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The map label for this gene is thcD [H]

Identifier: 108797968

GI number: 108797968

Start: 1100825

End: 1102030

Strand: Direct

Name: thcD [H]

Synonym: Mmcs_0995

Alternate gene names: 108797968

Gene position: 1100825-1102030 (Clockwise)

Preceding gene: 108797967

Following gene: 108797972

Centisome position: 19.29

GC content: 67.91

Gene sequence:

>1206_bases
GTGTACAACGACCACGTGAGCACTTCAGGTGGCATCGTCATCGTCGGCGGCGGTCTCGCAGCGGCCCGTACCGCCGAGCA
ACTGCGGCGTTCGAACTATTCCGGCCCGGTCACGATCGTCAGCGACGAGGACCATCTACCGTACGACCGGCCGCCGCTGT
CGAAGGAAGTGCTGCGCAGCGAGACCGACGACGTCACGCTCAAGCCTGCGGAGTTCTACCAGGAAAACGACATCACCGTG
CTGCTCGGCGCCGGTGCGGCCAGCGTGGACACCGTCGGCAAGAAGGTCACGCTGACCGACGGGCGCGAGATCGGCTACGA
GGAGCTGGTGATCGCCACGGGGCTGGTGCCCAAGCGCATTCCGTCGTTCCCCGACCTCACCGGCATCCACGTGCTGCGCA
ACTTCGACGAGAGCCTCAAGCTGCGTGAGGAAGCCGGATCCGCGCGGAGCGCCGTGGTGATCGGCGCCGGCTTCATCGGC
TGCGAGGTCGCCGCGAGCCTGCGCAAACTCGGCGTCGACGTGGTGCTGGTCGAACCGCAGCCCGCCCCGTTGGCGTCGGT
GCTCGGCACGCAGATCGGCGAGCTGGTGGCCCGGCTGCACCGCGCCGAGGGCGTCGACGTGCGCTGCGGTGTCGGGGTGG
CCGAGGTCAAGGGTCAGGACGTCGACGGACAAGAGAAAGTGCAGTCGGTCGTATTGAGCGACGGCGCCGAGGTCGATGCC
GACCTCGTCGTGGTCGGAATCGGCTCGCGCCCGTCCACCGACTGGCTCGAGGACAGCGGCATCACGCTCGACAACGGCGT
GGTGTGCGACGCGGAGGGACGCTCGAGTGCACCGCACGTGTGGGCGATCGGCGACGTCGCCTCGTGGCTCGACACCATCG
GCAACCAAGTGCGCGTGGAACATTGGAGCAACGTCGCCGACCAGGCCCGAGTGCTGGTGCCCGCGATGCTCGGCCAGGAC
GTTCCGGGTGTGGTCTCGGTGCCGTACTTCTGGAGCGACCAGTACGACGTGAAGATCCAGTGCCTCGGCGAACCGGAGGC
CGACGACACCGTGCACATCGTCGAGGACGACGGCCGCAAATTCCTGGCGTACTACGAGCGCGACGGTGTGGTGGCCGGCG
TGGTCGGCGGCGGGATGCCCGGCAAGGTCATGAAGACCCGTAACAAGATCGCCAACGGCGCCCCGATCGCCGACGTGCTC
GGCTAG

Upstream 100 bases:

>100_bases
GAGCGCAGCGAGAGCATCGTGGTGCTGTCGAACCTCATCGAGCGCGCGCTGCCCTGAGTAACCCGAATCACAGGGTGGCG
TACCCCTCCGGCGACCCATC

Downstream 100 bases:

>100_bases
AACTGGCCGAGGTAGAAGCGGGCGCCCTGGTCGTCGACGCATTCGGCCATCAGGCCGTAGGGCTGGCGGTTCGGTTCCTG
TCGTACCGAACCACCGGCCT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MYNDHVSTSGGIVIVGGGLAAARTAEQLRRSNYSGPVTIVSDEDHLPYDRPPLSKEVLRSETDDVTLKPAEFYQENDITV
LLGAGAASVDTVGKKVTLTDGREIGYEELVIATGLVPKRIPSFPDLTGIHVLRNFDESLKLREEAGSARSAVVIGAGFIG
CEVAASLRKLGVDVVLVEPQPAPLASVLGTQIGELVARLHRAEGVDVRCGVGVAEVKGQDVDGQEKVQSVVLSDGAEVDA
DLVVVGIGSRPSTDWLEDSGITLDNGVVCDAEGRSSAPHVWAIGDVASWLDTIGNQVRVEHWSNVADQARVLVPAMLGQD
VPGVVSVPYFWSDQYDVKIQCLGEPEADDTVHIVEDDGRKFLAYYERDGVVAGVVGGGMPGKVMKTRNKIANGAPIADVL
G

Sequences:

