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The map label for this gene is ptrB [C]

Identifier: 108797603

GI number: 108797603

Start: 685874

End: 687865

Strand: Reverse

Name: ptrB [C]

Synonym: Mmcs_0623

Alternate gene names: 108797603

Gene position: 687865-685874 (Counterclockwise)

Preceding gene: 108797604

Following gene: 108797602

Centisome position: 12.06

GC content: 68.67

Gene sequence:

>1992_bases
GTGACGGTAGACGACGCGCACCTGTGGCTCGAGGACATCACCGGCGACGACGCCCTGGACTGGGTGCGACGGCACAACGA
ACCGACCCTGGCCGACCTGGGCGGTGAGCGCTTCGAGCAGATGCGCGCCGAGGCGCTCGAGGTGCTCGACACCGACGCCC
GCATCCCCTACGTCCGGCGCCGCGGTGAGTACCTCTACAACTTCTGGCGCGATGCGGCCAACCCGCGGGGGCTGTGGCGA
CGCACCACGCTGGAGAGCTACCGCACCGAGGAGCCCGACTGGGAGGTGGTCATCGACGTCGACGCACTCGCCCGCGCCGA
CGACGAGAACTGGGTGTGGGCGGGCGCCGACGTCATCGACCCCGACCACACCCGTGCGCTGATCAGCCTCTCGCGCGGCG
GTGCCGACGCCGCGATCGTGCGCGAATTCGACATGGTGTCAATGGAGTTCGTCGACGGCGGGTTCGAGCTGCCGGAGGCC
AAGACGGCGATCACGTGGGAGGACGAGGACACCGTCCTGGTGGGAACGGATTTCGGGGAGGGCGCCCTGACCGAATCCGG
TTATCCGAGGCTGGTCAAGCGGTGGCGTCGCGGTACGCCGCTCACCGAGGCCGAGACGGTCTACAGCGGCGAGCCCGCCG
ACGTCATCGTCACCGCGTCGGTCGATCGGACACCGGGCTTCGAGCGCACCGTGGTGCGCCGCGCCGTCGACTTCTTCAAC
GACGAGGTGTACGAGTTGCGCGCCGGCGAACTCAGCCGCATCGACGCCCCGACCGACGCGACCGTGTCGGCACACCGCGA
CTGGCTGCTCATCGAGCTGCGCAGCGACTGGGACGGCTACCGGGCAGGATCGCTGCTGGCCGCGAAATACGACGAATACC
TCGATGGCACAAGGGCTCTGCAGGTGGTGTTCGAACCCGATGAGCACACGTGCCTGCACCACTACGCGTGGACCAAGGAC
CGACTCGTGGTCGTCACGCTGGCCGACGTCGCGAGCCGCGTCGAGGTGTACACCCCCGGCGAGTGGACGGCGCAGCCCGT
GCCGGGACTGCCGGACAACACCAACACGGTGATCGTGGCGGCCGACGACCTGGGCGACGAGATCTTCCTGGACTCCAGCG
GTTTCGACACCCCGTCGCGGCTCCTGCAGGGCGCGGCCGGCGGTGAACTCACCGAGATCAAGCGGGCGCCGTCGTTCTTC
GACGCCGCCGATCTCAAGGTCGACCAGCACTTCGCGACATCAGCCGACGGCACCAAGATCCCGTACTTCGTTGTCGGCCA
CCGGCATCAGCAGGCGCCCGGGCCGACGCTGCTGGGCGGTTACGGCGGGTTCGAGGTCGCGCGCACACCCGGTTACGACG
GTGTGCTCGGCCGGCTCTGGTTGTCTCGGGGCGGCACCTACGTGCTGGCCAACATCCGCGGCGGCGGGGAGTACGGACCG
ACGTGGCATACGCAGGCGATGCGCGAGGGCCGCCACCTGGTGGGTGAGGACTTCGCCGCCGTCGCAGCCGATCTCGTCGA
ACGCGGAATCACGACGGTCGACCGGTTGGGCGCGCAGGGCGGCAGCAACGGCGGGCTGCTGATGGGGATCATGCTCACGC
AGTACCCGGAGTTGTTCGGCGCGCTGGTCTGCAGCGTGCCGCTGCTCGACATGCGCCGGTTCCACCTGCTGCTCGCCGGG
GCGTCCTGGGTGGCCGAGTACGGCAACCCGGATGACCCGGACGACTGGGAGTTCATCTCGAAATACTCTCCCTATCAGAA
CATCTCGGCCGAGCGCCGATACCCGCCGGTGCTGATCACCACCTCCACACGCGACGACCGCGTGCATCCGGGACATGCGC
GCAAGATGACCGCAGCGCTCGAGGATGCCGGACAGCCGGTGCAGTACTACGAGAACATCGAGGGTGGGCACGGCGGCGCC
GCGGACAATTCGCAGGCTGCGTTCCGCGCGGCGCTGATCTACGAGTTCCTGTGGCGGAAGCTGGGCGGATAG

Upstream 100 bases:

>100_bases
GCTACAGCTCAAGGCCCGCTTCGAAGGCATCCCGACACCCGTGTACGGACTGCAGGAGGTGCACCACCTGAGCTGACGCC
CCACGGGCGACAATGAATGG

Downstream 100 bases:

>100_bases
GCGTTCACCGGCGACAGCACCGCGGGTTGTCACCCGTGTCATCTCTCAGGACATCGGTGACAGTTCTGCATCAGGACATC
GGTGACGGTTCTGGTGGTCT

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 663; Mature: 662

Protein sequence:

>663_residues
MTVDDAHLWLEDITGDDALDWVRRHNEPTLADLGGERFEQMRAEALEVLDTDARIPYVRRRGEYLYNFWRDAANPRGLWR
RTTLESYRTEEPDWEVVIDVDALARADDENWVWAGADVIDPDHTRALISLSRGGADAAIVREFDMVSMEFVDGGFELPEA
KTAITWEDEDTVLVGTDFGEGALTESGYPRLVKRWRRGTPLTEAETVYSGEPADVIVTASVDRTPGFERTVVRRAVDFFN
DEVYELRAGELSRIDAPTDATVSAHRDWLLIELRSDWDGYRAGSLLAAKYDEYLDGTRALQVVFEPDEHTCLHHYAWTKD
RLVVVTLADVASRVEVYTPGEWTAQPVPGLPDNTNTVIVAADDLGDEIFLDSSGFDTPSRLLQGAAGGELTEIKRAPSFF
DAADLKVDQHFATSADGTKIPYFVVGHRHQQAPGPTLLGGYGGFEVARTPGYDGVLGRLWLSRGGTYVLANIRGGGEYGP
TWHTQAMREGRHLVGEDFAAVAADLVERGITTVDRLGAQGGSNGGLLMGIMLTQYPELFGALVCSVPLLDMRRFHLLLAG
ASWVAEYGNPDDPDDWEFISKYSPYQNISAERRYPPVLITTSTRDDRVHPGHARKMTAALEDAGQPVQYYENIEGGHGGA
ADNSQAAFRAALIYEFLWRKLGG

Sequences:

>Translated_663_residues
MTVDDAHLWLEDITGDDALDWVRRHNEPTLADLGGERFEQMRAEALEVLDTDARIPYVRRRGEYLYNFWRDAANPRGLWR
RTTLESYRTEEPDWEVVIDVDALARADDENWVWAGADVIDPDHTRALISLSRGGADAAIVREFDMVSMEFVDGGFELPEA
KTAITWEDEDTVLVGTDFGEGALTESGYPRLVKRWRRGTPLTEAETVYSGEPADVIVTASVDRTPGFERTVVRRAVDFFN
DEVYELRAGELSRIDAPTDATVSAHRDWLLIELRSDWDGYRAGSLLAAKYDEYLDGTRALQVVFEPDEHTCLHHYAWTKD
RLVVVTLADVASRVEVYTPGEWTAQPVPGLPDNTNTVIVAADDLGDEIFLDSSGFDTPSRLLQGAAGGELTEIKRAPSFF
DAADLKVDQHFATSADGTKIPYFVVGHRHQQAPGPTLLGGYGGFEVARTPGYDGVLGRLWLSRGGTYVLANIRGGGEYGP
TWHTQAMREGRHLVGEDFAAVAADLVERGITTVDRLGAQGGSNGGLLMGIMLTQYPELFGALVCSVPLLDMRRFHLLLAG
ASWVAEYGNPDDPDDWEFISKYSPYQNISAERRYPPVLITTSTRDDRVHPGHARKMTAALEDAGQPVQYYENIEGGHGGA
ADNSQAAFRAALIYEFLWRKLGG
>Mature_662_residues
TVDDAHLWLEDITGDDALDWVRRHNEPTLADLGGERFEQMRAEALEVLDTDARIPYVRRRGEYLYNFWRDAANPRGLWRR
TTLESYRTEEPDWEVVIDVDALARADDENWVWAGADVIDPDHTRALISLSRGGADAAIVREFDMVSMEFVDGGFELPEAK
TAITWEDEDTVLVGTDFGEGALTESGYPRLVKRWRRGTPLTEAETVYSGEPADVIVTASVDRTPGFERTVVRRAVDFFND
EVYELRAGELSRIDAPTDATVSAHRDWLLIELRSDWDGYRAGSLLAAKYDEYLDGTRALQVVFEPDEHTCLHHYAWTKDR
LVVVTLADVASRVEVYTPGEWTAQPVPGLPDNTNTVIVAADDLGDEIFLDSSGFDTPSRLLQGAAGGELTEIKRAPSFFD
AADLKVDQHFATSADGTKIPYFVVGHRHQQAPGPTLLGGYGGFEVARTPGYDGVLGRLWLSRGGTYVLANIRGGGEYGPT
WHTQAMREGRHLVGEDFAAVAADLVERGITTVDRLGAQGGSNGGLLMGIMLTQYPELFGALVCSVPLLDMRRFHLLLAGA
SWVAEYGNPDDPDDWEFISKYSPYQNISAERRYPPVLITTSTRDDRVHPGHARKMTAALEDAGQPVQYYENIEGGHGGAA
DNSQAAFRAALIYEFLWRKLGG

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=365, Percent_Identity=32.0547945205479, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1788150, Length=299, Percent_Identity=30.4347826086957, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24583414, Length=369, Percent_Identity=35.5013550135501, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI221510989, Length=326, Percent_Identity=35.2760736196319, Blast_Score=181, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 73525; Mature: 73394

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00214 FABP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDDAHLWLEDITGDDALDWVRRHNEPTLADLGGERFEQMRAEALEVLDTDARIPYVRR
CCCCCHHHHHHHCCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHH
RGEYLYNFWRDAANPRGLWRRTTLESYRTEEPDWEVVIDVDALARADDENWVWAGADVID
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCEEEECCCCCC
PDHTRALISLSRGGADAAIVREFDMVSMEFVDGGFELPEAKTAITWEDEDTVLVGTDFGE
CCCCEEEEEEECCCCCEEHHHHHHHEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCC
GALTESGYPRLVKRWRRGTPLTEAETVYSGEPADVIVTASVDRTPGFERTVVRRAVDFFN
CCCCCCCCHHHHHHHHCCCCCCCCHHEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHC
DEVYELRAGELSRIDAPTDATVSAHRDWLLIELRSDWDGYRAGSLLAAKYDEYLDGTRAL
CCCEEECCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEE
QVVFEPDEHTCLHHYAWTKDRLVVVTLADVASRVEVYTPGEWTAQPVPGLPDNTNTVIVA
EEEECCCCCCEEEHEECCCCCEEEEEHHHHHHCEEEECCCCCCCCCCCCCCCCCCEEEEE
ADDLGDEIFLDSSGFDTPSRLLQGAAGGELTEIKRAPSFFDAADLKVDQHFATSADGTKI
ECCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCHHCCEECHHCCCCCCCCCC
PYFVVGHRHQQAPGPTLLGGYGGFEVARTPGYDGVLGRLWLSRGGTYVLANIRGGGEYGP
CEEEEECCCCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCC
TWHTQAMREGRHLVGEDFAAVAADLVERGITTVDRLGAQGGSNGGLLMGIMLTQYPELFG
CHHHHHHHCCCHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
ALVCSVPLLDMRRFHLLLAGASWVAEYGNPDDPDDWEFISKYSPYQNISAERRYPPVLIT
HHHHHCCHHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEE
TSTRDDRVHPGHARKMTAALEDAGQPVQYYENIEGGHGGAADNSQAAFRAALIYEFLWRK
ECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LGG
HCC
>Mature Secondary Structure 
TVDDAHLWLEDITGDDALDWVRRHNEPTLADLGGERFEQMRAEALEVLDTDARIPYVRR
CCCCHHHHHHHCCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHH
RGEYLYNFWRDAANPRGLWRRTTLESYRTEEPDWEVVIDVDALARADDENWVWAGADVID
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCEEEECCCCCC
PDHTRALISLSRGGADAAIVREFDMVSMEFVDGGFELPEAKTAITWEDEDTVLVGTDFGE
CCCCEEEEEEECCCCCEEHHHHHHHEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCC
GALTESGYPRLVKRWRRGTPLTEAETVYSGEPADVIVTASVDRTPGFERTVVRRAVDFFN
CCCCCCCCHHHHHHHHCCCCCCCCHHEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHC
DEVYELRAGELSRIDAPTDATVSAHRDWLLIELRSDWDGYRAGSLLAAKYDEYLDGTRAL
CCCEEECCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEE
QVVFEPDEHTCLHHYAWTKDRLVVVTLADVASRVEVYTPGEWTAQPVPGLPDNTNTVIVA
EEEECCCCCCEEEHEECCCCCEEEEEHHHHHHCEEEECCCCCCCCCCCCCCCCCCEEEEE
ADDLGDEIFLDSSGFDTPSRLLQGAAGGELTEIKRAPSFFDAADLKVDQHFATSADGTKI
ECCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCHHCCEECHHCCCCCCCCCC
PYFVVGHRHQQAPGPTLLGGYGGFEVARTPGYDGVLGRLWLSRGGTYVLANIRGGGEYGP
CEEEEECCCCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCC
TWHTQAMREGRHLVGEDFAAVAADLVERGITTVDRLGAQGGSNGGLLMGIMLTQYPELFG
CHHHHHHHCCCHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
ALVCSVPLLDMRRFHLLLAGASWVAEYGNPDDPDDWEFISKYSPYQNISAERRYPPVLIT
HHHHHCCHHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEE
TSTRDDRVHPGHARKMTAALEDAGQPVQYYENIEGGHGGAADNSQAAFRAALIYEFLWRK
ECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LGG
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]