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The map label for this gene is lipA [H]

Identifier: 108811247

GI number: 108811247

Start: 1245117

End: 1246082

Strand: Reverse

Name: lipA [H]

Synonym: YPN_1083

Alternate gene names: 108811247

Gene position: 1246082-1245117 (Counterclockwise)

Preceding gene: 108811248

Following gene: 108811245

Centisome position: 27.48

GC content: 50.1

Gene sequence:

>966_bases
ATGAGTAAACCGATTCAGATGGAACGCGGCGTAAAATACCGTGATGCAGACAAAATGGCGTTAATCCCGGTAAAAAACGT
GGTGACCGAACGTCAGGAGCTGTTGCGTAAACCCGAGTGGCTAAAAATCAAGCTTCCGACTGATTCCAGCCGCATTCAGG
GTATCAAAGCTGCAATGCGTAAGAATGGTCTGCATTCGGTTTGTGAAGAGGCCTCCTGCCCTAACCTGTCTGAGTGCTTT
AACCACGGTACGGCAACATTTATGATCCTTGGTGCAATTTGTACCCGCCGTTGCCCATTCTGTGACGTTGCCCATGGTCG
CCCAGTCACACCGGATGCTAATGAGCCAGAGAAGCTGGCACAGACGATCCAAGACATGGGTCTGCGCTATGTTGTTATCA
CGTCCGTTGACCGTGATGACCTACGTGATGGTGGTGCTCAGCATTTTGCCGATTGTATTTCAGCGATCCGCGCCAAGAAT
CCAACCATCAAAATTGAAACGTTAGTCCCTGATTTCCGTGGTCGTATGGATCGCGCACTCGATATTCTGACCGCAACCCC
ACCAGATGTATTTAACCATAATTTGGAAAACGTACCACGTGTTTATCGGCAGGTTCGTCCAGGGGCGAACTACGATTGGT
CACTGAAATTACTTGAGCGCTTCAAAGAAGCACATCCAGATATCCCAACTAAATCCGGCTTAATGGTCGGCTTGGGCGAA
ACCAATGCCGAGATTGTCGAGGTCATGCATGATTTACGCCGCCACGGGGTCACGATGTTGACGCTGGGGCAATACTTGCA
ACCAAGCCGCCATCATTTACCCGTACAACGTTATGTCAGCCCGGCCGAGTTTGACGAAATGAAAGCAGAAGCCATGGCTA
TGGGCTTTACCCATGCAGCCTGTGGCCCATTTGTTCGCTCTTCTTACCATGCAGACCTGCAAGCGAAAGGTATGGAAGTG
AAGTAA

Upstream 100 bases:

>100_bases
TTTTAAACAATTTTCAAATAATTTTTAACGAACGCTACATTACGTTGAATTTAATGAAAAATCTCAAGTTGAGAGATTCA
GAACTGGAACCTGCCCGATT

Downstream 100 bases:

>100_bases
GCGGTAGGCTAAAGACTTCAGCCCATGGCTGAAATAACTTATTACACCTTAAAACAGCAGCCACCAAAAAACGGATTTCC
ATTGGAGATCCGTTTTTTTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKPIQMERGVKYRDADKMALIPVKNVVTERQELLRKPEWLKIKLPTDSSRIQGIKAAMRKNGLHSVCEEASCPNLSECF
NHGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLAQTIQDMGLRYVVITSVDRDDLRDGGAQHFADCISAIRAKN
PTIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRVYRQVRPGANYDWSLKLLERFKEAHPDIPTKSGLMVGLGE
TNAEIVEVMHDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPAEFDEMKAEAMAMGFTHAACGPFVRSSYHADLQAKGMEV
K

Sequences:

>Translated_321_residues
MSKPIQMERGVKYRDADKMALIPVKNVVTERQELLRKPEWLKIKLPTDSSRIQGIKAAMRKNGLHSVCEEASCPNLSECF
NHGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLAQTIQDMGLRYVVITSVDRDDLRDGGAQHFADCISAIRAKN
PTIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRVYRQVRPGANYDWSLKLLERFKEAHPDIPTKSGLMVGLGE
TNAEIVEVMHDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPAEFDEMKAEAMAMGFTHAACGPFVRSSYHADLQAKGMEV
K
>Mature_320_residues
SKPIQMERGVKYRDADKMALIPVKNVVTERQELLRKPEWLKIKLPTDSSRIQGIKAAMRKNGLHSVCEEASCPNLSECFN
HGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLAQTIQDMGLRYVVITSVDRDDLRDGGAQHFADCISAIRAKNP
TIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRVYRQVRPGANYDWSLKLLERFKEAHPDIPTKSGLMVGLGET
NAEIVEVMHDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPAEFDEMKAEAMAMGFTHAACGPFVRSSYHADLQAKGMEVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=44.0273037542662, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI37577164, Length=258, Percent_Identity=44.5736434108527, Blast_Score=224, Evalue=6e-59,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=92.2118380062305, Blast_Score=629, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=41.4634146341463, Blast_Score=220, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=43.5643564356436, Blast_Score=251, Evalue=7e-68,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=44.5993031358885, Blast_Score=244, Evalue=7e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36086; Mature: 35955

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIQMERGVKYRDADKMALIPVKNVVTERQELLRKPEWLKIKLPTDSSRIQGIKAAMR
CCCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
KNGLHSVCEEASCPNLSECFNHGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIQDMGLRYVVITSVDRDDLRDGGAQHFADCISAIRAKNPTIKIETLVPDFRGRMDRAL
HHHHHCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
DILTATPPDVFNHNLENVPRVYRQVRPGANYDWSLKLLERFKEAHPDIPTKSGLMVGLGE
HHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECC
TNAEIVEVMHDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPAEFDEMKAEAMAMGFTHAA
CHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAKGMEVK
HHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SKPIQMERGVKYRDADKMALIPVKNVVTERQELLRKPEWLKIKLPTDSSRIQGIKAAMR
CCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
KNGLHSVCEEASCPNLSECFNHGTATFMILGAICTRRCPFCDVAHGRPVTPDANEPEKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIQDMGLRYVVITSVDRDDLRDGGAQHFADCISAIRAKNPTIKIETLVPDFRGRMDRAL
HHHHHCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
DILTATPPDVFNHNLENVPRVYRQVRPGANYDWSLKLLERFKEAHPDIPTKSGLMVGLGE
HHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECC
TNAEIVEVMHDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPAEFDEMKAEAMAMGFTHAA
CHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAKGMEVK
HHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA