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The map label for this gene is murD

Identifier: 108763559

GI number: 108763559

Start: 6968793

End: 6970160

Strand: Reverse

Name: murD

Synonym: MXAN_5606

Alternate gene names: 108763559

Gene position: 6970160-6968793 (Counterclockwise)

Preceding gene: 108762428

Following gene: 108761950

Centisome position: 76.26

GC content: 72.88

Gene sequence:

>1368_bases
ATGACGTTGGCGCTGTCCGGTCAGAAGGTGCTGGTGTTCGGGCTCGCGAAGAGCGGCGTGGCCGCGCTGCGCCTGCTGCG
TCAGCAGGGCGCGGACGTCACGGCGCTGGACGCACGTGGCGAGGACGCGCTGGGCGCGGTGGCCCACGAGGTGAAGGCGC
TGGGCGCCACGCGGGTGTCCGGGCCCACGCCTCCGGGGCTGCTCGCGTCGCAGGACCTGGTGGTGGTGAGCCCGGGCGTG
CCGCTGGCGCTGCCGGAAATCCAGGCCGCGCGCGCGGCGGGCGTGGCGGTGTGGGGCGAAGTCGAACTGGCGGGCCGCAT
GCTGTCCGGCGTGCCGCTGTTCGGCATCACCGGCACCAACGGCAAGAGCACCACCACGGCGCTCACCGGCACGCTCTTCG
CCAGCGGCGACAAGCGCACCTTCGTGGGCGGAAACCTGGGCCGGCCCTTCAGCGAGGCGGCCATGTCCCCGGGGGACTGG
GACGCGCTGGTGGTGGAGCTGTCCAGCTACCAACTGGAGGGCATCCGCACGCTGCGCCCTCGCGGCGCGGCCATCCTCAA
CCTGACGCCAGACCACATCGACCGCTATCCCAGCCACGCGGCATACGGCGAGGCGAAGGCGCGCATCTTCCAGAACCAGC
AGGCCGGTGACTTCGTGGTGGTGAACGCGGACGACGCGGACGTGCTGGGCCTGGCGCGTGCCGCGAAGGCGCCGGTGTAC
GGCTTCAGCCTCACGGGCAAGCCGGTGGCGGACGCTCCGGCGCTGGCGGGCCTGGCGGTGGTGGAGCCGGGCGGCTTCCG
GCTGGCCTTCCTGGGCGAGCACTACACGCTGACGAACCGCGCGCTGCGCGGCGCCCACAACGCGCAGAACGCGATGGCGG
CGGCGCTGCTGGCTCGCCTGGGCGGCGTGGCCTCCGGCGCGGTGCAGGCGGGGCTGGACGGCTACCCGGGCCTGCCGCAC
CGGCTGGAGAGCGTGCGCGTGCTGGATGGCGTCGAGTGGGTGAATGACTCGAAGGCCACCAACGTGGATTCGGTGCTGGT
GGCGTTGCGCGCCTTCTCCCAAGGCGTGTGGCTGATTGCCGGAGGCAAGGGCAAGGGCGCGCCGTACGCGCCCATGGTGG
AGGCGGGGCAGGGCAAGGTGAAGGGCGTGCTCACCATCGGCGACGACGCGGACACGCTGGCCCGGGCCTATGCCGGCGCG
GCGCAGGTCCACGCGTGCGGCACCCTGGCCCATGCGGTGGCGCGGGCGCGGGAGTTGGCGGAGCGGGGTGACACGGTGCT
GCTGTCGCCCGCGTGCGCGTCCTTCGATCAGTTCAAGAACTTCGAGGACCGGGGCGATTCGTTCAAGCGCCTGGTGGAGG
CGCTGTGA

Upstream 100 bases:

>100_bases
GAGCCGAAGATCATCGTCCGTTTCTGGATCGTCTCCATCCTCTGTGGTGGCGTGGCGCTCCTGTCCCTGAAGCTCCGCTG
AAGCCGGAGGTGGCACGGCC

Downstream 100 bases:

>100_bases
CTGCATGAAGAACCCTTCTCCTCCGTCCTCCGCCCTCGTGCGCTTCGACCCGGTGCTGCTTTGCGCGGTGCTCGGGCTCG
TGAGTTTCGGGCTGGTGATG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTLALSGQKVLVFGLAKSGVAALRLLRQQGADVTALDARGEDALGAVAHEVKALGATRVSGPTPPGLLASQDLVVVSPGV
PLALPEIQAARAAGVAVWGEVELAGRMLSGVPLFGITGTNGKSTTTALTGTLFASGDKRTFVGGNLGRPFSEAAMSPGDW
DALVVELSSYQLEGIRTLRPRGAAILNLTPDHIDRYPSHAAYGEAKARIFQNQQAGDFVVVNADDADVLGLARAAKAPVY
GFSLTGKPVADAPALAGLAVVEPGGFRLAFLGEHYTLTNRALRGAHNAQNAMAAALLARLGGVASGAVQAGLDGYPGLPH
RLESVRVLDGVEWVNDSKATNVDSVLVALRAFSQGVWLIAGGKGKGAPYAPMVEAGQGKVKGVLTIGDDADTLARAYAGA
AQVHACGTLAHAVARARELAERGDTVLLSPACASFDQFKNFEDRGDSFKRLVEAL

Sequences:

>Translated_455_residues
MTLALSGQKVLVFGLAKSGVAALRLLRQQGADVTALDARGEDALGAVAHEVKALGATRVSGPTPPGLLASQDLVVVSPGV
PLALPEIQAARAAGVAVWGEVELAGRMLSGVPLFGITGTNGKSTTTALTGTLFASGDKRTFVGGNLGRPFSEAAMSPGDW
DALVVELSSYQLEGIRTLRPRGAAILNLTPDHIDRYPSHAAYGEAKARIFQNQQAGDFVVVNADDADVLGLARAAKAPVY
GFSLTGKPVADAPALAGLAVVEPGGFRLAFLGEHYTLTNRALRGAHNAQNAMAAALLARLGGVASGAVQAGLDGYPGLPH
RLESVRVLDGVEWVNDSKATNVDSVLVALRAFSQGVWLIAGGKGKGAPYAPMVEAGQGKVKGVLTIGDDADTLARAYAGA
AQVHACGTLAHAVARARELAERGDTVLLSPACASFDQFKNFEDRGDSFKRLVEAL
>Mature_454_residues
TLALSGQKVLVFGLAKSGVAALRLLRQQGADVTALDARGEDALGAVAHEVKALGATRVSGPTPPGLLASQDLVVVSPGVP
LALPEIQAARAAGVAVWGEVELAGRMLSGVPLFGITGTNGKSTTTALTGTLFASGDKRTFVGGNLGRPFSEAAMSPGDWD
ALVVELSSYQLEGIRTLRPRGAAILNLTPDHIDRYPSHAAYGEAKARIFQNQQAGDFVVVNADDADVLGLARAAKAPVYG
FSLTGKPVADAPALAGLAVVEPGGFRLAFLGEHYTLTNRALRGAHNAQNAMAAALLARLGGVASGAVQAGLDGYPGLPHR
LESVRVLDGVEWVNDSKATNVDSVLVALRAFSQGVWLIAGGKGKGAPYAPMVEAGQGKVKGVLTIGDDADTLARAYAGAA
QVHACGTLAHAVARARELAERGDTVLLSPACASFDQFKNFEDRGDSFKRLVEAL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=460, Percent_Identity=35.6521739130435, Blast_Score=205, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_MYXXD (Q1D0S8)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_633745.1
- ProteinModelPortal:   Q1D0S8
- SMR:   Q1D0S8
- STRING:   Q1D0S8
- GeneID:   4107318
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_5606
- TIGR:   MXAN_5606
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q1D0S8
- ProtClustDB:   CLSK946851
- BioCyc:   MXAN246197:MXAN_5606-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 46823; Mature: 46692

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLALSGQKVLVFGLAKSGVAALRLLRQQGADVTALDARGEDALGAVAHEVKALGATRVS
CEEEECCCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCC
GPTPPGLLASQDLVVVSPGVPLALPEIQAARAAGVAVWGEVELAGRMLSGVPLFGITGTN
CCCCCCCCCCCCEEEECCCCCEECCCHHHHHHCCEEEEEHHHHHHHHHCCCEEEEEECCC
GKSTTTALTGTLFASGDKRTFVGGNLGRPFSEAAMSPGDWDALVVELSSYQLEGIRTLRP
CCCCEEEEEEEEEECCCCEEEECCCCCCCHHHHCCCCCCCCEEEEEECCCEECCEEEECC
RGAAILNLTPDHIDRYPSHAAYGEAKARIFQNQQAGDFVVVNADDADVLGLARAAKAPVY
CCCEEEECCHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCEE
GFSLTGKPVADAPALAGLAVVEPGGFRLAFLGEHYTLTNRALRGAHNAQNAMAAALLARL
EEECCCCCCCCCCHHCCEEEECCCCEEEEEECCCEEHHHHHHHCCCHHHHHHHHHHHHHH
GGVASGAVQAGLDGYPGLPHRLESVRVLDGVEWVNDSKATNVDSVLVALRAFSQGVWLIA
CCCCCCHHHCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCEEEEE
GGKGKGAPYAPMVEAGQGKVKGVLTIGDDADTLARAYAGAAQVHACGTLAHAVARARELA
CCCCCCCCCCCHHCCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ERGDTVLLSPACASFDQFKNFEDRGDSFKRLVEAL
HCCCEEEECHHHHCHHHHCCCHHCCHHHHHHHHHC
>Mature Secondary Structure 
TLALSGQKVLVFGLAKSGVAALRLLRQQGADVTALDARGEDALGAVAHEVKALGATRVS
EEEECCCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCC
GPTPPGLLASQDLVVVSPGVPLALPEIQAARAAGVAVWGEVELAGRMLSGVPLFGITGTN
CCCCCCCCCCCCEEEECCCCCEECCCHHHHHHCCEEEEEHHHHHHHHHCCCEEEEEECCC
GKSTTTALTGTLFASGDKRTFVGGNLGRPFSEAAMSPGDWDALVVELSSYQLEGIRTLRP
CCCCEEEEEEEEEECCCCEEEECCCCCCCHHHHCCCCCCCCEEEEEECCCEECCEEEECC
RGAAILNLTPDHIDRYPSHAAYGEAKARIFQNQQAGDFVVVNADDADVLGLARAAKAPVY
CCCEEEECCHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCEE
GFSLTGKPVADAPALAGLAVVEPGGFRLAFLGEHYTLTNRALRGAHNAQNAMAAALLARL
EEECCCCCCCCCCHHCCEEEECCCCEEEEEECCCEEHHHHHHHCCCHHHHHHHHHHHHHH
GGVASGAVQAGLDGYPGLPHRLESVRVLDGVEWVNDSKATNVDSVLVALRAFSQGVWLIA
CCCCCCHHHCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCEEEEE
GGKGKGAPYAPMVEAGQGKVKGVLTIGDDADTLARAYAGAAQVHACGTLAHAVARARELA
CCCCCCCCCCCHHCCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ERGDTVLLSPACASFDQFKNFEDRGDSFKRLVEAL
HCCCEEEECHHHHCHHHHCCCHHCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA