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The map label for this gene is baiB [H]

Identifier: 108797203

GI number: 108797203

Start: 244949

End: 246424

Strand: Reverse

Name: baiB [H]

Synonym: Mmcs_0223

Alternate gene names: 108797203

Gene position: 246424-244949 (Counterclockwise)

Preceding gene: 108797204

Following gene: 108797201

Centisome position: 4.32

GC content: 70.46

Gene sequence:

>1476_bases
ATGGTCGACGGCGTACCCCCGATCGGGACGCAGTTCTCGGCCCTGGCCGCCCGGGATCCCGACGCACCGGCGCTGACGTG
CGCCGGGCGCACCGTCACCCGTGGTGAACTCGACGTGACGACCAACCGGCTGGCCCGCGCGTACGCCGAACTCGGTGTGC
GCCAAGGTGATTACGTCACCATACTGCTGCCCAACTCGATCGCCTGGGTGGAGGCCGTGCTGGCGACGTGGAAACTGGGC
GCGGTGCCGCAGCCGCTGTCTGCCCGGCTCCCGGATGCCGAACTGTCCGCACTGCTGGCGCTGCGCCCACCCGCCCTGCT
CGTCGGGCGCGCCGACCCCAACGACGTATCTCCCTCTGTCCCCGCGAATTTCACGCCCGATCCGGCGTTGCCGGCCGACC
CGCTTCCGGAGGCCGTCTCACCGGCGTGGAAGTCGATGGCCTCCGGCGGCAGCACCGGCAGACCGAAGCTCATCGAGGCC
GGCGGTGACAGCCGGGTGCCGTCGGCGATCGGTCTGCCCCTGGGCGCCGAGGAGGGTGACGTCAACCTGGTGTCGGTGCC
GTTGAGCCACAACACGGGGTTCACCACGCTCGCGATCGGGCTGCTGATGGGCCATCACCTCGTGCTGATGCCGCGGTTCG
AACCACACGAGTTCCTGCGCCTGGTGAGCGAACACCGGGTGACCTTCCTGACCACCGTGCCGACGATCATGCAGCGACTG
CTGCCGGTGTACCGGGCCGACCCCGGTGCCTACGACCTGTCGTCGATCCGGCGGTTCTGGCATGTCGGCGCGCCCTGCCC
GCCCGCGGTCAAGGAGGCCTGGATCGGGCTGCTGGGACCGCAGGTTCTCTGGGAACTGTACGGCGGCACCGAGTTACAGG
CGCTGACGTTCATCTCCGGCGAACAGTGGCTGACCCACCGCGGGTCGGTCGGCACGGTGGTGGCCGGGGCGATGACGGTG
CTCGACGACGACGGCAACACCTGCCCGCCCGGCGTCGTCGGCGAGATCTACATGCGCCGCTCCCCGGGCGCCGCCCCCAC
CTACCGCTACATCGGCAGCACCGCCAAGAGCCGCGACGGGTGGGATTCGCTCGGCGATCTGGGCTACTTCGACGAGGACG
GTTTCCTCTACCTCAACGACCGGCGCGTCGACATGTTCACCGTCGGCGGCCGCAACGTCTATCCCGCCGAGATCGAGTCG
GCGCTCTCGGCGCATCCGGACGTCCTGTCGTGCCTCGTCGTCGGCGTCCCGGACGACGACCTCGGTCAGGTGCCCCACGC
ACTCGTGCAGCGGGCACCCGGATCGGCGCTCGACGAGGCGGCGGTCACGGCGTTCGTGGCCGAGCGCCTGGCCGGCTACA
AGGTGCCGCGCACCGTCGAGTTCAGCGAACATCCGCTGCGCGACGACGCGGGTAAGGCCCGTCGCTCCGCCGTCCGCGCC
GAGGTCATCGCGAGATCGGCTCGGGCACGTCCGTGA

Upstream 100 bases:

>100_bases
CTGCCCGCGCTGCGCGCCCGTGGCGTCACCGACGAACAGCTCACCACGATGCTCGTCGACAATCCCCGGCGGATCTTCGA
GCACTCGGGCGGGTACTGAG

Downstream 100 bases:

