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The map label for this gene is pimE [H]

Identifier: 108797447

GI number: 108797447

Start: 521440

End: 522726

Strand: Direct

Name: pimE [H]

Synonym: Mmcs_0467

Alternate gene names: 108797447

Gene position: 521440-522726 (Clockwise)

Preceding gene: 108797446

Following gene: 108797448

Centisome position: 9.14

GC content: 64.72

Gene sequence:

>1287_bases
ATGACTCGACTGCCCTTGCCGATACCGCGCTCGGACGGAGCGAAAGCAGCACCGACCGTCGGTCGTTATAGTCGCCCGAT
GCGCGCGGCGTCGGCTTTGAAGGTGCGCCCCTGTCTCGCCCGCGTGGTGGCCCTGGCTCCGATCTTGCTCGCCGTCAGCA
TCATTGCGCGACTCGCCTCGACGATGGTGTATTACTTCTTCGCCGGCGACGCCTTCTTCGACCTGCACGTGTACGTGATC
GGGGGTGCGGCGCTCAACCACCCCGGCACCCTGTACGACGTCGTCTACGTCGATCCGACCAAGGGCGAGCGGCTGCCGTT
CACCTATCCTGCGTTTGCGGCGATGGTGTTCTATCCACTGCACCTCCTGCCGTTCGGTCTGCTCGCGTTCCTCTGGCAGA
TTGCGCTGATCGCCGCGATCTACGCGTCAATACGCATCAGCCAGCGCCTGATCGGGGACGGTGCTGGCGGACCCCGGGTC
GCCATGTTGTGGACGGCCGTCGCCATCTGGCTAGAGCCTGTAGCCAGCAGCGTCCAGACGGGTCAGGTCGGCGTCTTCTT
GATGCTTGCCGTGCTGTACGCCGCATACAGCTCGCGGTGGTGGTTGTCTGGTCTGTTGGTCGGCGCGGGCGCAGGGGTGA
AGCTGACACCAGCGATCACCGGCCTTTACTTCGTCGGCATGAGGCGCTGGGCGGCAGCGGCGTTCTCGGCCGTGACTTTT
TTCGCCACCGTCGGGCTGGCCTACGTCGCCCTGCCCGCCGACGCGGGTCGCTACTTTCCTGACCAAATGACGGAAGCGGG
CACGACGCTGCCGATCGGATCGTCGTGGAACCAGTCCTGGCTGGGTGGACTCTCGCGGATCCTGGGTTATGACCCGGGGT
TACATCCCCTAGTCATCGCAGCGGTCGCGGTGACCGCATTGCTGGCTGCCCTCGCATGGCGGGCGCTGGGCTCCGCAGAT
GGACGGGACCGACTGGCATCACTGCTGGTGGTCCAATTCCTCGGACTGATGGCGTCGCCGGTGTCGTGGATTCATCATTG
GGTGTGGGTGATCCCTCTGATCATCTGGCTGATCCACGGGCCTTGGCGCGAAAGGGCCGGCGCACGTCTCCTCGGCCTGG
GTTGGTTGGCGCTCATGGTGATCAGCGTTCCCAGCCTGCTGTCTCTCGCCCAACCTGACATCTGGGAGATCAGCCGGCCC
TGGTACCTTGCGTGGGCTGAAATGGTCTACATCGTCGCGTCGTTGTCGACGCTGGCCTGGATCATCAGAACCGGGCGCCG
TGCCTGA

Upstream 100 bases:

>100_bases
GTACCTGTCCGCCGTCGGAGCCGAAGGCCGAAAGCGCACCGTCGACTTCGGCGGTGGTGTTCACGCCCGCGGCGTCGCAT
GCGGAGCGTCCAACGAACCG

Downstream 100 bases:

>100_bases
GTCGGGAGTGCCGGCCGGGCGCCGGCGACTTCTGGCCCCGCTGTTGCTGGTCACCAGCGCCGCGGCGCTGCTTGCCGTGA
CCATGGCCAATCCGCCCGAT

Product: mannosyltransferase

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Alpha-manT; PPM-dependent mannosyltransferase; Polyprenol-phosphate-mannose alpha-mannosyltransferase; PPM alpha-mannosyltransferase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTRLPLPIPRSDGAKAAPTVGRYSRPMRAASALKVRPCLARVVALAPILLAVSIIARLASTMVYYFFAGDAFFDLHVYVI
GGAALNHPGTLYDVVYVDPTKGERLPFTYPAFAAMVFYPLHLLPFGLLAFLWQIALIAAIYASIRISQRLIGDGAGGPRV
AMLWTAVAIWLEPVASSVQTGQVGVFLMLAVLYAAYSSRWWLSGLLVGAGAGVKLTPAITGLYFVGMRRWAAAAFSAVTF
FATVGLAYVALPADAGRYFPDQMTEAGTTLPIGSSWNQSWLGGLSRILGYDPGLHPLVIAAVAVTALLAALAWRALGSAD
GRDRLASLLVVQFLGLMASPVSWIHHWVWVIPLIIWLIHGPWRERAGARLLGLGWLALMVISVPSLLSLAQPDIWEISRP
WYLAWAEMVYIVASLSTLAWIIRTGRRA

