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The map label for this gene is ptsI [H]

Identifier: 108797064

GI number: 108797064

Start: 96454

End: 98208

Strand: Direct

Name: ptsI [H]

Synonym: Mmcs_0083

Alternate gene names: 108797064

Gene position: 96454-98208 (Clockwise)

Preceding gene: 108797055

Following gene: 108797065

Centisome position: 1.69

GC content: 74.25

Gene sequence:

>1755_bases
ATGTTTGGTTTTACCCCCGTTCGTGTTGACTTGTCAACACTTTTTAGTAACCTGGTTCACATGACCGCTTCATCTGCGCC
CACATCACACGCCACTGCCGGCACGGTGCTGCGCGGCGTGCCCGTCGTTCCCGGGGTGCAGTACGCCCCGGTGGTCCGGC
CCGGCCGGCTGCCGGTCGTCGAGGACACCGCCGCCGAGATCGCGGACGCCGACCGACCCGCCGAGGCCGCCCGCTTCAGC
GCCGCGGCGACCGCCGTCGCCACCCGCCTGCGCGACCGCGCCGCGCACTCGACCGGGGCGGCCTCCGAGGTGCTCGCCGC
CACCGCGACCCTTGCCCAGGACCGGGCCTGGTTGGGCGCCGCGGAGAAACGCATCGCCGGCGGTACCCCGGCCGTCCGCG
CGGTGCGGGAAGCCGTCGCCCAGTTCGTCGACCTGTTCACGAAGATGGGCGGGCTGATGGCGGAGCGGGTCACCGACCTG
CAGGACATCCGCGACCGCGTGCTGGCCGAGCTCAGCGGGCTGCCCGAACCCGGTGTCCCGCTGCCGATGGTCCCGTCGGT
CCTGTGCGCCGAGGACCTCGCCCCCGCCGACACCGCGGGTCTCGATCCCACGCTCATCGTCGCGCTGGCGACCACACTGG
GTGGCCCGACCAGTCACACCGCGATCATCGCCCGTCAGCTCGGCATCCCGTGCGTGGTCGCCGTCGACGGCCTCGACGCG
GTACCCGCCGGCGCGTTCGTGCTGGTCGACGGCACGGCAGGCACGGTGACCGTCGGCCCCGACGAGGTGGCCGCTGCCGA
GGCCGTCGCCGCGGCCCGGCACGAGGCCGAGCGCGCCGCGCACTGGGCCGGGCCCGGGGCGACCGCCGACGGCCATGCCG
TCGCGATCCTGGCCAACGTGCAGGACGGTGCCGCGGCGCGGGCGGCCCGCGAGACGCCGGCCGAAGGCGTCGGCCTGTTC
CGCACCGAACTGTGCTTCCTCAACCGCGACACCGAACCGACCGTCGACGAGCAGGCCGAGATCTACGGTGAGGTGCTCGA
CGCCTTCGCGGGGCACAAGGTCGTGGTCCGCACGCTCGACGCCGGTTCGGACAAACCGCTCAAGTTCGCCGGACACCGCG
ACGAGGCCAACCCGGCACTGGGTGTGCGGGGCATCCGGATCGCCCACGGCAACCCGGGCATCCTCGAACGGCAACTCGCC
GGTATCGCGGCCGCGGCGGCGCGCACCGGCACCCGACCGTGGGTGATGGCGCCGATGATCGCGACCGCCGGGGAGGCGAA
GGACTTCGCCGCCGCCGCGCGCCGCCACGGGCTGACGCCGGGGGTGATGATCGAGGTGCCCGCCGCCGCCCTGCTGGCCG
ACCGCATCCTCGAACACGTCGACTTCCTGTCGATCGGCACCAACGACCTCGCGCAGTACACGATGGCCGCCGACCGGATG
TCGGCCGACCTGGCATTGCTCACCGACCCGTGGCAGCCGGCGGTACTCGCGCTCGTCGCGATGACCGTGCGGGCCGGCGC
CGCGGCGGGCAAACCCGTCGGCGTGTGCGGTGAGGCGGCGGCCGACCCGACGCTCGCCTGCGTCTTGGCGGGTCTCGGCG
TGACGTCGCTGTCCGCCGCGTCCGCGGCGGTTCCCGGAGTGGGCGCCAGGCTGGCCCAGGTCACCCTGCAGCAGTGCCGC
GATGCGGCCGAGGCGGTGCTGGCCACGGCGACCGCCGCCGAAGCGCGCGCCGCCGCGCTCGCCGCCCTCGGGTGA