>100_bases
CCGAACTGTCCAGCGGCGGCGCCCTGTGCGTCGGCGTGGCCCGTCGGAACTCCCAGCGGCGCAGCGCCTCCCGGGCCGGG
CTCTCCACGCACGCGTAGCT

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MVDGVPPIGTQFSALAARDPDAPALTCAGRTVTRGELDVTTNRLARAYAELGVRQGDYVTILLPNSIAWVEAVLATWKLG
AVPQPLSARLPDAELSALLALRPPALLVGRADPNDVSPSVPANFTPDPALPADPLPEAVSPAWKSMASGGSTGRPKLIEA
GGDSRVPSAIGLPLGAEEGDVNLVSVPLSHNTGFTTLAIGLLMGHHLVLMPRFEPHEFLRLVSEHRVTFLTTVPTIMQRL
LPVYRADPGAYDLSSIRRFWHVGAPCPPAVKEAWIGLLGPQVLWELYGGTELQALTFISGEQWLTHRGSVGTVVAGAMTV
LDDDGNTCPPGVVGEIYMRRSPGAAPTYRYIGSTAKSRDGWDSLGDLGYFDEDGFLYLNDRRVDMFTVGGRNVYPAEIES
ALSAHPDVLSCLVVGVPDDDLGQVPHALVQRAPGSALDEAAVTAFVAERLAGYKVPRTVEFSEHPLRDDAGKARRSAVRA
EVIARSARARP

Sequences:

>Translated_491_residues
MVDGVPPIGTQFSALAARDPDAPALTCAGRTVTRGELDVTTNRLARAYAELGVRQGDYVTILLPNSIAWVEAVLATWKLG
AVPQPLSARLPDAELSALLALRPPALLVGRADPNDVSPSVPANFTPDPALPADPLPEAVSPAWKSMASGGSTGRPKLIEA
GGDSRVPSAIGLPLGAEEGDVNLVSVPLSHNTGFTTLAIGLLMGHHLVLMPRFEPHEFLRLVSEHRVTFLTTVPTIMQRL
LPVYRADPGAYDLSSIRRFWHVGAPCPPAVKEAWIGLLGPQVLWELYGGTELQALTFISGEQWLTHRGSVGTVVAGAMTV
LDDDGNTCPPGVVGEIYMRRSPGAAPTYRYIGSTAKSRDGWDSLGDLGYFDEDGFLYLNDRRVDMFTVGGRNVYPAEIES
ALSAHPDVLSCLVVGVPDDDLGQVPHALVQRAPGSALDEAAVTAFVAERLAGYKVPRTVEFSEHPLRDDAGKARRSAVRA
EVIARSARARP
>Mature_491_residues
MVDGVPPIGTQFSALAARDPDAPALTCAGRTVTRGELDVTTNRLARAYAELGVRQGDYVTILLPNSIAWVEAVLATWKLG
AVPQPLSARLPDAELSALLALRPPALLVGRADPNDVSPSVPANFTPDPALPADPLPEAVSPAWKSMASGGSTGRPKLIEA
GGDSRVPSAIGLPLGAEEGDVNLVSVPLSHNTGFTTLAIGLLMGHHLVLMPRFEPHEFLRLVSEHRVTFLTTVPTIMQRL
LPVYRADPGAYDLSSIRRFWHVGAPCPPAVKEAWIGLLGPQVLWELYGGTELQALTFISGEQWLTHRGSVGTVVAGAMTV
LDDDGNTCPPGVVGEIYMRRSPGAAPTYRYIGSTAKSRDGWDSLGDLGYFDEDGFLYLNDRRVDMFTVGGRNVYPAEIES
ALSAHPDVLSCLVVGVPDDDLGQVPHALVQRAPGSALDEAAVTAFVAERLAGYKVPRTVEFSEHPLRDDAGKARRSAVRA
EVIARSARARP

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=543, Percent_Identity=25.414364640884, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI42544132, Length=525, Percent_Identity=24.952380952381, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI187761345, Length=514, Percent_Identity=28.2101167315175, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI187761343, Length=514, Percent_Identity=28.2101167315175, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI157311624, Length=485, Percent_Identity=24.7422680412371, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI157311622, Length=485, Percent_Identity=24.7422680412371, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI38505220, Length=483, Percent_Identity=26.0869565217391, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI58082049, Length=485, Percent_Identity=24.7422680412371, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI122937307, Length=481, Percent_Identity=26.4033264033264, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI115511026, Length=496, Percent_Identity=23.991935483871, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI13375727, Length=532, Percent_Identity=24.0601503759398, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1788107, Length=533, Percent_Identity=27.9549718574109, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI221142682, Length=505, Percent_Identity=27.5247524752475, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693145, Length=500, Percent_Identity=27.6, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1786801, Length=485, Percent_Identity=26.5979381443299, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790505, Length=529, Percent_Identity=24.7637051039698, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786810, Length=505, Percent_Identity=23.3663366336634, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559526, Length=305, Percent_Identity=32.1311475409836, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=530, Percent_Identity=24.7169811320755, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=482, Percent_Identity=25.103734439834, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=487, Percent_Identity=25.6673511293634, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=527, Percent_Identity=24.6679316888046, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=543, Percent_Identity=23.3885819521179, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=543, Percent_Identity=23.3885819521179, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=545, Percent_Identity=23.6697247706422, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=524, Percent_Identity=23.8549618320611, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=356, Percent_Identity=29.2134831460674, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=290, Percent_Identity=26.2068965517241, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=348, Percent_Identity=27.0114942528736, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=482, Percent_Identity=24.2738589211618, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983001, Length=496, Percent_Identity=24.1935483870968, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=323, Percent_Identity=26.9349845201238, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323182, Length=255, Percent_Identity=23.1372549019608, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648253, Length=468, Percent_Identity=26.9230769230769, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24648255, Length=509, Percent_Identity=26.5225933202358, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21356441, Length=490, Percent_Identity=25.9183673469388, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355181, Length=497, Percent_Identity=26.9617706237425, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21356947, Length=489, Percent_Identity=25.5623721881391, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI281365686, Length=489, Percent_Identity=23.1083844580777, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21358303, Length=488, Percent_Identity=23.9754098360656, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24653035, Length=486, Percent_Identity=24.0740740740741, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648257, Length=435, Percent_Identity=28.735632183908, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI18859661, Length=455, Percent_Identity=23.5164835164835, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161076582, Length=499, Percent_Identity=23.2464929859719, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24581924, Length=516, Percent_Identity=24.2248062015504, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24656500, Length=493, Percent_Identity=25.1521298174442, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648260, Length=493, Percent_Identity=23.9350912778905, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922652, Length=432, Percent_Identity=26.1574074074074, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI20130357, Length=348, Percent_Identity=28.735632183908, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI21355225, Length=480, Percent_Identity=24.375, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI62472339, Length=544, Percent_Identity=22.7941176470588, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24667955, Length=545, Percent_Identity=23.1192660550459, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24667959, Length=375, Percent_Identity=24.2666666666667, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 52354; Mature: 52354

