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The map label for this gene is puuP [H]

Identifier: 108797212

GI number: 108797212

Start: 258005

End: 259540

Strand: Reverse

Name: puuP [H]

Synonym: Mmcs_0232

Alternate gene names: 108797212

Gene position: 259540-258005 (Counterclockwise)

Preceding gene: 108797213

Following gene: 108797211

Centisome position: 4.55

GC content: 68.23

Gene sequence:

>1536_bases
TTGACCACCGCCGAGAGGAAACCCCCGATGACCCAGACGCCGCCCGAGACCAACTTGGCACCCGAAACCGACCAGCTGCC
GCACCGCCGCCTGCCGTTCTGGGTGGCACTGGCCATGTCGGTGGCGCTCGTCGGTCCGACGCTGGCCATGTCCGGTAACG
GACAGGGCCTCATCGGCACCGTGGGCAAGGCCATCCCGCTGGTCTTCCTGATCGGTCTGATCGGCGTCTCCCTGGTCGGG
TACAGCTTCGTGCGGCTGACCCGCCACCTCAACCACGCCGGGTCGGCATACGGTCTGGTCGGCGGCACCATCGGACCGCG
CGCGGGCTTCTTCTCCGGCTTCGCGATGCTCGGTGCCTACGTCGGCTTCTCGATCGGCACACTGGCGCTGACCGCCGCGT
TCACCAACGCGTTCATCGCGCAACTGCAACCCGACGCCGAAGACCCCTACCAGGTGCCGTGGCTGCTGGTGGTGCTGGTG
GGTGCGGGGATCTCGTTCCTGTTGGCGGGCCGCGACATCCGGCTGCTGGCGAAGATCCTGCTCGGCATCGAAGGCATCGG
CATCCTCGCCATGGTGATACTGGTCGCCGTCATCTTCGCCAAGGGCGGGGCGCCGACGACGGGCGTCGACTTCAGCGTGT
TCACCTTCGACGGGGTCTCCGCGTCCGCGGTGCTCAGCGGCGTGGTGGCCGCCTTCCTGTCCTGGGCCGGTTTCGAGGCC
TGCGCCTCGATGGGCGAGGAGACCGACGATCCCCGCCGCAACATCCCCCGCGCCCTCGGCGGCACCCTGCTGCTCACCGG
TGTGCTATTCGTCGTCGTGATGTTCGCCCAGGTCATCGGGTTCGGCACCGACGAGGCCGGCCTGGCCGCCTTCCAGGGCT
CCGGGAACACGCTGGGCGACTTGGGCAGCACCTATGTCGGACAGTGGTTCTCGCTGTTGATCATCTTCACCGCGACGGTC
GCGGCGTTCGGCTGCCACCTGGCCACCTCGGCCACCTCCGGTCGCATGCTCTACGCGTTCGGCCGCGACGGGTTCGGTCC
GAAAGCGTTGGCGCACATCCACGCCGAGACCGGTGGCCCGCGGCGGGCCACCTGGCTGGTCGTCGGCGTGGCACTGGTCG
TCGACCTGATCTGCGGTGCGCTCGACTGGCCCGACATGGGCACCGGCAACGACGCGATCGACACCTACTTCCTGTTCGCC
GTCGCGGGTTCGGTGTGCCTGATGGTGTGCTACCTGCTGGTCGAGATCGCCGCGGCCTGGTTCGTCGGCGCACCGAAGTT
CGTCGGTGTGCACGGCGGGACGGGCAAGGTGCTCGGTCTGCTGCTGCCACTGCTCGGCGCGGTGGTGATCGCGGTGGTGT
TGTGGTTCAACGTCAAGGACGCCGAGACGTGGTCGGCCGCACCGCTTCTCGGATTGTACTGGTGCGCAATCGGTTTGATC
ATCGCGCTGGCAGCGTCCGGAATCGCCAAACGGGTCGGTGAGTCGCTGAGCCGTGAGCTCGAGCTCAGCCCGCCGGACGG
CCGACCGGTCAGCTGA

Upstream 100 bases:

>100_bases
TCATCGAGGCGCTGATCGCCGTCCGGCGCCACGAGCTGACCACCTACGGCGACCGTCCGCCCGCCGAGACCACCCAGGCG
CTGCGCCTCGCCTGGAGCTG

Downstream 100 bases:

>100_bases
GAGCCGCCGGTGGGCTACGTTGGCGGCGACGGGGCCGCAGGGAAGGTCGACGACGAGGAGGTCGCGAAGTATGCCGGTTG
GTGATCGCACCGATGTGTTC

Product: amino acid permease-associated protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTTAERKPPMTQTPPETNLAPETDQLPHRRLPFWVALAMSVALVGPTLAMSGNGQGLIGTVGKAIPLVFLIGLIGVSLVG
YSFVRLTRHLNHAGSAYGLVGGTIGPRAGFFSGFAMLGAYVGFSIGTLALTAAFTNAFIAQLQPDAEDPYQVPWLLVVLV
GAGISFLLAGRDIRLLAKILLGIEGIGILAMVILVAVIFAKGGAPTTGVDFSVFTFDGVSASAVLSGVVAAFLSWAGFEA
CASMGEETDDPRRNIPRALGGTLLLTGVLFVVVMFAQVIGFGTDEAGLAAFQGSGNTLGDLGSTYVGQWFSLLIIFTATV
AAFGCHLATSATSGRMLYAFGRDGFGPKALAHIHAETGGPRRATWLVVGVALVVDLICGALDWPDMGTGNDAIDTYFLFA
VAGSVCLMVCYLLVEIAAAWFVGAPKFVGVHGGTGKVLGLLLPLLGAVVIAVVLWFNVKDAETWSAAPLLGLYWCAIGLI
IALAASGIAKRVGESLSRELELSPPDGRPVS