Upstream 100 bases:

>100_bases
CGCGGCCCTTCTGCATCACCAGGGAGGCGATGGCCTGCTGCCGTTCTTCGGCGTACATGGAGCTCCGTTTATGTGGGAAG
TGGCGGGAAGGTGCTTGGTT

Downstream 100 bases:

>100_bases
CGGAATGAATCGGACCGCAGTCCGGTTGATCGGAACATGCCTGCCATCACCGCCGACACCCTGACCCTGCCCCGCGTGAC
GGCGCCCGCGCCGTCCGACA

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MFGFTPVRVDLSTLFSNLVHMTASSAPTSHATAGTVLRGVPVVPGVQYAPVVRPGRLPVVEDTAAEIADADRPAEAARFS
AAATAVATRLRDRAAHSTGAASEVLAATATLAQDRAWLGAAEKRIAGGTPAVRAVREAVAQFVDLFTKMGGLMAERVTDL
QDIRDRVLAELSGLPEPGVPLPMVPSVLCAEDLAPADTAGLDPTLIVALATTLGGPTSHTAIIARQLGIPCVVAVDGLDA
VPAGAFVLVDGTAGTVTVGPDEVAAAEAVAAARHEAERAAHWAGPGATADGHAVAILANVQDGAAARAARETPAEGVGLF
RTELCFLNRDTEPTVDEQAEIYGEVLDAFAGHKVVVRTLDAGSDKPLKFAGHRDEANPALGVRGIRIAHGNPGILERQLA
GIAAAAARTGTRPWVMAPMIATAGEAKDFAAAARRHGLTPGVMIEVPAAALLADRILEHVDFLSIGTNDLAQYTMAADRM
SADLALLTDPWQPAVLALVAMTVRAGAAAGKPVGVCGEAAADPTLACVLAGLGVTSLSAASAAVPGVGARLAQVTLQQCR
DAAEAVLATATAAEARAAALAALG

Sequences:

>Translated_584_residues
MFGFTPVRVDLSTLFSNLVHMTASSAPTSHATAGTVLRGVPVVPGVQYAPVVRPGRLPVVEDTAAEIADADRPAEAARFS
AAATAVATRLRDRAAHSTGAASEVLAATATLAQDRAWLGAAEKRIAGGTPAVRAVREAVAQFVDLFTKMGGLMAERVTDL
QDIRDRVLAELSGLPEPGVPLPMVPSVLCAEDLAPADTAGLDPTLIVALATTLGGPTSHTAIIARQLGIPCVVAVDGLDA
VPAGAFVLVDGTAGTVTVGPDEVAAAEAVAAARHEAERAAHWAGPGATADGHAVAILANVQDGAAARAARETPAEGVGLF
RTELCFLNRDTEPTVDEQAEIYGEVLDAFAGHKVVVRTLDAGSDKPLKFAGHRDEANPALGVRGIRIAHGNPGILERQLA
GIAAAAARTGTRPWVMAPMIATAGEAKDFAAAARRHGLTPGVMIEVPAAALLADRILEHVDFLSIGTNDLAQYTMAADRM
SADLALLTDPWQPAVLALVAMTVRAGAAAGKPVGVCGEAAADPTLACVLAGLGVTSLSAASAAVPGVGARLAQVTLQQCR
DAAEAVLATATAAEARAAALAALG
>Mature_584_residues
MFGFTPVRVDLSTLFSNLVHMTASSAPTSHATAGTVLRGVPVVPGVQYAPVVRPGRLPVVEDTAAEIADADRPAEAARFS
AAATAVATRLRDRAAHSTGAASEVLAATATLAQDRAWLGAAEKRIAGGTPAVRAVREAVAQFVDLFTKMGGLMAERVTDL
QDIRDRVLAELSGLPEPGVPLPMVPSVLCAEDLAPADTAGLDPTLIVALATTLGGPTSHTAIIARQLGIPCVVAVDGLDA
VPAGAFVLVDGTAGTVTVGPDEVAAAEAVAAARHEAERAAHWAGPGATADGHAVAILANVQDGAAARAARETPAEGVGLF
RTELCFLNRDTEPTVDEQAEIYGEVLDAFAGHKVVVRTLDAGSDKPLKFAGHRDEANPALGVRGIRIAHGNPGILERQLA
GIAAAAARTGTRPWVMAPMIATAGEAKDFAAAARRHGLTPGVMIEVPAAALLADRILEHVDFLSIGTNDLAQYTMAADRM
SADLALLTDPWQPAVLALVAMTVRAGAAAGKPVGVCGEAAADPTLACVLAGLGVTSLSAASAAVPGVGARLAQVTLQQCR
DAAEAVLATATAAEARAAALAALG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=442, Percent_Identity=35.7466063348416, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI48994992, Length=526, Percent_Identity=32.1292775665399, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1788726, Length=528, Percent_Identity=30.3030303030303, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1789193, Length=564, Percent_Identity=28.3687943262411, Blast_Score=188, Evalue=9e-49,
Organism=Escherichia coli, GI1787994, Length=382, Percent_Identity=29.8429319371728, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 59130; Mature: 59130

