Click here to switch to the map view.

The map label for this gene is pimE [H]

Identifier: 108797446

GI number: 108797446

Start: 520127

End: 521443

Strand: Direct

Name: pimE [H]

Synonym: Mmcs_0466

Alternate gene names: 108797446

Gene position: 520127-521443 (Clockwise)

Preceding gene: 108797445

Following gene: 108797447

Centisome position: 9.12

GC content: 61.5

Gene sequence:

>1317_bases
GTGGCTTCGATCTCTATGGAACGTTCAACCGTCGCGCGAGGTGTCATGACGCGCCGATGTCGCCATCTGACTCGGGTGGC
GCCGTGGTTGCTCGCCGTCAGCCTCGCATTACGATTCGTTGGCTCGATACCGTTCCTGGATATGCTGTTCGTCGATTTGC
GGGTGTACATACTCGGTGGCGAAGCATTGACCCAGCCCGGCACGCTGTACGGACTAACTTACATTGATAGCTATGGCGAT
CATCTGCCATTCATCTATCCACCATTCGCTGCTCTGCTTTTCTACCCGTTGCACTTTCTTCCGTTCCCCGTAGTAGCGTG
GATCTGGCAACTCGCCGTCGTAGCCGGTCTGTACGGTCTGGTACGGATTAGTCAGCGAATGTTGGGTGGCGGTAGTCACC
GGGTCGCCATGCTCTGGACCGCAGCGGGAATTTGGTTCGAGCCCATCCGAACCATACTGACCTGTTCCCAAGTAGGCGTC
TTCCTTACGCTGGCCGTGTTGTATGCGGCATACACGTCGCGGTCGTGGTTGTCGGGACTGCTGGTGGGCCTATCCGCTGG
CGTGAAGGTGACACCGGCGATCACCGGTCTGTACTTCGTCGGAATGCGACGCTGGGCGGCGGTCTGTTTCTCGCTGGCTG
CATTTCTGGCCACGGTATTGGTGTCGGCGGTGATAGCTCCGCAGGAGACTCGTCAGTTCTTCGGCGGATTATTCAGTCGG
GTTCCGATGCCGACAGCCACCTGCTTGAACCAGTCGTGGCGGGGCACGGTGTCGCGCATTGTCGGCTACGATGCCGGCCG
TTCGTTGGTGCTGCTTGTGGCGATCGGGGTAACGGCAGTGTTGGCGCTCCTGGCATGGCGGGAGCTGGGTAGGGGTCCGG
GTGGGCGGGATGCGCTTGCTTCGTTATTGGTCGTACAACTGTTCGGGCTCACGGCTTCACCAATCTCGTGGGCCCACCAC
TGGGTATGGGTCGTTCCACTGACGATCTGGCTTCTCCATGGTCCATGGCGGGGCAGAGCCGGTGCCCGTGTCCTCGGTAG
CGGGTGGATCGCCTTGATGGTGATCGGTGTGCCGACATTGCTGTCCGGTCAGCAGGCCGACCTGTCGACCTTCTCACGCC
CGTGGTACCTCGCCGTGGCCGCTGCCGTCTACGTGCCGATGACCCTAGTCACCTTTGCCTGGATCATCGTCGCCAGCCGA
ACGGGGACCCGGCGTACCTGTCCGCCGTCGGAGCCGAAGGCCGAAAGCGCACCGTCGACTTCGGCGGTGGTGTTCACGCC
CGCGGCGTCGCATGCGGAGCGTCCAACGAACCGATGA

Upstream 100 bases:

>100_bases
GCTATCGGCCGCGCTACTGGGAGAGCAGTGGAACGGCCGAGGATTAAGTGGGGCGGGGGCAACGCTGACCACCAGGCACT
CAGCGGTAGGGGGAGGACGC

Downstream 100 bases:

