEAVHGYYIGYKFQKGLGKIDSAHRQEIKNFAFDLLQDLYDNEVRY
TEDLYDHVGWTEDVKKFLHYNANKALMNLGYEALFPASQAAVSPAILAALSPNADENHDFFSGSGSSYVIGKAVNTEDED
WDF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=317, Percent_Identity=79.8107255520505, Blast_Score=534, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 36824; Mature: 36824

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVKPITRISAINWNKIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPHEQQLT
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
IRVFTGLTLLDTIQNTLGAPALIKDAITPHEEAIFSNISFMEAVHARSYSSIFSTLCLTS
EEHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVDDAYRWSEDNGPLQKKADIISQHYHNDDPLKKKIASVFLESFLFYSGFYLPMYWSSRA
CCCHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
KLTNTADLIRLIIRDEAVHGYYIGYKFQKGLGKIDSAHRQEIKNFAFDLLQDLYDNEVRY
CCCHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
TEDLYDHVGWTEDVKKFLHYNANKALMNLGYEALFPASQAAVSPAILAALSPNADENHDF
HHHHHHHCCCHHHHHHHHHCCCCHHHHHCCHHHHCCCHHHHHCHHHHHHCCCCCCCCCCC
FSGSGSSYVIGKAVNTEDEDWDF
CCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNVVKPITRISAINWNKIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPHEQQLT
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
IRVFTGLTLLDTIQNTLGAPALIKDAITPHEEAIFSNISFMEAVHARSYSSIFSTLCLTS
EEHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVDDAYRWSEDNGPLQKKADIISQHYHNDDPLKKKIASVFLESFLFYSGFYLPMYWSSRA
CCCHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
KLTNTADLIRLIIRDEAVHGYYIGYKFQKGLGKIDSAHRQEIKNFAFDLLQDLYDNEVRY
CCCHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
TEDLYDHVGWTEDVKKFLHYNANKALMNLGYEALFPASQAAVSPAILAALSPNADENHDF
HHHHHHHCCCHHHHHHHHHCCCCHHHHHCCHHHHCCCHHHHHCHHHHHHCCCCCCCCCCC
FSGSGSSYVIGKAVNTEDEDWDF
CCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2649479; 8195103 [H]