Click here to switch to the map view.

The map label for this gene is mcrB [H]

Identifier: 108797806

GI number: 108797806

Start: 917222

End: 919051

Strand: Reverse

Name: mcrB [H]

Synonym: Mmcs_0829

Alternate gene names: 108797806

Gene position: 919051-917222 (Counterclockwise)

Preceding gene: 108797807

Following gene: 108797805

Centisome position: 16.11

GC content: 60.05

Gene sequence:

>1830_bases
GTGGTGAGCAGTACGGAGGAGACGCCGCAGGTTCCCCCATGGCAGAAGTTCGCGCGTCCGGTACTGGAAGTGCTCAGTGA
CGGCACTGTCTGGCAGAGAAGTGCGCTCCATACGGCGGTGATGGACCGCCTCGGACTCTCGGCGGAGCAGCGAGCCGTCA
CACTGAACTCGGGCCAGGGACAGGCCGCAAATCGCGTCGGGTGGGCAGTGTCCTACTTGGCGCGAGCGGAGGCGGTGAAG
CGGGTTGCGAAAGGTCAGACGCAGATCACCGAGTTCGGCAGGCAGATCCTCCAGATGCATCCGGAGGAGATCACCCAAGC
CGATCTGGAAGCGGTGCCGGCCTTTCAGGCATATGTGCCCAAACAACATGCGACGCAGAAAGTCTCGATCGACACCGAGC
ACCTCCCTTCCTACTGGTTTGTCGGCGCCTTCTTCACCGACGAAGAGCATCACGAAGGGCCGGTCGGCGACCAGACAGAC
CGCTTCGTCGCCGAGGGACGTTGGGAGAACGGGAACAAGGGCAAGTACCAAGATCAGGTGAAAGTCATGAAGCCCGGCGA
CCACATAGCGATCAAGGCGGCCTTCACAAGAAAGCATGATCTCCCGTTCGCGAGTAAAGGTCATCCGGTGTCTGTCATGG
CGATCAAGGCGACCGGAATCATTGTCGAGAATGCCGGGGACGGGTACAACGTGACCGTCGAGTGGGATCCACCGGCACCC
GTGCGGGAGTGGTATTTCTACACGAGCCGCCTGACCGTCTGGCGCGTCAAGCCGACCGACTGGAAGACCAAGAACCTCAT
CGATTTCGCCTTCAACGACGCCGAACAAGACTACGACGCGTTCCGCAATACCGACCCCTGGGTGGAGCGCTTCGGTGACG
AAGCTCCCGAAGTCATCGACGAGGGTGACGACGAGACCATCGAGGAGGAGACGGCCACTTCGATCACCTACGGGATCAAG
AACATCATCGCCGACGGTTGTTTTCTGTCCGAGCAGACGCTCACCGGTTATCTCGATGCTGTGGAGCGCAAGAAGAACCT
CATCCTCCAAGGGCCACCTGGTACGGGTAAGACCTGGCTCGCCAAACGACTGGGACGCGCACTCATCGGTCAGGATTCCA
GCGACCTGCTTCAGTCGGTGCAATTCCACCCGACCCTGTCCTATGAGGACTTCGTCCGCGGCTGGCGCCCCGCCGGCGAC
GGACGTCTGTCGCTGGTTGACGGGCTGTTCCTCGACATCATCAAGAAGGCTGAGAGCAACCCCGGAAGCAAGTACATCCT
GGTAATCGAGGAGATCAATCGCGGGAACCTCGCCCAGATCTTCGGGGAGTTGCTCACGCTCCTGGAGGCCGACAAGCGCG
ATCCTGCCGAAGCACTGACCCTCACCTACCGCAGAACCGGAGAACCGCCCGTCTACATCCCTGAAAACTTGTACATCATC
GGCACGATGAATCTCGCCGACAGATCTCTGGCCATCGTCGACTTCGCCCTGCGCAGGAGGTTCGCATTTGCTGACCTTGC
ACCACAATTCAATTCGTCTTGGCGACAGTGGGTCTCGACCCGCAACGGTATCGATGAGGAGATCTTGGCCTCGCTGGCCG
AACGAATCGGCGTCTTGAACCAGAAGATTACCGAAGACCGCTCGTTGGGCGAGCAATATCGGATCGGACACAGCTTCTTC
ACTCCGGGGCACGATACGAAGGTCGACGACCCCTCTCAATGGATACGCCACGTCGTCGAACAGGAGATCCGCCCGCTCCT
TGCGGAGTATTGGTTCGACGATCCGCAGCGCGTCGAGGCCGAGAGTTCGGCCTTGATCGGCTCGATATGA