Sequences:

>Translated_511_residues
MTTAERKPPMTQTPPETNLAPETDQLPHRRLPFWVALAMSVALVGPTLAMSGNGQGLIGTVGKAIPLVFLIGLIGVSLVG
YSFVRLTRHLNHAGSAYGLVGGTIGPRAGFFSGFAMLGAYVGFSIGTLALTAAFTNAFIAQLQPDAEDPYQVPWLLVVLV
GAGISFLLAGRDIRLLAKILLGIEGIGILAMVILVAVIFAKGGAPTTGVDFSVFTFDGVSASAVLSGVVAAFLSWAGFEA
CASMGEETDDPRRNIPRALGGTLLLTGVLFVVVMFAQVIGFGTDEAGLAAFQGSGNTLGDLGSTYVGQWFSLLIIFTATV
AAFGCHLATSATSGRMLYAFGRDGFGPKALAHIHAETGGPRRATWLVVGVALVVDLICGALDWPDMGTGNDAIDTYFLFA
VAGSVCLMVCYLLVEIAAAWFVGAPKFVGVHGGTGKVLGLLLPLLGAVVIAVVLWFNVKDAETWSAAPLLGLYWCAIGLI
IALAASGIAKRVGESLSRELELSPPDGRPVS
>Mature_510_residues
TTAERKPPMTQTPPETNLAPETDQLPHRRLPFWVALAMSVALVGPTLAMSGNGQGLIGTVGKAIPLVFLIGLIGVSLVGY
SFVRLTRHLNHAGSAYGLVGGTIGPRAGFFSGFAMLGAYVGFSIGTLALTAAFTNAFIAQLQPDAEDPYQVPWLLVVLVG
AGISFLLAGRDIRLLAKILLGIEGIGILAMVILVAVIFAKGGAPTTGVDFSVFTFDGVSASAVLSGVVAAFLSWAGFEAC
ASMGEETDDPRRNIPRALGGTLLLTGVLFVVVMFAQVIGFGTDEAGLAAFQGSGNTLGDLGSTYVGQWFSLLIIFTATVA
AFGCHLATSATSGRMLYAFGRDGFGPKALAHIHAETGGPRRATWLVVGVALVVDLICGALDWPDMGTGNDAIDTYFLFAV
AGSVCLMVCYLLVEIAAAWFVGAPKFVGVHGGTGKVLGLLLPLLGAVVIAVVLWFNVKDAETWSAAPLLGLYWCAIGLII
ALAASGIAKRVGESLSRELELSPPDGRPVS

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081869, Length=469, Percent_Identity=25.1599147121535, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082023, Length=475, Percent_Identity=23.7894736842105, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 52974; Mature: 52842

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAERKPPMTQTPPETNLAPETDQLPHRRLPFWVALAMSVALVGPTLAMSGNGQGLIGT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHH
VGKAIPLVFLIGLIGVSLVGYSFVRLTRHLNHAGSAYGLVGGTIGPRAGFFSGFAMLGAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHH
VGFSIGTLALTAAFTNAFIAQLQPDAEDPYQVPWLLVVLVGAGISFLLAGRDIRLLAKIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
LGIEGIGILAMVILVAVIFAKGGAPTTGVDFSVFTFDGVSASAVLSGVVAAFLSWAGFEA
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
CASMGEETDDPRRNIPRALGGTLLLTGVLFVVVMFAQVIGFGTDEAGLAAFQGSGNTLGD
HHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHH
LGSTYVGQWFSLLIIFTATVAAFGCHLATSATSGRMLYAFGRDGFGPKALAHIHAETGGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHEEECCCCCC
RRATWLVVGVALVVDLICGALDWPDMGTGNDAIDTYFLFAVAGSVCLMVCYLLVEIAAAW
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVGAPKFVGVHGGTGKVLGLLLPLLGAVVIAVVLWFNVKDAETWSAAPLLGLYWCAIGLI
HCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
IALAASGIAKRVGESLSRELELSPPDGRPVS
HHHHHHHHHHHHHHHHHCCEECCCCCCCCCC
>Mature Secondary Structure 
TTAERKPPMTQTPPETNLAPETDQLPHRRLPFWVALAMSVALVGPTLAMSGNGQGLIGT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHH
VGKAIPLVFLIGLIGVSLVGYSFVRLTRHLNHAGSAYGLVGGTIGPRAGFFSGFAMLGAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHH
VGFSIGTLALTAAFTNAFIAQLQPDAEDPYQVPWLLVVLVGAGISFLLAGRDIRLLAKIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
LGIEGIGILAMVILVAVIFAKGGAPTTGVDFSVFTFDGVSASAVLSGVVAAFLSWAGFEA
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
CASMGEETDDPRRNIPRALGGTLLLTGVLFVVVMFAQVIGFGTDEAGLAAFQGSGNTLGD
HHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHH
LGSTYVGQWFSLLIIFTATVAAFGCHLATSATSGRMLYAFGRDGFGPKALAHIHAETGGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHEEECCCCCC
RRATWLVVGVALVVDLICGALDWPDMGTGNDAIDTYFLFAVAGSVCLMVCYLLVEIAAAW
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVGAPKFVGVHGGTGKVLGLLLPLLGAVVIAVVLWFNVKDAETWSAAPLLGLYWCAIGLI
HCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
IALAASGIAKRVGESLSRELELSPPDGRPVS
HHHHHHHHHHHHHHHHHCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]