>100_bases
CTCGACTGCCCTTGCCGATACCGCGCTCGGACGGAGCGAAAGCAGCACCGACCGTCGGTCGTTATAGTCGCCCGATGCGC
GCGGCGTCGGCTTTGAAGGT

Product: mannosyltransferase

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Alpha-manT; PPM-dependent mannosyltransferase; Polyprenol-phosphate-mannose alpha-mannosyltransferase; PPM alpha-mannosyltransferase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MASISMERSTVARGVMTRRCRHLTRVAPWLLAVSLALRFVGSIPFLDMLFVDLRVYILGGEALTQPGTLYGLTYIDSYGD
HLPFIYPPFAALLFYPLHFLPFPVVAWIWQLAVVAGLYGLVRISQRMLGGGSHRVAMLWTAAGIWFEPIRTILTCSQVGV
FLTLAVLYAAYTSRSWLSGLLVGLSAGVKVTPAITGLYFVGMRRWAAVCFSLAAFLATVLVSAVIAPQETRQFFGGLFSR
VPMPTATCLNQSWRGTVSRIVGYDAGRSLVLLVAIGVTAVLALLAWRELGRGPGGRDALASLLVVQLFGLTASPISWAHH
WVWVVPLTIWLLHGPWRGRAGARVLGSGWIALMVIGVPTLLSGQQADLSTFSRPWYLAVAAAVYVPMTLVTFAWIIVASR
TGTRRTCPPSEPKAESAPSTSAVVFTPAASHAERPTNR

Sequences:

>Translated_438_residues
MASISMERSTVARGVMTRRCRHLTRVAPWLLAVSLALRFVGSIPFLDMLFVDLRVYILGGEALTQPGTLYGLTYIDSYGD
HLPFIYPPFAALLFYPLHFLPFPVVAWIWQLAVVAGLYGLVRISQRMLGGGSHRVAMLWTAAGIWFEPIRTILTCSQVGV
FLTLAVLYAAYTSRSWLSGLLVGLSAGVKVTPAITGLYFVGMRRWAAVCFSLAAFLATVLVSAVIAPQETRQFFGGLFSR
VPMPTATCLNQSWRGTVSRIVGYDAGRSLVLLVAIGVTAVLALLAWRELGRGPGGRDALASLLVVQLFGLTASPISWAHH
WVWVVPLTIWLLHGPWRGRAGARVLGSGWIALMVIGVPTLLSGQQADLSTFSRPWYLAVAAAVYVPMTLVTFAWIIVASR
TGTRRTCPPSEPKAESAPSTSAVVFTPAASHAERPTNR
>Mature_437_residues
ASISMERSTVARGVMTRRCRHLTRVAPWLLAVSLALRFVGSIPFLDMLFVDLRVYILGGEALTQPGTLYGLTYIDSYGDH
LPFIYPPFAALLFYPLHFLPFPVVAWIWQLAVVAGLYGLVRISQRMLGGGSHRVAMLWTAAGIWFEPIRTILTCSQVGVF
LTLAVLYAAYTSRSWLSGLLVGLSAGVKVTPAITGLYFVGMRRWAAVCFSLAAFLATVLVSAVIAPQETRQFFGGLFSRV
PMPTATCLNQSWRGTVSRIVGYDAGRSLVLLVAIGVTAVLALLAWRELGRGPGGRDALASLLVVQLFGLTASPISWAHHW
VWVVPLTIWLLHGPWRGRAGARVLGSGWIALMVIGVPTLLSGQQADLSTFSRPWYLAVAAAVYVPMTLVTFAWIIVASRT
GTRRTCPPSEPKAESAPSTSAVVFTPAASHAERPTNR

Specific function: Catalyzes the alpha-1,2 addition of a mannose residue from polyprenol-phosphate-mannose (PPM) to a monoacyl phosphatidylinositol pentamannoside (AcPIM5) to generate a monoacyl phosphatidylinositol hexamannoside (AcPIM6) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PIGM family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018584 [H]

Pfam domain/function: PF09594 DUF2029 [H]

EC number: NA

Molecular weight: Translated: 47570; Mature: 47438

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEECCHHHCCCCCCC
>Mature Secondary Structure 
ASISMERSTVARGVMTRRCRHLTRVAPWLLAVSLALRFVGSIPFLDMLFVDLRVYILGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECC
EALTQPGTLYGLTYIDSYGDHLPFIYPPFAALLFYPLHFLPFPVVAWIWQLAVVAGLYGL
HHHCCCCCEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRISQRMLGGGSHRVAMLWTAAGIWFEPIRTILTCSQVGVFLTLAVLYAAYTSRSWLSGL
HHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVGLSAGVKVTPAITGLYFVGMRRWAAVCFSLAAFLATVLVSAVIAPQETRQFFGGLFSR
HHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VPMPTATCLNQSWRGTVSRIVGYDAGRSLVLLVAIGVTAVLALLAWRELGRGPGGRDALA
CCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SLLVVQLFGLTASPISWAHHWVWVVPLTIWLLHGPWRGRAGARVLGSGWIALMVIGVPTL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHCCHHHHHHHHHHHH
LSGQQADLSTFSRPWYLAVAAAVYVPMTLVTFAWIIVASRTGTRRTCPPSEPKAESAPST
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SAVVFTPAASHAERPTNR
CEEEEECCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]