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDGVPPIGTQFSALAARDPDAPALTCAGRTVTRGELDVTTNRLARAYAELGVRQGDYVT
CCCCCCCCCCCHHHHHCCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEE
ILLPNSIAWVEAVLATWKLGAVPQPLSARLPDAELSALLALRPPALLVGRADPNDVSPSV
EECCCCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCC
PANFTPDPALPADPLPEAVSPAWKSMASGGSTGRPKLIEAGGDSRVPSAIGLPLGAEEGD
CCCCCCCCCCCCCCCCHHHCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCC
VNLVSVPLSHNTGFTTLAIGLLMGHHLVLMPRFEPHEFLRLVSEHRVTFLTTVPTIMQRL
EEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEEEHHHHHHHHHH
LPVYRADPGAYDLSSIRRFWHVGAPCPPAVKEAWIGLLGPQVLWELYGGTELQALTFISG
HHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHCCCCCEEEEEEECC
EQWLTHRGSVGTVVAGAMTVLDDDGNTCPPGVVGEIYMRRSPGAAPTYRYIGSTAKSRDG
CHHHHCCCCCHHHHHCEEEEEECCCCCCCCCCHHHHEEECCCCCCCHHHHHCCCCCCCCC
WDSLGDLGYFDEDGFLYLNDRRVDMFTVGGRNVYPAEIESALSAHPDVLSCLVVGVPDDD
CHHHCCCCCCCCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCH
LGQVPHALVQRAPGSALDEAAVTAFVAERLAGYKVPRTVEFSEHPLRDDAGKARRSAVRA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHH
EVIARSARARP
HHHHHHHCCCC
>Mature Secondary Structure
MVDGVPPIGTQFSALAARDPDAPALTCAGRTVTRGELDVTTNRLARAYAELGVRQGDYVT
CCCCCCCCCCCHHHHHCCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEE
ILLPNSIAWVEAVLATWKLGAVPQPLSARLPDAELSALLALRPPALLVGRADPNDVSPSV
EECCCCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCC
PANFTPDPALPADPLPEAVSPAWKSMASGGSTGRPKLIEAGGDSRVPSAIGLPLGAEEGD
CCCCCCCCCCCCCCCCHHHCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCC
VNLVSVPLSHNTGFTTLAIGLLMGHHLVLMPRFEPHEFLRLVSEHRVTFLTTVPTIMQRL
EEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEEEHHHHHHHHHH
LPVYRADPGAYDLSSIRRFWHVGAPCPPAVKEAWIGLLGPQVLWELYGGTELQALTFISG
HHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHCCCCCEEEEEEECC
EQWLTHRGSVGTVVAGAMTVLDDDGNTCPPGVVGEIYMRRSPGAAPTYRYIGSTAKSRDG
CHHHHCCCCCHHHHHCEEEEEECCCCCCCCCCHHHHEEECCCCCCCHHHHHCCCCCCCCC
WDSLGDLGYFDEDGFLYLNDRRVDMFTVGGRNVYPAEIESALSAHPDVLSCLVVGVPDDD
CHHHCCCCCCCCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCH
LGQVPHALVQRAPGSALDEAAVTAFVAERLAGYKVPRTVEFSEHPLRDDAGKARRSAVRA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHH
EVIARSARARP
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]