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The map label for this gene is nirD [H]

Identifier: 108797239

GI number: 108797239

Start: 300359

End: 300730

Strand: Direct

Name: nirD [H]

Synonym: Mmcs_0259

Alternate gene names: 108797239

Gene position: 300359-300730 (Clockwise)

Preceding gene: 108797238

Following gene: 108797241

Centisome position: 5.26

GC content: 70.7

Gene sequence:

>372_bases
GTGACCCTGCTCGACGACCGCAACGAGCTCAACGGGCCGCCGGATACGCGTTGGACGGTGGCCTGCCGCTACGACTACCT
CATCCCGTGCCGCGGCGTCGGGGTGCTCTTGCCCGACGGTGCCCAGGCGGCGCTGTTCCGTCTCGACGACGGGAGCCTGC
ACGCGATCGGCAACATCGACCCCTTCTCCGGGGCGGCGGTGATGTCGCGCGGTATCACCGGCGACCGGGGCGGGCGGGCC
ATCGTGCAGTCCCCGATCAAGAAGCAGGCCTTCGCCTTCGACGACGGCAGCTGCCTCGACGACCCGTCGGTGTCACTGCC
GGTGTACCGGACGCGGCGGACCGCCGACGGGATCGTCGAGATCGAGGCCTAG

Upstream 100 bases:

>100_bases
CCGATGTGCCCGACCCGACCGTCACCTTCACCGAGAACGCCGGCCGCAAGGTGCCGGTGCCCATCGGGATGCCATCCATC
CGACCGCTTCAGGAGACACC

Downstream 100 bases:

>100_bases
CGTTCAGGCTGCGCGCCGGTACTCCACCGGGCTGCGGTGGGTGAGGGCCCGCACCGGTTGCCAGCGGAACACGCCCGCAC
CCTGCGCATAGGACAGGTGG

Product: nitrite reductase (NAD(P)H) large subunit, NirD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MTLLDDRNELNGPPDTRWTVACRYDYLIPCRGVGVLLPDGAQAALFRLDDGSLHAIGNIDPFSGAAVMSRGITGDRGGRA
IVQSPIKKQAFAFDDGSCLDDPSVSLPVYRTRRTADGIVEIEA

Sequences:

>Translated_123_residues
MTLLDDRNELNGPPDTRWTVACRYDYLIPCRGVGVLLPDGAQAALFRLDDGSLHAIGNIDPFSGAAVMSRGITGDRGGRA
IVQSPIKKQAFAFDDGSCLDDPSVSLPVYRTRRTADGIVEIEA
>Mature_122_residues
TLLDDRNELNGPPDTRWTVACRYDYLIPCRGVGVLLPDGAQAALFRLDDGSLHAIGNIDPFSGAAVMSRGITGDRGGRAI
VQSPIKKQAFAFDDGSCLDDPSVSLPVYRTRRTADGIVEIEA

Specific function: Required for activity of the reductase [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nasE [H]

Homologues:

Organism=Escherichia coli, GI1789766, Length=107, Percent_Identity=39.2523364485981, Blast_Score=79, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR017941 [H]

Pfam domain/function: NA

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 13222; Mature: 13091

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLDDRNELNGPPDTRWTVACRYDYLIPCRGVGVLLPDGAQAALFRLDDGSLHAIGNID
CCCCCCCCCCCCCCCCCEEEEEEECEEECCCCCEEEECCCCCEEEEEECCCCEEEECCCC
PFSGAAVMSRGITGDRGGRAIVQSPIKKQAFAFDDGSCLDDPSVSLPVYRTRRTADGIVE
CCCCHHHHHCCCCCCCCCCCEECCCCCCCEEECCCCCCCCCCCCCCCEEECCCCCCCEEE
IEA
EEC
>Mature Secondary Structure 
TLLDDRNELNGPPDTRWTVACRYDYLIPCRGVGVLLPDGAQAALFRLDDGSLHAIGNID
CCCCCCCCCCCCCCCCEEEEEEECEEECCCCCEEEECCCCCEEEEEECCCCEEEECCCC
PFSGAAVMSRGITGDRGGRAIVQSPIKKQAFAFDDGSCLDDPSVSLPVYRTRRTADGIVE
CCCCHHHHHCCCCCCCCCCCEECCCCCCCEEECCCCCCCCCCCCCCCEEECCCCCCCEEE
IEA
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]