>Translated_401_residues
MYNDHVSTSGGIVIVGGGLAAARTAEQLRRSNYSGPVTIVSDEDHLPYDRPPLSKEVLRSETDDVTLKPAEFYQENDITV
LLGAGAASVDTVGKKVTLTDGREIGYEELVIATGLVPKRIPSFPDLTGIHVLRNFDESLKLREEAGSARSAVVIGAGFIG
CEVAASLRKLGVDVVLVEPQPAPLASVLGTQIGELVARLHRAEGVDVRCGVGVAEVKGQDVDGQEKVQSVVLSDGAEVDA
DLVVVGIGSRPSTDWLEDSGITLDNGVVCDAEGRSSAPHVWAIGDVASWLDTIGNQVRVEHWSNVADQARVLVPAMLGQD
VPGVVSVPYFWSDQYDVKIQCLGEPEADDTVHIVEDDGRKFLAYYERDGVVAGVVGGGMPGKVMKTRNKIANGAPIADVL
G
>Mature_401_residues
MYNDHVSTSGGIVIVGGGLAAARTAEQLRRSNYSGPVTIVSDEDHLPYDRPPLSKEVLRSETDDVTLKPAEFYQENDITV
LLGAGAASVDTVGKKVTLTDGREIGYEELVIATGLVPKRIPSFPDLTGIHVLRNFDESLKLREEAGSARSAVVIGAGFIG
CEVAASLRKLGVDVVLVEPQPAPLASVLGTQIGELVARLHRAEGVDVRCGVGVAEVKGQDVDGQEKVQSVVLSDGAEVDA
DLVVVGIGSRPSTDWLEDSGITLDNGVVCDAEGRSSAPHVWAIGDVASWLDTIGNQVRVEHWSNVADQARVLVPAMLGQD
VPGVVSVPYFWSDQYDVKIQCLGEPEADDTVHIVEDDGRKFLAYYERDGVVAGVVGGGMPGKVMKTRNKIANGAPIADVL
G

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=396, Percent_Identity=32.0707070707071, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI21389617, Length=396, Percent_Identity=32.0707070707071, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI226437568, Length=396, Percent_Identity=32.0707070707071, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI22202629, Length=335, Percent_Identity=28.0597014925373, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI4757732, Length=335, Percent_Identity=28.0597014925373, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI195927006, Length=162, Percent_Identity=30.8641975308642, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788892, Length=385, Percent_Identity=32.2077922077922, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789065, Length=373, Percent_Identity=26.0053619302949, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789765, Length=284, Percent_Identity=26.7605633802817, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=379, Percent_Identity=30.3430079155673, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI32564386, Length=365, Percent_Identity=30.6849315068493, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585130, Length=363, Percent_Identity=27.5482093663912, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24639257, Length=369, Percent_Identity=29.2682926829268, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI281359715, Length=369, Percent_Identity=29.2682926829268, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI281359713, Length=369, Percent_Identity=29.2682926829268, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24639250, Length=369, Percent_Identity=29.2682926829268, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI18543267, Length=369, Percent_Identity=29.2682926829268, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24639252, Length=369, Percent_Identity=29.2682926829268, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24581020, Length=361, Percent_Identity=29.0858725761773, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI28573993, Length=361, Percent_Identity=29.0858725761773, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42424; Mature: 42424

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNDHVSTSGGIVIVGGGLAAARTAEQLRRSNYSGPVTIVSDEDHLPYDRPPLSKEVLRS
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHC
ETDDVTLKPAEFYQENDITVLLGAGAASVDTVGKKVTLTDGREIGYEELVIATGLVPKRI
CCCCEEECHHHHHCCCCEEEEEECCCCCHHHCCCEEEECCCCCCCHHHEEEECCCCHHHC
PSFPDLTGIHVLRNFDESLKLREEAGSARSAVVIGAGFIGCEVAASLRKLGVDVVLVEPQ
CCCCCCHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCC
PAPLASVLGTQIGELVARLHRAEGVDVRCGVGVAEVKGQDVDGQEKVQSVVLSDGAEVDA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCHHHHHHHHHHCCCCCCCC
DLVVVGIGSRPSTDWLEDSGITLDNGVVCDAEGRSSAPHVWAIGDVASWLDTIGNQVRVE
CEEEEECCCCCCCCCCCCCCCEECCCEEECCCCCCCCCEEEEHHHHHHHHHHCCCEEEEH
HWSNVADQARVLVPAMLGQDVPGVVSVPYFWSDQYDVKIQCLGEPEADDTVHIVEDDGRK
HHHCHHHHHHHHHHHHHCCCCCCEEECCEEECCCCCEEEEECCCCCCCCEEEEEECCCCE
FLAYYERDGVVAGVVGGGMPGKVMKTRNKIANGAPIADVLG
EEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MYNDHVSTSGGIVIVGGGLAAARTAEQLRRSNYSGPVTIVSDEDHLPYDRPPLSKEVLRS
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHC
ETDDVTLKPAEFYQENDITVLLGAGAASVDTVGKKVTLTDGREIGYEELVIATGLVPKRI
CCCCEEECHHHHHCCCCEEEEEECCCCCHHHCCCEEEECCCCCCCHHHEEEECCCCHHHC
PSFPDLTGIHVLRNFDESLKLREEAGSARSAVVIGAGFIGCEVAASLRKLGVDVVLVEPQ
CCCCCCHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCC
PAPLASVLGTQIGELVARLHRAEGVDVRCGVGVAEVKGQDVDGQEKVQSVVLSDGAEVDA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCHHHHHHHHHHCCCCCCCC
DLVVVGIGSRPSTDWLEDSGITLDNGVVCDAEGRSSAPHVWAIGDVASWLDTIGNQVRVE
CEEEEECCCCCCCCCCCCCCCEECCCEEECCCCCCCCCEEEEHHHHHHHHHHCCCEEEEH
HWSNVADQARVLVPAMLGQDVPGVVSVPYFWSDQYDVKIQCLGEPEADDTVHIVEDDGRK
HHHCHHHHHHHHHHHHHCCCCCCEEECCEEECCCCCEEEEECCCCCCCCEEEEEECCCCE
FLAYYERDGVVAGVVGGGMPGKVMKTRNKIANGAPIADVLG
EEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]