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGFTPVRVDLSTLFSNLVHMTASSAPTSHATAGTVLRGVPVVPGVQYAPVVRPGRLPVV
CCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
EDTAAEIADADRPAEAARFSAAATAVATRLRDRAAHSTGAASEVLAATATLAQDRAWLGA
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AEKRIAGGTPAVRAVREAVAQFVDLFTKMGGLMAERVTDLQDIRDRVLAELSGLPEPGVP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LPMVPSVLCAEDLAPADTAGLDPTLIVALATTLGGPTSHTAIIARQLGIPCVVAVDGLDA
CCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCC
VPAGAFVLVDGTAGTVTVGPDEVAAAEAVAAARHEAERAAHWAGPGATADGHAVAILANV
CCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
QDGAAARAARETPAEGVGLFRTELCFLNRDTEPTVDEQAEIYGEVLDAFAGHKVVVRTLD
CCCHHHHHHHCCCCCCCCHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
AGSDKPLKFAGHRDEANPALGVRGIRIAHGNPGILERQLAGIAAAAARTGTRPWVMAPMI
CCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHH
ATAGEAKDFAAAARRHGLTPGVMIEVPAAALLADRILEHVDFLSIGTNDLAQYTMAADRM
HHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SADLALLTDPWQPAVLALVAMTVRAGAAAGKPVGVCGEAAADPTLACVLAGLGVTSLSAA
CCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHH
SAAVPGVGARLAQVTLQQCRDAAEAVLATATAAEARAAALAALG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGFTPVRVDLSTLFSNLVHMTASSAPTSHATAGTVLRGVPVVPGVQYAPVVRPGRLPVV
CCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
EDTAAEIADADRPAEAARFSAAATAVATRLRDRAAHSTGAASEVLAATATLAQDRAWLGA
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AEKRIAGGTPAVRAVREAVAQFVDLFTKMGGLMAERVTDLQDIRDRVLAELSGLPEPGVP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LPMVPSVLCAEDLAPADTAGLDPTLIVALATTLGGPTSHTAIIARQLGIPCVVAVDGLDA
CCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCC
VPAGAFVLVDGTAGTVTVGPDEVAAAEAVAAARHEAERAAHWAGPGATADGHAVAILANV
CCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
QDGAAARAARETPAEGVGLFRTELCFLNRDTEPTVDEQAEIYGEVLDAFAGHKVVVRTLD
CCCHHHHHHHCCCCCCCCHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
AGSDKPLKFAGHRDEANPALGVRGIRIAHGNPGILERQLAGIAAAAARTGTRPWVMAPMI
CCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHH
ATAGEAKDFAAAARRHGLTPGVMIEVPAAALLADRILEHVDFLSIGTNDLAQYTMAADRM
HHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SADLALLTDPWQPAVLALVAMTVRAGAAAGKPVGVCGEAAADPTLACVLAGLGVTSLSAA
CCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHH
SAAVPGVGARLAQVTLQQCRDAAEAVLATATAAEARAAALAALG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]