Upstream 100 bases:

>100_bases
TCCGAGCAGCGTTACGGCGCCTGCATGCCCGTCAGCCGAGGCAGTGGGCGGATTCTTCGCCGACCCGGCCTGCTCACAAC
CCGCACGGCGCTAGCGTGGA

Downstream 100 bases:

>100_bases
CGAAACCCCAGGCGATTATCAGTGGCAGCTCCGCCATTCCAGTGCGGAACCTCTGGCTGTTGATGTTGTATGCATCCCGG
CTCTATCAACGCAACCACCT

Product: ATPase

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MVSSTEETPQVPPWQKFARPVLEVLSDGTVWQRSALHTAVMDRLGLSAEQRAVTLNSGQGQAANRVGWAVSYLARAEAVK
RVAKGQTQITEFGRQILQMHPEEITQADLEAVPAFQAYVPKQHATQKVSIDTEHLPSYWFVGAFFTDEEHHEGPVGDQTD
RFVAEGRWENGNKGKYQDQVKVMKPGDHIAIKAAFTRKHDLPFASKGHPVSVMAIKATGIIVENAGDGYNVTVEWDPPAP
VREWYFYTSRLTVWRVKPTDWKTKNLIDFAFNDAEQDYDAFRNTDPWVERFGDEAPEVIDEGDDETIEEETATSITYGIK
NIIADGCFLSEQTLTGYLDAVERKKNLILQGPPGTGKTWLAKRLGRALIGQDSSDLLQSVQFHPTLSYEDFVRGWRPAGD
GRLSLVDGLFLDIIKKAESNPGSKYILVIEEINRGNLAQIFGELLTLLEADKRDPAEALTLTYRRTGEPPVYIPENLYII
GTMNLADRSLAIVDFALRRRFAFADLAPQFNSSWRQWVSTRNGIDEEILASLAERIGVLNQKITEDRSLGEQYRIGHSFF
TPGHDTKVDDPSQWIRHVVEQEIRPLLAEYWFDDPQRVEAESSALIGSI

Sequences:

>Translated_609_residues
MVSSTEETPQVPPWQKFARPVLEVLSDGTVWQRSALHTAVMDRLGLSAEQRAVTLNSGQGQAANRVGWAVSYLARAEAVK
RVAKGQTQITEFGRQILQMHPEEITQADLEAVPAFQAYVPKQHATQKVSIDTEHLPSYWFVGAFFTDEEHHEGPVGDQTD
RFVAEGRWENGNKGKYQDQVKVMKPGDHIAIKAAFTRKHDLPFASKGHPVSVMAIKATGIIVENAGDGYNVTVEWDPPAP
VREWYFYTSRLTVWRVKPTDWKTKNLIDFAFNDAEQDYDAFRNTDPWVERFGDEAPEVIDEGDDETIEEETATSITYGIK
NIIADGCFLSEQTLTGYLDAVERKKNLILQGPPGTGKTWLAKRLGRALIGQDSSDLLQSVQFHPTLSYEDFVRGWRPAGD
GRLSLVDGLFLDIIKKAESNPGSKYILVIEEINRGNLAQIFGELLTLLEADKRDPAEALTLTYRRTGEPPVYIPENLYII
GTMNLADRSLAIVDFALRRRFAFADLAPQFNSSWRQWVSTRNGIDEEILASLAERIGVLNQKITEDRSLGEQYRIGHSFF
TPGHDTKVDDPSQWIRHVVEQEIRPLLAEYWFDDPQRVEAESSALIGSI
>Mature_609_residues
MVSSTEETPQVPPWQKFARPVLEVLSDGTVWQRSALHTAVMDRLGLSAEQRAVTLNSGQGQAANRVGWAVSYLARAEAVK
RVAKGQTQITEFGRQILQMHPEEITQADLEAVPAFQAYVPKQHATQKVSIDTEHLPSYWFVGAFFTDEEHHEGPVGDQTD
RFVAEGRWENGNKGKYQDQVKVMKPGDHIAIKAAFTRKHDLPFASKGHPVSVMAIKATGIIVENAGDGYNVTVEWDPPAP
VREWYFYTSRLTVWRVKPTDWKTKNLIDFAFNDAEQDYDAFRNTDPWVERFGDEAPEVIDEGDDETIEEETATSITYGIK
NIIADGCFLSEQTLTGYLDAVERKKNLILQGPPGTGKTWLAKRLGRALIGQDSSDLLQSVQFHPTLSYEDFVRGWRPAGD
GRLSLVDGLFLDIIKKAESNPGSKYILVIEEINRGNLAQIFGELLTLLEADKRDPAEALTLTYRRTGEPPVYIPENLYII
GTMNLADRSLAIVDFALRRRFAFADLAPQFNSSWRQWVSTRNGIDEEILASLAERIGVLNQKITEDRSLGEQYRIGHSFF
TPGHDTKVDDPSQWIRHVVEQEIRPLLAEYWFDDPQRVEAESSALIGSI