Sequences:

>Translated_428_residues
MTRLPLPIPRSDGAKAAPTVGRYSRPMRAASALKVRPCLARVVALAPILLAVSIIARLASTMVYYFFAGDAFFDLHVYVI
GGAALNHPGTLYDVVYVDPTKGERLPFTYPAFAAMVFYPLHLLPFGLLAFLWQIALIAAIYASIRISQRLIGDGAGGPRV
AMLWTAVAIWLEPVASSVQTGQVGVFLMLAVLYAAYSSRWWLSGLLVGAGAGVKLTPAITGLYFVGMRRWAAAAFSAVTF
FATVGLAYVALPADAGRYFPDQMTEAGTTLPIGSSWNQSWLGGLSRILGYDPGLHPLVIAAVAVTALLAALAWRALGSAD
GRDRLASLLVVQFLGLMASPVSWIHHWVWVIPLIIWLIHGPWRERAGARLLGLGWLALMVISVPSLLSLAQPDIWEISRP
WYLAWAEMVYIVASLSTLAWIIRTGRRA
>Mature_427_residues
TRLPLPIPRSDGAKAAPTVGRYSRPMRAASALKVRPCLARVVALAPILLAVSIIARLASTMVYYFFAGDAFFDLHVYVIG
GAALNHPGTLYDVVYVDPTKGERLPFTYPAFAAMVFYPLHLLPFGLLAFLWQIALIAAIYASIRISQRLIGDGAGGPRVA
MLWTAVAIWLEPVASSVQTGQVGVFLMLAVLYAAYSSRWWLSGLLVGAGAGVKLTPAITGLYFVGMRRWAAAAFSAVTFF
ATVGLAYVALPADAGRYFPDQMTEAGTTLPIGSSWNQSWLGGLSRILGYDPGLHPLVIAAVAVTALLAALAWRALGSADG
RDRLASLLVVQFLGLMASPVSWIHHWVWVIPLIIWLIHGPWRERAGARLLGLGWLALMVISVPSLLSLAQPDIWEISRPW
YLAWAEMVYIVASLSTLAWIIRTGRRA

Specific function: Catalyzes the alpha-1,2 addition of a mannose residue from polyprenol-phosphate-mannose (PPM) to a monoacyl phosphatidylinositol pentamannoside (AcPIM5) to generate a monoacyl phosphatidylinositol hexamannoside (AcPIM6) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PIGM family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018584 [H]

Pfam domain/function: PF09594 DUF2029 [H]

EC number: NA

Molecular weight: Translated: 46381; Mature: 46250

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLPLPIPRSDGAKAAPTVGRYSRPMRAASALKVRPCLARVVALAPILLAVSIIARLAS
CCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMVYYFFAGDAFFDLHVYVIGGAALNHPGTLYDVVYVDPTKGERLPFTYPAFAAMVFYPL
HHHHHHHCCCCEEEEEEEEEECHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
HLLPFGLLAFLWQIALIAAIYASIRISQRLIGDGAGGPRVAMLWTAVAIWLEPVASSVQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GQVGVFLMLAVLYAAYSSRWWLSGLLVGAGAGVKLTPAITGLYFVGMRRWAAAAFSAVTF
CCHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
FATVGLAYVALPADAGRYFPDQMTEAGTTLPIGSSWNQSWLGGLSRILGYDPGLHPLVIA
HHHHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
AVAVTALLAALAWRALGSADGRDRLASLLVVQFLGLMASPVSWIHHWVWVIPLIIWLIHG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PWRERAGARLLGLGWLALMVISVPSLLSLAQPDIWEISRPWYLAWAEMVYIVASLSTLAW
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IIRTGRRA
HHHCCCCC
>Mature Secondary Structure 
TRLPLPIPRSDGAKAAPTVGRYSRPMRAASALKVRPCLARVVALAPILLAVSIIARLAS
CCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMVYYFFAGDAFFDLHVYVIGGAALNHPGTLYDVVYVDPTKGERLPFTYPAFAAMVFYPL
HHHHHHHCCCCEEEEEEEEEECHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
HLLPFGLLAFLWQIALIAAIYASIRISQRLIGDGAGGPRVAMLWTAVAIWLEPVASSVQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GQVGVFLMLAVLYAAYSSRWWLSGLLVGAGAGVKLTPAITGLYFVGMRRWAAAAFSAVTF
CCHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
FATVGLAYVALPADAGRYFPDQMTEAGTTLPIGSSWNQSWLGGLSRILGYDPGLHPLVIA
HHHHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
AVAVTALLAALAWRALGSADGRDRLASLLVVQFLGLMASPVSWIHHWVWVIPLIIWLIHG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PWRERAGARLLGLGWLALMVISVPSLLSLAQPDIWEISRPWYLAWAEMVYIVASLSTLAW
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IIRTGRRA
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]