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=294, Percent_Identity=42.1768707482993, Blast_Score=252, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 68578; Mature: 68578

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSTEETPQVPPWQKFARPVLEVLSDGTVWQRSALHTAVMDRLGLSAEQRAVTLNSGQG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCEEEEECCCC
QAANRVGWAVSYLARAEAVKRVAKGQTQITEFGRQILQMHPEEITQADLEAVPAFQAYVP
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHCC
KQHATQKVSIDTEHLPSYWFVGAFFTDEEHHEGPVGDQTDRFVAEGRWENGNKGKYQDQV
HHHCCCEEECCHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCE
KVMKPGDHIAIKAAFTRKHDLPFASKGHPVSVMAIKATGIIVENAGDGYNVTVEWDPPAP
EEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCEEEEEEECCCCC
VREWYFYTSRLTVWRVKPTDWKTKNLIDFAFNDAEQDYDAFRNTDPWVERFGDEAPEVID
HHHHEEEECEEEEEEECCCCCCCCCEEHHHHCCCHHHHHHHCCCCHHHHHHCCCCCHHHC
EGDDETIEEETATSITYGIKNIIADGCFLSEQTLTGYLDAVERKKNLILQGPPGTGKTWL
CCCCCHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCHHHH
AKRLGRALIGQDSSDLLQSVQFHPTLSYEDFVRGWRPAGDGRLSLVDGLFLDIIKKAESN
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PGSKYILVIEEINRGNLAQIFGELLTLLEADKRDPAEALTLTYRRTGEPPVYIPENLYII
CCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCCCEEECCCEEEE
GTMNLADRSLAIVDFALRRRFAFADLAPQFNSSWRQWVSTRNGIDEEILASLAERIGVLN
EECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKITEDRSLGEQYRIGHSFFTPGHDTKVDDPSQWIRHVVEQEIRPLLAEYWFDDPQRVEA
HHHHHHHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
ESSALIGSI
HHHHHCCCC
>Mature Secondary Structure
MVSSTEETPQVPPWQKFARPVLEVLSDGTVWQRSALHTAVMDRLGLSAEQRAVTLNSGQG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCEEEEECCCC
QAANRVGWAVSYLARAEAVKRVAKGQTQITEFGRQILQMHPEEITQADLEAVPAFQAYVP
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHCC
KQHATQKVSIDTEHLPSYWFVGAFFTDEEHHEGPVGDQTDRFVAEGRWENGNKGKYQDQV
HHHCCCEEECCHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCE
KVMKPGDHIAIKAAFTRKHDLPFASKGHPVSVMAIKATGIIVENAGDGYNVTVEWDPPAP
EEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCEEEEEEECCCCC
VREWYFYTSRLTVWRVKPTDWKTKNLIDFAFNDAEQDYDAFRNTDPWVERFGDEAPEVID
HHHHEEEECEEEEEEECCCCCCCCCEEHHHHCCCHHHHHHHCCCCHHHHHHCCCCCHHHC
EGDDETIEEETATSITYGIKNIIADGCFLSEQTLTGYLDAVERKKNLILQGPPGTGKTWL
CCCCCHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCHHHH
AKRLGRALIGQDSSDLLQSVQFHPTLSYEDFVRGWRPAGDGRLSLVDGLFLDIIKKAESN
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PGSKYILVIEEINRGNLAQIFGELLTLLEADKRDPAEALTLTYRRTGEPPVYIPENLYII
CCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCCCEEECCCEEEE
GTMNLADRSLAIVDFALRRRFAFADLAPQFNSSWRQWVSTRNGIDEEILASLAERIGVLN
EECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKITEDRSLGEQYRIGHSFFTPGHDTKVDDPSQWIRHVVEQEIRPLLAEYWFDDPQRVEA
HHHHHHHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
ESSALIGSI
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]